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P13276

- APL3_MANSE

UniProt

P13276 - APL3_MANSE

Protein

Apolipophorin-3

Gene
N/A
Organism
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Assists in the loading of diacylglycerol, generated from triacylglycerol stores in the fat body through the action of adipokinetic hormone, into lipophorin, the hemolymph lipoprotein. It increases the lipid carrying capacity of lipophorin by covering the expanding hydrophobic surface resulting from diacylglycerol uptake. It thus plays a critical role in the transport of lipids during flight in several species of insects.

    GO - Molecular functioni

    1. lipid binding Source: InterPro

    GO - Biological processi

    1. lipid transport Source: UniProtKB-KW

    Keywords - Biological processi

    Lipid transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apolipophorin-3
    Alternative name(s):
    Apolipophorin-III
    Short name:
    ApoLp-III
    OrganismiManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
    Taxonomic identifieri7130 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaSphingidaeSphinginaeSphinginiManduca

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 235PRO_0000002047
    Chaini24 – 189166Apolipophorin-3PRO_0000002048Add
    BLAST

    Keywords - PTMi

    Cleavage on pair of basic residues

    Expressioni

    Tissue specificityi

    Hemolymph.

    Interactioni

    Subunit structurei

    Equilibrium between a soluble monomer and a bound lipoprotein form. Apolipophorin-3 associates with lipophorin during lipid loading until each particle contains 9 or 14 molecules of apolipophorin-3.

    Structurei

    Secondary structure

    1
    189
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi26 – 283
    Helixi34 – 5320
    Helixi64 – 8825
    Helixi95 – 11117
    Helixi112 – 1154
    Helixi118 – 1225
    Beta strandi125 – 1273
    Helixi128 – 15023
    Helixi158 – 1603
    Helixi161 – 18525

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EQ1NMR-A24-189[»]
    ProteinModelPortaliP13276.
    SMRiP13276. Positions 24-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13276.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the insect apolipophorin-3 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    InterProiIPR010009. ApoLp-III.
    [Graphical view]
    PfamiPF07464. ApoLp-III. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13276-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAKFVVVLA ACVALSHSAM VRRDAPAGGN AFEEMEKHAK EFQKTFSEQF    50
    NSLVNSKNTQ DFNKALKDGS DSVLQQLSAF SSSLQGAISD ANGKAKEALE 100
    QARQNVEKTA EELRKAHPDV EKEANAFKDK LQAAVQTTVQ ESQKLAKEVA 150
    SNMEETNKKL APKIKQAYDD FVKHAEEVQK KLHEAATKQ 189
    Length:189
    Mass (Da):20,793
    Last modified:January 1, 1990 - v1
    Checksum:iB1412640AB622169
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17286 mRNA. Translation: AAA29301.1.
    M79326 Genomic DNA. Translation: AAA29300.1.
    PIRiA29793.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Lipid freight - Issue 59 of June 2005

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17286 mRNA. Translation: AAA29301.1 .
    M79326 Genomic DNA. Translation: AAA29300.1 .
    PIRi A29793.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EQ1 NMR - A 24-189 [» ]
    ProteinModelPortali P13276.
    SMRi P13276. Positions 24-189.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P13276.

    Family and domain databases

    InterProi IPR010009. ApoLp-III.
    [Graphical view ]
    Pfami PF07464. ApoLp-III. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and comparative sequence analysis of an insect apolipoprotein. Apolipophorin-III from Manduca sexta."
      Cole K.D., Warnakulasuriya F.G.J.P., Boguski M.S., Freeman M., Gordon J.I., Clark W.A., Law J.H., Wells M.A.
      J. Biol. Chem. 262:11794-11800(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "The structure of the apolipophorin-III gene from Manduca sexta."
      Cole K.D., Smith A.F., Wells M.A.
      Insect Biochem. 20:381-388(1991)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Structural basis for the conformational adaptability of apolipophorin III, a helix-bundle exchangeable apolipoprotein."
      Wang J., Sykes B.D., Ryan R.O.
      Proc. Natl. Acad. Sci. U.S.A. 99:1188-1193(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 24-189.

    Entry informationi

    Entry nameiAPL3_MANSE
    AccessioniPrimary (citable) accession number: P13276
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3