P13276 (APL3_MANSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Apolipophorin-3 Alternative name(s): Apolipophorin-III Short name=ApoLp-III
Organism	Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifier	7130 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Amphiesmenoptera › Lepidoptera › Glossata › Neolepidoptera › Heteroneura › Ditrysia › Obtectomera › Bombycoidea › Sphingidae › Sphinginae › Sphingini › Manduca

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Assists in the loading of diacylglycerol, generated from triacylglycerol stores in the fat body through the action of adipokinetic hormone, into lipophorin, the hemolymph lipoprotein. It increases the lipid carrying capacity of lipophorin by covering the expanding hydrophobic surface resulting from diacylglycerol uptake. It thus plays a critical role in the transport of lipids during flight in several species of insects.
Subunit structure	Equilibrium between a soluble monomer and a bound lipoprotein form. Apolipophorin-3 associates with lipophorin during lipid loading until each particle contains 9 or 14 molecules of apolipophorin-3.
Subcellular location	Secreted.
Tissue specificity	Hemolymph.
Sequence similarities	Belongs to the insect apolipophorin-3 family.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Secreted
Domain	Repeat Signal
PTM	Cleavage on pair of basic residues
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Potential

Propeptide

19 – 23

5

PRO_0000002047

Chain

24 – 189

166

Apolipophorin-3

PRO_0000002048

Secondary structure

1

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189

Helix Strand Turn

Details...

Beta strand

26 – 28

3

Helix

34 – 53

20

Helix

64 – 88

25

Helix

95 – 111

17

Helix

112 – 115

4

Helix

118 – 122

5

Beta strand

125 – 127

3

Helix

128 – 150

23

Helix

158 – 160

3

Helix

161 – 185

25

Sequences

	Sequence		Length	Mass (Da)
	P13276 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: B1412640AB622169	FASTA	189	20,793
	10 20 30 40 50 60 MAAKFVVVLA ACVALSHSAM VRRDAPAGGN AFEEMEKHAK EFQKTFSEQF NSLVNSKNTQ 70 80 90 100 110 120 DFNKALKDGS DSVLQQLSAF SSSLQGAISD ANGKAKEALE QARQNVEKTA EELRKAHPDV 130 140 150 160 170 180 EKEANAFKDK LQAAVQTTVQ ESQKLAKEVA SNMEETNKKL APKIKQAYDD FVKHAEEVQK KLHEAATKQ

References

[1]	"Primary structure and comparative sequence analysis of an insect apolipoprotein. Apolipophorin-III from Manduca sexta." Cole K.D., Warnakulasuriya F.G.J.P., Boguski M.S., Freeman M., Gordon J.I., Clark W.A., Law J.H., Wells M.A. J. Biol. Chem. 262:11794-11800(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The structure of the apolipophorin-III gene from Manduca sexta." Cole K.D., Smith A.F., Wells M.A. Insect Biochem. 20:381-388(1991) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structural basis for the conformational adaptability of apolipophorin III, a helix-bundle exchangeable apolipoprotein." Wang J., Sykes B.D., Ryan R.O. Proc. Natl. Acad. Sci. U.S.A. 99:1188-1193(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 24-189.

Web resources

Protein Spotlight

Lipid freight - Issue 59 of June 2005

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17286 mRNA. Translation: AAA29301.1.
M79326 Genomic DNA. Translation: AAA29300.1.

PIR

A29793.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EQ1	NMR	-	A	24-189	[»]

ProteinModelPortal

P13276.

SMR

P13276. Positions 24-189.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR010009. ApoLp-III.
[Graphical view]

Pfam

PF07464. ApoLp-III. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P13276.

Entry information

Entry name

APL3_MANSE

Accession

Primary (citable) accession number: P13276

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)