Reviewed, UniProtKB/Swiss-Prot P13272 (UCRI_BOVIN)

Names and origin

Protein names

Recommended name:
    Cytochrome b-c1 complex subunit Rieske, mitochondrial
    EC=1.10.2.2
Alternative name(s):
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    Rieske iron-sulfur protein
      Short name=RISP
    Complex III subunit 5
Cleaved into the following chain:
    1- Recommended name:
            Cytochrome b-c1 complex subunit 11
        Alternative name(s):
            Ubiquinol-cytochrome c reductase 8 kDa protein
            Complex III subunit IX

Gene names

Name:

UQCRFS1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit.
Subunit structure	The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.
Sequence caution	The sequence AAA30515.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from sequence or structural similarity. Source: AgBase respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 78

78

Cytochrome b-c1 complex subunit 11

PRO_0000030659

Transit peptide

1 – 78

78

Mitochondrion Ref.5 Ref.9

Chain

79 – 274

196

Cytochrome b-c1 complex subunit Rieske, mitochondrial

PRO_0000030660

Regions

Domain

187 – 272

86

Rieske

Sites

Metal binding

217

1

Iron-sulfur (2Fe-2S)

Metal binding

219

1

Iron-sulfur (2Fe-2S); via pros nitrogen

Metal binding

236

1

Iron-sulfur (2Fe-2S)

Metal binding

239

1

Iron-sulfur (2Fe-2S); via pros nitrogen

Amino acid modifications

Disulfide bond

222 ↔ 238

Ref.6

Experimental info

Sequence conflict

150

1

S → A AA sequence Ref.5

Sequence conflict

269

1

D → G AA sequence Ref.5

Secondary structure

1

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274

Helix Strand Turn

Details...

Turn

4 – 7

4

Beta strand

8 – 10

3

Beta strand

13 – 19

7

Helix

30 – 33

4

Beta strand

34 – 36

3

Beta strand

42 – 46

5

Beta strand

67 – 72

6

Beta strand

74 – 76

3

Helix

80 – 82

3

Turn

89 – 91

3

Beta strand

99 – 101

3

Helix

104 – 138

35

Turn

156 – 158

3

Beta strand

165 – 176

12

Helix

181 – 184

4

Turn

185 – 188

4

Helix

201 – 204

4

Beta strand

205 – 207

3

Helix

208 – 210

3

Beta strand

218 – 221

4

Beta strand

229 – 233

5

Turn

237 – 240

4

Beta strand

251 – 253

3

Beta strand

266 – 271

6

Sequences

	Sequence		Length	Mass (Da)
	P13272-1 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 7C5FC17D2A0DD1C9	FASTA	274	29,547
	10 20 30 40 50 60 MLSVAARSGP FAPVLSATSR GVAGALRPLV QAAVPATSES PVLDLKRSVL CRESLRGQAA 70 80 90 100 110 120 GRPLVASVSL NVPASVRYSH TDIKVPDFSD YRRPEVLDST KSSKESSEAR KGFSYLVTAT 130 140 150 160 170 180 TTVGVAYAAK NVVSQFVSSM SASADVLAMS KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT 190 200 210 220 230 240 KKEIDQEAAV EVSQLRDPQH DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG 250 260 270 SHYDASGRIR KGPAPLNLEV PSYEFTSDDM VIVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a cDNA encoding the Rieske iron-sulfur protein of bovine heart mitochondrial ubiquinol-cytochrome c reductase." Usui S., Yu L., Yu C.-A. Biochem. Biophys. Res. Commun. 167:575-579(1990) [PubMed: 2157409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"The mitochondrial targeting presequence of the Rieske iron-sulfur protein is processed in a single step after insertion into the cytochrome bc1 complex in mammals and retained as a subunit in the complex." Brandt U., Yu L., Yu C.-A., Trumpower B.L. J. Biol. Chem. 268:8387-8390(1993) [PubMed: 8386158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SEQUENCE REVISION. Tissue: Heart.
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus and Hereford. Tissue: Fetal brain, Fetal cerebellum and Liver.
[4]	"Isolation and amino acid sequence of the 8 kDa DCCD-binding protein of beef heart ubiquinol:cytochrome c reductase." Borchart U., Machleidt W., Schagger H., Link T.A., von Jagow G. FEBS Lett. 191:125-130(1985) [PubMed: 2996928] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-78. Tissue: Heart.
[5]	"Isolation and amino acid sequence of the 'Rieske' iron sulfur protein of beef heart ubiquinol:cytochrome c reductase." Schaegger H., Borchart U., Machleidt W., Link T.A., von Jagow G. FEBS Lett. 219:161-168(1987) [PubMed: 3036596] [Abstract] Cited for: PROTEIN SEQUENCE OF 79-274.
[6]	"Structure of a water soluble fragment of the 'Rieske' iron-sulfur protein of the bovine heart mitochondrial cytochrome bc1 complex determined by MAD phasing at 1.5-A resolution." Iwata S., Saynovits M., Link T.A., Michel H. Structure 4:567-579(1996) [PubMed: 8736555] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 148-274.
[7]	"Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria." Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J. Science 277:60-66(1997) [PubMed: 9204897] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
[8]	Erratum Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J. Science 278:2037-2037(1997)
[9]	"Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex." Iwata S., Lee J.W., Okada K., Lee J.K., Iwata M., Rasmussen B., Link T.A., Ramaswamy S., Jap B.K. Science 281:64-71(1998) [PubMed: 9651245] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[10]	"The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition." Gao X., Wen X., Yu C., Esser L., Tsao S., Quinn B., Zhang L., Yu L., Xia D. Biochemistry 41:11692-11702(2002) [PubMed: 12269811] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
[11]	"Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex." Esser L., Quinn B., Li Y.F., Zhang M., Elberry M., Yu L., Yu C.A., Xia D. J. Mol. Biol. 341:281-302(2004) [PubMed: 15312779] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).
[12]	"Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern." Huang L.S., Cobessi D., Tung E.Y., Berry E.A. J. Mol. Biol. 351:573-597(2005) [PubMed: 16024040] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[13]	"Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex." Esser L., Gong X., Yang S., Yu L., Yu C.A., Xia D. Proc. Natl. Acad. Sci. U.S.A. 103:13045-13050(2006) [PubMed: 16924113] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M34336 mRNA. Translation: AAA30515.1. Sequence problems.
S58789 mRNA. Translation: AAB26197.1.
BC115994 mRNA. Translation: AAI15995.1.
BC133620 mRNA. Translation: AAI33621.1.
BC133632 mRNA. Translation: AAI33633.1.

IPI

IPI00688782.

PIR

A34660. A46063.

RefSeq

NP_777238.1.

UniGene

Bt.265

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BCC	X-ray	3.16	E	79-274	[»]
1BE3	X-ray	3.00	E	79-274	[»]
			I	1-78	[»]
1BGY	X-ray	3.00	E/Q	79-274	[»]
			I/U	1-78	[»]
1L0L	X-ray	2.35	E	79-274	[»]
			I	1-78	[»]
1L0N	X-ray	2.60	E	79-274	[»]
			I	1-78	[»]
1NTK	X-ray	2.60	E	79-274	[»]
			I	1-57	[»]
1NTM	X-ray	2.40	E	79-274	[»]
			I	1-57	[»]
1NTZ	X-ray	2.60	E	79-274	[»]
			I	1-57	[»]
1NU1	X-ray	3.20	E	79-274	[»]
			I	1-57	[»]
1PP9	X-ray	2.10	E/R	79-274	[»]
			I/V	1-78	[»]
1PPJ	X-ray	2.10	E/R	79-274	[»]
			I/V	1-78	[»]
1QCR	X-ray	2.70	E	79-274	[»]
			I	21-48	[»]
1RIE	X-ray	1.50	A	146-274	[»]
1SQB	X-ray	2.69	E	79-274	[»]
			I	1-78	[»]
1SQP	X-ray	2.70	E	79-274	[»]
			I	1-78	[»]
1SQQ	X-ray	3.00	E	79-274	[»]
			I	1-78	[»]
1SQV	X-ray	2.85	E	79-274	[»]
			I	1-78	[»]
1SQX	X-ray	2.60	E	79-274	[»]
			I	1-78	[»]
2A06	X-ray	2.10	E/R	79-274	[»]
			I/V	1-78	[»]
2BCC	X-ray	3.50	E	79-274	[»]
2FYU	X-ray	2.26	E	79-274	[»]
			I	1-78	[»]
3BCC	X-ray	3.70	E	79-274	[»]

DisProt

DP00019.

ModBase

Search...

Protein family/group databases

TCDB

3.D.3.2.1. proton-translocating quinol:cytochrome c reductase (QCR) superfamily.

Genome annotation databases

GeneID

287020.

KEGG

bta:287020.

Phylogenomic databases

HOVERGEN

P13272.

Enzyme and pathway databases

BRENDA

1.10.2.2. 251.

Family and domain databases

InterPro

IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

PANTHER

PTHR10134. Rieske. 1 hit.

Pfam

PF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01416. Rieske_proteo. 1 hit.

PROSITE

PS51296. RIESKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UCRI_BOVIN

Accession

Primary (citable) accession number: P13272
Secondary accession number(s): A3KN53, P07588, Q1LZH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)