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Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

UQCRFS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi217Iron-sulfur (2Fe-2S)1
Metal bindingi219Iron-sulfur (2Fe-2S); via pros nitrogen1
Metal bindingi236Iron-sulfur (2Fe-2S)1
Metal bindingi239Iron-sulfur (2Fe-2S); via pros nitrogen1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Protein family/group databases

TCDBi3.D.3.2.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
Alternative name(s):
Complex III subunit 5
Cytochrome b-c1 complex subunit 5
Rieske iron-sulfur protein
Short name:
RISP
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Cleaved into the following chain:
Alternative name(s):
Complex III subunit IX
Ubiquinol-cytochrome c reductase 8 kDa protein
Gene namesi
Name:UQCRFS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei103 – 140HelicalAdd BLAST38

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 78Mitochondrion2 PublicationsAdd BLAST78
ChainiPRO_00000306591 – 78Cytochrome b-c1 complex subunit 11Add BLAST78
ChainiPRO_000003066079 – 274Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi222 ↔ 238PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PeptideAtlasiP13272.
PRIDEiP13272.

Interactioni

Subunit structurei

The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

Protein-protein interaction databases

DIPiDIP-38974N.
IntActiP13272. 3 interactors.

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 7Combined sources3
Beta strandi8 – 10Combined sources3
Beta strandi12 – 19Combined sources8
Beta strandi22 – 24Combined sources3
Beta strandi27 – 29Combined sources3
Helixi30 – 33Combined sources4
Beta strandi34 – 36Combined sources3
Beta strandi43 – 45Combined sources3
Helixi52 – 55Combined sources4
Beta strandi65 – 72Combined sources8
Beta strandi75 – 77Combined sources3
Helixi80 – 82Combined sources3
Turni89 – 91Combined sources3
Helixi94 – 96Combined sources3
Beta strandi99 – 101Combined sources3
Helixi104 – 140Combined sources37
Helixi144 – 147Combined sources4
Beta strandi150 – 155Combined sources6
Helixi156 – 158Combined sources3
Beta strandi164 – 169Combined sources6
Beta strandi172 – 178Combined sources7
Helixi181 – 188Combined sources8
Helixi192 – 194Combined sources3
Beta strandi195 – 197Combined sources3
Helixi201 – 203Combined sources3
Beta strandi205 – 207Combined sources3
Beta strandi210 – 214Combined sources5
Turni218 – 220Combined sources3
Beta strandi225 – 227Combined sources3
Turni229 – 231Combined sources3
Beta strandi232 – 236Combined sources5
Turni237 – 240Combined sources4
Beta strandi241 – 244Combined sources4
Beta strandi245 – 247Combined sources3
Beta strandi249 – 253Combined sources5
Beta strandi263 – 273Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BCCX-ray3.16A192-195[»]
E79-274[»]
1BE3X-ray3.00E79-274[»]
I1-78[»]
1BGYX-ray3.00E/Q79-274[»]
I/U1-78[»]
1L0LX-ray2.35E79-274[»]
I1-78[»]
1L0NX-ray2.60E79-274[»]
I1-78[»]
1NTKX-ray2.60E79-274[»]
I1-57[»]
1NTMX-ray2.40E79-274[»]
I1-57[»]
1NTZX-ray2.60E79-274[»]
I1-57[»]
1NU1X-ray3.20E79-274[»]
I1-57[»]
1PP9X-ray2.10E/R79-274[»]
I/V1-78[»]
1PPJX-ray2.10E/R79-274[»]
I/V1-78[»]
1QCRX-ray2.70E79-274[»]
I21-48[»]
1RIEX-ray1.50A146-274[»]
1SQBX-ray2.69E79-274[»]
I1-78[»]
1SQPX-ray2.70E79-274[»]
I1-78[»]
1SQQX-ray3.00E79-274[»]
I1-78[»]
1SQVX-ray2.85E79-274[»]
I1-78[»]
1SQXX-ray2.60E79-274[»]
I1-78[»]
2A06X-ray2.10E/R79-274[»]
I/V1-78[»]
2FYUX-ray2.26E79-274[»]
I1-78[»]
2YBBelectron microscopy19.00E/e79-274[»]
I/i14-78[»]
4D6TX-ray3.57E/I/R/V1-274[»]
4D6UX-ray4.09E/I/R/V1-274[»]
5KLVX-ray2.65E79-274[»]
I1-78[»]
ProteinModelPortaliP13272.
SMRiP13272.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13272.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 272RieskePROSITE-ProRule annotationAdd BLAST86

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG001040.
InParanoidiP13272.
KOiK00411.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR011070. Globular_prot_asu/bsu.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF56568. SSF56568. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVAARSGP FAPVLSATSR GVAGALRPLV QAAVPATSES PVLDLKRSVL
60 70 80 90 100
CRESLRGQAA GRPLVASVSL NVPASVRYSH TDIKVPDFSD YRRPEVLDST
110 120 130 140 150
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NVVSQFVSSM SASADVLAMS
160 170 180 190 200
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH
210 220 230 240 250
DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR
260 270
KGPAPLNLEV PSYEFTSDDM VIVG
Length:274
Mass (Da):29,547
Last modified:February 1, 1996 - v3
Checksum:i7C5FC17D2A0DD1C9
GO

Sequence cautioni

The sequence AAA30515 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150S → A AA sequence (PubMed:3036596).Curated1
Sequence conflicti269D → G AA sequence (PubMed:3036596).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34336 mRNA. Translation: AAA30515.1. Sequence problems.
S58789 mRNA. Translation: AAB26197.1.
BC115994 mRNA. Translation: AAI15995.1.
BC133620 mRNA. Translation: AAI33621.1.
BC133632 mRNA. Translation: AAI33633.1.
PIRiA46063. A34660.
RefSeqiNP_777238.1. NM_174813.2.
UniGeneiBt.265.

Genome annotation databases

GeneIDi287020.
KEGGibta:287020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34336 mRNA. Translation: AAA30515.1. Sequence problems.
S58789 mRNA. Translation: AAB26197.1.
BC115994 mRNA. Translation: AAI15995.1.
BC133620 mRNA. Translation: AAI33621.1.
BC133632 mRNA. Translation: AAI33633.1.
PIRiA46063. A34660.
RefSeqiNP_777238.1. NM_174813.2.
UniGeneiBt.265.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BCCX-ray3.16A192-195[»]
E79-274[»]
1BE3X-ray3.00E79-274[»]
I1-78[»]
1BGYX-ray3.00E/Q79-274[»]
I/U1-78[»]
1L0LX-ray2.35E79-274[»]
I1-78[»]
1L0NX-ray2.60E79-274[»]
I1-78[»]
1NTKX-ray2.60E79-274[»]
I1-57[»]
1NTMX-ray2.40E79-274[»]
I1-57[»]
1NTZX-ray2.60E79-274[»]
I1-57[»]
1NU1X-ray3.20E79-274[»]
I1-57[»]
1PP9X-ray2.10E/R79-274[»]
I/V1-78[»]
1PPJX-ray2.10E/R79-274[»]
I/V1-78[»]
1QCRX-ray2.70E79-274[»]
I21-48[»]
1RIEX-ray1.50A146-274[»]
1SQBX-ray2.69E79-274[»]
I1-78[»]
1SQPX-ray2.70E79-274[»]
I1-78[»]
1SQQX-ray3.00E79-274[»]
I1-78[»]
1SQVX-ray2.85E79-274[»]
I1-78[»]
1SQXX-ray2.60E79-274[»]
I1-78[»]
2A06X-ray2.10E/R79-274[»]
I/V1-78[»]
2FYUX-ray2.26E79-274[»]
I1-78[»]
2YBBelectron microscopy19.00E/e79-274[»]
I/i14-78[»]
4D6TX-ray3.57E/I/R/V1-274[»]
4D6UX-ray4.09E/I/R/V1-274[»]
5KLVX-ray2.65E79-274[»]
I1-78[»]
ProteinModelPortaliP13272.
SMRiP13272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-38974N.
IntActiP13272. 3 interactors.

Protein family/group databases

TCDBi3.D.3.2.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Proteomic databases

PeptideAtlasiP13272.
PRIDEiP13272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi287020.
KEGGibta:287020.

Organism-specific databases

CTDi7386.

Phylogenomic databases

HOVERGENiHBG001040.
InParanoidiP13272.
KOiK00411.

Miscellaneous databases

EvolutionaryTraceiP13272.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR011070. Globular_prot_asu/bsu.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF56568. SSF56568. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUCRI_BOVIN
AccessioniPrimary (citable) accession number: P13272
Secondary accession number(s): A3KN53, P07588, Q1LZH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.