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P13272

- UCRI_BOVIN

UniProt

P13272 - UCRI_BOVIN

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Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene
UQCRFS1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

Cofactori

Binds 1 2Fe-2S cluster per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi217 – 2171Iron-sulfur (2Fe-2S)
Metal bindingi219 – 2191Iron-sulfur (2Fe-2S); via pros nitrogen
Metal bindingi236 – 2361Iron-sulfur (2Fe-2S)
Metal bindingi239 – 2391Iron-sulfur (2Fe-2S); via pros nitrogen

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. ubiquinol-cytochrome-c reductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Protein family/group databases

TCDBi3.D.3.2.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
Alternative name(s):
Complex III subunit 5
Cytochrome b-c1 complex subunit 5
Rieske iron-sulfur protein
Short name:
RISP
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Cleaved into the following chain:
Alternative name(s):
Complex III subunit IX
Ubiquinol-cytochrome c reductase 8 kDa protein
Gene namesi
Name:UQCRFS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei103 – 14038HelicalAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: AgBase
  3. mitochondrion Source: AgBase
  4. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7878Mitochondrion2 PublicationsAdd
BLAST
Chaini1 – 7878Cytochrome b-c1 complex subunit 11PRO_0000030659Add
BLAST
Chaini79 – 274196Cytochrome b-c1 complex subunit Rieske, mitochondrialPRO_0000030660Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi222 ↔ 2381 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP13272.

Interactioni

Subunit structurei

The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

Protein-protein interaction databases

IntActiP13272. 3 interactions.

Structurei

Secondary structure

1
274
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 73
Beta strandi8 – 103
Beta strandi12 – 198
Beta strandi22 – 243
Beta strandi27 – 293
Helixi30 – 334
Beta strandi34 – 363
Beta strandi43 – 453
Helixi52 – 554
Beta strandi65 – 728
Beta strandi75 – 773
Helixi80 – 823
Turni89 – 913
Helixi94 – 963
Beta strandi99 – 1013
Helixi104 – 14037
Helixi144 – 1474
Beta strandi150 – 1556
Helixi156 – 1583
Beta strandi164 – 1696
Beta strandi172 – 1787
Helixi181 – 1888
Helixi192 – 1943
Beta strandi195 – 1973
Helixi201 – 2033
Beta strandi205 – 2073
Beta strandi210 – 2145
Turni218 – 2203
Beta strandi225 – 2273
Turni229 – 2313
Beta strandi232 – 2365
Turni237 – 2404
Beta strandi241 – 2444
Beta strandi245 – 2473
Beta strandi249 – 2535
Beta strandi263 – 27311

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BCCX-ray3.16A192-195[»]
E79-274[»]
1BE3X-ray3.00E79-274[»]
I1-78[»]
1BGYX-ray3.00E/Q79-274[»]
I/U1-78[»]
1L0LX-ray2.35E79-274[»]
I1-78[»]
1L0NX-ray2.60E79-274[»]
I1-78[»]
1NTKX-ray2.60E79-274[»]
I1-57[»]
1NTMX-ray2.40E79-274[»]
I1-57[»]
1NTZX-ray2.60E79-274[»]
I1-57[»]
1NU1X-ray3.20E79-274[»]
I1-57[»]
1PP9X-ray2.10E/R79-274[»]
I/V1-78[»]
1PPJX-ray2.10E/R79-274[»]
I/V1-78[»]
1QCRX-ray2.70E79-274[»]
I21-48[»]
1RIEX-ray1.50A146-274[»]
1SQBX-ray2.69E79-274[»]
I1-78[»]
1SQPX-ray2.70E79-274[»]
I1-78[»]
1SQQX-ray3.00E79-274[»]
I1-78[»]
1SQVX-ray2.85E79-274[»]
I1-78[»]
1SQXX-ray2.60E79-274[»]
I1-78[»]
2A06X-ray2.10E/R79-274[»]
I/V1-78[»]
2FYUX-ray2.26E79-274[»]
I1-78[»]
2YBBelectron microscopy19.00E/e79-274[»]
I/i14-78[»]
ProteinModelPortaliP13272.
SMRiP13272. Positions 1-57, 79-274.

Miscellaneous databases

EvolutionaryTraceiP13272.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini187 – 27286RieskeAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG001040.
KOiK00411.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR011070. Globular_prot_asu/bsu.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF56568. SSF56568. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13272-1 [UniParc]FASTAAdd to Basket

« Hide

MLSVAARSGP FAPVLSATSR GVAGALRPLV QAAVPATSES PVLDLKRSVL    50
CRESLRGQAA GRPLVASVSL NVPASVRYSH TDIKVPDFSD YRRPEVLDST 100
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NVVSQFVSSM SASADVLAMS 150
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH 200
DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR 250
KGPAPLNLEV PSYEFTSDDM VIVG 274
Length:274
Mass (Da):29,547
Last modified:February 1, 1996 - v3
Checksum:i7C5FC17D2A0DD1C9
GO

Sequence cautioni

The sequence AAA30515.1 differs from that shown. Reason: Frameshift at several positions.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501S → A AA sequence 1 Publication
Sequence conflicti269 – 2691D → G AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34336 mRNA. Translation: AAA30515.1. Sequence problems.
S58789 mRNA. Translation: AAB26197.1.
BC115994 mRNA. Translation: AAI15995.1.
BC133620 mRNA. Translation: AAI33621.1.
BC133632 mRNA. Translation: AAI33633.1.
PIRiA46063. A34660.
RefSeqiNP_777238.1. NM_174813.2.
UniGeneiBt.265.

Genome annotation databases

GeneIDi287020.
KEGGibta:287020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34336 mRNA. Translation: AAA30515.1 . Sequence problems.
S58789 mRNA. Translation: AAB26197.1 .
BC115994 mRNA. Translation: AAI15995.1 .
BC133620 mRNA. Translation: AAI33621.1 .
BC133632 mRNA. Translation: AAI33633.1 .
PIRi A46063. A34660.
RefSeqi NP_777238.1. NM_174813.2.
UniGenei Bt.265.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BCC X-ray 3.16 A 192-195 [» ]
E 79-274 [» ]
1BE3 X-ray 3.00 E 79-274 [» ]
I 1-78 [» ]
1BGY X-ray 3.00 E/Q 79-274 [» ]
I/U 1-78 [» ]
1L0L X-ray 2.35 E 79-274 [» ]
I 1-78 [» ]
1L0N X-ray 2.60 E 79-274 [» ]
I 1-78 [» ]
1NTK X-ray 2.60 E 79-274 [» ]
I 1-57 [» ]
1NTM X-ray 2.40 E 79-274 [» ]
I 1-57 [» ]
1NTZ X-ray 2.60 E 79-274 [» ]
I 1-57 [» ]
1NU1 X-ray 3.20 E 79-274 [» ]
I 1-57 [» ]
1PP9 X-ray 2.10 E/R 79-274 [» ]
I/V 1-78 [» ]
1PPJ X-ray 2.10 E/R 79-274 [» ]
I/V 1-78 [» ]
1QCR X-ray 2.70 E 79-274 [» ]
I 21-48 [» ]
1RIE X-ray 1.50 A 146-274 [» ]
1SQB X-ray 2.69 E 79-274 [» ]
I 1-78 [» ]
1SQP X-ray 2.70 E 79-274 [» ]
I 1-78 [» ]
1SQQ X-ray 3.00 E 79-274 [» ]
I 1-78 [» ]
1SQV X-ray 2.85 E 79-274 [» ]
I 1-78 [» ]
1SQX X-ray 2.60 E 79-274 [» ]
I 1-78 [» ]
2A06 X-ray 2.10 E/R 79-274 [» ]
I/V 1-78 [» ]
2FYU X-ray 2.26 E 79-274 [» ]
I 1-78 [» ]
2YBB electron microscopy 19.00 E/e 79-274 [» ]
I/i 14-78 [» ]
ProteinModelPortali P13272.
SMRi P13272. Positions 1-57, 79-274.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P13272. 3 interactions.

Protein family/group databases

TCDBi 3.D.3.2.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Proteomic databases

PRIDEi P13272.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 287020.
KEGGi bta:287020.

Organism-specific databases

CTDi 7386.

Phylogenomic databases

HOVERGENi HBG001040.
KOi K00411.

Miscellaneous databases

EvolutionaryTracei P13272.
NextBioi 20806543.

Family and domain databases

Gene3Di 1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProi IPR011070. Globular_prot_asu/bsu.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view ]
PANTHERi PTHR10134. PTHR10134. 1 hit.
Pfami PF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view ]
PRINTSi PR00162. RIESKE.
SUPFAMi SSF50022. SSF50022. 1 hit.
SSF56568. SSF56568. 1 hit.
TIGRFAMsi TIGR01416. Rieske_proteo. 1 hit.
PROSITEi PS51296. RIESKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a cDNA encoding the Rieske iron-sulfur protein of bovine heart mitochondrial ubiquinol-cytochrome c reductase."
    Usui S., Yu L., Yu C.-A.
    Biochem. Biophys. Res. Commun. 167:575-579(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  2. "The mitochondrial targeting presequence of the Rieske iron-sulfur protein is processed in a single step after insertion into the cytochrome bc1 complex in mammals and retained as a subunit in the complex."
    Brandt U., Yu L., Yu C.-A., Trumpower B.L.
    J. Biol. Chem. 268:8387-8390(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SEQUENCE REVISION.
    Tissue: Heart.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus and Hereford.
    Tissue: Fetal brain, Fetal cerebellum and Liver.
  4. "Isolation and amino acid sequence of the 8 kDa DCCD-binding protein of beef heart ubiquinol:cytochrome c reductase."
    Borchart U., Machleidt W., Schagger H., Link T.A., von Jagow G.
    FEBS Lett. 191:125-130(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-78.
    Tissue: Heart.
  5. "Isolation and amino acid sequence of the 'Rieske' iron sulfur protein of beef heart ubiquinol:cytochrome c reductase."
    Schaegger H., Borchart U., Machleidt W., Link T.A., von Jagow G.
    FEBS Lett. 219:161-168(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 79-274.
  6. "Structure of a water soluble fragment of the 'Rieske' iron-sulfur protein of the bovine heart mitochondrial cytochrome bc1 complex determined by MAD phasing at 1.5-A resolution."
    Iwata S., Saynovits M., Link T.A., Michel H.
    Structure 4:567-579(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 148-274.
  7. "Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria."
    Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J.
    Science 277:60-66(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
  8. Erratum
    Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J.
    Science 278:2037-2037(1997)
  9. "Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex."
    Iwata S., Lee J.W., Okada K., Lee J.K., Iwata M., Rasmussen B., Link T.A., Ramaswamy S., Jap B.K.
    Science 281:64-71(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
  10. "The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition."
    Gao X., Wen X., Yu C., Esser L., Tsao S., Quinn B., Zhang L., Yu L., Xia D.
    Biochemistry 41:11692-11702(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
  11. "Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex."
    Esser L., Quinn B., Li Y.F., Zhang M., Elberry M., Yu L., Yu C.A., Xia D.
    J. Mol. Biol. 341:281-302(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).
  12. "Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern."
    Huang L.S., Cobessi D., Tung E.Y., Berry E.A.
    J. Mol. Biol. 351:573-597(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
  13. "Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex."
    Esser L., Gong X., Yang S., Yu L., Yu C.A., Xia D.
    Proc. Natl. Acad. Sci. U.S.A. 103:13045-13050(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS).

Entry informationi

Entry nameiUCRI_BOVIN
AccessioniPrimary (citable) accession number: P13272
Secondary accession number(s): A3KN53, P07588, Q1LZH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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