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P13271

- QCR8_BOVIN

UniProt

P13271 - QCR8_BOVIN

Protein

Cytochrome b-c1 complex subunit 8

Gene

UQCRQ

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit, together with cytochrome b, binds to ubiquinone.

    GO - Molecular functioni

    1. ubiquinol-cytochrome-c reductase activity Source: InterPro

    GO - Biological processi

    1. cerebellar Purkinje cell layer development Source: Ensembl
    2. hippocampus development Source: Ensembl
    3. hypothalamus development Source: Ensembl
    4. midbrain development Source: Ensembl
    5. pons development Source: Ensembl
    6. pyramidal neuron development Source: Ensembl
    7. subthalamus development Source: Ensembl
    8. thalamus development Source: Ensembl

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b-c1 complex subunit 8
    Alternative name(s):
    Complex III subunit 8
    Complex III subunit VIII
    Ubiquinol-cytochrome c reductase complex 9.5 kDa protein
    Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C
    Gene namesi
    Name:UQCRQ
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 7

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 8281Cytochrome b-c1 complex subunit 8PRO_0000193543Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei33 – 331N6-acetyllysine; alternateBy similarity
    Modified residuei33 – 331N6-succinyllysine; alternateBy similarity
    Cross-linki33 – 33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    PRIDEiP13271.

    Interactioni

    Subunit structurei

    The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

    Protein-protein interaction databases

    IntActiP13271. 2 interactions.
    STRINGi9913.ENSBTAP00000040860.

    Structurei

    Secondary structure

    1
    82
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 84
    Beta strandi12 – 198
    Turni21 – 233
    Beta strandi26 – 294
    Helixi30 – 6940
    Turni71 – 733
    Turni74 – 763

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BCCX-ray3.16G2-82[»]
    1BE3X-ray3.00G2-82[»]
    1BGYX-ray3.00G/S2-82[»]
    1L0LX-ray2.35G2-82[»]
    1L0NX-ray2.60G2-82[»]
    1NTKX-ray2.60G2-82[»]
    1NTMX-ray2.40G2-82[»]
    1NTZX-ray2.60G2-82[»]
    1NU1X-ray3.20G2-82[»]
    1PP9X-ray2.10G/T2-82[»]
    1PPJX-ray2.10G/T2-82[»]
    1QCRX-ray2.70G2-71[»]
    1SQBX-ray2.69G2-82[»]
    1SQPX-ray2.70G2-82[»]
    1SQQX-ray3.00G2-82[»]
    1SQVX-ray2.85G2-82[»]
    1SQXX-ray2.60G2-82[»]
    2A06X-ray2.10G/T2-82[»]
    2BCCX-ray3.50G2-82[»]
    2FYUX-ray2.26G2-82[»]
    2YBBelectron microscopy19.00G/g2-82[»]
    3BCCX-ray3.70G2-82[»]
    ProteinModelPortaliP13271.
    SMRiP13271. Positions 2-82.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13271.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UQCRQ/QCR8 family.Curated

    Phylogenomic databases

    eggNOGiNOG253869.
    GeneTreeiENSGT00390000004029.
    HOGENOMiHOG000205681.
    HOVERGENiHBG001468.
    InParanoidiP13271.
    KOiK00418.
    OMAiMGREFGH.
    OrthoDBiEOG7K3TPV.
    TreeFamiTF300281.

    Family and domain databases

    Gene3Di1.20.5.210. 1 hit.
    InterProiIPR004205. Cyt_bc1_su8.
    [Graphical view]
    PANTHERiPTHR12119. PTHR12119. 1 hit.
    PfamiPF02939. UcrQ. 1 hit.
    [Graphical view]
    ProDomiPD331499. UcrQ. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF81508. SSF81508. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13271-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRQFGHLTR VRHVITYSLS PFEQRAFPHY FSKGIPNVLR RTRACILRVA   50
    PPFVAFYLVY TWGTQEFEKS KRKNPAAYEN DR 82
    Length:82
    Mass (Da):9,720
    Last modified:January 23, 2007 - v3
    Checksum:i38D7E52051EB7FFA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti62 – 621W → C AA sequence (PubMed:3009231)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06665 mRNA. Translation: AAB03845.1.
    BC103110 mRNA. Translation: AAI03111.1.
    PIRiA24864.
    RefSeqiNP_777230.1. NM_174805.2.
    UniGeneiBt.49658.

    Genome annotation databases

    EnsembliENSBTAT00000043278; ENSBTAP00000040860; ENSBTAG00000009479.
    GeneIDi286885.
    KEGGibta:286885.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06665 mRNA. Translation: AAB03845.1 .
    BC103110 mRNA. Translation: AAI03111.1 .
    PIRi A24864.
    RefSeqi NP_777230.1. NM_174805.2.
    UniGenei Bt.49658.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BCC X-ray 3.16 G 2-82 [» ]
    1BE3 X-ray 3.00 G 2-82 [» ]
    1BGY X-ray 3.00 G/S 2-82 [» ]
    1L0L X-ray 2.35 G 2-82 [» ]
    1L0N X-ray 2.60 G 2-82 [» ]
    1NTK X-ray 2.60 G 2-82 [» ]
    1NTM X-ray 2.40 G 2-82 [» ]
    1NTZ X-ray 2.60 G 2-82 [» ]
    1NU1 X-ray 3.20 G 2-82 [» ]
    1PP9 X-ray 2.10 G/T 2-82 [» ]
    1PPJ X-ray 2.10 G/T 2-82 [» ]
    1QCR X-ray 2.70 G 2-71 [» ]
    1SQB X-ray 2.69 G 2-82 [» ]
    1SQP X-ray 2.70 G 2-82 [» ]
    1SQQ X-ray 3.00 G 2-82 [» ]
    1SQV X-ray 2.85 G 2-82 [» ]
    1SQX X-ray 2.60 G 2-82 [» ]
    2A06 X-ray 2.10 G/T 2-82 [» ]
    2BCC X-ray 3.50 G 2-82 [» ]
    2FYU X-ray 2.26 G 2-82 [» ]
    2YBB electron microscopy 19.00 G/g 2-82 [» ]
    3BCC X-ray 3.70 G 2-82 [» ]
    ProteinModelPortali P13271.
    SMRi P13271. Positions 2-82.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P13271. 2 interactions.
    STRINGi 9913.ENSBTAP00000040860.

    Proteomic databases

    PRIDEi P13271.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000043278 ; ENSBTAP00000040860 ; ENSBTAG00000009479 .
    GeneIDi 286885.
    KEGGi bta:286885.

    Organism-specific databases

    CTDi 27089.

    Phylogenomic databases

    eggNOGi NOG253869.
    GeneTreei ENSGT00390000004029.
    HOGENOMi HOG000205681.
    HOVERGENi HBG001468.
    InParanoidi P13271.
    KOi K00418.
    OMAi MGREFGH.
    OrthoDBi EOG7K3TPV.
    TreeFami TF300281.

    Miscellaneous databases

    EvolutionaryTracei P13271.
    NextBioi 20806531.

    Family and domain databases

    Gene3Di 1.20.5.210. 1 hit.
    InterProi IPR004205. Cyt_bc1_su8.
    [Graphical view ]
    PANTHERi PTHR12119. PTHR12119. 1 hit.
    Pfami PF02939. UcrQ. 1 hit.
    [Graphical view ]
    ProDomi PD331499. UcrQ. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF81508. SSF81508. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, gene sequencing, and expression of the small molecular mass ubiquinone-binding protein of mitochondrial ubiquinol-cytochrome c reductase."
      Yu L., Deng K.-P., Yu C.-A.
      J. Biol. Chem. 270:25634-25638(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Heart ventricle.
    3. "Isolation and amino acid sequence of the 9.5 kDa protein of beef heart ubiquinol:cytochrome c reductase."
      Borchart U., Machleidt W., Schaegger H., Link T.A., von Jagow G.
      FEBS Lett. 200:81-86(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-82.
      Tissue: Heart.
    4. "The small molecular mass ubiquinone-binding protein (QPc-9.5 kDa) in mitochondrial ubiquinol-cytochrome c reductase: isolation, ubiquinone-binding domain, and immunoinhibition."
      Usui S., Yu L., Yu C.A.
      Biochemistry 29:4618-4626(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-14 AND 50-58.
    5. "Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria."
      Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J.
      Science 277:60-66(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
    6. Erratum
      Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J.
      Science 278:2037-2037(1997)
    7. "Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex."
      Iwata S., Lee J.W., Okada K., Lee J.K., Iwata M., Rasmussen B., Link T.A., Ramaswamy S., Jap B.K.
      Science 281:64-71(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
    8. "The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition."
      Gao X., Wen X., Yu C., Esser L., Tsao S., Quinn B., Zhang L., Yu L., Xia D.
      Biochemistry 41:11692-11702(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
    9. "Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex."
      Esser L., Quinn B., Li Y.F., Zhang M., Elberry M., Yu L., Yu C.A., Xia D.
      J. Mol. Biol. 341:281-302(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).
    10. "Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern."
      Huang L.S., Cobessi D., Tung E.Y., Berry E.A.
      J. Mol. Biol. 351:573-597(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
    11. "Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex."
      Esser L., Gong X., Yang S., Yu L., Yu C.A., Xia D.
      Proc. Natl. Acad. Sci. U.S.A. 103:13045-13050(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS).

    Entry informationi

    Entry nameiQCR8_BOVIN
    AccessioniPrimary (citable) accession number: P13271
    Secondary accession number(s): Q3ZBT9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3