P13271 (QCR8_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b-c1 complex subunit 8

Alternative name(s):
Complex III subunit 8
Complex III subunit VIII
Ubiquinol-cytochrome c reductase complex 9.5 kDa protein
Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C

Gene names

Name:

UQCRQ

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	82 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit, together with cytochrome b, binds to ubiquinone.
Subunit structure	The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the UQCRQ/QCR8 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
PTM	Isopeptide bond Ubl conjugation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cerebellar Purkinje cell layer development Inferred from electronic annotation. Source: Compara electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW hippocampus development Inferred from electronic annotation. Source: Compara hypothalamus development Inferred from electronic annotation. Source: Compara midbrain development Inferred from electronic annotation. Source: Compara pons development Inferred from electronic annotation. Source: Compara pyramidal neuron development Inferred from electronic annotation. Source: Compara subthalamus development Inferred from electronic annotation. Source: Compara thalamus development Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3 Ref.4

Chain

2 – 82

81

Cytochrome b-c1 complex subunit 8

PRO_0000193543

Amino acid modifications

Cross-link

33

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict

62

1

W → C AA sequence Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

82

Helix Strand Turn

Details...

Beta strand

5 – 8

4

Beta strand

12 – 19

8

Turn

21 – 23

3

Beta strand

26 – 29

4

Helix

30 – 69

40

Turn

71 – 73

3

Turn

74 – 76

3

Sequences

	Sequence		Length	Mass (Da)
	P13271 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 38D7E52051EB7FFA	FASTA	82	9,720
	10 20 30 40 50 60 MGRQFGHLTR VRHVITYSLS PFEQRAFPHY FSKGIPNVLR RTRACILRVA PPFVAFYLVY 70 80 TWGTQEFEKS KRKNPAAYEN DR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, gene sequencing, and expression of the small molecular mass ubiquinone-binding protein of mitochondrial ubiquinol-cytochrome c reductase." Yu L., Deng K.-P., Yu C.-A. J. Biol. Chem. 270:25634-25638(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.
[3]	"Isolation and amino acid sequence of the 9.5 kDa protein of beef heart ubiquinol:cytochrome c reductase." Borchart U., Machleidt W., Schaegger H., Link T.A., von Jagow G. FEBS Lett. 200:81-86(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-82. Tissue: Heart.
[4]	"The small molecular mass ubiquinone-binding protein (QPc-9.5 kDa) in mitochondrial ubiquinol-cytochrome c reductase: isolation, ubiquinone-binding domain, and immunoinhibition." Usui S., Yu L., Yu C.A. Biochemistry 29:4618-4626(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14 AND 50-58.
[5]	"Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria." Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J. Science 277:60-66(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
[6]	Erratum Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J. Science 278:2037-2037(1997)
[7]	"Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex." Iwata S., Lee J.W., Okada K., Lee J.K., Iwata M., Rasmussen B., Link T.A., Ramaswamy S., Jap B.K. Science 281:64-71(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[8]	"The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition." Gao X., Wen X., Yu C., Esser L., Tsao S., Quinn B., Zhang L., Yu L., Xia D. Biochemistry 41:11692-11702(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
[9]	"Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex." Esser L., Quinn B., Li Y.F., Zhang M., Elberry M., Yu L., Yu C.A., Xia D. J. Mol. Biol. 341:281-302(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).
[10]	"Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern." Huang L.S., Cobessi D., Tung E.Y., Berry E.A. J. Mol. Biol. 351:573-597(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[11]	"Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex." Esser L., Gong X., Yang S., Yu L., Yu C.A., Xia D. Proc. Natl. Acad. Sci. U.S.A. 103:13045-13050(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L06665 mRNA. Translation: AAB03845.1.
BC103110 mRNA. Translation: AAI03111.1.

IPI

IPI00705867.

PIR

A24864.

RefSeq

NP_777230.1. NM_174805.2.

UniGene

Bt.49658.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BCC	X-ray	3.16	G	2-81	[»]
1BE3	X-ray	3.00	G	2-82	[»]
1BGY	X-ray	3.00	G/S	2-82	[»]
1L0L	X-ray	2.35	G	2-82	[»]
1L0N	X-ray	2.60	G	2-82	[»]
1NTK	X-ray	2.60	G	2-81	[»]
1NTM	X-ray	2.40	G	2-81	[»]
1NTZ	X-ray	2.60	G	2-81	[»]
1NU1	X-ray	3.20	G	2-81	[»]
1PP9	X-ray	2.10	G/T	2-82	[»]
1PPJ	X-ray	2.10	G/T	2-82	[»]
1QCR	X-ray	2.70	G	2-71	[»]
1SQB	X-ray	2.69	G	2-82	[»]
1SQP	X-ray	2.70	G	2-82	[»]
1SQQ	X-ray	3.00	G	2-82	[»]
1SQV	X-ray	2.85	G	2-82	[»]
1SQX	X-ray	2.60	G	2-82	[»]
2A06	X-ray	2.10	G/T	2-82	[»]
2BCC	X-ray	3.50	G	2-82	[»]
2FYU	X-ray	2.26	G	2-82	[»]
2YBB	electron microscopy	19.00	G/g	2-82	[»]
3BCC	X-ray	3.70	G	2-81	[»]

ProteinModelPortal

P13271.

SMR

P13271. Positions 2-82.

ModBase

Search...

Protein-protein interaction databases

IntAct

P13271. 2 interactions.

STRING

9913.ENSBTAP00000040860.

Proteomic databases

PRIDE

P13271.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000043278; ENSBTAP00000040860; ENSBTAG00000009479.

GeneID

286885.

KEGG

bta:286885.

Organism-specific databases

CTD

27089.

Phylogenomic databases

eggNOG

NOG253869.

GeneTree

ENSGT00390000004029.

HOGENOM

HOG000205681.

HOVERGEN

HBG001468.

InParanoid

P13271.

KO

K00418.

OMA

VYTWGTQ.

OrthoDB

EOG4PG62J.

Family and domain databases

Gene3D

1.20.5.210. 1 hit.

InterPro

IPR004205. Cyt_bc1_su8.
[Graphical view]

PANTHER

PTHR12119. PTHR12119. 1 hit.

Pfam

PF02939. UcrQ. 1 hit.
[Graphical view]

ProDom

PD331499. UcrQ. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF81508. UcrQ. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P13271.

NextBio

20806531.

Entry information

Entry name

QCR8_BOVIN

Accession

Primary (citable) accession number: P13271
Secondary accession number(s): Q3ZBT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program