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Protein

DNA polymerase III PolC-type

Gene

polC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance, DNA replication

Enzyme and pathway databases

BioCyciBSUB:BSU16580-MONOMER.
SABIO-RKP13267.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III PolC-type (EC:2.7.7.7)
Short name:
PolIII
Gene namesi
Name:polC
Synonyms:dnaF, mutI
Ordered Locus Names:BSU16580
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi427 – 4271E → A: Destroys both polymerase and exonuclease activities. 1 Publication
Mutagenesisi427 – 4271E → Q: Destroys exonuclease activity; decreases polymerase activity. 1 Publication

Chemistry

ChEMBLiCHEMBL3897.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14371437DNA polymerase III PolC-typePRO_0000204573Add
BLAST

Proteomic databases

PaxDbiP13267.

Interactioni

Protein-protein interaction databases

IntActiP13267. 3 interactions.
STRINGi224308.Bsubs1_010100009136.

Chemistry

BindingDBiP13267.

Structurei

3D structure databases

ProteinModelPortaliP13267.
SMRiP13267. Positions 225-403, 420-591, 611-1437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini421 – 578158ExonucleaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni613 – 1437825dNTP polymerization siteSequence analysisAdd
BLAST
Regioni1393 – 143745Contains the active site of the enzymeAdd
BLAST

Sequence similaritiesi

Contains 1 exonuclease domain.Curated

Phylogenomic databases

eggNOGiENOG4105DE5. Bacteria.
COG2176. LUCA.
HOGENOMiHOG000058064.
InParanoidiP13267.
KOiK03763.
OMAiQGCTGVK.
OrthoDBiEOG6ZWJJS.
PhylomeDBiP13267.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_00356. DNApol_PolC.
InterProiIPR011708. DNA_pol3_alpha.
IPR024754. DNA_polC-like_N_II.
IPR028112. DNA_polC-type_N_I.
IPR029460. DNAPol_HHH.
IPR006054. DnaQ.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012340. NA-bd_OB-fold.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR006308. PolC_gram_pos.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF14480. DNA_pol3_a_NI. 1 hit.
PF11490. DNA_pol3_a_NII. 1 hit.
PF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
SM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00573. dnaq. 1 hit.
TIGR01405. polC_Gram_pos. 1 hit.

Sequencei

Sequence statusi: Complete.

P13267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQLSVNRRQ FQILLQQINM TDDTFMTYFE HGEIKKLTIH KASKSWHFHF
60 70 80 90 100
QFKSLLPFQI YDTLTTRLTQ SFAHIAKVTS SIEVQDAEVS ESIVQDYWSR
110 120 130 140 150
CIEELQGISP PIISLLNQQK PKLKGNKLIV KTKTDTEAAA LKNKYSSMIQ
160 170 180 190 200
AEYRQFGFPD LQLDAEIFVS EQEVQKFREQ KLAEDQERAM QALIEMEKKD
210 220 230 240 250
KESDEDQAPS GPLVIGYQIK DNEEIRTLDS IMDEERRITV QGYVFDVETR
260 270 280 290 300
ELKSGRTLCI FKITDYTNSI LIKMFAREKE DAALMKSLKK GMWVKARGSI
310 320 330 340 350
QNDTFVRDLV MIANDVNEIK AKTREDSAPE GEKRVELHLH SPMSQMDAVT
360 370 380 390 400
GIGKLVEQAK KWGHEAIALT DHAVVQSFPD AYSAAKKHGI KMIYGMEANL
410 420 430 440 450
VDDGVPIAYN AAHRLLEEET YVVFDVETTG LSAVYDTIIE LAAVKVKGGE
460 470 480 490 500
IIDKFEAFAN PHRPLSATII ELTGITDDML QDAPDVVDVI RDFREWIGDD
510 520 530 540 550
ILVAHNASFD MGFLNVAYKK LLEVEKAKNP VIDTLELGRF LYPEFKNHRL
560 570 580 590 600
NTLCKKFDIE LTQHHRAIYD TEATAYLLLK MLKDAAEKGI QYHDELNENM
610 620 630 640 650
GQSNAYQRSR PYHATLLAVN STGLKNLFKL VSLSHIHYFY RVPRIPRSQL
660 670 680 690 700
EKYREGLLIG SACDRGEVFE GMMQKSPEEV EDIASFYDYL EVQPPEVYRH
710 720 730 740 750
LLELELVRDE KALKEIIANI TKLGEKLNKP VVATGNVHYL NDEDKIYRKI
760 770 780 790 800
LISSQGGANP LNRHELPKVH FRTTDEMLEA FSFLGEEKAK EIVVTNTQKV
810 820 830 840 850
ASLVDDIKPI KDDLYTPKIE GADEEIREMS YQRARSIYGE ELPEIVEARI
860 870 880 890 900
EKELKSIIGH GFAVIYLISH KLVKRSLDDG YLVGSRGSVG SSLVATLTEI
910 920 930 940 950
TEVNPLPPHY VCPECQHSEF FNDGSVGSGF DLPDKTCPHC GTPLKKDGHD
960 970 980 990 1000
IPFETFLGFK GDKVPDIDLN FSGEYQPQAH NYTKVLFGED NVYRAGTIGT
1010 1020 1030 1040 1050
VAEKTAYGYV KGYAGDNNLH MRGAEIDRLV QGCTGVKRTT GQHPGGIIVV
1060 1070 1080 1090 1100
PDYMDIYDFS PIQFPADATG SEWKTTHFDF HSIHDNLLKL DILGHDDPTV
1110 1120 1130 1140 1150
IRMLQDLSGI DPKTIPTDDP EVMKIFQGTE SLGVTEEQIG CKTGTLGIPE
1160 1170 1180 1190 1200
FGTRFVRQML EDTKPTTFSE LVQISGLSHG TDVWLGNAQE LIHNNICELS
1210 1220 1230 1240 1250
EVIGCRDDIM VYLIYQGLEP SLAFKIMEFV RKGKGLTPEW EEEMKNNNVP
1260 1270 1280 1290 1300
DWYIDSCKKI KYMFPKAHAA AYVLMAVRIA YFKVHHALLY YAAYFTVRAD
1310 1320 1330 1340 1350
DFDIDTMIKG STAIRAVMED INAKGLDASP KEKNLLTVLE LALEMCERGY
1360 1370 1380 1390 1400
SFQKVDLYRS SATEFIIDGN SLIPPFNSIP GLGTNAALNI VKAREEGEFL
1410 1420 1430
SKEDLQKRGK VSKTILEYLD RHGCLESLPD QNQLSLF
Length:1,437
Mass (Da):162,663
Last modified:August 1, 1991 - v2
Checksum:i0C04FC12D08C2E74
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1885EDQER → KTKSW in AAA22666 (PubMed:2499883).Curated
Sequence conflicti495 – 4962EW → VR in AAA22666 (PubMed:2499883).Curated
Sequence conflicti829 – 8291M → S in AAA22666 (PubMed:2499883).Curated
Sequence conflicti1015 – 10151G → E in AAA22666 (PubMed:2499883).Curated
Sequence conflicti1190 – 11901E → D in AAA22666 (PubMed:2499883).Curated
Sequence conflicti1405 – 14062LQ → FE in AAA22666 (PubMed:2499883).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1175 – 11751S → A in mutant AZP12.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52116 Genomic DNA. Translation: CAA36362.1.
M22996 Genomic DNA. Translation: AAA22666.1.
AL009126 Genomic DNA. Translation: CAB13531.1.
S55653 Genomic DNA. Translation: AAP13898.1.
M33543 Genomic DNA. Translation: AAA22667.1.
Z18631 Genomic DNA. No translation available.
PIRiF69680.
RefSeqiNP_389540.1. NC_000964.3.
WP_003245843.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13531; CAB13531; BSU16580.
GeneIDi939620.
KEGGibsu:BSU16580.
PATRICi18975123. VBIBacSub10457_1753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52116 Genomic DNA. Translation: CAA36362.1.
M22996 Genomic DNA. Translation: AAA22666.1.
AL009126 Genomic DNA. Translation: CAB13531.1.
S55653 Genomic DNA. Translation: AAP13898.1.
M33543 Genomic DNA. Translation: AAA22667.1.
Z18631 Genomic DNA. No translation available.
PIRiF69680.
RefSeqiNP_389540.1. NC_000964.3.
WP_003245843.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP13267.
SMRiP13267. Positions 225-403, 420-591, 611-1437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13267. 3 interactions.
STRINGi224308.Bsubs1_010100009136.

Chemistry

BindingDBiP13267.
ChEMBLiCHEMBL3897.

Proteomic databases

PaxDbiP13267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13531; CAB13531; BSU16580.
GeneIDi939620.
KEGGibsu:BSU16580.
PATRICi18975123. VBIBacSub10457_1753.

Phylogenomic databases

eggNOGiENOG4105DE5. Bacteria.
COG2176. LUCA.
HOGENOMiHOG000058064.
InParanoidiP13267.
KOiK03763.
OMAiQGCTGVK.
OrthoDBiEOG6ZWJJS.
PhylomeDBiP13267.

Enzyme and pathway databases

BioCyciBSUB:BSU16580-MONOMER.
SABIO-RKP13267.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_00356. DNApol_PolC.
InterProiIPR011708. DNA_pol3_alpha.
IPR024754. DNA_polC-like_N_II.
IPR028112. DNA_polC-type_N_I.
IPR029460. DNAPol_HHH.
IPR006054. DnaQ.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012340. NA-bd_OB-fold.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR006308. PolC_gram_pos.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF14480. DNA_pol3_a_NI. 1 hit.
PF11490. DNA_pol3_a_NII. 1 hit.
PF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
SM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00573. dnaq. 1 hit.
TIGR01405. polC_Gram_pos. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bacillus subtilis DNA polymerase III: complete sequence, overexpression, and characterization of the polC gene."
    Hammond R.A., Barnes M.H., Mack S.L., Mitchener J.A., Brown N.C.
    Gene 98:29-36(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: 168 / BD541.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SB19.
  3. "Genetic structure and domains of DNA polymerase III of Bacillus subtilis."
    Sanjanwala B., Ganesan A.T.
    Mol. Gen. Genet. 226:467-472(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "Leader region of the gene encoding DNA polymerase III of Bacillus subtilis."
    Sanjanwala B., Ganesan A.T.
    Mol. Gen. Genet. 236:374-378(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
    Strain: SG64.
  6. "The cloned polC gene of Bacillus subtilis: characterization of the azp12 mutation and controlled in vitro synthesis of active DNA polymerase III."
    Barnes M.H., Hammond R.A., Foster K.A., Mitchener J.A., Brown N.C.
    Gene 85:177-186(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1150-1229.
  7. "Similar organization of the nusA-infB operon in Bacillus subtilis and Escherichia coli."
    Shazand K., Tucker J., Grunberg-Manago M., Rabinowitz J.C., Leighton T.
    J. Bacteriol. 175:2880-2887(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1398-1437.
  8. "Localization of the exonuclease and polymerase domains of Bacillus subtilis DNA polymerase III."
    Barnes M.H., Hammond R.A., Kennedy C.S., Mack S.L., Brown N.C.
    Gene 111:43-49(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiDPO3_BACSU
AccessioniPrimary (citable) accession number: P13267
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: August 1, 1991
Last modified: February 17, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutant azp12 has a form of DNA polymerase III resistant to hydroxyphenylazopyrimidines.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.