P13265 (GPC3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glypican-3

Alternative name(s):
Intestinal protein OCI-5

Gene names

Name:

Gpc3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the dipeptidyl peptidase activity of DPP4 By similarity. Cell surface proteoglycan that bears heparan sulfate. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Subunit structure	Interacts with DPP4 By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side. Secreted.
Tissue specificity	Fetal tissues and adult lung.
Sequence similarities	Belongs to the glypican family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	Signal
Molecular function	Protease inhibitor
PTM	GPI-anchor Glycoprotein Heparan sulfate Lipoprotein Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of canonical Wnt receptor signaling pathway Inferred from direct assay. Source: MGI positive regulation of Wnt receptor signaling pathway, planar cell polarity pathway Inferred from direct assay. Source: MGI
Cellular component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: InterPro
Molecular function	heparan sulfate proteoglycan binding Inferred from electronic annotation. Source: InterPro peptidyl-dipeptidase inhibitor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – ?		Glypican-3		PRO_0000012313
	Propeptide	? – 597		Removed in mature form Potential		PRO_0000012314
Amino acid modifications
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	417	1	N-linked (GlcNAc...) Potential
	Glycosylation	494	1	O-linked (Xyl...) (heparan sulfate) Potential
	Glycosylation	508	1	O-linked (Xyl...) (heparan sulfate) Potential
Experimental info
	Sequence conflict	553	1	S → N in AAH85756. Ref.2
	Sequence conflict	583 – 584	2	CP → VSQ in AAH85756. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P13265 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: C556AD4127685687	FASTA	597	67,049
	10 20 30 40 50 60 MAGTVRTACL LVAMLLGLGC LGQAQPPPPP DATCHQVRSF FQRLQPGLKW VPETPVPGSD 70 80 90 100 110 120 LQVCLPKGPT CCSRKMEEKY QLTARLNMEQ LLQSASMELK FLIIQNAAVF QEAFEIVVRH 130 140 150 160 170 180 AKNYTNAMFK NNYPSLTPQA FEFVGEFFTD VSLYILGSDI NVDDMVNELF DSLFPVIYTQ 190 200 210 220 230 240 MMNPGLPESV LDINECLRGA RRDLKVFGSF PKLIMTQVSK SLQVTRIFLQ ALNLGIEVIN 250 260 270 280 290 300 TTDHLKFSKD CGRMLTRMWY CSYCQGLMMV KPCGGYCNVV MQGCMAGVVE IDKYWREYIL 310 320 330 340 350 360 SLEELVNGMY RIYDMENVLL GLFSTIHDSI QYVQKNGGKL TTTIGKLCAH SQQRQYRSAY 370 380 390 400 410 420 YPEDLFIDKK VLKVARVEHE ETLSSRRREL IQKLKSFISF YSALPGYICS HSPVAENDTL 430 440 450 460 470 480 CWNGQELVER YSQKAARNGM KNQFNLHELK MKGPEPVVSQ IIDKLKHINQ LLRTMSVPKG 490 500 510 520 530 540 KVVDKSLDEE GLESGDCGDD EDECIGSSGD GMMKVKNQLR FLAELAYDLD VDDAPGNKQH 550 560 570 580 590 GNQKDNEITT SHSVGNMPSP LKILISVAIY VACFFSWCTD LPCPCLCCPA APCGPPT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a cDNA corresponding to a developmentally regulated transcript in rat intestine." Filmus J., Church J.G., Buick R.N. Mol. Cell. Biol. 8:4243-4249(1988) [PubMed: 3185547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Identification of a new membrane-bound heparan sulphate proteoglycan." Filmus J., Shi W., Wong Z.M., Wong M.J. Biochem. J. 311:561-565(1995) [PubMed: 7487896] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22400 mRNA. Translation: AAA41735.1. BC085756 mRNA. Translation: AAH85756.1.
IPI	IPI00199466.
PIR	A30814.
RefSeq	NP_036906.1. NM_012774.1.
UniGene	Rn.9717.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P13265.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25236.
KEGG	rno:25236.
Organism-specific databases
CTD	2719.
RGD	2725. Gpc3.
Phylogenomic databases
eggNOG	roNOG08068.
GeneTree	ENSGT00550000074430.
HOVERGEN	HBG005896.
Gene expression databases
ArrayExpress	P13265.
Genevestigator	P13265.
GermOnline	ENSRNOG00000022684. Rattus norvegicus.
Family and domain databases
InterPro	IPR001863. Glypican. IPR015501. Glypican-3. IPR019803. Glypican_CS. [Graphical view]
KO	K08109.
PANTHER	PTHR10822. Glypican. 1 hit. PTHR10822:SF4. GPC3. 1 hit.
Pfam	PF01153. Glypican. 1 hit. [Graphical view]
PROSITE	PS01207. GLYPICAN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	605807.

Entry information

Entry name

GPC3_RAT

Accession

Primary (citable) accession number: P13265
Secondary accession number(s): Q5U326

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program