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Protein

Glutaminase kidney isoform, mitochondrial

Gene

Gls

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei291SubstrateBy similarity1
Binding sitei340SubstrateBy similarity1
Binding sitei386SubstrateBy similarity1
Binding sitei393SubstrateBy similarity1
Binding sitei419SubstrateBy similarity1
Binding sitei471SubstrateBy similarity1
Binding sitei489Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.5.1.2. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase kidney isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Alternative name(s):
K-glutaminase
L-glutamine amidohydrolase
Cleaved into the following 2 chains:
Gene namesi
Name:Gls
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2707. Gls.

Subcellular locationi

Isoform 1 :

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • mitochondrial matrix Source: GO_Central
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 16MitochondrionAdd BLAST16
ChainiPRO_000001162317 – 674Glutaminase kidney isoform 68 kDa chainAdd BLAST658
ChainiPRO_000001162473 – 674Glutaminase kidney isoform 65 kDa chainAdd BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135N6-succinyllysineBy similarity1
Modified residuei169N6-succinyllysineBy similarity1
Modified residuei316N6-acetyllysineBy similarity1
Modified residuei657PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP13264.
PRIDEiP13264.

PTM databases

iPTMnetiP13264.
PhosphoSitePlusiP13264.

Expressioni

Tissue specificityi

Kidney, brain, and intestine.

Interactioni

Subunit structurei

Homotetramer. Tetramer composed of 68 and 65 kDa peptides in a 1:3 ratio. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.1 Publication

Protein-protein interaction databases

DIPiDIP-60007N.
IntActiP13264. 1 interactor.
STRINGi10116.ENSRNOP00000038205.

Structurei

3D structure databases

ProteinModelPortaliP13264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati590 – 619ANK 1Add BLAST30
Repeati624 – 653ANK 2Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi91 – 99Poly-Gln9

Sequence similaritiesi

Belongs to the glutaminase family.Curated
Contains 2 ANK repeats.Curated

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0506. Eukaryota.
COG2066. LUCA.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiP13264.
KOiK01425.
PhylomeDBiP13264.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P13264-1) [UniParc]FASTAAdd to basket
Also known as: KGA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRLRGSAML RELLLRPPAA VGGVLRRTQP LGTLCRRPRG GSRPAAGLVA
60 70 80 90 100
AARLHPWWGG GGRAKGPGSG GLSSSPSEIL QELGKGGTPP QQQQQQQQQP
110 120 130 140 150
GASPPAAPGP KDSPGETDAF GNSEGKEMVA AGDNKVKQGL LPSLEDLLFY
160 170 180 190 200
TIAEGQEKIP VHKFITALKS TGLRTSDPRL KECMDMLRLT LQTTSDGVML
210 220 230 240 250
DKDLFKKCVQ SNIVLLTQAF RRKFVIPDFM SFTSHIDELY ESAKKQSGGK
260 270 280 290 300
VADYIPQLAK FSPDLWGVSV CTVDGQRHSI GDTKVPFCLQ SCVKPLKYAI
310 320 330 340 350
AVNDLGTEYV HRYVGKEPSG LRFNKLFLNE DDKPHNPMVN AGAIVVTSLI
360 370 380 390 400
KQGVNNAEKF DYVMQFLNKM AGNEYVGFSN ATFQSERESG DRNFAIGYYL
410 420 430 440 450
KEKKCFPEGT DMVGILDFYF QLCSIEVTCE SASVMAATLA NGGFCPITGE
460 470 480 490 500
RVLSPEAVRN TLSLMHSCGM YDFSGQFAFH VGLPAKSGVA GGILLVVPNV
510 520 530 540 550
MGMMCWSPPL DKMGNSVKGI HFCHDLVSLC NFHNYDNLRH FAKKLDPRRE
560 570 580 590 600
GGDQRVKSVI NLLFAAYTGD VSALRRFALS AMDMEQRDYD SRTALHVAAA
610 620 630 640 650
EGHVEVVKFL LEACKVNPFP KDRWNNTPMD EALHFGHHDV FKILQEYQVQ
660 670
YTPQGDSDDG KENQTVHKNL DGLL
Length:674
Mass (Da):74,024
Last modified:December 1, 1992 - v2
Checksum:i4B2524A45D3678CA
GO
Isoform 2 (identifier: P13264-2) [UniParc]FASTAAdd to basket
Also known as: GAC

The sequence of this isoform differs from the canonical sequence as follows:
     556-674: VKSVINLLFA...TVHKNLDGLL → HSFGPLDYES...YRMESLGERS

Show »
Length:603
Mass (Da):65,997
Checksum:iF4DD80AA26873041
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28T → A in AAG30873 (PubMed:11267668).Curated1
Sequence conflicti349 – 351LIK → EFG in AAA41234 (PubMed:3401701).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041992556 – 674VKSVI…LDGLL → HSFGPLDYESLQQELALKDT VWKKVSPESSDDTSTTIVYR MESLGERS in isoform 2. 1 PublicationAdd BLAST119

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65150 mRNA. Translation: AAA41247.1.
AF302091 Genomic DNA. Translation: AAG30873.1.
AY083459 mRNA. Translation: AAM00020.1.
M22586 mRNA. Translation: AAA41234.1.
PIRiA41009.
RefSeqiNP_001103438.1. NM_001109968.1.
NP_036701.2. NM_012569.2.
UniGeneiRn.5762.

Genome annotation databases

GeneIDi24398.
KEGGirno:24398.
UCSCiRGD:2707. rat. [P13264-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65150 mRNA. Translation: AAA41247.1.
AF302091 Genomic DNA. Translation: AAG30873.1.
AY083459 mRNA. Translation: AAM00020.1.
M22586 mRNA. Translation: AAA41234.1.
PIRiA41009.
RefSeqiNP_001103438.1. NM_001109968.1.
NP_036701.2. NM_012569.2.
UniGeneiRn.5762.

3D structure databases

ProteinModelPortaliP13264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60007N.
IntActiP13264. 1 interactor.
STRINGi10116.ENSRNOP00000038205.

PTM databases

iPTMnetiP13264.
PhosphoSitePlusiP13264.

Proteomic databases

PaxDbiP13264.
PRIDEiP13264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24398.
KEGGirno:24398.
UCSCiRGD:2707. rat. [P13264-1]

Organism-specific databases

CTDi2744.
RGDi2707. Gls.

Phylogenomic databases

eggNOGiKOG0506. Eukaryota.
COG2066. LUCA.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiP13264.
KOiK01425.
PhylomeDBiP13264.

Enzyme and pathway databases

BRENDAi3.5.1.2. 5301.

Miscellaneous databases

PROiP13264.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLSK_RAT
AccessioniPrimary (citable) accession number: P13264
Secondary accession number(s): Q8R421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.