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P13264

- GLSK_RAT

UniProt

P13264 - GLSK_RAT

Protein

Glutaminase kidney isoform, mitochondrial

Gene

Gls

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.

    Catalytic activityi

    L-glutamine + H2O = L-glutamate + NH3.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei291 – 2911SubstrateBy similarity
    Binding sitei340 – 3401SubstrateBy similarity
    Binding sitei386 – 3861SubstrateBy similarity
    Binding sitei393 – 3931SubstrateBy similarity
    Binding sitei419 – 4191SubstrateBy similarity
    Binding sitei471 – 4711SubstrateBy similarity
    Binding sitei489 – 4891Substrate; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. glutaminase activity Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. glutamate biosynthetic process Source: UniProtKB
    2. glutamine catabolic process Source: UniProtKB
    3. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    SABIO-RKP13264.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase kidney isoform, mitochondrial (EC:3.5.1.2)
    Short name:
    GLS
    Alternative name(s):
    K-glutaminase
    L-glutamine amidohydrolase
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Gls
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2707. Gls.

    Subcellular locationi

    Isoform 1 : Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1616MitochondrionAdd
    BLAST
    Chaini17 – 674658Glutaminase kidney isoform 68 kDa chainPRO_0000011623Add
    BLAST
    Chaini73 – 674602Glutaminase kidney isoform 65 kDa chainPRO_0000011624Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei135 – 1351N6-succinyllysineBy similarity
    Modified residuei169 – 1691N6-succinyllysineBy similarity
    Modified residuei316 – 3161N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP13264.
    PRIDEiP13264.

    PTM databases

    PhosphoSiteiP13264.

    Expressioni

    Tissue specificityi

    Kidney, brain, and intestine.

    Gene expression databases

    GenevestigatoriP13264.

    Interactioni

    Subunit structurei

    Homotetramer. Tetramer composed of 68 and 65 kDa peptides in a 1:3 ratio. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.1 Publication

    Protein-protein interaction databases

    DIPiDIP-60007N.
    IntActiP13264. 1 interaction.
    STRINGi10116.ENSRNOP00000063176.

    Structurei

    3D structure databases

    ProteinModelPortaliP13264.
    SMRiP13264. Positions 227-536.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati590 – 61930ANK 1Add
    BLAST
    Repeati624 – 65330ANK 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi91 – 999Poly-Gln

    Sequence similaritiesi

    Belongs to the glutaminase family.Curated
    Contains 2 ANK repeats.Curated

    Keywords - Domaini

    ANK repeat, Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG2066.
    HOGENOMiHOG000216891.
    HOVERGENiHBG005856.
    InParanoidiQ8R421.
    KOiK01425.
    PhylomeDBiP13264.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF00023. Ank. 1 hit.
    PF04960. Glutaminase. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P13264-1) [UniParc]FASTAAdd to Basket

    Also known as: KGA

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMRLRGSAML RELLLRPPAA VGGVLRRTQP LGTLCRRPRG GSRPAAGLVA    50
    AARLHPWWGG GGRAKGPGSG GLSSSPSEIL QELGKGGTPP QQQQQQQQQP 100
    GASPPAAPGP KDSPGETDAF GNSEGKEMVA AGDNKVKQGL LPSLEDLLFY 150
    TIAEGQEKIP VHKFITALKS TGLRTSDPRL KECMDMLRLT LQTTSDGVML 200
    DKDLFKKCVQ SNIVLLTQAF RRKFVIPDFM SFTSHIDELY ESAKKQSGGK 250
    VADYIPQLAK FSPDLWGVSV CTVDGQRHSI GDTKVPFCLQ SCVKPLKYAI 300
    AVNDLGTEYV HRYVGKEPSG LRFNKLFLNE DDKPHNPMVN AGAIVVTSLI 350
    KQGVNNAEKF DYVMQFLNKM AGNEYVGFSN ATFQSERESG DRNFAIGYYL 400
    KEKKCFPEGT DMVGILDFYF QLCSIEVTCE SASVMAATLA NGGFCPITGE 450
    RVLSPEAVRN TLSLMHSCGM YDFSGQFAFH VGLPAKSGVA GGILLVVPNV 500
    MGMMCWSPPL DKMGNSVKGI HFCHDLVSLC NFHNYDNLRH FAKKLDPRRE 550
    GGDQRVKSVI NLLFAAYTGD VSALRRFALS AMDMEQRDYD SRTALHVAAA 600
    EGHVEVVKFL LEACKVNPFP KDRWNNTPMD EALHFGHHDV FKILQEYQVQ 650
    YTPQGDSDDG KENQTVHKNL DGLL 674
    Length:674
    Mass (Da):74,024
    Last modified:December 1, 1992 - v2
    Checksum:i4B2524A45D3678CA
    GO
    Isoform 2 (identifier: P13264-2) [UniParc]FASTAAdd to Basket

    Also known as: GAC

    The sequence of this isoform differs from the canonical sequence as follows:
         556-674: VKSVINLLFA...TVHKNLDGLL → HSFGPLDYES...YRMESLGERS

    Show »
    Length:603
    Mass (Da):65,997
    Checksum:iF4DD80AA26873041
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281T → A in AAG30873. (PubMed:11267668)Curated
    Sequence conflicti349 – 3513LIK → EFG in AAA41234. (PubMed:3401701)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei556 – 674119VKSVI…LDGLL → HSFGPLDYESLQQELALKDT VWKKVSPESSDDTSTTIVYR MESLGERS in isoform 2. 1 PublicationVSP_041992Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65150 mRNA. Translation: AAA41247.1.
    AF302091 Genomic DNA. Translation: AAG30873.1.
    AY083459 mRNA. Translation: AAM00020.1.
    M22586 mRNA. Translation: AAA41234.1.
    PIRiA41009.
    RefSeqiNP_001103438.1. NM_001109968.1.
    NP_036701.2. NM_012569.2.
    UniGeneiRn.5762.

    Genome annotation databases

    GeneIDi24398.
    KEGGirno:24398.
    UCSCiRGD:2707. rat. [P13264-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65150 mRNA. Translation: AAA41247.1 .
    AF302091 Genomic DNA. Translation: AAG30873.1 .
    AY083459 mRNA. Translation: AAM00020.1 .
    M22586 mRNA. Translation: AAA41234.1 .
    PIRi A41009.
    RefSeqi NP_001103438.1. NM_001109968.1.
    NP_036701.2. NM_012569.2.
    UniGenei Rn.5762.

    3D structure databases

    ProteinModelPortali P13264.
    SMRi P13264. Positions 227-536.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60007N.
    IntActi P13264. 1 interaction.
    STRINGi 10116.ENSRNOP00000063176.

    PTM databases

    PhosphoSitei P13264.

    Proteomic databases

    PaxDbi P13264.
    PRIDEi P13264.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24398.
    KEGGi rno:24398.
    UCSCi RGD:2707. rat. [P13264-1 ]

    Organism-specific databases

    CTDi 2744.
    RGDi 2707. Gls.

    Phylogenomic databases

    eggNOGi COG2066.
    HOGENOMi HOG000216891.
    HOVERGENi HBG005856.
    InParanoidi Q8R421.
    KOi K01425.
    PhylomeDBi P13264.

    Enzyme and pathway databases

    SABIO-RK P13264.

    Miscellaneous databases

    NextBioi 603191.
    PROi P13264.

    Gene expression databases

    Genevestigatori P13264.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    3.40.710.10. 1 hit.
    HAMAPi MF_00313. Glutaminase.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view ]
    PANTHERi PTHR12544. PTHR12544. 1 hit.
    Pfami PF00023. Ank. 1 hit.
    PF04960. Glutaminase. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF56601. SSF56601. 1 hit.
    TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization, and in vitro expression of a cDNA that encodes the kidney isoenzyme of the mitochondrial glutaminase."
      Shapiro R.A., Farrell L., Srinivasan M., Curthoys N.P.
      J. Biol. Chem. 266:18792-18796(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 73-90.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. "Isolation and characterization of the promoter region of the rat kidney-type glutaminase gene."
      Taylor L., Liu X., Newsome W., Shapiro R.A., Srinivasan M., Curthoys N.P.
      Biochim. Biophys. Acta 1518:132-136(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-133.
      Strain: Sprague-Dawley.
    3. "Complexity and species variation of the kidney-type glutaminase gene."
      Porter L.D., Ibrahim H., Taylor L., Curthoys N.P.
      Physiol. Genomics 9:157-166(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 299-674 (ISOFORM 2).
    4. "Isolation of a cDNA for rat brain glutaminase."
      Banner C., Hwang J.-J., Shapiro R.A., Wenthold R.J., Nakatani Y., Lample K.A., Thomas J.W., Huie D., Curthoys N.P.
      Brain Res. 427:247-254(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 349-651 (ISOFORM 1).
      Tissue: Brain.
    5. "Biosynthesis and processing of mitochondrial glutaminase in HTC hepatoma cells."
      Perera S.Y., Voith D.M., Curthoys N.P.
      Biochem. J. 273:265-270(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING.
    6. "Brain-specific BNIP-2-homology protein Caytaxin relocalises glutaminase to neurite terminals and reduces glutamate levels."
      Buschdorf J.P., Li Chew L., Zhang B., Cao Q., Liang F.Y., Liou Y.C., Zhou Y.T., Low B.C.
      J. Cell Sci. 119:3337-3350(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH ATCAY.

    Entry informationi

    Entry nameiGLSK_RAT
    AccessioniPrimary (citable) accession number: P13264
    Secondary accession number(s): Q8R421
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3