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P13264

- GLSK_RAT

UniProt

P13264 - GLSK_RAT

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Protein

Glutaminase kidney isoform, mitochondrial

Gene

Gls

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei291 – 2911SubstrateBy similarity
Binding sitei340 – 3401SubstrateBy similarity
Binding sitei386 – 3861SubstrateBy similarity
Binding sitei393 – 3931SubstrateBy similarity
Binding sitei419 – 4191SubstrateBy similarity
Binding sitei471 – 4711SubstrateBy similarity
Binding sitei489 – 4891Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB

GO - Biological processi

  1. glutamate biosynthetic process Source: UniProtKB
  2. glutamine catabolic process Source: UniProtKB
  3. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

SABIO-RKP13264.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase kidney isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Alternative name(s):
K-glutaminase
L-glutamine amidohydrolase
Cleaved into the following 2 chains:
Gene namesi
Name:Gls
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2707. Gls.

Subcellular locationi

Isoform 1 : Cytoplasmcytosol By similarity

GO - Cellular componenti

  1. mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1616MitochondrionAdd
BLAST
Chaini17 – 674658Glutaminase kidney isoform 68 kDa chainPRO_0000011623Add
BLAST
Chaini73 – 674602Glutaminase kidney isoform 65 kDa chainPRO_0000011624Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei135 – 1351N6-succinyllysineBy similarity
Modified residuei169 – 1691N6-succinyllysineBy similarity
Modified residuei316 – 3161N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP13264.
PRIDEiP13264.

PTM databases

PhosphoSiteiP13264.

Expressioni

Tissue specificityi

Kidney, brain, and intestine.

Gene expression databases

GenevestigatoriP13264.

Interactioni

Subunit structurei

Homotetramer. Tetramer composed of 68 and 65 kDa peptides in a 1:3 ratio. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.1 Publication

Protein-protein interaction databases

DIPiDIP-60007N.
IntActiP13264. 1 interaction.
STRINGi10116.ENSRNOP00000063176.

Structurei

3D structure databases

ProteinModelPortaliP13264.
SMRiP13264. Positions 227-536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati590 – 61930ANK 1Add
BLAST
Repeati624 – 65330ANK 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi91 – 999Poly-Gln

Sequence similaritiesi

Belongs to the glutaminase family.Curated
Contains 2 ANK repeats.Curated

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiP13264.
KOiK01425.
PhylomeDBiP13264.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF00023. Ank. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P13264-1) [UniParc]FASTAAdd to Basket

Also known as: KGA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRLRGSAML RELLLRPPAA VGGVLRRTQP LGTLCRRPRG GSRPAAGLVA
60 70 80 90 100
AARLHPWWGG GGRAKGPGSG GLSSSPSEIL QELGKGGTPP QQQQQQQQQP
110 120 130 140 150
GASPPAAPGP KDSPGETDAF GNSEGKEMVA AGDNKVKQGL LPSLEDLLFY
160 170 180 190 200
TIAEGQEKIP VHKFITALKS TGLRTSDPRL KECMDMLRLT LQTTSDGVML
210 220 230 240 250
DKDLFKKCVQ SNIVLLTQAF RRKFVIPDFM SFTSHIDELY ESAKKQSGGK
260 270 280 290 300
VADYIPQLAK FSPDLWGVSV CTVDGQRHSI GDTKVPFCLQ SCVKPLKYAI
310 320 330 340 350
AVNDLGTEYV HRYVGKEPSG LRFNKLFLNE DDKPHNPMVN AGAIVVTSLI
360 370 380 390 400
KQGVNNAEKF DYVMQFLNKM AGNEYVGFSN ATFQSERESG DRNFAIGYYL
410 420 430 440 450
KEKKCFPEGT DMVGILDFYF QLCSIEVTCE SASVMAATLA NGGFCPITGE
460 470 480 490 500
RVLSPEAVRN TLSLMHSCGM YDFSGQFAFH VGLPAKSGVA GGILLVVPNV
510 520 530 540 550
MGMMCWSPPL DKMGNSVKGI HFCHDLVSLC NFHNYDNLRH FAKKLDPRRE
560 570 580 590 600
GGDQRVKSVI NLLFAAYTGD VSALRRFALS AMDMEQRDYD SRTALHVAAA
610 620 630 640 650
EGHVEVVKFL LEACKVNPFP KDRWNNTPMD EALHFGHHDV FKILQEYQVQ
660 670
YTPQGDSDDG KENQTVHKNL DGLL
Length:674
Mass (Da):74,024
Last modified:December 1, 1992 - v2
Checksum:i4B2524A45D3678CA
GO
Isoform 2 (identifier: P13264-2) [UniParc]FASTAAdd to Basket

Also known as: GAC

The sequence of this isoform differs from the canonical sequence as follows:
     556-674: VKSVINLLFA...TVHKNLDGLL → HSFGPLDYES...YRMESLGERS

Show »
Length:603
Mass (Da):65,997
Checksum:iF4DD80AA26873041
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281T → A in AAG30873. (PubMed:11267668)Curated
Sequence conflicti349 – 3513LIK → EFG in AAA41234. (PubMed:3401701)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei556 – 674119VKSVI…LDGLL → HSFGPLDYESLQQELALKDT VWKKVSPESSDDTSTTIVYR MESLGERS in isoform 2. 1 PublicationVSP_041992Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65150 mRNA. Translation: AAA41247.1.
AF302091 Genomic DNA. Translation: AAG30873.1.
AY083459 mRNA. Translation: AAM00020.1.
M22586 mRNA. Translation: AAA41234.1.
PIRiA41009.
RefSeqiNP_001103438.1. NM_001109968.1.
NP_036701.2. NM_012569.2.
UniGeneiRn.5762.

Genome annotation databases

GeneIDi24398.
KEGGirno:24398.
UCSCiRGD:2707. rat. [P13264-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65150 mRNA. Translation: AAA41247.1 .
AF302091 Genomic DNA. Translation: AAG30873.1 .
AY083459 mRNA. Translation: AAM00020.1 .
M22586 mRNA. Translation: AAA41234.1 .
PIRi A41009.
RefSeqi NP_001103438.1. NM_001109968.1.
NP_036701.2. NM_012569.2.
UniGenei Rn.5762.

3D structure databases

ProteinModelPortali P13264.
SMRi P13264. Positions 227-536.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60007N.
IntActi P13264. 1 interaction.
STRINGi 10116.ENSRNOP00000063176.

PTM databases

PhosphoSitei P13264.

Proteomic databases

PaxDbi P13264.
PRIDEi P13264.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24398.
KEGGi rno:24398.
UCSCi RGD:2707. rat. [P13264-1 ]

Organism-specific databases

CTDi 2744.
RGDi 2707. Gls.

Phylogenomic databases

eggNOGi COG2066.
HOGENOMi HOG000216891.
HOVERGENi HBG005856.
InParanoidi P13264.
KOi K01425.
PhylomeDBi P13264.

Enzyme and pathway databases

SABIO-RK P13264.

Miscellaneous databases

NextBioi 603191.
PROi P13264.

Gene expression databases

Genevestigatori P13264.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPi MF_00313. Glutaminase.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view ]
PANTHERi PTHR12544. PTHR12544. 1 hit.
Pfami PF00023. Ank. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view ]
SMARTi SM00248. ANK. 2 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation, characterization, and in vitro expression of a cDNA that encodes the kidney isoenzyme of the mitochondrial glutaminase."
    Shapiro R.A., Farrell L., Srinivasan M., Curthoys N.P.
    J. Biol. Chem. 266:18792-18796(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 73-90.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "Isolation and characterization of the promoter region of the rat kidney-type glutaminase gene."
    Taylor L., Liu X., Newsome W., Shapiro R.A., Srinivasan M., Curthoys N.P.
    Biochim. Biophys. Acta 1518:132-136(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-133.
    Strain: Sprague-Dawley.
  3. "Complexity and species variation of the kidney-type glutaminase gene."
    Porter L.D., Ibrahim H., Taylor L., Curthoys N.P.
    Physiol. Genomics 9:157-166(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 299-674 (ISOFORM 2).
  4. "Isolation of a cDNA for rat brain glutaminase."
    Banner C., Hwang J.-J., Shapiro R.A., Wenthold R.J., Nakatani Y., Lample K.A., Thomas J.W., Huie D., Curthoys N.P.
    Brain Res. 427:247-254(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 349-651 (ISOFORM 1).
    Tissue: Brain.
  5. "Biosynthesis and processing of mitochondrial glutaminase in HTC hepatoma cells."
    Perera S.Y., Voith D.M., Curthoys N.P.
    Biochem. J. 273:265-270(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING.
  6. "Brain-specific BNIP-2-homology protein Caytaxin relocalises glutaminase to neurite terminals and reduces glutamate levels."
    Buschdorf J.P., Li Chew L., Zhang B., Cao Q., Liang F.Y., Liou Y.C., Zhou Y.T., Low B.C.
    J. Cell Sci. 119:3337-3350(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH ATCAY.

Entry informationi

Entry nameiGLSK_RAT
AccessioniPrimary (citable) accession number: P13264
Secondary accession number(s): Q8R421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3