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Protein

Choline-phosphate cytidylyltransferase

Gene

PCT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

CTP + phosphocholine = diphosphate + CDP-choline.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase (PCT1)
  2. Cholinephosphotransferase 1 (CPT1), Choline/ethanolaminephosphotransferase 1 (EPT1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149CTPBy similarity1
Binding sitei149SubstrateBy similarity1
Binding sitei178SubstrateBy similarity1
Binding sitei200CTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi111 – 119CTPBy similarity9
Nucleotide bindingi195 – 196CTPBy similarity2
Nucleotide bindingi223 – 227CTPBy similarity5

GO - Molecular functioni

  • choline-phosphate cytidylyltransferase activity Source: SGD

GO - Biological processi

  • CDP-choline pathway Source: SGD
  • phosphatidylcholine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YGR202C-MONOMER.
YEAST:YGR202C-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.
UniPathwayiUPA00753; UER00739.

Chemistry databases

SwissLipidsiSLP:000000069.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline-phosphate cytidylyltransferase (EC:2.7.7.15)
Alternative name(s):
CTP:phosphocholine cytidylyltransferase
Short name:
CCT
Short name:
CT
Phosphorylcholine transferase
Gene namesi
Name:PCT1
Synonyms:CCT, CCT1
Ordered Locus Names:YGR202C
ORF Names:G7729
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR202C.
SGDiS000003434. PCT1.

Subcellular locationi

  • Membrane

  • Note: Most of its activity is membrane-associated.

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • membrane Source: UniProtKB-SubCell
  • nuclear envelope Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002084601 – 424Choline-phosphate cytidylyltransferaseAdd BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineCombined sources1
Modified residuei59PhosphothreonineCombined sources1
Modified residuei346PhosphoserineCombined sources1
Modified residuei401Phosphoserine; by CK2Sequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13259.
PRIDEiP13259.

PTM databases

iPTMnetiP13259.

Interactioni

Protein-protein interaction databases

BioGridi33455. 53 interactors.
DIPiDIP-6390N.
IntActiP13259. 15 interactors.
MINTiMINT-2785040.

Structurei

3D structure databases

ProteinModelPortaliP13259.
SMRiP13259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytidylyltransferase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000269.
HOGENOMiHOG000208588.
InParanoidiP13259.
KOiK00968.
OMAiLNNASRD.
OrthoDBiEOG092C3LI1.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

P13259-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPTTGKSS IRAKLSNSSL SNLFKKNKNK RQREETEEQD NEDKDESKNQ
60 70 80 90 100
DENKDTQLTP RKRRRLTKEF EEKEARYTNE LPKELRKYRP KGFRFNLPPT
110 120 130 140 150
DRPIRIYADG VFDLFHLGHM KQLEQCKKAF PNVTLIVGVP SDKITHKLKG
160 170 180 190 200
LTVLTDKQRC ETLTHCRWVD EVVPNAPWCV TPEFLLEHKI DYVAHDDIPY
210 220 230 240 250
VSADSDDIYK PIKEMGKFLT TQRTNGVSTS DIITKIIRDY DKYLMRNFAR
260 270 280 290 300
GATRQELNVS WLKKNELEFK KHINEFRSYF KKNQTNLNNA SRDLYFEVRE
310 320 330 340 350
ILLKKTLGKK LYSKLIGNEL KKQNQRQRKQ NFLDDPFTRK LIREASPATE
360 370 380 390 400
FANEFTGENS TAKSPDDNGN LFSQEDDEDT NSNNTNTNSD SDSNTNSTPP
410 420
SEDDDDNDRL TLENLTQKKK QSAN
Length:424
Mass (Da):49,406
Last modified:September 27, 2004 - v2
Checksum:i246774FE2F7124A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90P → PG in AAA91962 (PubMed:2826147).Curated1
Sequence conflicti105Missing in AAA91962 (PubMed:2826147).Curated1
Sequence conflicti192Y → H in AAA91962 (PubMed:2826147).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36827 Genomic DNA. Translation: AAA91962.1.
Z49133 Genomic DNA. Translation: CAA88995.1.
Z72987 Genomic DNA. Translation: CAA97229.1.
AY557840 Genomic DNA. Translation: AAS56166.1.
BK006941 Genomic DNA. Translation: DAA08295.1.
PIRiS53925. XNBYCP.
RefSeqiNP_011718.1. NM_001181331.1.

Genome annotation databases

EnsemblFungiiYGR202C; YGR202C; YGR202C.
GeneIDi853116.
KEGGisce:YGR202C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36827 Genomic DNA. Translation: AAA91962.1.
Z49133 Genomic DNA. Translation: CAA88995.1.
Z72987 Genomic DNA. Translation: CAA97229.1.
AY557840 Genomic DNA. Translation: AAS56166.1.
BK006941 Genomic DNA. Translation: DAA08295.1.
PIRiS53925. XNBYCP.
RefSeqiNP_011718.1. NM_001181331.1.

3D structure databases

ProteinModelPortaliP13259.
SMRiP13259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33455. 53 interactors.
DIPiDIP-6390N.
IntActiP13259. 15 interactors.
MINTiMINT-2785040.

Chemistry databases

SwissLipidsiSLP:000000069.

PTM databases

iPTMnetiP13259.

Proteomic databases

MaxQBiP13259.
PRIDEiP13259.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR202C; YGR202C; YGR202C.
GeneIDi853116.
KEGGisce:YGR202C.

Organism-specific databases

EuPathDBiFungiDB:YGR202C.
SGDiS000003434. PCT1.

Phylogenomic databases

GeneTreeiENSGT00390000000269.
HOGENOMiHOG000208588.
InParanoidiP13259.
KOiK00968.
OMAiLNNASRD.
OrthoDBiEOG092C3LI1.

Enzyme and pathway databases

UniPathwayiUPA00753; UER00739.
BioCyciMetaCyc:YGR202C-MONOMER.
YEAST:YGR202C-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.

Miscellaneous databases

PROiP13259.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCY1_YEAST
AccessioniPrimary (citable) accession number: P13259
Secondary accession number(s): D6VUY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: September 27, 2004
Last modified: November 30, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3050 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.