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Protein

Choline-phosphate cytidylyltransferase

Gene

PCT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

CTP + phosphocholine = diphosphate + CDP-choline.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase (PCT1)
  2. Cholinephosphotransferase 1 (CPT1), Choline/ethanolaminephosphotransferase 1 (EPT1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491CTPBy similarity
Binding sitei149 – 1491SubstrateBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Binding sitei200 – 2001CTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi111 – 1199CTPBy similarity
Nucleotide bindingi195 – 1962CTPBy similarity
Nucleotide bindingi223 – 2275CTPBy similarity

GO - Molecular functioni

  • choline-phosphate cytidylyltransferase activity Source: SGD

GO - Biological processi

  • CDP-choline pathway Source: SGD
  • phosphatidylcholine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13670.
YEAST:YGR202C-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.
UniPathwayiUPA00753; UER00739.

Chemistry

SwissLipidsiSLP:000000069.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline-phosphate cytidylyltransferase (EC:2.7.7.15)
Alternative name(s):
CTP:phosphocholine cytidylyltransferase
Short name:
CCT
Short name:
CT
Phosphorylcholine transferase
Gene namesi
Name:PCT1
Synonyms:CCT, CCT1
Ordered Locus Names:YGR202C
ORF Names:G7729
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR202C.
SGDiS000003434. PCT1.

Subcellular locationi

  • Membrane

  • Note: Most of its activity is membrane-associated.

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • membrane Source: UniProtKB-SubCell
  • nuclear envelope Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Choline-phosphate cytidylyltransferasePRO_0000208460Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161PhosphoserineCombined sources
Modified residuei59 – 591PhosphothreonineCombined sources
Modified residuei346 – 3461PhosphoserineCombined sources
Modified residuei401 – 4011Phosphoserine; by CK2Sequence analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13259.
PeptideAtlasiP13259.

PTM databases

iPTMnetiP13259.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP1P206763EBI-5254,EBI-12392
SRP1Q028214EBI-5254,EBI-1797

Protein-protein interaction databases

BioGridi33455. 53 interactions.
DIPiDIP-6390N.
IntActiP13259. 15 interactions.
MINTiMINT-2785040.

Structurei

3D structure databases

ProteinModelPortaliP13259.
SMRiP13259. Positions 99-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytidylyltransferase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000269.
HOGENOMiHOG000208588.
InParanoidiP13259.
KOiK00968.
OMAiLNNASRD.
OrthoDBiEOG7NSBD8.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

P13259-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPTTGKSS IRAKLSNSSL SNLFKKNKNK RQREETEEQD NEDKDESKNQ
60 70 80 90 100
DENKDTQLTP RKRRRLTKEF EEKEARYTNE LPKELRKYRP KGFRFNLPPT
110 120 130 140 150
DRPIRIYADG VFDLFHLGHM KQLEQCKKAF PNVTLIVGVP SDKITHKLKG
160 170 180 190 200
LTVLTDKQRC ETLTHCRWVD EVVPNAPWCV TPEFLLEHKI DYVAHDDIPY
210 220 230 240 250
VSADSDDIYK PIKEMGKFLT TQRTNGVSTS DIITKIIRDY DKYLMRNFAR
260 270 280 290 300
GATRQELNVS WLKKNELEFK KHINEFRSYF KKNQTNLNNA SRDLYFEVRE
310 320 330 340 350
ILLKKTLGKK LYSKLIGNEL KKQNQRQRKQ NFLDDPFTRK LIREASPATE
360 370 380 390 400
FANEFTGENS TAKSPDDNGN LFSQEDDEDT NSNNTNTNSD SDSNTNSTPP
410 420
SEDDDDNDRL TLENLTQKKK QSAN
Length:424
Mass (Da):49,406
Last modified:September 27, 2004 - v2
Checksum:i246774FE2F7124A3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901P → PG in AAA91962 (PubMed:2826147).Curated
Sequence conflicti105 – 1051Missing in AAA91962 (PubMed:2826147).Curated
Sequence conflicti192 – 1921Y → H in AAA91962 (PubMed:2826147).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36827 Genomic DNA. Translation: AAA91962.1.
Z49133 Genomic DNA. Translation: CAA88995.1.
Z72987 Genomic DNA. Translation: CAA97229.1.
AY557840 Genomic DNA. Translation: AAS56166.1.
BK006941 Genomic DNA. Translation: DAA08295.1.
PIRiS53925. XNBYCP.
RefSeqiNP_011718.1. NM_001181331.1.

Genome annotation databases

EnsemblFungiiYGR202C; YGR202C; YGR202C.
GeneIDi853116.
KEGGisce:YGR202C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36827 Genomic DNA. Translation: AAA91962.1.
Z49133 Genomic DNA. Translation: CAA88995.1.
Z72987 Genomic DNA. Translation: CAA97229.1.
AY557840 Genomic DNA. Translation: AAS56166.1.
BK006941 Genomic DNA. Translation: DAA08295.1.
PIRiS53925. XNBYCP.
RefSeqiNP_011718.1. NM_001181331.1.

3D structure databases

ProteinModelPortaliP13259.
SMRiP13259. Positions 99-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33455. 53 interactions.
DIPiDIP-6390N.
IntActiP13259. 15 interactions.
MINTiMINT-2785040.

Chemistry

SwissLipidsiSLP:000000069.

PTM databases

iPTMnetiP13259.

Proteomic databases

MaxQBiP13259.
PeptideAtlasiP13259.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR202C; YGR202C; YGR202C.
GeneIDi853116.
KEGGisce:YGR202C.

Organism-specific databases

EuPathDBiFungiDB:YGR202C.
SGDiS000003434. PCT1.

Phylogenomic databases

GeneTreeiENSGT00390000000269.
HOGENOMiHOG000208588.
InParanoidiP13259.
KOiK00968.
OMAiLNNASRD.
OrthoDBiEOG7NSBD8.

Enzyme and pathway databases

UniPathwayiUPA00753; UER00739.
BioCyciMetaCyc:MONOMER-13670.
YEAST:YGR202C-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.

Miscellaneous databases

PROiP13259.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae."
    Tsukagoshi Y., Nikawa J., Yamashita S.
    Eur. J. Biochem. 169:477-486(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs."
    Guerreiro P., Barreiros T., Soares H., Cyrne L., Maia e Silva A., Rodrigues-Pousada C.
    Yeast 12:273-280(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-346, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; THR-59 AND SER-346, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-346, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPCY1_YEAST
AccessioniPrimary (citable) accession number: P13259
Secondary accession number(s): D6VUY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: September 27, 2004
Last modified: June 8, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3050 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.