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Protein

Choline-phosphate cytidylyltransferase

Gene

PCT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 3050 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

CTP + phosphocholine = diphosphate + CDP-choline.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase (PCT1)
  2. Cholinephosphotransferase 1 (CPT1), Choline/ethanolaminephosphotransferase 1 (EPT1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149CTPBy similarity1
Binding sitei149SubstrateBy similarity1
Binding sitei178SubstrateBy similarity1
Binding sitei200CTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi111 – 119CTPBy similarity9
Nucleotide bindingi195 – 196CTPBy similarity2
Nucleotide bindingi223 – 227CTPBy similarity5

GO - Molecular functioni

  • choline-phosphate cytidylyltransferase activity Source: SGD

GO - Biological processi

  • CDP-choline pathway Source: SGD
  • phosphatidylcholine biosynthetic process Source: SGD

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YGR202C-MONOMER
YEAST:YGR202C-MONOMER
ReactomeiR-SCE-1483191 Synthesis of PC
UniPathwayiUPA00753; UER00739

Chemistry databases

SwissLipidsiSLP:000000069

Names & Taxonomyi

Protein namesi
Recommended name:
Choline-phosphate cytidylyltransferase (EC:2.7.7.15)
Alternative name(s):
CTP:phosphocholine cytidylyltransferase
Short name:
CCT
Short name:
CT
Phosphorylcholine transferase
Gene namesi
Name:PCT1
Synonyms:CCT, CCT1
Ordered Locus Names:YGR202C
ORF Names:G7729
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR202C
SGDiS000003434 PCT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002084601 – 424Choline-phosphate cytidylyltransferaseAdd BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineCombined sources1
Modified residuei59PhosphothreonineCombined sources1
Modified residuei346PhosphoserineCombined sources1
Modified residuei401Phosphoserine; by CK2Sequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13259
PaxDbiP13259
PRIDEiP13259

PTM databases

iPTMnetiP13259

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33455, 105 interactors
DIPiDIP-6390N
IntActiP13259, 19 interactors
MINTiP13259
STRINGi4932.YGR202C

Structurei

3D structure databases

ProteinModelPortaliP13259
SMRiP13259
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytidylyltransferase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000269
HOGENOMiHOG000208588
InParanoidiP13259
KOiK00968
OMAiPNVELVC
OrthoDBiEOG092C3LI1

Family and domain databases

Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR004821 Cyt_trans-like
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01467 CTP_transf_like, 1 hit
TIGRFAMsiTIGR00125 cyt_tran_rel, 1 hit

Sequencei

Sequence statusi: Complete.

P13259-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPTTGKSS IRAKLSNSSL SNLFKKNKNK RQREETEEQD NEDKDESKNQ
60 70 80 90 100
DENKDTQLTP RKRRRLTKEF EEKEARYTNE LPKELRKYRP KGFRFNLPPT
110 120 130 140 150
DRPIRIYADG VFDLFHLGHM KQLEQCKKAF PNVTLIVGVP SDKITHKLKG
160 170 180 190 200
LTVLTDKQRC ETLTHCRWVD EVVPNAPWCV TPEFLLEHKI DYVAHDDIPY
210 220 230 240 250
VSADSDDIYK PIKEMGKFLT TQRTNGVSTS DIITKIIRDY DKYLMRNFAR
260 270 280 290 300
GATRQELNVS WLKKNELEFK KHINEFRSYF KKNQTNLNNA SRDLYFEVRE
310 320 330 340 350
ILLKKTLGKK LYSKLIGNEL KKQNQRQRKQ NFLDDPFTRK LIREASPATE
360 370 380 390 400
FANEFTGENS TAKSPDDNGN LFSQEDDEDT NSNNTNTNSD SDSNTNSTPP
410 420
SEDDDDNDRL TLENLTQKKK QSAN
Length:424
Mass (Da):49,406
Last modified:September 27, 2004 - v2
Checksum:i246774FE2F7124A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90P → PG in AAA91962 (PubMed:2826147).Curated1
Sequence conflicti105Missing in AAA91962 (PubMed:2826147).Curated1
Sequence conflicti192Y → H in AAA91962 (PubMed:2826147).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36827 Genomic DNA Translation: AAA91962.1
Z49133 Genomic DNA Translation: CAA88995.1
Z72987 Genomic DNA Translation: CAA97229.1
AY557840 Genomic DNA Translation: AAS56166.1
BK006941 Genomic DNA Translation: DAA08295.1
PIRiS53925 XNBYCP
RefSeqiNP_011718.1, NM_001181331.1

Genome annotation databases

EnsemblFungiiYGR202C; YGR202C; YGR202C
GeneIDi853116
KEGGisce:YGR202C

Similar proteinsi

Entry informationi

Entry nameiPCY1_YEAST
AccessioniPrimary (citable) accession number: P13259
Secondary accession number(s): D6VUY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: September 27, 2004
Last modified: March 28, 2018
This is version 179 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health