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Protein

Argininosuccinate synthase

Gene

argG

Organism
Methanosarcina barkeri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861CitrullineUniRule annotation
Binding sitei91 – 911CitrullineUniRule annotation
Binding sitei116 – 1161ATP; via amide nitrogenUniRule annotation
Binding sitei118 – 1181AspartateUniRule annotation
Binding sitei122 – 1221AspartateUniRule annotation
Binding sitei122 – 1221CitrullineUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei126 – 1261CitrullineUniRule annotation
Binding sitei172 – 1721CitrullineUniRule annotation
Binding sitei181 – 1811CitrullineUniRule annotation
Binding sitei257 – 2571CitrullineUniRule annotation
Binding sitei269 – 2691CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPUniRule annotation

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
OrganismiMethanosarcina barkeri
Taxonomic identifieri2208 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Argininosuccinate synthasePRO_0000148675Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP13257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKVALAYS GGLDTSVCIP ILKEKYGYDE VVTISVDVGQ PEEEIKRADA
60 70 80 90 100
KAEKISNKHY TIDAKEEFVK DYVFPLIKAN GSYEGYVMGT SIARPLIAKK
110 120 130 140 150
VVEAARKEGA VALAHGCTGK GNDQLRFEAV FRQTDMDVIA PMREMNLTRE
160 170 180 190 200
WEIDYAKEHG IPVEVTKSKP WSVDENIWSR SIEGGRLEDP SFVPPEEIFE
210 220 230 240 250
WTTSPEKAPE QPRVVDIGFE AGVPVSLDGE KMSGYAFVKK MNEIAGENGV
260 270 280 290 300
GRTDMIEDRV LGLKARENYE HPAATVLRQP NSRPLRNSSL TRSELKFKKI
310 320 330 340 350
VDDQWSELAY YGLVDEPLYA DLNAFIDKSQ ERVTGIVKVR LYKGALTILA
360 370 380 390
RSSPNALYSE DLVSFDSQTI DQKGSEGFAK YHGFQARMYR KVMDKQ
Length:396
Mass (Da):44,441
Last modified:December 31, 1989 - v1
Checksum:iBF9DA4207D1ACC6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21314 Genomic DNA. Translation: AAA72677.1.
PIRiB28180. AJMZRB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21314 Genomic DNA. Translation: AAA72677.1.
PIRiB28180. AJMZRB.

3D structure databases

ProteinModelPortaliP13257.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Conservation of structure in the human gene encoding argininosuccinate synthetase and the argG genes of the archaebacteria Methanosarcina barkeri MS and Methanococcus vannielii."
    Morris C.J., Reeve J.N.
    J. Bacteriol. 170:3125-3130(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43569 / MS / DSM 800 / JCM 10043 / NBRC 100474.

Entry informationi

Entry nameiASSY_METBA
AccessioniPrimary (citable) accession number: P13257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 31, 1989
Last sequence update: December 31, 1989
Last modified: January 6, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.