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P13244 (MASY_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase, glyoxysomal

EC=2.3.3.9
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 561561Malate synthase, glyoxysomal
PRO_0000166866

Regions

Motif559 – 5613Microbody targeting signal Potential

Sites

Active site1771Proton acceptor By similarity
Active site4621Proton donor By similarity

Experimental info

Sequence conflict291Q → L in CAA73793. Ref.2
Sequence conflict1631K → I in CAA73793. Ref.2
Sequence conflict1721A → V in CAA73793. Ref.2
Sequence conflict1751F → W in CAA73793. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P13244 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 215A1027CD991AA3

FASTA56163,728
        10         20         30         40         50         60 
MELETSVYRP NVAVYDSPDG VEVRGRYDQV FAKILTRDAL GFVAELQREF RGHVRYAMEC 

        70         80         90        100        110        120 
RREVKRRYNS GAVPGFDPST KFIRDGEWVC ASVPPAVADR RVEITGPVER KMIINALNSG 

       130        140        150        160        170        180 
AKVFMADFED ALSPSWENLM RGQVNLKDAV DGSITFNDKA RNKVYKLNDQ VAKLFVRPRG 

       190        200        210        220        230        240 
WHLPEAHILI DGEPATGCLV DFGLYFFHNY AKFRQTQGSG FGPFFYLPKM EHSREAKIWN 

       250        260        270        280        290        300 
SVFERAEKMA GIERGSIRAT VLIETLPAVF QMNEILYELR DHSVGLNCGR WDYIFSYVKT 

       310        320        330        340        350        360 
FQAHPDRLLP DRVLVGMGQH FMRSYSDLLI RTCHKRGVHA MGGMAAQIPI RDDPKANEMA 

       370        380        390        400        410        420 
LDLVKKDKLR EVRAGHDGTW AAHPGLIPIC MDAFSHMGNN PNQIKSMKRD DASAITEEDL 

       430        440        450        460        470        480 
LQIPRGVRTL EGLRLNTRVG IQYLAAWLTG SGSVPLYNLM EDAATAEISR VQNWQWIRYG 

       490        500        510        520        530        540 
VELDGDGLGV RVSKELFGRV VEEEMERIEK EVGKDKFKRG MYKEACKMFT KQCTAAELDD 

       550        560 
FLTLAVYDHI VAHYPINASR L 

« Hide

References

[1]"Deduced sequence of a malate synthase polypeptide encoded by a subclass of the gene family."
Comai L., Baden C.S., Harada J.J.
J. Biol. Chem. 264:2778-2782(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Olesen C., Thomsen K.K., Svendsen I., Brandt A.B.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Global.
Tissue: Cotyledon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04468 mRNA. Translation: AAA32996.1.
Y13357 mRNA. Translation: CAA73793.1.
PIRSYRPMA. A31428.

3D structure databases

ProteinModelPortalP13244.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP13244.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMASY_BRANA
AccessionPrimary (citable) accession number: P13244
Secondary accession number(s): O04911
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways