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Protein

Malate synthase, glyoxysomal

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Proton acceptorBy similarity
Active sitei462 – 4621Proton donorBy similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase, glyoxysomal (EC:2.3.3.9)
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Malate synthase, glyoxysomalPRO_0000166866Add
BLAST

Proteomic databases

PRIDEiP13244.

Structurei

3D structure databases

ProteinModelPortaliP13244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi559 – 5613Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELETSVYRP NVAVYDSPDG VEVRGRYDQV FAKILTRDAL GFVAELQREF
60 70 80 90 100
RGHVRYAMEC RREVKRRYNS GAVPGFDPST KFIRDGEWVC ASVPPAVADR
110 120 130 140 150
RVEITGPVER KMIINALNSG AKVFMADFED ALSPSWENLM RGQVNLKDAV
160 170 180 190 200
DGSITFNDKA RNKVYKLNDQ VAKLFVRPRG WHLPEAHILI DGEPATGCLV
210 220 230 240 250
DFGLYFFHNY AKFRQTQGSG FGPFFYLPKM EHSREAKIWN SVFERAEKMA
260 270 280 290 300
GIERGSIRAT VLIETLPAVF QMNEILYELR DHSVGLNCGR WDYIFSYVKT
310 320 330 340 350
FQAHPDRLLP DRVLVGMGQH FMRSYSDLLI RTCHKRGVHA MGGMAAQIPI
360 370 380 390 400
RDDPKANEMA LDLVKKDKLR EVRAGHDGTW AAHPGLIPIC MDAFSHMGNN
410 420 430 440 450
PNQIKSMKRD DASAITEEDL LQIPRGVRTL EGLRLNTRVG IQYLAAWLTG
460 470 480 490 500
SGSVPLYNLM EDAATAEISR VQNWQWIRYG VELDGDGLGV RVSKELFGRV
510 520 530 540 550
VEEEMERIEK EVGKDKFKRG MYKEACKMFT KQCTAAELDD FLTLAVYDHI
560
VAHYPINASR L
Length:561
Mass (Da):63,728
Last modified:January 1, 1990 - v1
Checksum:i215A1027CD991AA3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291Q → L in CAA73793 (Ref. 2) Curated
Sequence conflicti163 – 1631K → I in CAA73793 (Ref. 2) Curated
Sequence conflicti172 – 1721A → V in CAA73793 (Ref. 2) Curated
Sequence conflicti175 – 1751F → W in CAA73793 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04468 mRNA. Translation: AAA32996.1.
Y13357 mRNA. Translation: CAA73793.1.
PIRiA31428. SYRPMA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04468 mRNA. Translation: AAA32996.1.
Y13357 mRNA. Translation: CAA73793.1.
PIRiA31428. SYRPMA.

3D structure databases

ProteinModelPortaliP13244.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP13244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Deduced sequence of a malate synthase polypeptide encoded by a subclass of the gene family."
    Comai L., Baden C.S., Harada J.J.
    J. Biol. Chem. 264:2778-2782(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Olesen C., Thomsen K.K., Svendsen I., Brandt A.B.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Global.
    Tissue: Cotyledon.

Entry informationi

Entry nameiMASY_BRANA
AccessioniPrimary (citable) accession number: P13244
Secondary accession number(s): O04911
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.