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Protein

Malate synthase, glyoxysomal

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase
  2. Malate synthase (BnaC02g02870D), Malate synthase (BnaAnng00830D), Malate synthase, glyoxysomal
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei177Proton acceptorBy similarity1
Active sitei462Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase, glyoxysomal (EC:2.3.3.9)
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001668661 – 561Malate synthase, glyoxysomalAdd BLAST561

Proteomic databases

PRIDEiP13244.

Structurei

3D structure databases

ProteinModelPortaliP13244.
SMRiP13244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi559 – 561Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

KOiK01638.
OMAiETAHIRA.

Family and domain databases

CDDicd00727. malate_synt_A. 1 hit.
InterProiView protein in InterPro
IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
PANTHERiPTHR42902. PTHR42902. 1 hit.
PfamiView protein in Pfam
PF01274. Malate_synthase. 1 hit.
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiView protein in PROSITE
PS00510. MALATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P13244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELETSVYRP NVAVYDSPDG VEVRGRYDQV FAKILTRDAL GFVAELQREF
60 70 80 90 100
RGHVRYAMEC RREVKRRYNS GAVPGFDPST KFIRDGEWVC ASVPPAVADR
110 120 130 140 150
RVEITGPVER KMIINALNSG AKVFMADFED ALSPSWENLM RGQVNLKDAV
160 170 180 190 200
DGSITFNDKA RNKVYKLNDQ VAKLFVRPRG WHLPEAHILI DGEPATGCLV
210 220 230 240 250
DFGLYFFHNY AKFRQTQGSG FGPFFYLPKM EHSREAKIWN SVFERAEKMA
260 270 280 290 300
GIERGSIRAT VLIETLPAVF QMNEILYELR DHSVGLNCGR WDYIFSYVKT
310 320 330 340 350
FQAHPDRLLP DRVLVGMGQH FMRSYSDLLI RTCHKRGVHA MGGMAAQIPI
360 370 380 390 400
RDDPKANEMA LDLVKKDKLR EVRAGHDGTW AAHPGLIPIC MDAFSHMGNN
410 420 430 440 450
PNQIKSMKRD DASAITEEDL LQIPRGVRTL EGLRLNTRVG IQYLAAWLTG
460 470 480 490 500
SGSVPLYNLM EDAATAEISR VQNWQWIRYG VELDGDGLGV RVSKELFGRV
510 520 530 540 550
VEEEMERIEK EVGKDKFKRG MYKEACKMFT KQCTAAELDD FLTLAVYDHI
560
VAHYPINASR L
Length:561
Mass (Da):63,728
Last modified:January 1, 1990 - v1
Checksum:i215A1027CD991AA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29Q → L in CAA73793 (Ref. 2) Curated1
Sequence conflicti163K → I in CAA73793 (Ref. 2) Curated1
Sequence conflicti172A → V in CAA73793 (Ref. 2) Curated1
Sequence conflicti175F → W in CAA73793 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04468 mRNA. Translation: AAA32996.1.
Y13357 mRNA. Translation: CAA73793.1.
PIRiA31428. SYRPMA.
RefSeqiXP_013720273.1. XM_013864819.1.
XP_013721514.1. XM_013866060.1.

Genome annotation databases

EnsemblPlantsiCDY04901; CDY04901; GSBRNA2T00119622001.
GeneIDi106424075.
106425327.
GrameneiCDY04901; CDY04901; GSBRNA2T00119622001.
KEGGibna:106424075.
bna:106425327.

Similar proteinsi

Entry informationi

Entry nameiMASY_BRANA
AccessioniPrimary (citable) accession number: P13244
Secondary accession number(s): O04911
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: August 30, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families