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Protein

Probable fructose-bisphosphate aldolase

Gene

fbaA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. Probable fructose-bisphosphate aldolase (fbaA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50Glyceraldehyde 3-phosphateBy similarity1
Active sitei85Proton donorBy similarity1
Metal bindingi86Zinc 1; catalyticBy similarity1
Metal bindingi107Zinc 2By similarity1
Metal bindingi137Zinc 2By similarity1
Metal bindingi181Zinc 1; catalyticBy similarity1
Binding sitei182Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi209Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis, Sporulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU37120-MONOMER.
SABIO-RKP13243.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fbaA
Synonyms:fba, fba1, tsr
Ordered Locus Names:BSU37120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787051 – 285Probable fructose-bisphosphate aldolaseAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei212Phosphothreonine1 Publication1
Modified residuei234Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated during sporulation.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13243.
PRIDEiP13243.

PTM databases

iPTMnetiP13243.

Interactioni

Protein-protein interaction databases

IntActiP13243. 1 interactor.
MINTiMINT-8365567.
STRINGi224308.Bsubs1_010100020066.

Structurei

3D structure databases

ProteinModelPortaliP13243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 212Dihydroxyacetone phosphate bindingBy similarity3
Regioni231 – 234Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D2N. Bacteria.
COG0191. LUCA.
HOGENOMiHOG000227793.
InParanoidiP13243.
KOiK01624.
OMAiVNTREMF.
PhylomeDBiP13243.

Family and domain databases

CDDicd00947. TBP_aldolase_IIB. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR011289. Fruc_bis_ald_class-2.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLVSMTEML NTAKEKGYAV GQFNLNNLEF TQAILQAAEE EKSPVILGVS
60 70 80 90 100
EGAGRYMGGF KTVVAMVKAL MEEYKVTVPV AIHLDHGSSF ESCAKAIHAG
110 120 130 140 150
FTSVMIDASH HPFEENVATT AKVVELAHFH GVSVEAELGT VGGQEDDVIA
160 170 180 190 200
EGVIYADPKE CQELVERTGI DCLAPALGSV HGPYKGEPNL GFKEMEEIGK
210 220 230 240 250
STGLPLVLHG GTGIPTADIK KSISLGTAKI NVNTENQISS AKAVRETLAA
260 270 280
KPDEYDPRKY LGPAREAIKE TVIGKMREFG SSNQA
Length:285
Mass (Da):30,401
Last modified:June 1, 1994 - v2
Checksum:i482D3CE048583BCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22039 Unassigned DNA. Translation: AAA16803.1.
S42590 Genomic DNA. Translation: AAB22716.1.
Z49782 Genomic DNA. Translation: CAA89873.1.
AL009126 Genomic DNA. Translation: CAB15729.1.
PIRiS55426. D32354.
RefSeqiNP_391593.1. NC_000964.3.
WP_003243339.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15729; CAB15729; BSU37120.
GeneIDi937040.
KEGGibsu:BSU37120.
PATRICi18979460. VBIBacSub10457_3892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22039 Unassigned DNA. Translation: AAA16803.1.
S42590 Genomic DNA. Translation: AAB22716.1.
Z49782 Genomic DNA. Translation: CAA89873.1.
AL009126 Genomic DNA. Translation: CAB15729.1.
PIRiS55426. D32354.
RefSeqiNP_391593.1. NC_000964.3.
WP_003243339.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP13243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13243. 1 interactor.
MINTiMINT-8365567.
STRINGi224308.Bsubs1_010100020066.

PTM databases

iPTMnetiP13243.

Proteomic databases

PaxDbiP13243.
PRIDEiP13243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15729; CAB15729; BSU37120.
GeneIDi937040.
KEGGibsu:BSU37120.
PATRICi18979460. VBIBacSub10457_3892.

Phylogenomic databases

eggNOGiENOG4105D2N. Bacteria.
COG0191. LUCA.
HOGENOMiHOG000227793.
InParanoidiP13243.
KOiK01624.
OMAiVNTREMF.
PhylomeDBiP13243.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciBSUB:BSU37120-MONOMER.
SABIO-RKP13243.

Family and domain databases

CDDicd00947. TBP_aldolase_IIB. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR011289. Fruc_bis_ald_class-2.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_BACSU
AccessioniPrimary (citable) accession number: P13243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.