P13232 (IL7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-7
Short name=IL-7

Gene names

Name:

IL7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
Subcellular location	Secreted.
Sequence similarities	Belongs to the IL-7/IL-9 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell lineage commitment Inferred from electronic annotation. Source: Compara bone resorption Inferred from sequence or structural similarity. Source: UniProtKB cell-cell signaling Traceable author statement Ref.1. Source: ProtInc homeostasis of number of cells within a tissue Inferred from electronic annotation. Source: Compara humoral immune response Traceable author statement Ref.1. Source: ProtInc negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of catalytic activity Inferred from electronic annotation. Source: Compara organ morphogenesis Traceable author statement Ref.1. Source: ProtInc positive regulation of B cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of T cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of organ growth Inferred from electronic annotation. Source: Compara regulation of gene expression Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	growth factor activity Inferred from sequence or structural similarity. Source: UniProtKB interleukin-7 receptor binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IL2RG	P31785	2	EBI-80516,EBI-80475
IL7R	P16871	3	EBI-80516,EBI-80490

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P13232-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P13232-2)

The sequence of this isoform differs from the canonical sequence as follows:
     77-120: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Chain

26 – 177

152

Interleukin-7

PRO_0000015623

Amino acid modifications

Glycosylation

95

1

N-linked (GlcNAc...) Potential

Glycosylation

116

1

N-linked (GlcNAc...) Potential

Glycosylation

141

1

N-linked (GlcNAc...) Potential

Disulfide bond

27 ↔ 166

Ref.8 Ref.11

Disulfide bond

59 ↔ 154

Ref.8 Ref.11

Disulfide bond

72 ↔ 117

Ref.8 Ref.11

Natural variations

Alternative sequence

77 – 120

44

Missing in isoform 2.

VSP_042926

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

177

Helix Strand Turn

Details...

Helix

34 – 39

6

Helix

41 – 50

10

Beta strand

53 – 55

3

Turn

57 – 59

3

Helix

65 – 69

5

Helix

77 – 89

13

Helix

99 – 115

17

Helix

153 – 171

19

Turn

172 – 176

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 8FC5243F9169617F	FASTA	177	20,187
	10 20 30 40 50 60 MFHVSFRYIF GLPPLILVLL PVASSDCDIE GKDGKQYESV LMVSIDQLLD SMKEIGSNCL 70 80 90 100 110 120 NNEFNFFKRH ICDANKEGMF LFRAARKLRQ FLKMNSTGDF DLHLLKVSEG TTILLNCTGQ 130 140 150 160 170 VKGRKPAALG EAQPTKSLEE NKSLKEQKKL NDLCFLKRLL QEIKTCWNKI LMGTKEH
	Isoform 2 [UniParc]. Checksum: B2DD9AB838924186	FASTA	133	15,203
	10 20 30 40 50 60 MFHVSFRYIF GLPPLILVLL PVASSDCDIE GKDGKQYESV LMVSIDQLLD SMKEIGSNCL 70 80 90 100 110 120 NNEFNFFKRH ICDANKVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK 130 TCWNKILMGT KEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells." Goodwin R.G., Lupton S., Schmierer A., Hjerrild K.J., Jerzy R., Clevenger W., Gillis S., Cosman D., Namen A.E. Proc. Natl. Acad. Sci. U.S.A. 86:302-306(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Characterization of the human and murine IL-7 genes." Lupton S.D., Gimpel S., Jerzy R., Brunton L.L., Hjerrild K.A., Cosman D., Goodwin R.G. J. Immunol. 144:3592-3601(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"interleukin7 mRNA,nirs splice variant2." Sameshima E., Tabata Y., Hayashi A., Iida K., Mitsuyama M., Kanai S., Furuya T. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	Livingston R.J., Shaffer T., McFarland I., Nguyen C.P., Stanaway I.B., Rajkumar N., Johnson E.J., da Ponte S.H., Willa H., Ahearn M.O., Bertucci C., Acklestad J., Carroll A., Swanson J., Gildersleeve H.I., Nickerson D.A. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreas.
[8]	"Disulfide bond assignment in human interleukin-7 by matrix-assisted laser desorption/ionization mass spectroscopy and site-directed cysteine to serine mutational analysis." Cosenza L., Sweeney E., Murphy J.R. J. Biol. Chem. 272:32995-33000(1997) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS, MASS SPECTROMETRY.
[9]	"Prediction of the three-dimensional structure of human interleukin-7 by homology modeling." Kroemer R.T., Doughty S.W., Robinson A.J., Richards W.G. Protein Eng. 9:493-498(1996) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[10]	"Comparative model building of interleukin-7 using interleukin-4 as a template: a structural hypothesis that displays atypical surface chemistry in helix D important for receptor activation." Cosenza L., Rosenbach A., White J.V., Murphy J.R., Smith T.F. Protein Sci. 9:916-926(2000) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[11]	"Structural and biophysical studies of the human IL-7/IL-7Ralpha complex." McElroy C.A., Dohm J.A., Walsh S.T. Structure 17:54-65(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 26-177 IN COMPLEX WITH IL7R, DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

Wikipedia

Interleukin-7 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J04156 mRNA. Translation: AAA59156.1.
M29053 , M29048, M29049, M29050, M29051, M29052 Genomic DNA. Translation: AAC63047.1.
AB102879 mRNA. Translation: BAD89408.1.
EF064721 Genomic DNA. Translation: ABK41904.1.
AC083837 Genomic DNA. No translation available.
AC100854 Genomic DNA. No translation available.
CH471068 Genomic DNA. Translation: EAW87060.1.
CH471068 Genomic DNA. Translation: EAW87062.1.
BC047698 mRNA. Translation: AAH47698.1.

IPI

IPI00007748.
IPI00744116.

PIR

A32223. A43527.
B32223.
C32223.

RefSeq

NP_000871.1. NM_000880.3.
NP_001186815.1. NM_001199886.1.
NP_001186816.1. NM_001199887.1.
NP_001186817.1. NM_001199888.1.

UniGene

Hs.591873.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IL7	model	-	A	26-177	[»]
3DI2	X-ray	2.70	A/C	26-177	[»]
3DI3	X-ray	2.90	A	26-177	[»]

ProteinModelPortal

P13232.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3044N.

IntAct

P13232. 2 interactions.

STRING

9606.ENSP00000263851.

Polymorphism databases

DMDM

124354.

Proteomic databases

PaxDb

P13232.

PRIDE

P13232.

Protocols and materials databases

DNASU

3574.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263851; ENSP00000263851; ENSG00000104432.
ENST00000520269; ENSP00000427750; ENSG00000104432.

GeneID

3574.

KEGG

hsa:3574.

UCSC

uc003ybg.3. human.

Organism-specific databases

CTD

3574.

GeneCards

GC08M079587.

HGNC

HGNC:6023. IL7.

HPA

HPA019590.

MIM

146660. gene.

neXtProt

NX_P13232.

PharmGKB

PA29839.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG41411.

HOGENOM

HOG000036891.

HOVERGEN

HBG004609.

InParanoid

P13232.

KO

K05431.

OMA

CFLKILL.

OrthoDB

EOG4QNMXR.

Enzyme and pathway databases

Reactome

REACT_107772. Immune System.
REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P13232.

Bgee

P13232.

CleanEx

HS_IL7.

Genevestigator

P13232.

GermOnline

ENSG00000104432. Homo sapiens.

Family and domain databases

InterPro

IPR001181. Interleukin-7.
IPR018049. Interleukin-7/-9_CS.
IPR000226. Interleukin_7_9.
[Graphical view]

PANTHER

PTHR10526. PTHR10526. 1 hit.

Pfam

PF01415. IL7. 1 hit.
[Graphical view]

PIRSF

PIRSF001942. IL-7. 1 hit.

PRINTS

PR00435. INTERLEUKIN7.

SMART

SM00127. IL7. 1 hit.
[Graphical view]

PROSITE

PS00255. INTERLEUKIN_7_9. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

IL7. human.

EvolutionaryTrace

P13232.

GenomeRNAi

3574.

NextBio

13968.

SOURCE

Search...

Entry information

Entry name

IL7_HUMAN

Accession

Primary (citable) accession number: P13232
Secondary accession number(s): A0N0L3, Q5FBY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 130 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.