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Protein

Hisactophilin-1

Gene

hatA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as an intracellular pH sensor that links chemotactic signals to responses in the microfilament system of the cells by nucleating actin polymerization or stabilizing the filaments.

GO - Molecular functioni

  • actin filament binding Source: dictyBase
  • actin monomer binding Source: dictyBase
  • fatty acid binding Source: dictyBase
  • lipid binding Source: dictyBase
  • protein binding, bridging Source: InterPro

GO - Biological processi

  • actin filament polymerization Source: dictyBase

Keywordsi

Molecular functionActin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hisactophilin-1
Alternative name(s):
Histidine-rich actin-binding protein 1
Short name:
HS I
Gene namesi
Name:hatA
Synonyms:abpH
ORF Names:DDB_G0282141
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0282141. hatA.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: dictyBase
  • cytosol Source: dictyBase
  • phagocytic vesicle Source: dictyBase
  • plasma membrane Source: dictyBase

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000839042 – 118Hisactophilin-1Add BLAST117

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Disulfide bond, Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiP13231.

Interactioni

Subunit structurei

Homodimer or heterodimer of hatA and hatB, linked by a disulfide bond.

GO - Molecular functioni

  • actin filament binding Source: dictyBase
  • actin monomer binding Source: dictyBase
  • protein binding, bridging Source: InterPro

Structurei

Secondary structure

1118
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Beta strandi13 – 17Combined sources5
Beta strandi20 – 24Combined sources5
Beta strandi35 – 39Combined sources5
Beta strandi42 – 50Combined sources9
Beta strandi52 – 57Combined sources6
Beta strandi60 – 64Combined sources5
Beta strandi68 – 79Combined sources12
Beta strandi82 – 86Combined sources5
Helixi88 – 90Combined sources3
Beta strandi92 – 95Combined sources4
Helixi97 – 99Combined sources3
Beta strandi101 – 106Combined sources6
Beta strandi113 – 116Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HCDNMR-A1-118[»]
1HCENMR-A1-118[»]
ProteinModelPortaliP13231.
SMRiP13231.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13231.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati34 – 461Add BLAST13
Repeati74 – 862Add BLAST13

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 109Contains several HHXH repeatsAdd BLAST102
Regioni34 – 862 X 13 AA approximate repeatsAdd BLAST53

Sequence similaritiesi

Belongs to the hisactophilin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiP13231.
PhylomeDBiP13231.

Family and domain databases

CDDicd00257. Fascin. 1 hit.
InterProiView protein in InterPro
IPR008999. Actin_cross-linking.
IPR022768. Fascin-domain.
PfamiView protein in Pfam
PF06268. Fascin. 2 hits.
SUPFAMiSSF50405. SSF50405. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNRAFKSHH GHFLSAEGEA VKTHHGHHDH HTHFHVENHG GKVALKTHCG
60 70 80 90 100
KYLSIGDHKQ VYLSHHLHGD HSLFHLEHHG GKVSIKGHHH HYISADHHGH
110
VSTKEHHDHD TTFEEIII
Length:118
Mass (Da):13,456
Last modified:January 23, 2007 - v3
Checksum:iB5B43F527B694716
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04472 mRNA. Translation: AAA33218.1.
AAFI02000045 Genomic DNA. Translation: EAL66350.1.
PIRiA31429.
RefSeqiXP_640342.1. XM_635250.1.

Genome annotation databases

EnsemblProtistsiEAL66350; EAL66350; DDB_G0282141.
GeneIDi8623443.
KEGGiddi:DDB_G0282141.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHATA_DICDI
AccessioniPrimary (citable) accession number: P13231
Secondary accession number(s): Q54SW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 118 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families