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P13222

- CLAT_PIG

UniProt

P13222 - CLAT_PIG

Protein

Choline O-acetyltransferase

Gene

CHAT

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

    Catalytic activityi

    Acetyl-CoA + choline = CoA + O-acetylcholine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei335 – 3351Proton acceptorBy similarity
    Binding sitei451 – 4511Coenzyme ABy similarity
    Binding sitei552 – 5521Coenzyme ABy similarity

    GO - Molecular functioni

    1. choline O-acetyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. adult walking behavior Source: Ensembl
    2. dendrite development Source: Ensembl
    3. establishment of synaptic specificity at neuromuscular junction Source: Ensembl
    4. muscle organ development Source: Ensembl
    5. neuromuscular synaptic transmission Source: Ensembl
    6. neurotransmitter biosynthetic process Source: UniProtKB-KW
    7. rhythmic behavior Source: Ensembl
    8. rhythmic excitation Source: Ensembl

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Neurotransmitter biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_212730. Synthesis of PC.
    REACT_214957. Acetylcholine Neurotransmitter Release Cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline O-acetyltransferase (EC:2.3.1.6)
    Short name:
    CHOACTase
    Short name:
    ChAT
    Short name:
    Choline acetylase
    Gene namesi
    Name:CHAT
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 14

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. mitochondrion Source: Ensembl
    3. neuronal cell body Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 641640Choline O-acetyltransferasePRO_0000210156Add
    BLAST

    Proteomic databases

    PRIDEiP13222.

    Interactioni

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000011081.

    Structurei

    3D structure databases

    ProteinModelPortaliP13222.
    SMRiP13222. Positions 23-617.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni413 – 42513Coenzyme A bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG70127.
    GeneTreeiENSGT00740000115446.
    HOGENOMiHOG000233845.
    HOVERGENiHBG107717.
    KOiK00623.
    OMAiHVIVACC.
    OrthoDBiEOG7WHH90.
    TreeFamiTF313836.

    Family and domain databases

    InterProiIPR000542. Carn_acyl_trans.
    [Graphical view]
    PANTHERiPTHR22589. PTHR22589. 1 hit.
    PfamiPF00755. Carn_acyltransf. 1 hit.
    [Graphical view]
    PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
    PS00440. ACYLTRANSF_C_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13222-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPILEKTPPK MAAKSPSSEE EPGLPKLPVP PLQQTLATYL RCMQHLVPEE    50
    QFRRSQAIVQ QFGAPGGLGE TLQQKLLERQ EQTANWVSEY WLNDMYLNNR 100
    LALPVNSSPA VIFARQHFQD TNDQLRFAAN LISGVLSYKA LLDSHSIPID 150
    CAKGQLSGQP LCMKQYYGLF SSYRLPGHTQ DTLVAQKSSV MPEPEHVIVA 200
    CCNQFFVLDV VINFRRLSEG DLFTQLRKIV RMASNEDERL PPIGLLTSDG 250
    RSEWAEARTV LVKDSTNRDS LDMIERCICL VCLDAPGGME LSDTNRALQL 300
    LHGGGCSKNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL 350
    LKHMVKSSKK MVRADSVSEL PAPRRLRWKC SPEIQGLLAS SAEKLQQIVK 400
    NLDFTVYKFD DYGKTFIKQQ KCSPDAFIQV ALQLAFYRLH GRLVPTYESA 450
    SIRRFHEGRV DNIRSATPEA LHFVKAITDH ASAMPDSEKL LLLKDAIRAQ 500
    TQYTVMAITG MAIDNHLLGL RELAREVCKE LPEMFTDETY LMSNRFVLST 550
    SQVPTTMEMF CCYGPVVPNG YGACYNPQPE SILFCISSFH GCKETSSTKF 600
    AKAVEESFIE MKGLCSLSQS GMGKPLATKE KVTRPSQVHQ P 641
    Length:641
    Mass (Da):71,731
    Last modified:January 23, 2007 - v3
    Checksum:i90CFA6931F8CD393
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03021 mRNA. Translation: AAA31000.1.
    M27736 mRNA. Translation: AAA31015.1.
    PIRiA39961.
    RefSeqiNP_001001541.1. NM_001001541.1.
    UniGeneiSsc.16316.

    Genome annotation databases

    EnsembliENSSSCT00000011376; ENSSSCP00000011081; ENSSSCG00000010392.
    GeneIDi396896.
    KEGGissc:396896.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03021 mRNA. Translation: AAA31000.1 .
    M27736 mRNA. Translation: AAA31015.1 .
    PIRi A39961.
    RefSeqi NP_001001541.1. NM_001001541.1.
    UniGenei Ssc.16316.

    3D structure databases

    ProteinModelPortali P13222.
    SMRi P13222. Positions 23-617.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000011081.

    Proteomic databases

    PRIDEi P13222.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000011376 ; ENSSSCP00000011081 ; ENSSSCG00000010392 .
    GeneIDi 396896.
    KEGGi ssc:396896.

    Organism-specific databases

    CTDi 1103.

    Phylogenomic databases

    eggNOGi NOG70127.
    GeneTreei ENSGT00740000115446.
    HOGENOMi HOG000233845.
    HOVERGENi HBG107717.
    KOi K00623.
    OMAi HVIVACC.
    OrthoDBi EOG7WHH90.
    TreeFami TF313836.

    Enzyme and pathway databases

    Reactomei REACT_212730. Synthesis of PC.
    REACT_214957. Acetylcholine Neurotransmitter Release Cycle.

    Family and domain databases

    InterProi IPR000542. Carn_acyl_trans.
    [Graphical view ]
    PANTHERi PTHR22589. PTHR22589. 1 hit.
    Pfami PF00755. Carn_acyltransf. 1 hit.
    [Graphical view ]
    PROSITEi PS00439. ACYLTRANSF_C_1. 1 hit.
    PS00440. ACYLTRANSF_C_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and complete sequence of porcine choline acetyltransferase: in vitro translation of the corresponding RNA yields an active protein."
      Berrard S., Brice A., Lottspeich F., Braun A., Barde Y.-A., Mallet J.
      Proc. Natl. Acad. Sci. U.S.A. 84:9280-9284(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ventral spinal cord.
    2. "Molecular genetic approach to the study of mammalian choline acetyltransferase."
      Berrard S., Brice A., Mallet J.
      Brain Res. Bull. 22:147-153(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ventral spinal cord.
    3. "Comparison of the promoter region of the human and porcine choline acetyltransferase genes: localization of an important enhancer region."
      Hersh L.B., Kong C.F., Sampson C., Mues G., Li Y.P., Fisher A., Hilt D., Baetge E.E.
      J. Neurochem. 61:306-314(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-23.
    4. "N-terminal sequence of pig brain choline acetyltransferase purified by a rapid procedure."
      Braun A., Barde Y.-A., Lottspeich F., Mewes H.-W., Thoenen H.
      J. Neurochem. 48:16-21(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-12.
      Tissue: Brain.

    Entry informationi

    Entry nameiCLAT_PIG
    AccessioniPrimary (citable) accession number: P13222
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3