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Protein

Choline O-acetyltransferase

Gene

CHAT

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei335Proton acceptorBy similarity1
Binding sitei451Coenzyme ABy similarity1
Binding sitei552Coenzyme ABy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processNeurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiR-SSC-1483191 Synthesis of PC
R-SSC-264642 Acetylcholine Neurotransmitter Release Cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:CHAT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002101562 – 641Choline O-acetyltransferaseAdd BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineBy similarity1
Modified residuei366PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13222
PeptideAtlasiP13222
PRIDEiP13222

Expressioni

Gene expression databases

BgeeiENSSSCG00000010392
GenevisibleiP13222 SS

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011081

Structurei

3D structure databases

ProteinModelPortaliP13222
SMRiP13222
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni413 – 425Coenzyme A bindingBy similarityAdd BLAST13

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3717 Eukaryota
ENOG410XNZ9 LUCA
HOGENOMiHOG000233845
HOVERGENiHBG107717
InParanoidiP13222
KOiK00623
OMAiGEHSVMD
OrthoDBiEOG091G038F
TreeFamiTF313836

Family and domain databases

InterProiView protein in InterPro
IPR000542 Carn_acyl_trans
PANTHERiPTHR22589 PTHR22589, 1 hit
PfamiView protein in Pfam
PF00755 Carn_acyltransf, 1 hit
PROSITEiView protein in PROSITE
PS00439 ACYLTRANSF_C_1, 1 hit
PS00440 ACYLTRANSF_C_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPILEKTPPK MAAKSPSSEE EPGLPKLPVP PLQQTLATYL RCMQHLVPEE
60 70 80 90 100
QFRRSQAIVQ QFGAPGGLGE TLQQKLLERQ EQTANWVSEY WLNDMYLNNR
110 120 130 140 150
LALPVNSSPA VIFARQHFQD TNDQLRFAAN LISGVLSYKA LLDSHSIPID
160 170 180 190 200
CAKGQLSGQP LCMKQYYGLF SSYRLPGHTQ DTLVAQKSSV MPEPEHVIVA
210 220 230 240 250
CCNQFFVLDV VINFRRLSEG DLFTQLRKIV RMASNEDERL PPIGLLTSDG
260 270 280 290 300
RSEWAEARTV LVKDSTNRDS LDMIERCICL VCLDAPGGME LSDTNRALQL
310 320 330 340 350
LHGGGCSKNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL
360 370 380 390 400
LKHMVKSSKK MVRADSVSEL PAPRRLRWKC SPEIQGLLAS SAEKLQQIVK
410 420 430 440 450
NLDFTVYKFD DYGKTFIKQQ KCSPDAFIQV ALQLAFYRLH GRLVPTYESA
460 470 480 490 500
SIRRFHEGRV DNIRSATPEA LHFVKAITDH ASAMPDSEKL LLLKDAIRAQ
510 520 530 540 550
TQYTVMAITG MAIDNHLLGL RELAREVCKE LPEMFTDETY LMSNRFVLST
560 570 580 590 600
SQVPTTMEMF CCYGPVVPNG YGACYNPQPE SILFCISSFH GCKETSSTKF
610 620 630 640
AKAVEESFIE MKGLCSLSQS GMGKPLATKE KVTRPSQVHQ P
Length:641
Mass (Da):71,731
Last modified:January 23, 2007 - v3
Checksum:i90CFA6931F8CD393
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03021 mRNA Translation: AAA31000.1
M27736 mRNA Translation: AAA31015.1
PIRiA39961
RefSeqiNP_001001541.1, NM_001001541.1
UniGeneiSsc.16316

Genome annotation databases

GeneIDi396896
KEGGissc:396896

Similar proteinsi

Entry informationi

Entry nameiCLAT_PIG
AccessioniPrimary (citable) accession number: P13222
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 112 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health