Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Choline O-acetyltransferase

Gene

CHAT

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei335 – 3351Proton acceptorBy similarity
Binding sitei451 – 4511Coenzyme ABy similarity
Binding sitei552 – 5521Coenzyme ABy similarity

GO - Molecular functioni

  1. choline O-acetyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. adult walking behavior Source: Ensembl
  2. dendrite development Source: Ensembl
  3. establishment of synaptic specificity at neuromuscular junction Source: Ensembl
  4. muscle organ development Source: Ensembl
  5. neuromuscular synaptic transmission Source: Ensembl
  6. neurotransmitter biosynthetic process Source: UniProtKB-KW
  7. rhythmic behavior Source: Ensembl
  8. rhythmic excitation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Neurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiREACT_278794. Synthesis of PC.
REACT_339189. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:CHAT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

  1. axon Source: Ensembl
  2. mitochondrion Source: Ensembl
  3. neuronal cell body Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 641640Choline O-acetyltransferasePRO_0000210156Add
BLAST

Proteomic databases

PRIDEiP13222.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011081.

Structurei

3D structure databases

ProteinModelPortaliP13222.
SMRiP13222. Positions 23-617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 42513Coenzyme A bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
HOVERGENiHBG107717.
InParanoidiP13222.
KOiK00623.
OMAiHVIVACC.
OrthoDBiEOG7WHH90.
TreeFamiTF313836.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPILEKTPPK MAAKSPSSEE EPGLPKLPVP PLQQTLATYL RCMQHLVPEE
60 70 80 90 100
QFRRSQAIVQ QFGAPGGLGE TLQQKLLERQ EQTANWVSEY WLNDMYLNNR
110 120 130 140 150
LALPVNSSPA VIFARQHFQD TNDQLRFAAN LISGVLSYKA LLDSHSIPID
160 170 180 190 200
CAKGQLSGQP LCMKQYYGLF SSYRLPGHTQ DTLVAQKSSV MPEPEHVIVA
210 220 230 240 250
CCNQFFVLDV VINFRRLSEG DLFTQLRKIV RMASNEDERL PPIGLLTSDG
260 270 280 290 300
RSEWAEARTV LVKDSTNRDS LDMIERCICL VCLDAPGGME LSDTNRALQL
310 320 330 340 350
LHGGGCSKNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL
360 370 380 390 400
LKHMVKSSKK MVRADSVSEL PAPRRLRWKC SPEIQGLLAS SAEKLQQIVK
410 420 430 440 450
NLDFTVYKFD DYGKTFIKQQ KCSPDAFIQV ALQLAFYRLH GRLVPTYESA
460 470 480 490 500
SIRRFHEGRV DNIRSATPEA LHFVKAITDH ASAMPDSEKL LLLKDAIRAQ
510 520 530 540 550
TQYTVMAITG MAIDNHLLGL RELAREVCKE LPEMFTDETY LMSNRFVLST
560 570 580 590 600
SQVPTTMEMF CCYGPVVPNG YGACYNPQPE SILFCISSFH GCKETSSTKF
610 620 630 640
AKAVEESFIE MKGLCSLSQS GMGKPLATKE KVTRPSQVHQ P
Length:641
Mass (Da):71,731
Last modified:January 22, 2007 - v3
Checksum:i90CFA6931F8CD393
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03021 mRNA. Translation: AAA31000.1.
M27736 mRNA. Translation: AAA31015.1.
PIRiA39961.
RefSeqiNP_001001541.1. NM_001001541.1.
UniGeneiSsc.16316.

Genome annotation databases

EnsembliENSSSCT00000011376; ENSSSCP00000011081; ENSSSCG00000010392.
GeneIDi396896.
KEGGissc:396896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03021 mRNA. Translation: AAA31000.1.
M27736 mRNA. Translation: AAA31015.1.
PIRiA39961.
RefSeqiNP_001001541.1. NM_001001541.1.
UniGeneiSsc.16316.

3D structure databases

ProteinModelPortaliP13222.
SMRiP13222. Positions 23-617.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011081.

Proteomic databases

PRIDEiP13222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000011376; ENSSSCP00000011081; ENSSSCG00000010392.
GeneIDi396896.
KEGGissc:396896.

Organism-specific databases

CTDi1103.

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
HOVERGENiHBG107717.
InParanoidiP13222.
KOiK00623.
OMAiHVIVACC.
OrthoDBiEOG7WHH90.
TreeFamiTF313836.

Enzyme and pathway databases

ReactomeiREACT_278794. Synthesis of PC.
REACT_339189. Acetylcholine Neurotransmitter Release Cycle.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and complete sequence of porcine choline acetyltransferase: in vitro translation of the corresponding RNA yields an active protein."
    Berrard S., Brice A., Lottspeich F., Braun A., Barde Y.-A., Mallet J.
    Proc. Natl. Acad. Sci. U.S.A. 84:9280-9284(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ventral spinal cord.
  2. "Molecular genetic approach to the study of mammalian choline acetyltransferase."
    Berrard S., Brice A., Mallet J.
    Brain Res. Bull. 22:147-153(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ventral spinal cord.
  3. "Comparison of the promoter region of the human and porcine choline acetyltransferase genes: localization of an important enhancer region."
    Hersh L.B., Kong C.F., Sampson C., Mues G., Li Y.P., Fisher A., Hilt D., Baetge E.E.
    J. Neurochem. 61:306-314(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-23.
  4. "N-terminal sequence of pig brain choline acetyltransferase purified by a rapid procedure."
    Braun A., Barde Y.-A., Lottspeich F., Mewes H.-W., Thoenen H.
    J. Neurochem. 48:16-21(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-12.
    Tissue: Brain.

Entry informationi

Entry nameiCLAT_PIG
AccessioniPrimary (citable) accession number: P13222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 31, 1989
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.