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P13222

- CLAT_PIG

UniProt

P13222 - CLAT_PIG

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Protein
Choline O-acetyltransferase
Gene
CHAT
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei335 – 3351Proton acceptor By similarity
Binding sitei451 – 4511Coenzyme A By similarity
Binding sitei552 – 5521Coenzyme A By similarity

GO - Molecular functioni

  1. choline O-acetyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. adult walking behavior Source: Ensembl
  2. dendrite development Source: Ensembl
  3. establishment of synaptic specificity at neuromuscular junction Source: Ensembl
  4. muscle organ development Source: Ensembl
  5. neuromuscular synaptic transmission Source: Ensembl
  6. neurotransmitter biosynthetic process Source: UniProtKB-KW
  7. rhythmic behavior Source: Ensembl
  8. rhythmic excitation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Neurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiREACT_212730. Synthesis of PC.
REACT_214957. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:CHAT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 14

Subcellular locationi

GO - Cellular componenti

  1. axon Source: Ensembl
  2. mitochondrion Source: Ensembl
  3. neuronal cell body Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 641640Choline O-acetyltransferase
PRO_0000210156Add
BLAST

Proteomic databases

PRIDEiP13222.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011081.

Structurei

3D structure databases

ProteinModelPortaliP13222.
SMRiP13222. Positions 23-617.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 42513Coenzyme A binding By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00740000115446.
HOGENOMiHOG000233845.
HOVERGENiHBG107717.
KOiK00623.
OMAiHVIVACC.
OrthoDBiEOG7WHH90.
TreeFamiTF313836.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13222-1 [UniParc]FASTAAdd to Basket

« Hide

MPILEKTPPK MAAKSPSSEE EPGLPKLPVP PLQQTLATYL RCMQHLVPEE    50
QFRRSQAIVQ QFGAPGGLGE TLQQKLLERQ EQTANWVSEY WLNDMYLNNR 100
LALPVNSSPA VIFARQHFQD TNDQLRFAAN LISGVLSYKA LLDSHSIPID 150
CAKGQLSGQP LCMKQYYGLF SSYRLPGHTQ DTLVAQKSSV MPEPEHVIVA 200
CCNQFFVLDV VINFRRLSEG DLFTQLRKIV RMASNEDERL PPIGLLTSDG 250
RSEWAEARTV LVKDSTNRDS LDMIERCICL VCLDAPGGME LSDTNRALQL 300
LHGGGCSKNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL 350
LKHMVKSSKK MVRADSVSEL PAPRRLRWKC SPEIQGLLAS SAEKLQQIVK 400
NLDFTVYKFD DYGKTFIKQQ KCSPDAFIQV ALQLAFYRLH GRLVPTYESA 450
SIRRFHEGRV DNIRSATPEA LHFVKAITDH ASAMPDSEKL LLLKDAIRAQ 500
TQYTVMAITG MAIDNHLLGL RELAREVCKE LPEMFTDETY LMSNRFVLST 550
SQVPTTMEMF CCYGPVVPNG YGACYNPQPE SILFCISSFH GCKETSSTKF 600
AKAVEESFIE MKGLCSLSQS GMGKPLATKE KVTRPSQVHQ P 641
Length:641
Mass (Da):71,731
Last modified:January 23, 2007 - v3
Checksum:i90CFA6931F8CD393
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03021 mRNA. Translation: AAA31000.1.
M27736 mRNA. Translation: AAA31015.1.
PIRiA39961.
RefSeqiNP_001001541.1. NM_001001541.1.
UniGeneiSsc.16316.

Genome annotation databases

EnsembliENSSSCT00000011376; ENSSSCP00000011081; ENSSSCG00000010392.
GeneIDi396896.
KEGGissc:396896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03021 mRNA. Translation: AAA31000.1 .
M27736 mRNA. Translation: AAA31015.1 .
PIRi A39961.
RefSeqi NP_001001541.1. NM_001001541.1.
UniGenei Ssc.16316.

3D structure databases

ProteinModelPortali P13222.
SMRi P13222. Positions 23-617.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000011081.

Proteomic databases

PRIDEi P13222.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000011376 ; ENSSSCP00000011081 ; ENSSSCG00000010392 .
GeneIDi 396896.
KEGGi ssc:396896.

Organism-specific databases

CTDi 1103.

Phylogenomic databases

eggNOGi NOG70127.
GeneTreei ENSGT00740000115446.
HOGENOMi HOG000233845.
HOVERGENi HBG107717.
KOi K00623.
OMAi HVIVACC.
OrthoDBi EOG7WHH90.
TreeFami TF313836.

Enzyme and pathway databases

Reactomei REACT_212730. Synthesis of PC.
REACT_214957. Acetylcholine Neurotransmitter Release Cycle.

Family and domain databases

InterProi IPR000542. Carn_acyl_trans.
[Graphical view ]
PANTHERi PTHR22589. PTHR22589. 1 hit.
Pfami PF00755. Carn_acyltransf. 1 hit.
[Graphical view ]
PROSITEi PS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and complete sequence of porcine choline acetyltransferase: in vitro translation of the corresponding RNA yields an active protein."
    Berrard S., Brice A., Lottspeich F., Braun A., Barde Y.-A., Mallet J.
    Proc. Natl. Acad. Sci. U.S.A. 84:9280-9284(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ventral spinal cord.
  2. "Molecular genetic approach to the study of mammalian choline acetyltransferase."
    Berrard S., Brice A., Mallet J.
    Brain Res. Bull. 22:147-153(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ventral spinal cord.
  3. "Comparison of the promoter region of the human and porcine choline acetyltransferase genes: localization of an important enhancer region."
    Hersh L.B., Kong C.F., Sampson C., Mues G., Li Y.P., Fisher A., Hilt D., Baetge E.E.
    J. Neurochem. 61:306-314(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-23.
  4. "N-terminal sequence of pig brain choline acetyltransferase purified by a rapid procedure."
    Braun A., Barde Y.-A., Lottspeich F., Mewes H.-W., Thoenen H.
    J. Neurochem. 48:16-21(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-12.
    Tissue: Brain.

Entry informationi

Entry nameiCLAT_PIG
AccessioniPrimary (citable) accession number: P13222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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