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Protein

Choline O-acetyltransferase

Gene

CHAT

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei335Proton acceptorBy similarity1
Binding sitei451Coenzyme ABy similarity1
Binding sitei552Coenzyme ABy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processNeurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiR-SSC-1483191. Synthesis of PC.
R-SSC-264642. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:CHAT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 14

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002101562 – 641Choline O-acetyltransferaseAdd BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineBy similarity1
Modified residuei366PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13222.
PeptideAtlasiP13222.
PRIDEiP13222.

Expressioni

Gene expression databases

BgeeiENSSSCG00000010392.
GenevisibleiP13222. SS.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011081.

Structurei

3D structure databases

ProteinModelPortaliP13222.
SMRiP13222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni413 – 425Coenzyme A bindingBy similarityAdd BLAST13

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3717. Eukaryota.
ENOG410XNZ9. LUCA.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
HOVERGENiHBG107717.
InParanoidiP13222.
KOiK00623.
OMAiDKPMQFV.
OrthoDBiEOG091G038F.
TreeFamiTF313836.

Family and domain databases

InterProiView protein in InterPro
IPR000542. Carn_acyl_trans.
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiView protein in Pfam
PF00755. Carn_acyltransf. 1 hit.
PROSITEiView protein in PROSITE
PS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPILEKTPPK MAAKSPSSEE EPGLPKLPVP PLQQTLATYL RCMQHLVPEE
60 70 80 90 100
QFRRSQAIVQ QFGAPGGLGE TLQQKLLERQ EQTANWVSEY WLNDMYLNNR
110 120 130 140 150
LALPVNSSPA VIFARQHFQD TNDQLRFAAN LISGVLSYKA LLDSHSIPID
160 170 180 190 200
CAKGQLSGQP LCMKQYYGLF SSYRLPGHTQ DTLVAQKSSV MPEPEHVIVA
210 220 230 240 250
CCNQFFVLDV VINFRRLSEG DLFTQLRKIV RMASNEDERL PPIGLLTSDG
260 270 280 290 300
RSEWAEARTV LVKDSTNRDS LDMIERCICL VCLDAPGGME LSDTNRALQL
310 320 330 340 350
LHGGGCSKNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL
360 370 380 390 400
LKHMVKSSKK MVRADSVSEL PAPRRLRWKC SPEIQGLLAS SAEKLQQIVK
410 420 430 440 450
NLDFTVYKFD DYGKTFIKQQ KCSPDAFIQV ALQLAFYRLH GRLVPTYESA
460 470 480 490 500
SIRRFHEGRV DNIRSATPEA LHFVKAITDH ASAMPDSEKL LLLKDAIRAQ
510 520 530 540 550
TQYTVMAITG MAIDNHLLGL RELAREVCKE LPEMFTDETY LMSNRFVLST
560 570 580 590 600
SQVPTTMEMF CCYGPVVPNG YGACYNPQPE SILFCISSFH GCKETSSTKF
610 620 630 640
AKAVEESFIE MKGLCSLSQS GMGKPLATKE KVTRPSQVHQ P
Length:641
Mass (Da):71,731
Last modified:January 23, 2007 - v3
Checksum:i90CFA6931F8CD393
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03021 mRNA. Translation: AAA31000.1.
M27736 mRNA. Translation: AAA31015.1.
PIRiA39961.
RefSeqiNP_001001541.1. NM_001001541.1.
UniGeneiSsc.16316.

Genome annotation databases

EnsembliENSSSCT00000011376; ENSSSCP00000011081; ENSSSCG00000010392.
GeneIDi396896.
KEGGissc:396896.

Similar proteinsi

Entry informationi

Entry nameiCLAT_PIG
AccessioniPrimary (citable) accession number: P13222
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 109 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families