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P13222

- CLAT_PIG

UniProt

P13222 - CLAT_PIG

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Protein

Choline O-acetyltransferase

Gene

CHAT

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei335 – 3351Proton acceptorBy similarity
Binding sitei451 – 4511Coenzyme ABy similarity
Binding sitei552 – 5521Coenzyme ABy similarity

GO - Molecular functioni

  1. choline O-acetyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. adult walking behavior Source: Ensembl
  2. dendrite development Source: Ensembl
  3. establishment of synaptic specificity at neuromuscular junction Source: Ensembl
  4. muscle organ development Source: Ensembl
  5. neuromuscular synaptic transmission Source: Ensembl
  6. neurotransmitter biosynthetic process Source: UniProtKB-KW
  7. rhythmic behavior Source: Ensembl
  8. rhythmic excitation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Neurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiREACT_212730. Synthesis of PC.
REACT_214957. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:CHAT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 14

Subcellular locationi

GO - Cellular componenti

  1. axon Source: Ensembl
  2. mitochondrion Source: Ensembl
  3. neuronal cell body Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 641640Choline O-acetyltransferasePRO_0000210156Add
BLAST

Proteomic databases

PRIDEiP13222.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011081.

Structurei

3D structure databases

ProteinModelPortaliP13222.
SMRiP13222. Positions 23-617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 42513Coenzyme A bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
HOVERGENiHBG107717.
InParanoidiP13222.
KOiK00623.
OMAiHVIVACC.
OrthoDBiEOG7WHH90.
TreeFamiTF313836.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13222-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPILEKTPPK MAAKSPSSEE EPGLPKLPVP PLQQTLATYL RCMQHLVPEE
60 70 80 90 100
QFRRSQAIVQ QFGAPGGLGE TLQQKLLERQ EQTANWVSEY WLNDMYLNNR
110 120 130 140 150
LALPVNSSPA VIFARQHFQD TNDQLRFAAN LISGVLSYKA LLDSHSIPID
160 170 180 190 200
CAKGQLSGQP LCMKQYYGLF SSYRLPGHTQ DTLVAQKSSV MPEPEHVIVA
210 220 230 240 250
CCNQFFVLDV VINFRRLSEG DLFTQLRKIV RMASNEDERL PPIGLLTSDG
260 270 280 290 300
RSEWAEARTV LVKDSTNRDS LDMIERCICL VCLDAPGGME LSDTNRALQL
310 320 330 340 350
LHGGGCSKNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL
360 370 380 390 400
LKHMVKSSKK MVRADSVSEL PAPRRLRWKC SPEIQGLLAS SAEKLQQIVK
410 420 430 440 450
NLDFTVYKFD DYGKTFIKQQ KCSPDAFIQV ALQLAFYRLH GRLVPTYESA
460 470 480 490 500
SIRRFHEGRV DNIRSATPEA LHFVKAITDH ASAMPDSEKL LLLKDAIRAQ
510 520 530 540 550
TQYTVMAITG MAIDNHLLGL RELAREVCKE LPEMFTDETY LMSNRFVLST
560 570 580 590 600
SQVPTTMEMF CCYGPVVPNG YGACYNPQPE SILFCISSFH GCKETSSTKF
610 620 630 640
AKAVEESFIE MKGLCSLSQS GMGKPLATKE KVTRPSQVHQ P
Length:641
Mass (Da):71,731
Last modified:January 23, 2007 - v3
Checksum:i90CFA6931F8CD393
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03021 mRNA. Translation: AAA31000.1.
M27736 mRNA. Translation: AAA31015.1.
PIRiA39961.
RefSeqiNP_001001541.1. NM_001001541.1.
UniGeneiSsc.16316.

Genome annotation databases

EnsembliENSSSCT00000011376; ENSSSCP00000011081; ENSSSCG00000010392.
GeneIDi396896.
KEGGissc:396896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03021 mRNA. Translation: AAA31000.1 .
M27736 mRNA. Translation: AAA31015.1 .
PIRi A39961.
RefSeqi NP_001001541.1. NM_001001541.1.
UniGenei Ssc.16316.

3D structure databases

ProteinModelPortali P13222.
SMRi P13222. Positions 23-617.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000011081.

Proteomic databases

PRIDEi P13222.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000011376 ; ENSSSCP00000011081 ; ENSSSCG00000010392 .
GeneIDi 396896.
KEGGi ssc:396896.

Organism-specific databases

CTDi 1103.

Phylogenomic databases

eggNOGi NOG70127.
GeneTreei ENSGT00760000119220.
HOGENOMi HOG000233845.
HOVERGENi HBG107717.
InParanoidi P13222.
KOi K00623.
OMAi HVIVACC.
OrthoDBi EOG7WHH90.
TreeFami TF313836.

Enzyme and pathway databases

Reactomei REACT_212730. Synthesis of PC.
REACT_214957. Acetylcholine Neurotransmitter Release Cycle.

Family and domain databases

InterProi IPR000542. Carn_acyl_trans.
[Graphical view ]
PANTHERi PTHR22589. PTHR22589. 1 hit.
Pfami PF00755. Carn_acyltransf. 1 hit.
[Graphical view ]
PROSITEi PS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and complete sequence of porcine choline acetyltransferase: in vitro translation of the corresponding RNA yields an active protein."
    Berrard S., Brice A., Lottspeich F., Braun A., Barde Y.-A., Mallet J.
    Proc. Natl. Acad. Sci. U.S.A. 84:9280-9284(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ventral spinal cord.
  2. "Molecular genetic approach to the study of mammalian choline acetyltransferase."
    Berrard S., Brice A., Mallet J.
    Brain Res. Bull. 22:147-153(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ventral spinal cord.
  3. "Comparison of the promoter region of the human and porcine choline acetyltransferase genes: localization of an important enhancer region."
    Hersh L.B., Kong C.F., Sampson C., Mues G., Li Y.P., Fisher A., Hilt D., Baetge E.E.
    J. Neurochem. 61:306-314(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-23.
  4. "N-terminal sequence of pig brain choline acetyltransferase purified by a rapid procedure."
    Braun A., Barde Y.-A., Lottspeich F., Mewes H.-W., Thoenen H.
    J. Neurochem. 48:16-21(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-12.
    Tissue: Brain.

Entry informationi

Entry nameiCLAT_PIG
AccessioniPrimary (citable) accession number: P13222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3