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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

Gene

norpA

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes (By similarity). Essential component of the phototransduction pathway (PubMed:2457447). Essential downstream component of a hh-signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required for the activation of Cad99C and consequently Cad99C-dependent endosome formation, which is essential for the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection (PubMed:25639794).By similarity2 Publications

Caution

3D structural studies were performed with a synthetic heptapeptide that contained Cys, corresponding to position 1094, therefore the detected disulfide bridge is an artifact.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei334PROSITE-ProRule annotation1
Active sitei381PROSITE-ProRule annotation1
Binding sitei467SubstrateBy similarity1
Binding sitei469SubstrateBy similarity1
Binding sitei579SubstrateBy similarity1
Binding sitei606SubstrateBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • GTPase activator activity Source: FlyBase
  • phosphatidylinositol phospholipase C activity Source: FlyBase
  • phospholipase C activity Source: FlyBase
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • adult locomotory behavior Source: FlyBase
  • calcium-mediated signaling Source: FlyBase
  • cellular response to light stimulus Source: FlyBase
  • deactivation of rhodopsin mediated signaling Source: FlyBase
  • detection of chemical stimulus involved in sensory perception of bitter taste Source: FlyBase
  • diacylglycerol biosynthetic process Source: FlyBase
  • entrainment of circadian clock Source: FlyBase
  • entrainment of circadian clock by photoperiod Source: FlyBase
  • light-induced release of internally sequestered calcium ion Source: FlyBase
  • lipid catabolic process Source: UniProtKB-KW
  • mucosal immune response Source: FlyBase
  • negative regulation of compound eye retinal cell programmed cell death Source: FlyBase
  • phosphatidylinositol metabolic process Source: FlyBase
  • phospholipid biosynthetic process Source: FlyBase
  • phospholipid metabolic process Source: FlyBase
  • photoreceptor cell maintenance Source: FlyBase
  • phototransduction Source: FlyBase
  • positive regulation of clathrin-dependent endocytosis Source: FlyBase
  • positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: FlyBase
  • rhodopsin mediated signaling pathway Source: FlyBase
  • thermotaxis Source: FlyBase
  • visual perception Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism, Sensory transduction, Vision

Enzyme and pathway databases

ReactomeiR-DME-112043 PLC beta mediated events
R-DME-1855204 Synthesis of IP3 and IP4 in the cytosol
R-DME-416476 G alpha (q) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesteraseCurated (EC:3.1.4.11By similarity)
Alternative name(s):
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta1 PublicationImported
No receptor potential A protein1 Publication
Phosphoinositide phospholipase CCurated
Phosphoinositide phospholipase C-betaCurated
Gene namesi
Name:norpAImported
Synonyms:PLC-beta1 PublicationImported
ORF Names:CG3620Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0262738 norpA

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Flies have no photoreceptor potential, their eyes lack phospholipase C (PLC) activity and they are completely blind.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000885101 – 10951-phosphatidylinositol 4,5-bisphosphate phosphodiesteraseAdd BLAST1095

Proteomic databases

PaxDbiP13217
PRIDEiP13217

Expressioni

Tissue specificityi

Abundantly expressed in the adult retina.1 Publication

Gene expression databases

BgeeiFBgn0262738
ExpressionAtlasiP13217 differential
GenevisibleiP13217 DM

Interactioni

Subunit structurei

Interacts with inaD.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
inaDQ240084EBI-101510,EBI-195326

Protein-protein interaction databases

BioGridi57893, 29 interactors
DIPiDIP-45N
IntActiP13217, 10 interactors
MINTiP13217
STRINGi7227.FBpp0070618

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IHJX-ray1.80A/B211-214[»]
ProteinModelPortaliP13217
SMRiP13217
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13217

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini319 – 469PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST151
Domaini550 – 666PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini673 – 771C2PROSITE-ProRule annotationAdd BLAST99

Phylogenomic databases

eggNOGiKOG1265 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
InParanoidiP13217
KOiK05858
OMAiFTYMVAE
OrthoDBiEOG091G00XL
PhylomeDBiP13217

Family and domain databases

CDDicd13361 PH_PLC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR009535 PLC-beta_CS
IPR037862 PLC-beta_PH
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF06631 DUF1154, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P13217-1) [UniParc]FASTAAdd to basket
Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKKYEFDWI IPVPPELTTG CVFDRWFENE KETKENDFER DALFKVDEYG
60 70 80 90 100
FFLYWKSEGR DGDVIELCQV SDIRAGGTPK DPKILDKVTK KNGTNIPELD
110 120 130 140 150
KRSLTICSNT DYINITYHHV ICPDAATAKS WQKNLRLITH NNRATNVCPR
160 170 180 190 200
VNLMKHWMRL SYCVEKSGKI PVKTLAKTFA SGKTEKLVYT CIKDAGLPDD
210 220 230 240 250
KNATMTKEQF TFDKFYALYH KVCPRNDIEE LFTSITKGKQ DFISLEQFIQ
260 270 280 290 300
FMNDKQRDPR MNEILYPLYE EKRCTEIIND YELDEEKKKN VQMSLDGFKR
310 320 330 340 350
YLMSDENAPV FLDRLDFYME MDQPLAHYYI NSSHNTYLSG RQIGGKSSVE
360 370 380 390 400
MYRQTLLAGC RCVELDCWNG KGEDEEPIVT HGHAYCTEIL FKDCIQAIAD
410 420 430 440 450
CAFVSSEYPV ILSFENHCNR AQQYKLAKYC DDFFGDLLLK EPLPDHPLDP
460 470 480 490 500
GLPLPPPCKL KRKILIKNKR MKPEVEKVEL ELWLKGELKT DDDPEEDASA
510 520 530 540 550
GKPPEAAAAP APAPEAAAAA EGAAEGGGGA EAEAAAANYS GSTTNVHPWL
560 570 580 590 600
SSMVNYAQPI KFQGFDKAIE KNIAHNMSSF AESAGMNYLK QSSIDFVNYN
610 620 630 640 650
KRQMSRIYPK GTRADSSNYM PQVFWNAGCQ MVSLNFQSSD LPMQLNQGKF
660 670 680 690 700
EYNGGCGYLL KPDFMRRADK DFDPFADAPV DGVIAAQCSV KVIAGQFLSD
710 720 730 740 750
KKVGTYVEVD MFGLPSDTVK KEFRTRLVAN NGLNPVYNED PFVFRKVVLP
760 770 780 790 800
DLAVLRFGVY EESGKILGQR ILPLDGLQAG YRHVSLRTEA NFPMSLPMLF
810 820 830 840 850
VNIELKIYVP DGFEDFMAML SDPRGFAGAA KQQNEQMKAL GIEEQSGGAA
860 870 880 890 900
RDAGKAKEEE KKEPPLVFEP VTLESLRQEK GFQKVGKKQI KELDTLRKKH
910 920 930 940 950
AKERTSVQKT QNAAIDKLIK GKSKDDIRND ANIKNSINDQ TKQWTDMIAR
960 970 980 990 1000
HRKEEWDMLR QHVQDSQDAM KALMLTVQAA QIKQLEDRHA RDIKDLNAKQ
1010 1020 1030 1040 1050
AKMSADTAKE VQNDKTLKTK NEKDRRLREK RQNNVKRFME EKKQIGVKQG
1060 1070 1080 1090
RAMEKLKLAH SKQIEEFSTD VQKLMDMYKI EEEAYKTQGK TEFYA
Length:1,095
Mass (Da):124,863
Last modified:June 10, 2008 - v4
Checksum:iCC712ABF9B065BB6
GO
Isoform C (identifier: P13217-2) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     130-154: SWQKNLRLITHNNRATNVCPRVNLM → IWLDGIRKITHNVKANNICPMMCLR

Note: No experimental confirmation available.
Show »
Length:1,095
Mass (Da):124,824
Checksum:i6F09204041C4FF74
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti446H → R in AAA28724 (PubMed:2457447).Curated1
Sequence conflicti968D → G in AAO25053 (Ref. 5) Curated1
Sequence conflicti1094Y → C in AAA28724 (PubMed:2457447).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034088130 – 154SWQKN…RVNLM → IWLDGIRKITHNVKANNICP MMCLR in isoform C. CuratedAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03138 mRNA Translation: AAA28724.1
AE014298 Genomic DNA Translation: AAF45942.2
AE014298 Genomic DNA Translation: AAN09121.1
AE014298 Genomic DNA Translation: AAX52474.1
AE014298 Genomic DNA Translation: AAX52475.1
AF181641 mRNA Translation: AAD55427.1
BT003293 mRNA Translation: AAO25053.1
PIRiA31225
RefSeqiNP_001014720.1, NM_001014720.2 [P13217-2]
NP_001014721.1, NM_001014721.3 [P13217-2]
NP_001162661.1, NM_001169190.2 [P13217-1]
NP_001284860.1, NM_001297931.1 [P13217-2]
NP_525069.2, NM_080330.4 [P13217-1]
NP_726925.1, NM_167008.2 [P13217-1]
UniGeneiDm.7394

Genome annotation databases

EnsemblMetazoaiFBtr0070650; FBpp0070618; FBgn0262738 [P13217-1]
FBtr0070651; FBpp0070619; FBgn0262738 [P13217-1]
FBtr0100670; FBpp0100136; FBgn0262738 [P13217-2]
FBtr0100671; FBpp0100137; FBgn0262738 [P13217-2]
FBtr0301475; FBpp0290690; FBgn0262738 [P13217-1]
FBtr0343599; FBpp0310196; FBgn0262738 [P13217-2]
GeneIDi31376
KEGGidme:Dmel_CG3620

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPIPA_DROME
AccessioniPrimary (citable) accession number: P13217
Secondary accession number(s): A4V3Y2
, Q59E70, Q86P93, Q9U4G4, Q9W4K9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 10, 2008
Last modified: March 28, 2018
This is version 196 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health