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Protein

Annexin A4

Gene

ANXA4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in alveolar type II cells through interaction with the surfactant protein SFTPA1 (SP-A).

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB-KW
  • calcium-dependent protein binding Source: AgBase
  • calcium ion binding Source: Ensembl
  • carbohydrate binding Source: AgBase
  • chondroitin sulfate binding Source: AgBase
  • heparin binding Source: AgBase
  • identical protein binding Source: AgBase

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Annexin A4
Alternative name(s):
35-beta calcimedin
Annexin IV
Annexin-4
Carbohydrate-binding protein p33/p41
Chromobindin-4
Endonexin I
Lipocortin IV
P32.5
PP4-X
Placental anticoagulant protein II
Short name:
PAP-II
Protein II
Gene namesi
Name:ANXA4
Synonyms:ANX4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: AgBase
  • cell surface Source: Ensembl
  • chromaffin granule membrane Source: AgBase
  • cytosol Source: AgBase
  • extracellular exosome Source: Ensembl
  • membrane Source: AgBase
  • nuclear membrane Source: Ensembl
  • nucleus Source: AgBase
  • perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 319318Annexin A4PRO_0000067480Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei7 – 71PhosphothreonineBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Cross-linki28 – 28Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki28 – 28Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei69 – 691N6-acetyllysineBy similarity
Modified residuei100 – 1001N6-acetyllysineBy similarity
Modified residuei213 – 2131N6-acetyllysineBy similarity
Modified residuei293 – 2931N6-acetyllysineBy similarity
Modified residuei300 – 3001N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP13214.
PeptideAtlasiP13214.
PRIDEiP13214.

Expressioni

Gene expression databases

BgeeiENSBTAG00000001105.

Interactioni

Subunit structurei

Monomer. Binds to SFTPA1 in a Ca2+-dependent manner.

GO - Molecular functioni

  • calcium-dependent protein binding Source: AgBase
  • identical protein binding Source: AgBase

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001463.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 2712Combined sources
Beta strandi28 – 314Combined sources
Helixi34 – 418Combined sources
Helixi46 – 6015Combined sources
Helixi64 – 718Combined sources
Helixi74 – 8411Combined sources
Helixi87 – 9913Combined sources
Beta strandi100 – 1034Combined sources
Helixi106 – 11510Combined sources
Helixi118 – 13215Combined sources
Helixi136 – 1438Combined sources
Helixi146 – 15611Combined sources
Helixi168 – 18114Combined sources
Turni184 – 1863Combined sources
Helixi190 – 19910Combined sources
Helixi202 – 21615Combined sources
Helixi220 – 2278Combined sources
Helixi230 – 25829Combined sources
Beta strandi259 – 2624Combined sources
Helixi265 – 27511Combined sources
Turni276 – 2783Combined sources
Helixi280 – 29112Combined sources
Helixi295 – 3028Combined sources
Helixi305 – 31511Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ANNX-ray2.30A2-319[»]
1AOWX-ray3.00A11-319[»]
1I4AX-ray2.00A2-319[»]
ProteinModelPortaliP13214.
SMRiP13214. Positions 5-319.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13214.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati23 – 8361Annexin 1Add
BLAST
Repeati95 – 15561Annexin 2Add
BLAST
Repeati179 – 23961Annexin 3Add
BLAST
Repeati254 – 31461Annexin 4Add
BLAST

Domaini

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similaritiesi

Belongs to the annexin family.Curated
Contains 4 annexin repeats.Curated

Keywords - Domaini

Annexin, Repeat

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP13214.
KOiK17093.
OMAiGMMMPTV.
OrthoDBiEOG091G0H6H.
TreeFamiTF105452.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002391. AnnexinIV.
[Graphical view]
PANTHERiPTHR10502:SF28. PTHR10502:SF28. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKGGTVKA ASGFNAAEDA QTLRKAMKGL GTDEDAIINV LAYRSTAQRQ
60 70 80 90 100
EIRTAYKTTI GRDLMDDLKS ELSGNFEQVI LGMMTPTVLY DVQELRKAMK
110 120 130 140 150
GAGTDEGCLI EILASRTPEE IRRINQTYQL QYGRSLEDDI RSDTSFMFQR
160 170 180 190 200
VLVSLSAGGR DESNYLDDAL MRQDAQDLYE AGEKKWGTDE VKFLTVLCSR
210 220 230 240 250
NRNHLLHVFD EYKRIAQKDI EQSIKSETSG SFEDALLAIV KCMRNKSAYF
260 270 280 290 300
AERLYKSMKG LGTDDDTLIR VMVSRAEIDM LDIRANFKRL YGKSLYSFIK
310
GDTSGDYRKV LLILCGGDD
Length:319
Mass (Da):35,889
Last modified:January 23, 2007 - v2
Checksum:iA795DD72B0EE961F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951L → V in BAA11243 (PubMed:8631806).Curated
Sequence conflicti198 – 1981C → Y AA sequence (PubMed:1400371).Curated
Sequence conflicti211 – 2111E → K in BAA11243 (PubMed:8631806).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22248 mRNA. Translation: AAA30507.1.
X13627 mRNA. Translation: CAA31954.1.
D78178 mRNA. Translation: BAA11243.1.
BC103381 mRNA. Translation: AAI03382.1.
PIRiA31578. LUBO4.
RefSeqiNP_001001440.2. NM_001001440.2.
UniGeneiBt.38197.

Genome annotation databases

EnsembliENSBTAT00000001463; ENSBTAP00000001463; ENSBTAG00000001105.
GeneIDi281625.
KEGGibta:281625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22248 mRNA. Translation: AAA30507.1.
X13627 mRNA. Translation: CAA31954.1.
D78178 mRNA. Translation: BAA11243.1.
BC103381 mRNA. Translation: AAI03382.1.
PIRiA31578. LUBO4.
RefSeqiNP_001001440.2. NM_001001440.2.
UniGeneiBt.38197.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ANNX-ray2.30A2-319[»]
1AOWX-ray3.00A11-319[»]
1I4AX-ray2.00A2-319[»]
ProteinModelPortaliP13214.
SMRiP13214. Positions 5-319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001463.

Proteomic databases

PaxDbiP13214.
PeptideAtlasiP13214.
PRIDEiP13214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000001463; ENSBTAP00000001463; ENSBTAG00000001105.
GeneIDi281625.
KEGGibta:281625.

Organism-specific databases

CTDi307.

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP13214.
KOiK17093.
OMAiGMMMPTV.
OrthoDBiEOG091G0H6H.
TreeFamiTF105452.

Miscellaneous databases

EvolutionaryTraceiP13214.

Gene expression databases

BgeeiENSBTAG00000001105.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002391. AnnexinIV.
[Graphical view]
PANTHERiPTHR10502:SF28. PTHR10502:SF28. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANXA4_BOVIN
AccessioniPrimary (citable) accession number: P13214
Secondary accession number(s): Q3SYU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Seems to bind one calcium ion with high affinity.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.