Reviewed, UniProtKB/Swiss-Prot P13212 (GAL7_STRLI)

Names and origin

Protein names

Recommended name:
    Galactose-1-phosphate uridylyltransferase
      Short name=Gal-1-P uridylyltransferase
    EC=2.7.7.12
Alternative name(s):
    UDP-glucose--hexose-1-phosphate uridylyltransferase

Gene names

Name:

galT

Organism

Streptomyces lividans

Taxonomic identifier

1916 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	UDP-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-galactose.
Cofactor	Binds 1 iron ion per subunit Potential. Binds 1 zinc ion per subunit Potential.
Pathway	Carbohydrate metabolism; galactose metabolism.
Sequence similarities	Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.
Sequence caution	The sequence AAA26746.1 differs from that shown. Reason: Frameshift at positions 59, 75, 307 and 354.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Ligand	Iron Metal-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-glucose:hexose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Galactose-1-phosphate uridylyltransferase		PRO_0000169897
Sites
	Active site	167	1	Tele-UMP-histidine intermediate By similarity
	Metal binding	114	1	Zinc Potential
	Metal binding	165	1	Zinc Potential
	Metal binding	305	1	Iron Potential
	Metal binding	307	1	Iron Potential
Experimental info
	Sequence conflict	59 – 75	17	QGRTY…PLCPS → AGAHLPSAGRPVPAVPV in AAA26746. Ref.1
	Sequence conflict	307 – 354	48	NFSLP…VHERE → ELFTSAVRPAS in AAA26746. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13212-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 73B3BA8C2DAC9BA2	FASTA	354	40,095
	10 20 30 40 50 60 MKKTSTRLAD GRELVYYDLR DDTVRDAVDR RPLERTVTTS EVRRDPLLGD SAPSRLAPQG 70 80 90 100 110 120 RTYHPPADQC PLCPSGRGTA ERDPAYDVVV FENRFPSLAG DSGRCEVVCF TSDHDASFAD 130 140 150 160 170 180 LSEEQARLVV DAWTDRTSEL SHLPSVEQVF CFENRGAEIG VTLGHPHGQI YAYPFTTPRT 190 200 210 220 230 240 ALMLRSLAAH KDATGGGNLF DSVLEEELAG ERVVLEGEHW AAFVAYGAHW PYEVHLYPKR 250 260 270 280 290 300 RVPDLLGLDE AARTEFPKVY LELLRRFDRI FGEGEPPTPY IAAWHQAPFG QLEFEGVTRD 310 320 330 340 350 DFALHLNFSL PPYVRQAEVP RGLRIRHERV HQRDVPPERA AERLREVADV HERE

References

[1]	"Gene organization and structure of the Streptomyces lividans gal operon." Adams C.W., Fornwald J.A., Schmidt F.J., Rosenberg M., Brawner M.E. J. Bacteriol. 170:203-212(1988) [PubMed: 3335481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Gibson T.J. Unpublished observations (MAR-1995) Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFTS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M18953 Genomic DNA. Translation: AAA26746.1. Frameshift.
PIR	XNSMUD. A28669.
3D structure databases
HSSP	HSSP built from PDB template 1HXP based on UniProtKB P09148.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.12. 15238.
Family and domain databases
InterPro	IPR001937. GalP_UDPtransf1. IPR019779. GalP_UDPtransf1_His-AS. IPR005850. GalP_Utransf_C. IPR005849. GalP_Utransf_N. IPR011151. His_triad_motif. [Graphical view]
Gene3D	G3DSA:3.30.428.10. His_triad_motif. 2 hits.
PANTHER	PTHR11943. GalP_UDPtransf1. 1 hit.
Pfam	PF02744. GalP_UDP_tr_C. 1 hit. PF01087. GalP_UDP_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000808. GalT. 1 hit.
ProDom	PD005051. GalP_Utransf. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00209. galT_1. 1 hit.
PROSITE	PS00117. GAL_P_UDP_TRANSF_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAL7_STRLI

Accession

Primary (citable) accession number: P13212

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)