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Protein

Sarafotoxin

Gene
N/A
Organism
Atractaspis engaddensis (Israeli burrowing asp) (Israeli mole viper)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm.1 Publication
Sarafotoxin-B: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays high agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the picomolar range) and endothelin-1 receptor (EDNRA) (lower affinities) (PubMed:21889567).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei90Endothelin-receptor binding siteBy similarity1
Sitei130Endothelin-receptor binding siteBy similarity1
Sitei170Endothelin-receptor binding siteBy similarity1
Sitei210Endothelin-receptor binding siteBy similarity1
Sitei250Endothelin-receptor binding siteBy similarity1
Sitei290Endothelin-receptor binding siteBy similarity1
Sitei330Endothelin-receptor binding siteBy similarity1
Sitei370Endothelin-receptor binding siteBy similarity1
Sitei410Endothelin-receptor binding siteBy similarity1
Sitei450Endothelin-receptor binding siteBy similarity1
Sitei490Endothelin-receptor binding siteBy similarity1
Sitei530Endothelin-receptor binding siteBy similarity1

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cardiotoxin, G-protein coupled receptor impairing toxin, Toxin, Vasoactive, Vasoconstrictor

Names & Taxonomyi

Protein namesi
Recommended name:
Sarafotoxin
Cleaved into the following 5 chains:
Sarafotoxin-A, Ser-isoform
Short name:
SRTX-A
Short name:
Sarafotoxin-A
Alternative name(s):
S6A
Sarafotoxin-C
Short name:
SRTX-C
Alternative name(s):
S6C
Sarafotoxin-B
Short name:
SRTX-B
Alternative name(s):
S6B
Sarafotoxin-E
Short name:
SRTX-E
Alternative name(s):
S6E
OrganismiAtractaspis engaddensis (Israeli burrowing asp) (Israeli mole viper)
Taxonomic identifieri1343144 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaLamprophiidaeAtractaspidinaeAtractaspis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

Sarafotoxin-A and sarafotoxin-B: LD50 is 0.015 mg/kg by intravenous injection into mice.2 Publications
Sarafotoxin-C: LD50 is 0.3 mg/kg by intravenous injection into mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170W → WVNRN or WDEP: Drastic decrease in affinity for ET-B receptors (3 and 5-orders or magnitude for VNRN and DEP, respectively). 1 Publication1
Mutagenesisi450W → WVNRN or DEP: Drastic decrease in affinity for ET-B receptors (3 and 5-orders or magnitude for VNRN and DEP, respectively). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000000812324 – 69Add BLAST46
PeptideiPRO_000000812470 – 90Sarafotoxin-A, Ser-isoformAdd BLAST21
PropeptideiPRO_000000812592 – 109Add BLAST18
PeptideiPRO_0000008126110 – 130Sarafotoxin-CAdd BLAST21
PropeptideiPRO_0000008127132 – 149Add BLAST18
PeptideiPRO_0000008128150 – 170Sarafotoxin-BAdd BLAST21
PropeptideiPRO_0000008129172 – 189Add BLAST18
PeptideiPRO_0000008130190 – 210Sarafotoxin-CAdd BLAST21
PropeptideiPRO_0000008131212 – 229Add BLAST18
PeptideiPRO_0000008132230 – 250Sarafotoxin-CAdd BLAST21
PropeptideiPRO_0000008133252 – 269Add BLAST18
PeptideiPRO_0000008134270 – 290Sarafotoxin-EAdd BLAST21
PropeptideiPRO_0000008135292 – 309Add BLAST18
PeptideiPRO_0000008136310 – 330Sarafotoxin-A, Ser-isoformAdd BLAST21
PropeptideiPRO_0000008137332 – 349Add BLAST18
PeptideiPRO_0000008138350 – 370Sarafotoxin-CAdd BLAST21
PropeptideiPRO_0000008139372 – 389Add BLAST18
PeptideiPRO_0000008140390 – 410Sarafotoxin-CAdd BLAST21
PropeptideiPRO_0000008141412 – 429Add BLAST18
PeptideiPRO_0000008142430 – 450Sarafotoxin-BAdd BLAST21
PropeptideiPRO_0000008143452 – 469Add BLAST18
PeptideiPRO_0000008144470 – 490Sarafotoxin-A, Ser-isoformAdd BLAST21
PropeptideiPRO_0000008145492 – 509Add BLAST18
PeptideiPRO_0000008146510 – 530Sarafotoxin-A, Thr-isoformAdd BLAST21
PropeptideiPRO_0000008147532 – 543Add BLAST12

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 84By similarity
Disulfide bondi72 ↔ 80By similarity
Disulfide bondi110 ↔ 124By similarity
Disulfide bondi112 ↔ 120By similarity
Disulfide bondi150 ↔ 1641 Publication
Disulfide bondi152 ↔ 1601 Publication
Disulfide bondi190 ↔ 204By similarity
Disulfide bondi192 ↔ 200By similarity
Disulfide bondi230 ↔ 244By similarity
Disulfide bondi232 ↔ 240By similarity
Disulfide bondi270 ↔ 284By similarity
Disulfide bondi272 ↔ 280By similarity
Disulfide bondi310 ↔ 324By similarity
Disulfide bondi312 ↔ 320By similarity
Disulfide bondi350 ↔ 364By similarity
Disulfide bondi352 ↔ 360By similarity
Disulfide bondi390 ↔ 404By similarity
Disulfide bondi392 ↔ 400By similarity
Disulfide bondi430 ↔ 4441 Publication
Disulfide bondi432 ↔ 4401 Publication
Disulfide bondi470 ↔ 484By similarity
Disulfide bondi472 ↔ 480By similarity
Disulfide bondi510 ↔ 524By similarity
Disulfide bondi512 ↔ 520By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1543
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi433 – 435Combined sources3
Helixi438 – 444Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SRBNMR-A430-450[»]
ProteinModelPortaliP13208.
SMRiP13208.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13208.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati51 – 901Add BLAST40
Repeati91 – 1302Add BLAST40
Repeati131 – 1703Add BLAST40
Repeati171 – 2104Add BLAST40
Repeati211 – 2505Add BLAST40
Repeati251 – 2906Add BLAST40
Repeati291 – 3307Add BLAST40
Repeati331 – 3708Add BLAST40
Repeati371 – 4109Add BLAST40
Repeati411 – 45010Add BLAST40
Repeati451 – 49011Add BLAST40
Repeati491 – 53012Add BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 53012 X 40 AA tandem repeatsAdd BLAST480

Sequence similaritiesi

Belongs to the endothelin/sarafotoxin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG017852.

Family and domain databases

InterProiIPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 12 hits.
[Graphical view]
SMARTiSM00272. END. 12 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 12 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLPRLAAG GLLLLLALAA LEGKPAPSAL SQLLEKRSED QAAAGRIIDG
60 70 80 90 100
GDTKQAARDP SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP
110 120 130 140 150
SPQRNVEPLC TCNDMTDEEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC
160 170 180 190 200
SCKDMTDKEC LYFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC
210 220 230 240 250
LNFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC LNFCHQDVIW
260 270 280 290 300
RDTKQAARDP SPQRNVEPLC TCKDMTDKEC LYFCHQGIIW RDTKQAARDP
310 320 330 340 350
SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC
360 370 380 390 400
TCNDMTDEEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC
410 420 430 440 450
LNFCHQDVIW RDTKQAARDP SPQRNVEPLC SCKDMTDKEC LYFCHQDVIW
460 470 480 490 500
RDTKQAARDP SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP
510 520 530 540
SPQRNVEPLC SCKDMTDKEC LNFCHQDVIW KNADTSANPE FLG
Length:543
Mass (Da):62,326
Last modified:February 1, 1994 - v2
Checksum:iDF84A9631392FE4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07528 mRNA. Translation: AAA48515.1.
D13322 Genomic DNA. Translation: BAA02579.2.
PIRiA46601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07528 mRNA. Translation: AAA48515.1.
D13322 Genomic DNA. Translation: BAA02579.2.
PIRiA46601.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SRBNMR-A430-450[»]
ProteinModelPortaliP13208.
SMRiP13208.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG017852.

Miscellaneous databases

EvolutionaryTraceiP13208.

Family and domain databases

InterProiIPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 12 hits.
[Graphical view]
SMARTiSM00272. END. 12 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 12 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRTX_ATREN
AccessioniPrimary (citable) accession number: P13208
Secondary accession number(s): P13209, P13210
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.