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P13208

- SRTX_ATREN

UniProt

P13208 - SRTX_ATREN

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Protein
Sarafotoxin
Gene
N/A
Organism
Atractaspis engaddensis (Israeli burrowing asp) (Israeli mole viper)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm.
Sarafotoxin-B: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm By similarity. Displays high agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the picomolar range) and endothelin-1 receptor (EDNRA) (lower affinities) (1 Publication).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei90 – 901Endothelin-receptor binding site By similarity
Sitei130 – 1301Endothelin-receptor binding site By similarity
Sitei170 – 1701Endothelin-receptor binding site By similarity
Sitei210 – 2101Endothelin-receptor binding site By similarity
Sitei250 – 2501Endothelin-receptor binding site By similarity
Sitei290 – 2901Endothelin-receptor binding site By similarity
Sitei330 – 3301Endothelin-receptor binding site By similarity
Sitei370 – 3701Endothelin-receptor binding site By similarity
Sitei410 – 4101Endothelin-receptor binding site By similarity
Sitei450 – 4501Endothelin-receptor binding site By similarity
Sitei490 – 4901Endothelin-receptor binding site By similarity
Sitei530 – 5301Endothelin-receptor binding site By similarity

GO - Biological processi

  1. regulation of vasoconstriction Source: InterPro
  2. vasoconstriction Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Cardiotoxin, G-protein coupled receptor impairing toxin, Toxin, Vasoactive, Vasoconstrictor

Names & Taxonomyi

Protein namesi
Recommended name:
Sarafotoxin
Cleaved into the following 5 chains:
Sarafotoxin-A, Ser-isoform
Short name:
SRTX-A
Short name:
Sarafotoxin-A
Alternative name(s):
S6A
Sarafotoxin-C
Short name:
SRTX-C
Alternative name(s):
S6C
Sarafotoxin-B
Short name:
SRTX-B
Alternative name(s):
S6B
Sarafotoxin-E
Short name:
SRTX-E
Alternative name(s):
S6E
OrganismiAtractaspis engaddensis (Israeli burrowing asp) (Israeli mole viper)
Taxonomic identifieri8600 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaAtractaspididaeAtractaspis

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

Sarafotoxin-A and sarafotoxin-B: LD50 is 0.015 mg/kg by intravenous injection into mice (1 Publication).1 Publication
Sarafotoxin-C: LD50 is 0.3 mg/kg by intravenous injection into mice.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi170 – 1701W → WVNRN or WDEP: Drastic decrease in affinity for ET-B receptors (3 and 5-orders or magnitude for VNRN and DEP, respectively). 1 Publication
Mutagenesisi450 – 4501W → WVNRN or DEP: Drastic decrease in affinity for ET-B receptors (3 and 5-orders or magnitude for VNRN and DEP, respectively). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Propeptidei24 – 6946
PRO_0000008123Add
BLAST
Peptidei70 – 9021Sarafotoxin-A, Ser-isoform
PRO_0000008124Add
BLAST
Propeptidei92 – 10918
PRO_0000008125Add
BLAST
Peptidei110 – 13021Sarafotoxin-C
PRO_0000008126Add
BLAST
Propeptidei132 – 14918
PRO_0000008127Add
BLAST
Peptidei150 – 17021Sarafotoxin-B
PRO_0000008128Add
BLAST
Propeptidei172 – 18918
PRO_0000008129Add
BLAST
Peptidei190 – 21021Sarafotoxin-C
PRO_0000008130Add
BLAST
Propeptidei212 – 22918
PRO_0000008131Add
BLAST
Peptidei230 – 25021Sarafotoxin-C
PRO_0000008132Add
BLAST
Propeptidei252 – 26918
PRO_0000008133Add
BLAST
Peptidei270 – 29021Sarafotoxin-E
PRO_0000008134Add
BLAST
Propeptidei292 – 30918
PRO_0000008135Add
BLAST
Peptidei310 – 33021Sarafotoxin-A, Ser-isoform
PRO_0000008136Add
BLAST
Propeptidei332 – 34918
PRO_0000008137Add
BLAST
Peptidei350 – 37021Sarafotoxin-C
PRO_0000008138Add
BLAST
Propeptidei372 – 38918
PRO_0000008139Add
BLAST
Peptidei390 – 41021Sarafotoxin-C
PRO_0000008140Add
BLAST
Propeptidei412 – 42918
PRO_0000008141Add
BLAST
Peptidei430 – 45021Sarafotoxin-B
PRO_0000008142Add
BLAST
Propeptidei452 – 46918
PRO_0000008143Add
BLAST
Peptidei470 – 49021Sarafotoxin-A, Ser-isoform
PRO_0000008144Add
BLAST
Propeptidei492 – 50918
PRO_0000008145Add
BLAST
Peptidei510 – 53021Sarafotoxin-A, Thr-isoform
PRO_0000008146Add
BLAST
Propeptidei532 – 54312
PRO_0000008147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 84 By similarity
Disulfide bondi72 ↔ 80 By similarity
Disulfide bondi110 ↔ 124 By similarity
Disulfide bondi112 ↔ 120 By similarity
Disulfide bondi150 ↔ 1641 Publication
Disulfide bondi152 ↔ 1601 Publication
Disulfide bondi190 ↔ 204 By similarity
Disulfide bondi192 ↔ 200 By similarity
Disulfide bondi230 ↔ 244 By similarity
Disulfide bondi232 ↔ 240 By similarity
Disulfide bondi270 ↔ 284 By similarity
Disulfide bondi272 ↔ 280 By similarity
Disulfide bondi310 ↔ 324 By similarity
Disulfide bondi312 ↔ 320 By similarity
Disulfide bondi350 ↔ 364 By similarity
Disulfide bondi352 ↔ 360 By similarity
Disulfide bondi390 ↔ 404 By similarity
Disulfide bondi392 ↔ 400 By similarity
Disulfide bondi430 ↔ 4441 Publication
Disulfide bondi432 ↔ 4401 Publication
Disulfide bondi470 ↔ 484 By similarity
Disulfide bondi472 ↔ 480 By similarity
Disulfide bondi510 ↔ 524 By similarity
Disulfide bondi512 ↔ 520 By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi433 – 4353
Helixi438 – 4447

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SRBNMR-A430-450[»]
ProteinModelPortaliP13208.

Miscellaneous databases

EvolutionaryTraceiP13208.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati51 – 90401
Add
BLAST
Repeati91 – 130402
Add
BLAST
Repeati131 – 170403
Add
BLAST
Repeati171 – 210404
Add
BLAST
Repeati211 – 250405
Add
BLAST
Repeati251 – 290406
Add
BLAST
Repeati291 – 330407
Add
BLAST
Repeati331 – 370408
Add
BLAST
Repeati371 – 410409
Add
BLAST
Repeati411 – 4504010
Add
BLAST
Repeati451 – 4904011
Add
BLAST
Repeati491 – 5304012
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 53048012 X 40 AA tandem repeats
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG017852.

Family and domain databases

InterProiIPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 12 hits.
[Graphical view]
SMARTiSM00272. END. 12 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 12 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13208-1 [UniParc]FASTAAdd to Basket

« Hide

MALLPRLAAG GLLLLLALAA LEGKPAPSAL SQLLEKRSED QAAAGRIIDG    50
GDTKQAARDP SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP 100
SPQRNVEPLC TCNDMTDEEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC 150
SCKDMTDKEC LYFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC 200
LNFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC LNFCHQDVIW 250
RDTKQAARDP SPQRNVEPLC TCKDMTDKEC LYFCHQGIIW RDTKQAARDP 300
SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC 350
TCNDMTDEEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC 400
LNFCHQDVIW RDTKQAARDP SPQRNVEPLC SCKDMTDKEC LYFCHQDVIW 450
RDTKQAARDP SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP 500
SPQRNVEPLC SCKDMTDKEC LNFCHQDVIW KNADTSANPE FLG 543
Length:543
Mass (Da):62,326
Last modified:February 1, 1994 - v2
Checksum:iDF84A9631392FE4A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07528 mRNA. Translation: AAA48515.1.
D13322 Genomic DNA. Translation: BAA02579.2.
PIRiA46601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07528 mRNA. Translation: AAA48515.1 .
D13322 Genomic DNA. Translation: BAA02579.2 .
PIRi A46601.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SRB NMR - A 430-450 [» ]
ProteinModelPortali P13208.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG017852.

Miscellaneous databases

EvolutionaryTracei P13208.

Family and domain databases

InterProi IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view ]
Pfami PF00322. Endothelin. 12 hits.
[Graphical view ]
SMARTi SM00272. END. 12 hits.
[Graphical view ]
PROSITEi PS00270. ENDOTHELIN. 12 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of cDNAs encoding precursors of sarafotoxins. Evidence for an unusual 'rosary-type' organization."
    Ducancel F., Matre V., Dupont C., Lajeunesse E., Wollberg Z., Bdolah A., Kochva E., Boulain J.-C., Menez A.
    J. Biol. Chem. 268:3052-3055(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  2. "Sarafotoxins S6: several isotoxins from Atractaspis engaddensis (burrowing asp) venom that affect the heart."
    Takasaki C., Tamiya N., Bdolah A., Wollberg Z., Kochva E.
    Toxicon 26:543-548(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE (SARAFOTOXINS A; B AND C), LETHAL DOSE.
    Tissue: Venom.
  3. "Sarafotoxin, a novel vasoconstrictor peptide: phosphoinositide hydrolysis in rat heart and brain."
    Kloog Y., Ambar I., Sokolovsky M., Kochva E., Wollberg Z., Bdolah A.
    Science 242:268-270(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE (SARAFOTOXINS A; B AND C).
    Tissue: Venom.
  4. "Cloning and sequence analysis of a snake, Atractaspis engaddensis gene encoding sarafotoxin S6c."
    Takasaki C., Itoh Y., Onda H., Fujino M.
    Biochem. Biophys. Res. Commun. 189:1527-1533(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 108-130.
    Tissue: Liver.
  5. "Studies on the disulfide bridges of sarafotoxins. Chemical synthesis of sarafotoxin S6B and its homologue with different disulfide bridges."
    Aimoto S., Hojoh H., Takasaki C.
    Biochem. Int. 21:1051-1057(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS, SYNTHESIS OF SARAFOTOXIN B.
  6. "Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: role of the C-terminus extension."
    Mourier G., Hajj M., Cordier F., Zorba A., Gao X., Coskun T., Herbet A., Marcon E., Beau F., Delepierre M., Ducancel F., Servent D.
    Biochimie 94:461-470(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOXIN TARGET, MUTAGENESIS OF TRP-170 AND TRP-450.
  7. "Conformation of sarafotoxin-6b in aqueous solution determined by NMR spectroscopy and distance geometry."
    Mills R.G., Atkins A.R., Harvey T., Junius F.K., Smith R., King G.F.
    FEBS Lett. 282:247-252(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF SARAFOTOXIN B.
  8. "1H NMR study of the solution structure of sarafotoxin-S6b."
    Aumelas A., Chiche L., Mahe E., Le-Nguyen D., Sizun P., Berthault P., Perly B.
    Neurochem. Int. 18:471-475(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF SARAFOTOXIN B.
  9. "1H NMR studies of sarafotoxin SRTb, a nonselective endothelin receptor agonist, and IRL 1620, an ETB receptor-specific agonist."
    Atkins A.R., Martin R.C., Smith R.
    Biochemistry 34:2026-2033(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF SARAFOTOXIN B.

Entry informationi

Entry nameiSRTX_ATREN
AccessioniPrimary (citable) accession number: P13208
Secondary accession number(s): P13209, P13210
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3