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P13208

- SRTX_ATREN

UniProt

P13208 - SRTX_ATREN

Protein

Sarafotoxin

Gene
N/A
Organism
Atractaspis engaddensis (Israeli burrowing asp) (Israeli mole viper)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm.1 Publication
    Sarafotoxin-B: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm By similarity. Displays high agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the picomolar range) and endothelin-1 receptor (EDNRA) (lower affinities) (PubMed:21889567).By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei90 – 901Endothelin-receptor binding siteBy similarity
    Sitei130 – 1301Endothelin-receptor binding siteBy similarity
    Sitei170 – 1701Endothelin-receptor binding siteBy similarity
    Sitei210 – 2101Endothelin-receptor binding siteBy similarity
    Sitei250 – 2501Endothelin-receptor binding siteBy similarity
    Sitei290 – 2901Endothelin-receptor binding siteBy similarity
    Sitei330 – 3301Endothelin-receptor binding siteBy similarity
    Sitei370 – 3701Endothelin-receptor binding siteBy similarity
    Sitei410 – 4101Endothelin-receptor binding siteBy similarity
    Sitei450 – 4501Endothelin-receptor binding siteBy similarity
    Sitei490 – 4901Endothelin-receptor binding siteBy similarity
    Sitei530 – 5301Endothelin-receptor binding siteBy similarity

    GO - Biological processi

    1. regulation of vasoconstriction Source: InterPro
    2. vasoconstriction Source: UniProtKB-KW

    Keywords - Molecular functioni

    Cardiotoxin, G-protein coupled receptor impairing toxin, Toxin, Vasoactive, Vasoconstrictor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sarafotoxin
    Cleaved into the following 5 chains:
    Sarafotoxin-A, Ser-isoform
    Short name:
    SRTX-A
    Short name:
    Sarafotoxin-A
    Alternative name(s):
    S6A
    Sarafotoxin-C
    Short name:
    SRTX-C
    Alternative name(s):
    S6C
    Sarafotoxin-B
    Short name:
    SRTX-B
    Alternative name(s):
    S6B
    Sarafotoxin-E
    Short name:
    SRTX-E
    Alternative name(s):
    S6E
    OrganismiAtractaspis engaddensis (Israeli burrowing asp) (Israeli mole viper)
    Taxonomic identifieri8600 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaAtractaspididaeAtractaspis

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Toxic dosei

    Sarafotoxin-A and sarafotoxin-B: LD50 is 0.015 mg/kg by intravenous injection into mice.2 Publications
    Sarafotoxin-C: LD50 is 0.3 mg/kg by intravenous injection into mice.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi170 – 1701W → WVNRN or WDEP: Drastic decrease in affinity for ET-B receptors (3 and 5-orders or magnitude for VNRN and DEP, respectively). 1 Publication
    Mutagenesisi450 – 4501W → WVNRN or DEP: Drastic decrease in affinity for ET-B receptors (3 and 5-orders or magnitude for VNRN and DEP, respectively). 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Propeptidei24 – 6946PRO_0000008123Add
    BLAST
    Peptidei70 – 9021Sarafotoxin-A, Ser-isoformPRO_0000008124Add
    BLAST
    Propeptidei92 – 10918PRO_0000008125Add
    BLAST
    Peptidei110 – 13021Sarafotoxin-CPRO_0000008126Add
    BLAST
    Propeptidei132 – 14918PRO_0000008127Add
    BLAST
    Peptidei150 – 17021Sarafotoxin-BPRO_0000008128Add
    BLAST
    Propeptidei172 – 18918PRO_0000008129Add
    BLAST
    Peptidei190 – 21021Sarafotoxin-CPRO_0000008130Add
    BLAST
    Propeptidei212 – 22918PRO_0000008131Add
    BLAST
    Peptidei230 – 25021Sarafotoxin-CPRO_0000008132Add
    BLAST
    Propeptidei252 – 26918PRO_0000008133Add
    BLAST
    Peptidei270 – 29021Sarafotoxin-EPRO_0000008134Add
    BLAST
    Propeptidei292 – 30918PRO_0000008135Add
    BLAST
    Peptidei310 – 33021Sarafotoxin-A, Ser-isoformPRO_0000008136Add
    BLAST
    Propeptidei332 – 34918PRO_0000008137Add
    BLAST
    Peptidei350 – 37021Sarafotoxin-CPRO_0000008138Add
    BLAST
    Propeptidei372 – 38918PRO_0000008139Add
    BLAST
    Peptidei390 – 41021Sarafotoxin-CPRO_0000008140Add
    BLAST
    Propeptidei412 – 42918PRO_0000008141Add
    BLAST
    Peptidei430 – 45021Sarafotoxin-BPRO_0000008142Add
    BLAST
    Propeptidei452 – 46918PRO_0000008143Add
    BLAST
    Peptidei470 – 49021Sarafotoxin-A, Ser-isoformPRO_0000008144Add
    BLAST
    Propeptidei492 – 50918PRO_0000008145Add
    BLAST
    Peptidei510 – 53021Sarafotoxin-A, Thr-isoformPRO_0000008146Add
    BLAST
    Propeptidei532 – 54312PRO_0000008147Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi70 ↔ 84By similarity
    Disulfide bondi72 ↔ 80By similarity
    Disulfide bondi110 ↔ 124By similarity
    Disulfide bondi112 ↔ 120By similarity
    Disulfide bondi150 ↔ 1641 Publication
    Disulfide bondi152 ↔ 1601 Publication
    Disulfide bondi190 ↔ 204By similarity
    Disulfide bondi192 ↔ 200By similarity
    Disulfide bondi230 ↔ 244By similarity
    Disulfide bondi232 ↔ 240By similarity
    Disulfide bondi270 ↔ 284By similarity
    Disulfide bondi272 ↔ 280By similarity
    Disulfide bondi310 ↔ 324By similarity
    Disulfide bondi312 ↔ 320By similarity
    Disulfide bondi350 ↔ 364By similarity
    Disulfide bondi352 ↔ 360By similarity
    Disulfide bondi390 ↔ 404By similarity
    Disulfide bondi392 ↔ 400By similarity
    Disulfide bondi430 ↔ 4441 Publication
    Disulfide bondi432 ↔ 4401 Publication
    Disulfide bondi470 ↔ 484By similarity
    Disulfide bondi472 ↔ 480By similarity
    Disulfide bondi510 ↔ 524By similarity
    Disulfide bondi512 ↔ 520By similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    543
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi433 – 4353
    Helixi438 – 4447

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SRBNMR-A430-450[»]
    ProteinModelPortaliP13208.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13208.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati51 – 90401Add
    BLAST
    Repeati91 – 130402Add
    BLAST
    Repeati131 – 170403Add
    BLAST
    Repeati171 – 210404Add
    BLAST
    Repeati211 – 250405Add
    BLAST
    Repeati251 – 290406Add
    BLAST
    Repeati291 – 330407Add
    BLAST
    Repeati331 – 370408Add
    BLAST
    Repeati371 – 410409Add
    BLAST
    Repeati411 – 4504010Add
    BLAST
    Repeati451 – 4904011Add
    BLAST
    Repeati491 – 5304012Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 53048012 X 40 AA tandem repeatsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the endothelin/sarafotoxin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOVERGENiHBG017852.

    Family and domain databases

    InterProiIPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    [Graphical view]
    PfamiPF00322. Endothelin. 12 hits.
    [Graphical view]
    SMARTiSM00272. END. 12 hits.
    [Graphical view]
    PROSITEiPS00270. ENDOTHELIN. 12 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13208-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLPRLAAG GLLLLLALAA LEGKPAPSAL SQLLEKRSED QAAAGRIIDG    50
    GDTKQAARDP SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP 100
    SPQRNVEPLC TCNDMTDEEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC 150
    SCKDMTDKEC LYFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC 200
    LNFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC LNFCHQDVIW 250
    RDTKQAARDP SPQRNVEPLC TCKDMTDKEC LYFCHQGIIW RDTKQAARDP 300
    SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC 350
    TCNDMTDEEC LNFCHQDVIW RDTKQAARDP SPQRNVEPLC TCNDMTDEEC 400
    LNFCHQDVIW RDTKQAARDP SPQRNVEPLC SCKDMTDKEC LYFCHQDVIW 450
    RDTKQAARDP SPQRNVEPLC SCKDMSDKEC LNFCHQDVIW RDTKQAARDP 500
    SPQRNVEPLC SCKDMTDKEC LNFCHQDVIW KNADTSANPE FLG 543
    Length:543
    Mass (Da):62,326
    Last modified:February 1, 1994 - v2
    Checksum:iDF84A9631392FE4A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07528 mRNA. Translation: AAA48515.1.
    D13322 Genomic DNA. Translation: BAA02579.2.
    PIRiA46601.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07528 mRNA. Translation: AAA48515.1 .
    D13322 Genomic DNA. Translation: BAA02579.2 .
    PIRi A46601.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SRB NMR - A 430-450 [» ]
    ProteinModelPortali P13208.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG017852.

    Miscellaneous databases

    EvolutionaryTracei P13208.

    Family and domain databases

    InterProi IPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    [Graphical view ]
    Pfami PF00322. Endothelin. 12 hits.
    [Graphical view ]
    SMARTi SM00272. END. 12 hits.
    [Graphical view ]
    PROSITEi PS00270. ENDOTHELIN. 12 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of cDNAs encoding precursors of sarafotoxins. Evidence for an unusual 'rosary-type' organization."
      Ducancel F., Matre V., Dupont C., Lajeunesse E., Wollberg Z., Bdolah A., Kochva E., Boulain J.-C., Menez A.
      J. Biol. Chem. 268:3052-3055(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Venom gland.
    2. "Sarafotoxins S6: several isotoxins from Atractaspis engaddensis (burrowing asp) venom that affect the heart."
      Takasaki C., Tamiya N., Bdolah A., Wollberg Z., Kochva E.
      Toxicon 26:543-548(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE (SARAFOTOXINS A; B AND C), LETHAL DOSE.
      Tissue: Venom.
    3. "Sarafotoxin, a novel vasoconstrictor peptide: phosphoinositide hydrolysis in rat heart and brain."
      Kloog Y., Ambar I., Sokolovsky M., Kochva E., Wollberg Z., Bdolah A.
      Science 242:268-270(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE (SARAFOTOXINS A; B AND C).
      Tissue: Venom.
    4. "Cloning and sequence analysis of a snake, Atractaspis engaddensis gene encoding sarafotoxin S6c."
      Takasaki C., Itoh Y., Onda H., Fujino M.
      Biochem. Biophys. Res. Commun. 189:1527-1533(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 108-130.
      Tissue: Liver.
    5. "Studies on the disulfide bridges of sarafotoxins. Chemical synthesis of sarafotoxin S6B and its homologue with different disulfide bridges."
      Aimoto S., Hojoh H., Takasaki C.
      Biochem. Int. 21:1051-1057(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS, SYNTHESIS OF SARAFOTOXIN B.
    6. "Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: role of the C-terminus extension."
      Mourier G., Hajj M., Cordier F., Zorba A., Gao X., Coskun T., Herbet A., Marcon E., Beau F., Delepierre M., Ducancel F., Servent D.
      Biochimie 94:461-470(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TOXIN TARGET, MUTAGENESIS OF TRP-170 AND TRP-450.
    7. "Conformation of sarafotoxin-6b in aqueous solution determined by NMR spectroscopy and distance geometry."
      Mills R.G., Atkins A.R., Harvey T., Junius F.K., Smith R., King G.F.
      FEBS Lett. 282:247-252(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF SARAFOTOXIN B.
    8. "1H NMR study of the solution structure of sarafotoxin-S6b."
      Aumelas A., Chiche L., Mahe E., Le-Nguyen D., Sizun P., Berthault P., Perly B.
      Neurochem. Int. 18:471-475(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF SARAFOTOXIN B.
    9. "1H NMR studies of sarafotoxin SRTb, a nonselective endothelin receptor agonist, and IRL 1620, an ETB receptor-specific agonist."
      Atkins A.R., Martin R.C., Smith R.
      Biochemistry 34:2026-2033(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF SARAFOTOXIN B.

    Entry informationi

    Entry nameiSRTX_ATREN
    AccessioniPrimary (citable) accession number: P13208
    Secondary accession number(s): P13209, P13210
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3