P13207 (EDN3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endothelin-3
Short name=ET-3

Alternative name(s):
Preproendothelin-3
Short name=PPET3

Gene names

Name:

Edn3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Vasoactive Vasoconstrictor
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of hormone secretion Inferred from direct assay PubMed 1533364. Source: RGD regulation of neurotransmitter secretion Inferred from direct assay PubMed 12184995. Source: RGD regulation of vasoconstriction Traceable author statement PubMed 12184995. Source: RGD vasoconstriction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	bombesin receptor binding Traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 50	31			PRO_0000008120
	Peptide	53 – 73	21	Endothelin-3		PRO_0000008121
	Propeptide	74 – 167	94			PRO_0000008122
Regions
	Region	115 – 135	21	Endothelin-like
Sites
	Site	73 – 74	2	Cleavage; by KEL By similarity
Amino acid modifications
	Disulfide bond	53 ↔ 67		By similarity
	Disulfide bond	55 ↔ 63		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13207 [UniParc]. Last modified July 1, 1993. Version 3. Checksum: B219DA1D7C375DC2	FASTA	167	18,453
	10 20 30 40 50 60 MELGLWLLLG LTVTSAAAAL PAQPGNAGQE RGPGRSGDQE EKRVPAHHRP RRCTCFTYKD 70 80 90 100 110 120 KECVYYCHLD IIWINTPEQT VPYGLSNHRG SLRGKRSSGP VPESSQSSPQ TRLRCACSGV 130 140 150 160 DDKACAYFCA HVTSYSRRAE KAAAEEKQET GGPRQRLKSR TDKVHQP

References

[1]	"Cloning and expression of rat preproendothelin-3 cDNA." Shiba R., Sakurai T., Yamada G., Morimoto H., Saito A., Masaki T., Goto K. Biochem. Biophys. Res. Commun. 186:588-594(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Primary structure, synthesis, and biological activity of rat endothelin, an endothelium-derived vasoconstrictor peptide." Yanagisawa M., Inoue A., Ishikawa T., Kasuya Y., Kimura S., Kumagaye S., Nakajima K., Watanabe T.X., Sakakibara S., Goto K., Masaki T. Proc. Natl. Acad. Sci. U.S.A. 85:6964-6967(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-77.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S39779 mRNA. Translation: AAB22502.1. J04075 Genomic DNA. No translation available.
IPI	IPI00199301.
PIR	JC1102.
RefSeq	NP_001071118.1. NM_001077650.1.
UniGene	Rn.201692.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000009826.
Proteomic databases
PRIDE	P13207.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	366270.
KEGG	rno:366270.
UCSC	RGD:2534. rat.
Organism-specific databases
CTD	1908.
RGD	2534. Edn3.
Phylogenomic databases
eggNOG	NOG41573.
HOGENOM	HOG000231110.
HOVERGEN	HBG051442.
KO	K05227.
OrthoDB	EOG4Z62PG.
Gene expression databases
ArrayExpress	P13207.
Genevestigator	P13207.
GermOnline	ENSRNOG00000007477. Rattus norvegicus.
Family and domain databases
InterPro	IPR020475. Bibrotoxin/Sarafotoxin-D. IPR019764. Endothelin_toxin_CS. IPR001928. Endothln-like_toxin. [Graphical view]
Pfam	PF00322. Endothelin. 1 hit. [Graphical view]
PRINTS	PR00365. ENDOTHELIN.
SMART	SM00272. END. 2 hits. [Graphical view]
PROSITE	PS00270. ENDOTHELIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	689095.

Entry information

Entry name

EDN3_RAT

Accession

Primary (citable) accession number: P13207

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program