P13206 (EDN1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endothelin-1
Short name=ET-1

Alternative name(s):
Preproendothelin-1
Short name=PPET1

Gene names

Name:

EDN1

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Vasoactive Vasoconstrictor
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	body fluid secretion Inferred from electronic annotation. Source: Compara calcium-mediated signaling Inferred from electronic annotation. Source: Compara cartilage development Inferred from electronic annotation. Source: Compara dorsal/ventral pattern formation Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Inferred from electronic annotation. Source: Compara glucose transport Inferred from electronic annotation. Source: Compara heart development Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from electronic annotation. Source: Compara inositol phosphate-mediated signaling Inferred from electronic annotation. Source: Compara middle ear morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of cellular protein metabolic process Inferred from electronic annotation. Source: Compara negative regulation of nitric-oxide synthase biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara neural crest cell development Inferred from electronic annotation. Source: Compara nitric oxide transport Inferred from electronic annotation. Source: Compara patterning of blood vessels Inferred from electronic annotation. Source: Compara peptide hormone secretion Inferred from electronic annotation. Source: Compara phosphatidylinositol 3-kinase cascade Inferred from electronic annotation. Source: Compara phospholipase D-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of JUN kinase activity Inferred from electronic annotation. Source: Compara positive regulation of cardiac muscle hypertrophy Inferred from electronic annotation. Source: Compara positive regulation of cell migration Inferred from electronic annotation. Source: Compara positive regulation of cell size Inferred from electronic annotation. Source: Compara positive regulation of heart rate Inferred from electronic annotation. Source: Compara positive regulation of hormone secretion Inferred from electronic annotation. Source: Compara positive regulation of mitosis Inferred from electronic annotation. Source: Compara positive regulation of prostaglandin-endoperoxide synthase activity Inferred from electronic annotation. Source: Compara positive regulation of receptor biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of renal sodium excretion Inferred from electronic annotation. Source: Compara positive regulation of sarcomere organization Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of urine volume Inferred from electronic annotation. Source: Compara prostaglandin biosynthetic process Inferred from electronic annotation. Source: Compara protein kinase C deactivation Inferred from electronic annotation. Source: Compara protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of pH Inferred from electronic annotation. Source: Compara regulation of systemic arterial blood pressure by endothelin Inferred from electronic annotation. Source: Compara regulation of vasoconstriction Inferred from electronic annotation. Source: InterPro respiratory gaseous exchange Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara rhythmic excitation Inferred from electronic annotation. Source: Compara vein smooth muscle contraction Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 50	25			PRO_0000008049
	Peptide	53 – 73	21	Endothelin-1		PRO_0000008050
	Propeptide	74 – 202	129			PRO_0000008051
Regions
	Region	110 – 124	15	Endothelin-like
Sites
	Site	73 – 74	2	Cleavage; by KEL By similarity
Amino acid modifications
	Disulfide bond	53 ↔ 67		By similarity
	Disulfide bond	55 ↔ 63		By similarity
Experimental info
	Sequence conflict	122	1	T → N in AAK01932. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P13206 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 2EE08049230C2FD0	FASTA	202	22,977
	10 20 30 40 50 60 MDYFPMIFSL LFVAFQGAPE AAVLGAELST GPDSRGEKPA PSAPWRPRRS KRCSCSSLMD 70 80 90 100 110 120 KECVYFCHLD IIWVNTPEHI VPYGLGSPSR SKRSLKDLFI TKATDHRKRC QCASQKDKKC 130 140 150 160 170 180 WTFCQAGKEL REQDSMEKGW NGHKKGKDCS ELGEKSKQQQ LVAGRKIRRL DAISNSIKTA 190 200 FRVAKLKAEL YRENKVTHNR TH

References

[1]	"Cloning of canine preproendothelin-1 cDNA." Uchide T. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and sequencing of a canine gene segment encoding mature endothelin." Kimura C., Itoh Y., Ohkubo S., Ogi K., Onda H., Fujino M. Nucleic Acids Res. 17:3290-3290(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 23-78. Tissue: Liver.
[3]	"Canis familiaris endothelin-1 and big endothelin-1 cDNA." Biondo A.W., Wiedmeyer C.E., Solter P.F., Sisson D.D. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 27-122. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB115087 mRNA. Translation: BAD02920.1. X14610 Genomic DNA. No translation available. AF333433 mRNA. Translation: AAK01932.1.
PIR	S04074.
RefSeq	NP_001002956.1. NM_001002956.1.
UniGene	Cfa.125.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000015551; ENSCAFP00000014389; ENSCAFG00000009794.
GeneID	403424.
KEGG	cfa:403424.
Organism-specific databases
CTD	1906.
Phylogenomic databases
eggNOG	NOG46593.
GeneTree	ENSGT00530000063310.
HOGENOM	HOG000231110.
HOVERGEN	HBG051441.
KO	K16366.
OMA	RCQCASQ.
OrthoDB	EOG4QZ7N8.
Family and domain databases
InterPro	IPR020475. Bibrotoxin/Sarafotoxin-D. IPR019764. Endothelin_toxin_CS. IPR001928. Endothln-like_toxin. [Graphical view]
Pfam	PF00322. Endothelin. 1 hit. [Graphical view]
PRINTS	PR00365. ENDOTHELIN.
SMART	SM00272. END. 2 hits. [Graphical view]
PROSITE	PS00270. ENDOTHELIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816944.

Entry information

Entry name

EDN1_CANFA

Accession

Primary (citable) accession number: P13206
Secondary accession number(s): Q765Z3, Q9BDC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	July 19, 2004
Last modified:	April 3, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program