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Protein

Natriuretic peptides B

Gene

Nppb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity).By similarity

GO - Molecular functioni

  • hormone activity Source: RGD
  • peptide hormone receptor binding Source: RGD

GO - Biological processi

  • cell growth involved in cardiac muscle cell development Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • cGMP biosynthetic process Source: RGD
  • heart development Source: RGD
  • inflammatory response Source: RGD
  • negative regulation of cell growth Source: RGD
  • negative regulation of smooth muscle cell proliferation Source: RGD
  • negative regulation of systemic arterial blood pressure Source: RGD
  • positive regulation of collecting lymphatic vessel constriction Source: RGD
  • positive regulation of renal sodium excretion Source: RGD
  • positive regulation of urine volume Source: RGD
  • receptor guanylyl cyclase signaling pathway Source: UniProtKB
  • regulation of blood vessel size Source: UniProtKB-KW
  • response to calcium ion Source: RGD
  • response to drug Source: RGD
  • response to hypoxia Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to peptide hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hormone, Vasoactive

Names & Taxonomyi

Protein namesi
Recommended name:
Natriuretic peptides B
Alternative name(s):
Gamma-brain natriuretic peptide
Iso-ANP
Cleaved into the following chain:
Alternative name(s):
5 kDa cardiac natriuretic peptide
BNP-34
Gene namesi
Name:Nppb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi3194. Nppb.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: RGD
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Peptidei27 – 12195Natriuretic peptides BPRO_0000001538Add
BLAST
Peptidei77 – 12145Brain natriuretic peptide 45PRO_0000001539Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi99 ↔ 115

Post-translational modificationi

The brain natriuretic peptide 32 form is cleaved at Ser-91 by the prolyl endopeptidase FAP (seprase) activity (in vitro).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei91 – 922Cleavage; by FAPBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP13205.
PRIDEiP13205.

Expressioni

Tissue specificityi

Brain and also in atria, but at much lower levels than ANP.

Gene expression databases

GenevisibleiP13205. RN.

Interactioni

GO - Molecular functioni

  • hormone activity Source: RGD
  • peptide hormone receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010778.

Family & Domainsi

Sequence similaritiesi

Belongs to the natriuretic peptide family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J2F8. Eukaryota.
ENOG410ZE1B. LUCA.
GeneTreeiENSGT00730000112870.
HOGENOMiHOG000033937.
HOVERGENiHBG004227.
InParanoidiP13205.
KOiK12335.
OMAiVTHISSC.
OrthoDBiEOG7P2XV4.
PhylomeDBiP13205.
TreeFamiTF106304.

Family and domain databases

InterProiIPR000663. Natr_peptide.
IPR030480. Natr_peptide_CS.
IPR002408. Natriuretic_peptide_brain.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00712. BNATPEPTIDE.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLQKVLPQM ILLLLFLNLS PLGGHSHPLG SPSQSPEQST MQKLLELIRE
60 70 80 90 100
KSEEMAQRQL SKDQGPTKEL LKRVLRSQDS AFRIQERLRN SKMAHSSSCF
110 120
GQKIDRIGAV SRLGCDGLRL F
Length:121
Mass (Da):13,656
Last modified:February 1, 1991 - v3
Checksum:iB5D4151ED18C7294
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151L → V in AAA41456 (PubMed:2144113).Curated
Sequence conflicti120 – 1201L → Q AA sequence (PubMed:2525380).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25297 mRNA. Translation: AAA57269.1.
M60731 Genomic DNA. Translation: AAA41456.1.
M60266 Genomic DNA. Translation: AAA41455.1.
PIRiA30162.
RefSeqiNP_113733.1. NM_031545.1.
UniGeneiRn.3835.

Genome annotation databases

EnsembliENSRNOT00000010779; ENSRNOP00000010778; ENSRNOG00000008141.
GeneIDi25105.
KEGGirno:25105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25297 mRNA. Translation: AAA57269.1.
M60731 Genomic DNA. Translation: AAA41456.1.
M60266 Genomic DNA. Translation: AAA41455.1.
PIRiA30162.
RefSeqiNP_113733.1. NM_031545.1.
UniGeneiRn.3835.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010778.

Proteomic databases

PaxDbiP13205.
PRIDEiP13205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010779; ENSRNOP00000010778; ENSRNOG00000008141.
GeneIDi25105.
KEGGirno:25105.

Organism-specific databases

CTDi4879.
RGDi3194. Nppb.

Phylogenomic databases

eggNOGiENOG410J2F8. Eukaryota.
ENOG410ZE1B. LUCA.
GeneTreeiENSGT00730000112870.
HOGENOMiHOG000033937.
HOVERGENiHBG004227.
InParanoidiP13205.
KOiK12335.
OMAiVTHISSC.
OrthoDBiEOG7P2XV4.
PhylomeDBiP13205.
TreeFamiTF106304.

Miscellaneous databases

PROiP13205.

Gene expression databases

GenevisibleiP13205. RN.

Family and domain databases

InterProiIPR000663. Natr_peptide.
IPR030480. Natr_peptide_CS.
IPR002408. Natriuretic_peptide_brain.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00712. BNATPEPTIDE.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of cDNA encoding a precursor for rat brain natriuretic peptide."
    Kojima M., Minamino N., Kangawa K., Matsuo H.
    Biochem. Biophys. Res. Commun. 159:1420-1426(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organization of the gene for iso-rANP, a rat B-type natriuretic peptide."
    Roy R.N., Flynn T.G.
    Biochem. Biophys. Res. Commun. 171:416-423(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Differential expression of natriuretic peptide genes in cardiac and extracardiac tissues."
    Dagnino L., Drouin J., Nemer M.
    Mol. Endocrinol. 5:1292-1300(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Isolation and identification of rat brain natriuretic peptides in cardiac atrium."
    Abuyara M., Hino J., Minamino N., Kangawa K., Matsuo H.
    Biochem. Biophys. Res. Commun. 163:226-232(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-121.
  5. Cited for: PROTEIN SEQUENCE OF 77-121.
    Tissue: Heart.
  6. "Isolation and characterization of iso-rANP, a new natriuretic peptide from rat atria."
    Flynn T.G., Brar A., Tremblay L., Sarda I., Lyons C., Jennings D.B.
    Biochem. Biophys. Res. Commun. 161:830-837(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 77-121.
  7. Cited for: PROTEIN SEQUENCE OF 99-115.

Entry informationi

Entry nameiANFB_RAT
AccessioniPrimary (citable) accession number: P13205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1991
Last modified: June 8, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.