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Protein

mRNA export factor ICP27 homolog

Gene

EJRF1

Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably acts as a viral splicing factor that regulates viral RNA splicing. Functions as a multifunctional regulator of the expression of viral lytic genes (By similarity). Early protein that promotes the accumulation and nuclear export of viral intronless RNA transcripts by interacting with mRNAs and cellular export proteins.By similarity1 Publication

Miscellaneous

ORF50 and ORF57 are the earliest genes expressed in the lytic cycle.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi295ZincBy similarity1
Metal bindingi385ZincBy similarity1
Metal bindingi389ZincBy similarity1
Metal bindingi394ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri295 – 394CHC2-typeBy similarityAdd BLAST100

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, RNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA export factor ICP27 homolog
Alternative name(s):
52 kDa immediate-early phosphoprotein
EB2 protein homolog
Gene namesi
Name:EJRF1
ORF Names:ORF57
OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10383 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
Proteomesi
  • UP000000587 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001158321 – 417mRNA export factor ICP27 homologAdd BLAST417

Expressioni

Inductioni

Transactivated by ORF50 protein.

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer. Homodimerization is required for transactivation (By similarity). Interacts with host ALYREF and with mouse ALYREF2. Associates in a complex with RNA, and host export factors NXF1/TAP and ALYREF or ALYREF2; these interactions allow nuclear export of viral transcripts.By similarity2 Publications

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi108 – 118Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YKANMR-B103-120[»]
SMRiP13199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 120Interaction with RNAAdd BLAST57
Regioni106 – 120Interaction with host ALYREF or mouse ALYREF2Add BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 94Nuclear localization signalSequence analysis7
Motifi118 – 127Nuclear localization signalSequence analysis10

Domaini

Binds viral intronless RNAs; the RNA binding site overlaps partially with the binding site for host ALYREF or ALYREF2.

Sequence similaritiesi

Belongs to the HHV-1 ICP27 protein family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri295 – 394CHC2-typeBy similarityAdd BLAST100

Keywords - Domaini

Zinc-finger

Phylogenomic databases

OrthoDBiVOG090000AK.

Family and domain databases

InterProiView protein in InterPro
IPR008648. ICP27-like.
PfamiView protein in Pfam
PF05459. Herpes_UL69. 1 hit.

Sequencei

Sequence statusi: Complete.

P13199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDIIEGGIS SDDDFDSSDS SSDEEESDTS PQIMKSDVTM ASPPSTPEPS
60 70 80 90 100
PDVSASTSNL KRERQRSPIT WEHQSPLSRV YRSPSPMRFG KRPRISSNST
110 120 130 140 150
SRSCKTSWAD RVREAAAQRR PSRPFRKPYS HPRNGPLRNG PPRAPPLLKL
160 170 180 190 200
FDISILPKSG EPKLFLPVPS LPCQEAEKTN DKYVLAMAQR AMHDVPISSK
210 220 230 240 250
QLTANLLPVK FKPLLSIVRY TPNYYYWVSM RKETIASANL CTVAAFLDES
260 270 280 290 300
LCWGQQYLKN DFIFSENGKD IILDTSSALL SQLVHKIKML PFCHCLMQTT
310 320 330 340 350
PQDHIVKQVC YLIASNNRIL DAVRYLQTSV IKSPIVLLLA YAVCLPAAII
360 370 380 390 400
CTKNETQLYS HCMRILKEYR PGDVMNILHE SLTQHLNKCP SSTCAYTTRA
410
IVGTKANTTG LFFLPTQ
Length:417
Mass (Da):46,816
Last modified:April 1, 1993 - v2
Checksum:i12F0EA3733F00940
GO

Sequence cautioni

The sequence AAA46125 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA. Translation: CAA45680.1.
M86409 Genomic DNA. Translation: AAA46125.1. Different initiation.
M21943 Genomic DNA. Translation: AAA66558.1.
RefSeqiNP_040259.1. NC_001350.1.

Genome annotation databases

GeneIDi1682520.
KEGGivg:1682520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA. Translation: CAA45680.1.
M86409 Genomic DNA. Translation: AAA46125.1. Different initiation.
M21943 Genomic DNA. Translation: AAA66558.1.
RefSeqiNP_040259.1. NC_001350.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YKANMR-B103-120[»]
SMRiP13199.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1682520.
KEGGivg:1682520.

Phylogenomic databases

OrthoDBiVOG090000AK.

Family and domain databases

InterProiView protein in InterPro
IPR008648. ICP27-like.
PfamiView protein in Pfam
PF05459. Herpes_UL69. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiICP27_SHV21
AccessioniPrimary (citable) accession number: P13199
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 1, 1993
Last modified: April 12, 2017
This is version 67 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.