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P13195

- HEM1_RAT

UniProt

P13195 - HEM1_RAT

Protein

5-aminolevulinate synthase, nonspecific, mitochondrial

Gene

Alas1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (27 Sep 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei219 – 2191SubstrateBy similarity
    Binding sitei336 – 3361SubstrateBy similarity
    Binding sitei355 – 3551SubstrateBy similarity
    Binding sitei388 – 3881Pyridoxal phosphateBy similarity
    Binding sitei416 – 4161Pyridoxal phosphateBy similarity
    Binding sitei444 – 4441Pyridoxal phosphateBy similarity
    Active sitei447 – 4471By similarity
    Binding sitei476 – 4761Pyridoxal phosphateBy similarity
    Binding sitei477 – 4771Pyridoxal phosphateBy similarity
    Binding sitei564 – 5641SubstrateBy similarity

    GO - Molecular functioni

    1. 5-aminolevulinate synthase activity Source: RGD
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. cellular response to insulin stimulus Source: RGD
    2. cellular response to organic cyclic compound Source: RGD
    3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
    4. response to cAMP Source: RGD
    5. response to cobalt ion Source: RGD
    6. response to drug Source: RGD
    7. response to ethanol Source: RGD
    8. response to gonadotropin Source: RGD
    9. response to herbicide Source: RGD
    10. response to hypoxia Source: RGD
    11. response to nickel cation Source: RGD
    12. response to nutrient levels Source: RGD
    13. response to organic cyclic compound Source: RGD
    14. response to organic substance Source: RGD
    15. response to platinum ion Source: RGD

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Heme biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00375.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-aminolevulinate synthase, nonspecific, mitochondrial (EC:2.3.1.37)
    Short name:
    ALAS-H
    Alternative name(s):
    5-aminolevulinic acid synthase 1
    Delta-ALA synthase 1
    Delta-aminolevulinate synthase 1
    Gene namesi
    Name:Alas1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi68392. Alas1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5656MitochondrionAdd
    BLAST
    Chaini57 – 6425865-aminolevulinate synthase, nonspecific, mitochondrialPRO_0000001232Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei447 – 4471N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PRIDEiP13195.

    Expressioni

    Gene expression databases

    GenevestigatoriP13195.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP13195.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG005954.
    KOiK00643.
    PhylomeDBiP13195.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR015118. 5aminolev_synth_preseq.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    PF09029. Preseq_ALAS. 2 hits.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13195-1 [UniParc]FASTAAdd to Basket

    « Hide

    METVVRRCPF LSRVPQAFLQ KAGKSLLFYA QNCPKMMEVG AKPAPRTVST    50
    SAAQCQQVKE TPPANEKEKT AKAAVQQAPD ESQMAQTPDG TQLPPGHPSP 100
    STSQSSGSKC PFLAAQLSQT GSSVFRKASL ELQEDVQEMH AVRKEVAQSP 150
    VLPSLVNAKR DGEGPSPLLK NFQDIMRKQR PERVSHLLQD NLPKSVSTFQ 200
    YDHFFEKKID EKKNDHTYRV FKTVNRRAQI FPMADDYTDS LITKKQVSVW 250
    CSNDYLGMSR HPRVCGAVIE TVKQHGAGAG GTRNISGTSK FHVELEQELA 300
    DLHGKDAALL FSSCFVANDS TLFTLAKMMP GCEIYSDSGN HASMIQGIRN 350
    SRVPKYIFRH NDVNHLRELL QRSDPSVPKI VAFETVHSMD GAVCPLEELC 400
    DVAHEFGAIT FVDEVHAVGL YGASGGGIGD RDGVMPKMDI ISGTLGKAFG 450
    CVGGYIASTS LLIDTVRSYA AGFIFTTSLP PMLLAGALES VRILKSNEGR 500
    ALRRQHQRNV KLMRQMLMDA GLPVIHCPSH IIPVRVADAA KNTEICDELM 550
    TRHNIYVQAI NYPTVPRGEE LLRIAPTPHH TPQMMNFFLE KLLLTWKRVG 600
    LELKPHSSAE CNFCRRPLHF EVMSEREKAY FSGMSKMVSA QA 642
    Length:642
    Mass (Da):71,021
    Last modified:September 27, 2004 - v2
    Checksum:iB28FC05D5F834886
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti118 – 13619SQTGS…LQEDV → ARRAAASSARPVWSFRRTW in AAA40790. (PubMed:3356687)CuratedAdd
    BLAST
    Sequence conflicti144 – 1441K → T in AAA40790. (PubMed:3356687)Curated
    Sequence conflicti195 – 1951S → V in AAA40790. (PubMed:3356687)Curated
    Sequence conflicti244 – 2452KK → NN in AAA40790. (PubMed:3356687)Curated
    Sequence conflicti251 – 2511C → S in AAA40790. (PubMed:3356687)Curated
    Sequence conflicti377 – 3771V → L in AAA40724. (PubMed:3182776)Curated
    Sequence conflicti587 – 5871F → Y in AAA40790. (PubMed:3356687)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03190 mRNA. Translation: AAA40790.1.
    J04044 mRNA. Translation: AAA40724.1.
    BC061793 mRNA. Translation: AAH61793.1.
    PIRiA28191. SYRTAL.
    RefSeqiNP_077810.2. NM_024484.2.
    UniGeneiRn.97126.

    Genome annotation databases

    GeneIDi65155.
    KEGGirno:65155.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03190 mRNA. Translation: AAA40790.1 .
    J04044 mRNA. Translation: AAA40724.1 .
    BC061793 mRNA. Translation: AAH61793.1 .
    PIRi A28191. SYRTAL.
    RefSeqi NP_077810.2. NM_024484.2.
    UniGenei Rn.97126.

    3D structure databases

    ProteinModelPortali P13195.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P13195.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 65155.
    KEGGi rno:65155.

    Organism-specific databases

    CTDi 211.
    RGDi 68392. Alas1.

    Phylogenomic databases

    HOVERGENi HBG005954.
    KOi K00643.
    PhylomeDBi P13195.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00375 .

    Miscellaneous databases

    NextBioi 614001.
    PROi P13195.

    Gene expression databases

    Genevestigatori P13195.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR015118. 5aminolev_synth_preseq.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    PF09029. Preseq_ALAS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of 5-aminolevulinate synthase mRNA in different rat tissues."
      Srivastava G., Borthwick I.A., Maguire D.J., Elferink C.J., Bawden M.J., Mercer J.F.B., May B.K.
      J. Biol. Chem. 263:5202-5209(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Structure, turnover, and heme-mediated suppression of the level of mRNA encoding rat liver delta-aminolevulinate synthase."
      Yamamoto M., Kure S., Engel J.D., Hiraga K.
      J. Biol. Chem. 263:15973-15979(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiHEM1_RAT
    AccessioniPrimary (citable) accession number: P13195
    Secondary accession number(s): Q6P782
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: September 27, 2004
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3