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P13188

- SYQ_YEAST

UniProt

P13188 - SYQ_YEAST

Protein

Glutamine--tRNA ligase

Gene

GLN4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei498 – 4981ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamine-tRNA ligase activity Source: SGD

    GO - Biological processi

    1. glutaminyl-tRNA aminoacylation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33684-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--tRNA ligase (EC:6.1.1.18)
    Alternative name(s):
    Glutaminyl-tRNA synthetase
    Short name:
    GlnRS
    Gene namesi
    Name:GLN4
    Ordered Locus Names:YOR168W
    ORF Names:O3601
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR168w.
    SGDiS000005694. GLN4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. mitochondrion Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 809809Glutamine--tRNA ligasePRO_0000195866Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei378 – 3781Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP13188.
    PaxDbiP13188.
    PeptideAtlasiP13188.

    Expressioni

    Gene expression databases

    GenevestigatoriP13188.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-18789,EBI-18789

    Protein-protein interaction databases

    BioGridi34564. 18 interactions.
    IntActiP13188. 1 interaction.
    MINTiMINT-663565.
    STRINGi4932.YOR168W.

    Structurei

    Secondary structure

    1
    809
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1310
    Helixi18 – 247
    Helixi28 – 3912
    Helixi49 – 6214
    Helixi70 – 789
    Helixi85 – 9814
    Helixi99 – 1013
    Helixi104 – 1096
    Turni110 – 1145
    Helixi119 – 13214
    Helixi134 – 1407
    Helixi141 – 1444
    Helixi145 – 1539
    Helixi156 – 1583
    Helixi166 – 17813
    Helixi183 – 1853
    Helixi222 – 2254
    Helixi238 – 2403
    Helixi241 – 2488
    Beta strandi253 – 2564
    Beta strandi260 – 2623
    Helixi267 – 28115
    Beta strandi285 – 2906
    Helixi300 – 31213
    Beta strandi318 – 3225
    Helixi323 – 3264
    Helixi327 – 33913
    Beta strandi343 – 3464
    Helixi350 – 3567
    Helixi373 – 3764
    Helixi379 – 39012
    Beta strandi400 – 4034
    Helixi412 – 4143
    Beta strandi418 – 4225
    Turni428 – 4303
    Beta strandi435 – 4384
    Helixi440 – 45011
    Beta strandi456 – 4594
    Helixi460 – 4656
    Helixi466 – 47510
    Beta strandi483 – 4864
    Beta strandi489 – 4946
    Helixi498 – 5069
    Beta strandi509 – 5124
    Helixi521 – 5277
    Helixi531 – 54111
    Beta strandi548 – 5514
    Helixi552 – 56615
    Beta strandi572 – 58110
    Beta strandi590 – 5978
    Helixi601 – 6033
    Beta strandi605 – 6106
    Beta strandi612 – 6176
    Helixi618 – 6203
    Beta strandi632 – 6343
    Beta strandi639 – 6413
    Beta strandi648 – 6558
    Beta strandi661 – 6688
    Helixi692 – 6943
    Beta strandi698 – 7069
    Beta strandi709 – 7135
    Helixi715 – 7173
    Helixi722 – 7254
    Beta strandi730 – 73910
    Helixi743 – 7486
    Helixi755 – 7584
    Helixi771 – 7733
    Beta strandi776 – 7783
    Turni779 – 7813
    Beta strandi782 – 7865
    Beta strandi792 – 7943
    Beta strandi796 – 8016

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TL4X-ray2.30X1-187[»]
    4H3SX-ray2.15A1-809[»]
    ProteinModelPortaliP13188.
    SMRiP13188. Positions 2-187, 215-809.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi258 – 26811"HIGH" regionAdd
    BLAST
    Motifi495 – 4995"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0008.
    GeneTreeiENSGT00550000074972.
    HOGENOMiHOG000259233.
    KOiK01886.
    OMAiRMQKRAK.
    OrthoDBiEOG7MD4ZK.

    Family and domain databases

    Gene3Di1.10.1160.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR004514. Gln-tRNA-synth.
    IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
    IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    PF04558. tRNA_synt_1c_R1. 1 hit.
    PF04557. tRNA_synt_1c_R2. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF50715. SSF50715. 1 hit.
    TIGRFAMsiTIGR00440. glnS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13188-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSVEELTQL FSQVGFEDKK VKEIVKNKKV SDSLYKLIKE TPSDYQWNKS    50
    TRALVHNLAS FVKGTDLPKS ELIVNGIING DLKTSLQVDA AFKYVKANGE 100
    ASTKMGMNEN SGVGIEITED QVRNYVMQYI QENKERILTE RYKLVPGIFA 150
    DVKNLKELKW ADPRSFKPII DQEVLKLLGP KDERDLIKKK TKNNEKKKTN 200
    SAKKSSDNSA SSGPKRTMFN EGFLGDLHKV GENPQAYPEL MKEHLEVTGG 250
    KVRTRFPPEP NGYLHIGHSK AIMVNFGYAK YHNGTCYLRF DDTNPEKEAP 300
    EYFESIKRMV SWLGFKPWKI TYSSDYFDEL YRLAEVLIKN GKAYVCHCTA 350
    EEIKRGRGIK EDGTPGGERY ACKHRDQSIE QNLQEFRDMR DGKYKPGEAI 400
    LRMKQDLNSP SPQMWDLIAY RVLNAPHPRT GTKWRIYPTY DFTHCLVDSM 450
    ENITHSLCTT EFYLSRESYE WLCDQVHVFR PAQREYGRLN ITGTVLSKRK 500
    IAQLVDEKFV RGWDDPRLFT LEAIRRRGVP PGAILSFINT LGVTTSTTNI 550
    QVVRFESAVR KYLEDTTPRL MFVLDPVEVV VDNLSDDYEE LATIPYRPGT 600
    PEFGERTVPF TNKFYIERSD FSENVDDKEF FRLTPNQPVG LIKVSHTVSF 650
    KSLEKDEAGK IIRIHVNYDN KVEEGSKPKK PKTYIQWVPI SSKYNSPLRV 700
    TETRVYNQLF KSENPSSHPE GFLKDINPES EVVYKESVME HNFGDVVKNS 750
    PWVVDSVKNS EFYVEEDKDS KEVCRFQAMR VGYFTLDKES TTSKVILNRI 800
    VSLKDATSK 809
    Length:809
    Mass (Da):93,133
    Last modified:November 1, 1997 - v2
    Checksum:iC7AB13D02BC483F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti179 – 1791G → Q(PubMed:3301841)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29184 Genomic DNA. Translation: AAA34646.1.
    M29185 Genomic DNA. No translation available.
    U55021 Genomic DNA. Translation: AAB47415.1.
    Z75076 Genomic DNA. Translation: CAA99374.1.
    BK006948 Genomic DNA. Translation: DAA10942.1.
    PIRiS67056. SYBYQT.
    RefSeqiNP_014811.3. NM_001183587.3.

    Genome annotation databases

    EnsemblFungiiYOR168W; YOR168W; YOR168W.
    GeneIDi854339.
    KEGGisce:YOR168W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29184 Genomic DNA. Translation: AAA34646.1 .
    M29185 Genomic DNA. No translation available.
    U55021 Genomic DNA. Translation: AAB47415.1 .
    Z75076 Genomic DNA. Translation: CAA99374.1 .
    BK006948 Genomic DNA. Translation: DAA10942.1 .
    PIRi S67056. SYBYQT.
    RefSeqi NP_014811.3. NM_001183587.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TL4 X-ray 2.30 X 1-187 [» ]
    4H3S X-ray 2.15 A 1-809 [» ]
    ProteinModelPortali P13188.
    SMRi P13188. Positions 2-187, 215-809.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34564. 18 interactions.
    IntActi P13188. 1 interaction.
    MINTi MINT-663565.
    STRINGi 4932.YOR168W.

    Proteomic databases

    MaxQBi P13188.
    PaxDbi P13188.
    PeptideAtlasi P13188.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR168W ; YOR168W ; YOR168W .
    GeneIDi 854339.
    KEGGi sce:YOR168W.

    Organism-specific databases

    CYGDi YOR168w.
    SGDi S000005694. GLN4.

    Phylogenomic databases

    eggNOGi COG0008.
    GeneTreei ENSGT00550000074972.
    HOGENOMi HOG000259233.
    KOi K01886.
    OMAi RMQKRAK.
    OrthoDBi EOG7MD4ZK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33684-MONOMER.

    Miscellaneous databases

    NextBioi 976411.
    PROi P13188.

    Gene expression databases

    Genevestigatori P13188.

    Family and domain databases

    Gene3Di 1.10.1160.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR004514. Gln-tRNA-synth.
    IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
    IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    PF04558. tRNA_synt_1c_R1. 1 hit.
    PF04557. tRNA_synt_1c_R2. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF50715. SSF50715. 1 hit.
    TIGRFAMsi TIGR00440. glnS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene for yeast glutamine tRNA synthetase encodes a large amino-terminal extension and provides a strong confirmation of the signature sequence for a group of the aminoacyl-tRNA synthetases."
      Ludmerer S.W., Schimmel P.
      J. Biol. Chem. 262:10801-10806(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV."
      Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
      Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1678.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-378, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYQ_YEAST
    AccessioniPrimary (citable) accession number: P13188
    Secondary accession number(s): D6W2M6, Q12005
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 141 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 37500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3