SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P13188

- SYQ_YEAST

UniProt

P13188 - SYQ_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutamine--tRNA ligase

Gene
GLN4, YOR168W, O3601
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei498 – 4981ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamine-tRNA ligase activity Source: SGD

GO - Biological processi

  1. glutaminyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33684-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligase (EC:6.1.1.18)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
Gene namesi
Name:GLN4
Ordered Locus Names:YOR168W
ORF Names:O3601
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR168w.
SGDiS000005694. GLN4.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 809809Glutamine--tRNA ligasePRO_0000195866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei378 – 3781Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13188.
PaxDbiP13188.
PeptideAtlasiP13188.

Expressioni

Gene expression databases

GenevestigatoriP13188.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-18789,EBI-18789

Protein-protein interaction databases

BioGridi34564. 18 interactions.
IntActiP13188. 1 interaction.
MINTiMINT-663565.
STRINGi4932.YOR168W.

Structurei

Secondary structure

1
809
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1310
Helixi18 – 247
Helixi28 – 3912
Helixi49 – 6214
Helixi70 – 789
Helixi85 – 9814
Helixi99 – 1013
Helixi104 – 1096
Turni110 – 1145
Helixi119 – 13214
Helixi134 – 1407
Helixi141 – 1444
Helixi145 – 1539
Helixi156 – 1583
Helixi166 – 17813
Helixi183 – 1853
Helixi222 – 2254
Helixi238 – 2403
Helixi241 – 2488
Beta strandi253 – 2564
Beta strandi260 – 2623
Helixi267 – 28115
Beta strandi285 – 2906
Helixi300 – 31213
Beta strandi318 – 3225
Helixi323 – 3264
Helixi327 – 33913
Beta strandi343 – 3464
Helixi350 – 3567
Helixi373 – 3764
Helixi379 – 39012
Beta strandi400 – 4034
Helixi412 – 4143
Beta strandi418 – 4225
Turni428 – 4303
Beta strandi435 – 4384
Helixi440 – 45011
Beta strandi456 – 4594
Helixi460 – 4656
Helixi466 – 47510
Beta strandi483 – 4864
Beta strandi489 – 4946
Helixi498 – 5069
Beta strandi509 – 5124
Helixi521 – 5277
Helixi531 – 54111
Beta strandi548 – 5514
Helixi552 – 56615
Beta strandi572 – 58110
Beta strandi590 – 5978
Helixi601 – 6033
Beta strandi605 – 6106
Beta strandi612 – 6176
Helixi618 – 6203
Beta strandi632 – 6343
Beta strandi639 – 6413
Beta strandi648 – 6558
Beta strandi661 – 6688
Helixi692 – 6943
Beta strandi698 – 7069
Beta strandi709 – 7135
Helixi715 – 7173
Helixi722 – 7254
Beta strandi730 – 73910
Helixi743 – 7486
Helixi755 – 7584
Helixi771 – 7733
Beta strandi776 – 7783
Turni779 – 7813
Beta strandi782 – 7865
Beta strandi792 – 7943
Beta strandi796 – 8016

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TL4X-ray2.30X1-187[»]
4H3SX-ray2.15A1-809[»]
ProteinModelPortaliP13188.
SMRiP13188. Positions 2-187, 215-809.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi258 – 26811"HIGH" regionAdd
BLAST
Motifi495 – 4995"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
GeneTreeiENSGT00550000074972.
HOGENOMiHOG000259233.
KOiK01886.
OMAiRMQKRAK.
OrthoDBiEOG7MD4ZK.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13188-1 [UniParc]FASTAAdd to Basket

« Hide

MSSVEELTQL FSQVGFEDKK VKEIVKNKKV SDSLYKLIKE TPSDYQWNKS    50
TRALVHNLAS FVKGTDLPKS ELIVNGIING DLKTSLQVDA AFKYVKANGE 100
ASTKMGMNEN SGVGIEITED QVRNYVMQYI QENKERILTE RYKLVPGIFA 150
DVKNLKELKW ADPRSFKPII DQEVLKLLGP KDERDLIKKK TKNNEKKKTN 200
SAKKSSDNSA SSGPKRTMFN EGFLGDLHKV GENPQAYPEL MKEHLEVTGG 250
KVRTRFPPEP NGYLHIGHSK AIMVNFGYAK YHNGTCYLRF DDTNPEKEAP 300
EYFESIKRMV SWLGFKPWKI TYSSDYFDEL YRLAEVLIKN GKAYVCHCTA 350
EEIKRGRGIK EDGTPGGERY ACKHRDQSIE QNLQEFRDMR DGKYKPGEAI 400
LRMKQDLNSP SPQMWDLIAY RVLNAPHPRT GTKWRIYPTY DFTHCLVDSM 450
ENITHSLCTT EFYLSRESYE WLCDQVHVFR PAQREYGRLN ITGTVLSKRK 500
IAQLVDEKFV RGWDDPRLFT LEAIRRRGVP PGAILSFINT LGVTTSTTNI 550
QVVRFESAVR KYLEDTTPRL MFVLDPVEVV VDNLSDDYEE LATIPYRPGT 600
PEFGERTVPF TNKFYIERSD FSENVDDKEF FRLTPNQPVG LIKVSHTVSF 650
KSLEKDEAGK IIRIHVNYDN KVEEGSKPKK PKTYIQWVPI SSKYNSPLRV 700
TETRVYNQLF KSENPSSHPE GFLKDINPES EVVYKESVME HNFGDVVKNS 750
PWVVDSVKNS EFYVEEDKDS KEVCRFQAMR VGYFTLDKES TTSKVILNRI 800
VSLKDATSK 809
Length:809
Mass (Da):93,133
Last modified:November 1, 1997 - v2
Checksum:iC7AB13D02BC483F6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791G → Q1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29184 Genomic DNA. Translation: AAA34646.1.
M29185 Genomic DNA. No translation available.
U55021 Genomic DNA. Translation: AAB47415.1.
Z75076 Genomic DNA. Translation: CAA99374.1.
BK006948 Genomic DNA. Translation: DAA10942.1.
PIRiS67056. SYBYQT.
RefSeqiNP_014811.3. NM_001183587.3.

Genome annotation databases

EnsemblFungiiYOR168W; YOR168W; YOR168W.
GeneIDi854339.
KEGGisce:YOR168W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29184 Genomic DNA. Translation: AAA34646.1 .
M29185 Genomic DNA. No translation available.
U55021 Genomic DNA. Translation: AAB47415.1 .
Z75076 Genomic DNA. Translation: CAA99374.1 .
BK006948 Genomic DNA. Translation: DAA10942.1 .
PIRi S67056. SYBYQT.
RefSeqi NP_014811.3. NM_001183587.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TL4 X-ray 2.30 X 1-187 [» ]
4H3S X-ray 2.15 A 1-809 [» ]
ProteinModelPortali P13188.
SMRi P13188. Positions 2-187, 215-809.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34564. 18 interactions.
IntActi P13188. 1 interaction.
MINTi MINT-663565.
STRINGi 4932.YOR168W.

Proteomic databases

MaxQBi P13188.
PaxDbi P13188.
PeptideAtlasi P13188.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR168W ; YOR168W ; YOR168W .
GeneIDi 854339.
KEGGi sce:YOR168W.

Organism-specific databases

CYGDi YOR168w.
SGDi S000005694. GLN4.

Phylogenomic databases

eggNOGi COG0008.
GeneTreei ENSGT00550000074972.
HOGENOMi HOG000259233.
KOi K01886.
OMAi RMQKRAK.
OrthoDBi EOG7MD4ZK.

Enzyme and pathway databases

BioCyci YEAST:G3O-33684-MONOMER.

Miscellaneous databases

NextBioi 976411.
PROi P13188.

Gene expression databases

Genevestigatori P13188.

Family and domain databases

Gene3Di 1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF50715. SSF50715. 1 hit.
TIGRFAMsi TIGR00440. glnS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene for yeast glutamine tRNA synthetase encodes a large amino-terminal extension and provides a strong confirmation of the signature sequence for a group of the aminoacyl-tRNA synthetases."
    Ludmerer S.W., Schimmel P.
    J. Biol. Chem. 262:10801-10806(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV."
    Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
    Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1678.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-378, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYQ_YEAST
AccessioniPrimary (citable) accession number: P13188
Secondary accession number(s): D6W2M6, Q12005
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 37500 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi