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P13188 (SYQ_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:GLN4
Ordered Locus Names:YOR168W
ORF Names:O3601
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

Miscellaneous

Present with 37500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-18789,EBI-18789

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 809809Glutamine--tRNA ligase
PRO_0000195866

Regions

Motif258 – 26811"HIGH" region
Motif495 – 4995"KMSKS" region

Sites

Binding site4981ATP By similarity

Amino acid modifications

Modified residue3781Phosphoserine Ref.6

Experimental info

Sequence conflict1791G → Q Ref.1

Secondary structure

.................................................................................................................................... 809
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13188 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: C7AB13D02BC483F6

FASTA80993,133
        10         20         30         40         50         60 
MSSVEELTQL FSQVGFEDKK VKEIVKNKKV SDSLYKLIKE TPSDYQWNKS TRALVHNLAS 

        70         80         90        100        110        120 
FVKGTDLPKS ELIVNGIING DLKTSLQVDA AFKYVKANGE ASTKMGMNEN SGVGIEITED 

       130        140        150        160        170        180 
QVRNYVMQYI QENKERILTE RYKLVPGIFA DVKNLKELKW ADPRSFKPII DQEVLKLLGP 

       190        200        210        220        230        240 
KDERDLIKKK TKNNEKKKTN SAKKSSDNSA SSGPKRTMFN EGFLGDLHKV GENPQAYPEL 

       250        260        270        280        290        300 
MKEHLEVTGG KVRTRFPPEP NGYLHIGHSK AIMVNFGYAK YHNGTCYLRF DDTNPEKEAP 

       310        320        330        340        350        360 
EYFESIKRMV SWLGFKPWKI TYSSDYFDEL YRLAEVLIKN GKAYVCHCTA EEIKRGRGIK 

       370        380        390        400        410        420 
EDGTPGGERY ACKHRDQSIE QNLQEFRDMR DGKYKPGEAI LRMKQDLNSP SPQMWDLIAY 

       430        440        450        460        470        480 
RVLNAPHPRT GTKWRIYPTY DFTHCLVDSM ENITHSLCTT EFYLSRESYE WLCDQVHVFR 

       490        500        510        520        530        540 
PAQREYGRLN ITGTVLSKRK IAQLVDEKFV RGWDDPRLFT LEAIRRRGVP PGAILSFINT 

       550        560        570        580        590        600 
LGVTTSTTNI QVVRFESAVR KYLEDTTPRL MFVLDPVEVV VDNLSDDYEE LATIPYRPGT 

       610        620        630        640        650        660 
PEFGERTVPF TNKFYIERSD FSENVDDKEF FRLTPNQPVG LIKVSHTVSF KSLEKDEAGK 

       670        680        690        700        710        720 
IIRIHVNYDN KVEEGSKPKK PKTYIQWVPI SSKYNSPLRV TETRVYNQLF KSENPSSHPE 

       730        740        750        760        770        780 
GFLKDINPES EVVYKESVME HNFGDVVKNS PWVVDSVKNS EFYVEEDKDS KEVCRFQAMR 

       790        800 
VGYFTLDKES TTSKVILNRI VSLKDATSK 

« Hide

References

« Hide 'large scale' references
[1]"Gene for yeast glutamine tRNA synthetase encodes a large amino-terminal extension and provides a strong confirmation of the signature sequence for a group of the aminoacyl-tRNA synthetases."
Ludmerer S.W., Schimmel P.
J. Biol. Chem. 262:10801-10806(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV."
Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1678.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-378, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M29184 Genomic DNA. Translation: AAA34646.1.
M29185 Genomic DNA. No translation available.
U55021 Genomic DNA. Translation: AAB47415.1.
Z75076 Genomic DNA. Translation: CAA99374.1.
BK006948 Genomic DNA. Translation: DAA10942.1.
PIRSYBYQT. S67056.
RefSeqNP_014811.3. NM_001183587.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3TL4X-ray2.30X1-187[»]
4H3SX-ray2.15A1-809[»]
ProteinModelPortalP13188.
SMRP13188. Positions 2-187, 215-809.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34564. 18 interactions.
IntActP13188. 1 interaction.
MINTMINT-663565.
STRING4932.YOR168W.

Proteomic databases

PaxDbP13188.
PeptideAtlasP13188.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR168W; YOR168W; YOR168W.
GeneID854339.
KEGGsce:YOR168W.

Organism-specific databases

CYGDYOR168w.
SGDS000005694. GLN4.

Phylogenomic databases

eggNOGCOG0008.
GeneTreeENSGT00550000074972.
HOGENOMHOG000259233.
KOK01886.
OMAYEWLCDA.
OrthoDBEOG7MD4ZK.

Enzyme and pathway databases

BioCycYEAST:G3O-33684-MONOMER.

Gene expression databases

GenevestigatorP13188.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976411.
PROP13188.

Entry information

Entry nameSYQ_YEAST
AccessionPrimary (citable) accession number: P13188
Secondary accession number(s): D6W2M6, Q12005
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1997
Last modified: March 19, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries