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Protein

Glutamine--tRNA ligase

Gene

GLN4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei498ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamine-tRNA ligase activity Source: SGD

GO - Biological processi

  • glutaminyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33684-MONOMER.
BRENDAi6.1.1.18. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligase (EC:6.1.1.18)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
Gene namesi
Name:GLN4
Ordered Locus Names:YOR168W
ORF Names:O3601
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR168W.
SGDiS000005694. GLN4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001958661 – 809Glutamine--tRNA ligaseAdd BLAST809

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei378PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13188.
PRIDEiP13188.

PTM databases

iPTMnetiP13188.

Interactioni

Protein-protein interaction databases

BioGridi34564. 18 interactors.
IntActiP13188. 1 interactor.
MINTiMINT-663565.

Structurei

Secondary structure

1809
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 13Combined sources10
Helixi18 – 24Combined sources7
Helixi28 – 39Combined sources12
Helixi49 – 62Combined sources14
Helixi70 – 78Combined sources9
Helixi85 – 98Combined sources14
Helixi99 – 101Combined sources3
Helixi104 – 109Combined sources6
Turni110 – 114Combined sources5
Helixi119 – 132Combined sources14
Helixi134 – 140Combined sources7
Helixi141 – 144Combined sources4
Helixi145 – 153Combined sources9
Helixi156 – 158Combined sources3
Helixi166 – 178Combined sources13
Helixi183 – 185Combined sources3
Helixi222 – 225Combined sources4
Helixi238 – 240Combined sources3
Helixi241 – 248Combined sources8
Beta strandi253 – 256Combined sources4
Beta strandi260 – 262Combined sources3
Helixi267 – 281Combined sources15
Beta strandi285 – 290Combined sources6
Helixi300 – 312Combined sources13
Beta strandi318 – 322Combined sources5
Helixi323 – 326Combined sources4
Helixi327 – 339Combined sources13
Beta strandi343 – 346Combined sources4
Helixi350 – 356Combined sources7
Helixi373 – 376Combined sources4
Helixi379 – 390Combined sources12
Beta strandi400 – 403Combined sources4
Helixi412 – 414Combined sources3
Beta strandi418 – 422Combined sources5
Turni428 – 430Combined sources3
Beta strandi435 – 438Combined sources4
Helixi440 – 450Combined sources11
Beta strandi456 – 459Combined sources4
Helixi460 – 465Combined sources6
Helixi466 – 475Combined sources10
Beta strandi483 – 486Combined sources4
Beta strandi489 – 494Combined sources6
Helixi498 – 506Combined sources9
Beta strandi509 – 512Combined sources4
Helixi521 – 527Combined sources7
Helixi531 – 541Combined sources11
Beta strandi548 – 551Combined sources4
Helixi552 – 566Combined sources15
Beta strandi572 – 581Combined sources10
Beta strandi590 – 597Combined sources8
Helixi601 – 603Combined sources3
Beta strandi605 – 610Combined sources6
Beta strandi612 – 617Combined sources6
Helixi618 – 620Combined sources3
Beta strandi632 – 634Combined sources3
Beta strandi639 – 641Combined sources3
Beta strandi648 – 655Combined sources8
Beta strandi661 – 668Combined sources8
Helixi692 – 694Combined sources3
Beta strandi698 – 706Combined sources9
Beta strandi709 – 713Combined sources5
Helixi715 – 717Combined sources3
Helixi722 – 725Combined sources4
Beta strandi730 – 739Combined sources10
Helixi743 – 748Combined sources6
Helixi755 – 758Combined sources4
Helixi771 – 773Combined sources3
Beta strandi776 – 778Combined sources3
Turni779 – 781Combined sources3
Beta strandi782 – 786Combined sources5
Beta strandi792 – 794Combined sources3
Beta strandi796 – 801Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TL4X-ray2.30X1-187[»]
4H3SX-ray2.15A1-809[»]
ProteinModelPortaliP13188.
SMRiP13188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi258 – 268"HIGH" regionAdd BLAST11
Motifi495 – 499"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074972.
HOGENOMiHOG000259233.
InParanoidiP13188.
KOiK01886.
OMAiHCTYDPE.
OrthoDBiEOG092C0PFH.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13188-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVEELTQL FSQVGFEDKK VKEIVKNKKV SDSLYKLIKE TPSDYQWNKS
60 70 80 90 100
TRALVHNLAS FVKGTDLPKS ELIVNGIING DLKTSLQVDA AFKYVKANGE
110 120 130 140 150
ASTKMGMNEN SGVGIEITED QVRNYVMQYI QENKERILTE RYKLVPGIFA
160 170 180 190 200
DVKNLKELKW ADPRSFKPII DQEVLKLLGP KDERDLIKKK TKNNEKKKTN
210 220 230 240 250
SAKKSSDNSA SSGPKRTMFN EGFLGDLHKV GENPQAYPEL MKEHLEVTGG
260 270 280 290 300
KVRTRFPPEP NGYLHIGHSK AIMVNFGYAK YHNGTCYLRF DDTNPEKEAP
310 320 330 340 350
EYFESIKRMV SWLGFKPWKI TYSSDYFDEL YRLAEVLIKN GKAYVCHCTA
360 370 380 390 400
EEIKRGRGIK EDGTPGGERY ACKHRDQSIE QNLQEFRDMR DGKYKPGEAI
410 420 430 440 450
LRMKQDLNSP SPQMWDLIAY RVLNAPHPRT GTKWRIYPTY DFTHCLVDSM
460 470 480 490 500
ENITHSLCTT EFYLSRESYE WLCDQVHVFR PAQREYGRLN ITGTVLSKRK
510 520 530 540 550
IAQLVDEKFV RGWDDPRLFT LEAIRRRGVP PGAILSFINT LGVTTSTTNI
560 570 580 590 600
QVVRFESAVR KYLEDTTPRL MFVLDPVEVV VDNLSDDYEE LATIPYRPGT
610 620 630 640 650
PEFGERTVPF TNKFYIERSD FSENVDDKEF FRLTPNQPVG LIKVSHTVSF
660 670 680 690 700
KSLEKDEAGK IIRIHVNYDN KVEEGSKPKK PKTYIQWVPI SSKYNSPLRV
710 720 730 740 750
TETRVYNQLF KSENPSSHPE GFLKDINPES EVVYKESVME HNFGDVVKNS
760 770 780 790 800
PWVVDSVKNS EFYVEEDKDS KEVCRFQAMR VGYFTLDKES TTSKVILNRI

VSLKDATSK
Length:809
Mass (Da):93,133
Last modified:November 1, 1997 - v2
Checksum:iC7AB13D02BC483F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179G → Q (PubMed:3301841).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29184 Genomic DNA. Translation: AAA34646.1.
M29185 Genomic DNA. No translation available.
U55021 Genomic DNA. Translation: AAB47415.1.
Z75076 Genomic DNA. Translation: CAA99374.1.
BK006948 Genomic DNA. Translation: DAA10942.1.
PIRiS67056. SYBYQT.
RefSeqiNP_014811.3. NM_001183587.3.

Genome annotation databases

EnsemblFungiiYOR168W; YOR168W; YOR168W.
GeneIDi854339.
KEGGisce:YOR168W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29184 Genomic DNA. Translation: AAA34646.1.
M29185 Genomic DNA. No translation available.
U55021 Genomic DNA. Translation: AAB47415.1.
Z75076 Genomic DNA. Translation: CAA99374.1.
BK006948 Genomic DNA. Translation: DAA10942.1.
PIRiS67056. SYBYQT.
RefSeqiNP_014811.3. NM_001183587.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TL4X-ray2.30X1-187[»]
4H3SX-ray2.15A1-809[»]
ProteinModelPortaliP13188.
SMRiP13188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34564. 18 interactors.
IntActiP13188. 1 interactor.
MINTiMINT-663565.

PTM databases

iPTMnetiP13188.

Proteomic databases

MaxQBiP13188.
PRIDEiP13188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR168W; YOR168W; YOR168W.
GeneIDi854339.
KEGGisce:YOR168W.

Organism-specific databases

EuPathDBiFungiDB:YOR168W.
SGDiS000005694. GLN4.

Phylogenomic databases

GeneTreeiENSGT00550000074972.
HOGENOMiHOG000259233.
InParanoidiP13188.
KOiK01886.
OMAiHCTYDPE.
OrthoDBiEOG092C0PFH.

Enzyme and pathway databases

BioCyciYEAST:G3O-33684-MONOMER.
BRENDAi6.1.1.18. 984.

Miscellaneous databases

PROiP13188.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYQ_YEAST
AccessioniPrimary (citable) accession number: P13188
Secondary accession number(s): D6W2M6, Q12005
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 37500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.