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P13186

- KIN2_YEAST

UniProt

P13186 - KIN2_YEAST

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Protein
Serine/threonine-protein kinase KIN2
Gene
KIN2, YLR096W, L8004.3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase involved in the regulation of exocytosis. Induces phosphorylation of SEC9 and its release from the plasma membrane to the cytosol.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281ATP By similarity
Active sitei248 – 2481Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi105 – 1139ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein serine/threonine kinase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. exocytosis Source: SGD
  2. protein autophosphorylation Source: SGD
  3. protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32246-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase KIN2 (EC:2.7.11.1)
Gene namesi
Name:KIN2
Ordered Locus Names:YLR096W
ORF Names:L8004.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR096w.
SGDiS000004086. KIN2.

Subcellular locationi

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11471147Serine/threonine-protein kinase KIN2
PRO_0000086133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Phosphoserine1 Publication
Modified residuei146 – 1461Phosphoserine1 Publication
Modified residuei549 – 5491Phosphoserine3 Publications
Modified residuei609 – 6091Phosphoserine1 Publication
Modified residuei888 – 8881Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13186.
PaxDbiP13186.
PeptideAtlasiP13186.

Expressioni

Gene expression databases

GenevestigatoriP13186.

Interactioni

Subunit structurei

Interacts with SEC9 and SRO7.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BCK2P333064EBI-9723,EBI-3480
BUD14P276373EBI-9723,EBI-20747
RIM15P435652EBI-9723,EBI-15150

Protein-protein interaction databases

BioGridi31369. 153 interactions.
DIPiDIP-6276N.
IntActiP13186. 52 interactions.
MINTiMINT-604572.
STRINGi4932.YLR096W.

Structurei

3D structure databases

ProteinModelPortaliP13186.
SMRiP13186. Positions 93-416.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 377279Protein kinase
Add
BLAST
Domaini1098 – 114750KA1
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi527 – 53610Poly-Gln

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00730000111001.
HOGENOMiHOG000207352.
KOiK08286.
OrthoDBiEOG793BK1.

Family and domain databases

Gene3Di3.30.310.80. 2 hits.
InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13186-1 [UniParc]FASTAAdd to Basket

« Hide

MPNPNTADYL VNPNFRTSKG GSLSPTPEAF NDTRVAAPAT LRMMGKQSGP     50
RNDQQQAPLM PPADIKQGKE QAAQRQNDAS RPNGAVELRQ FHRRSLGDWE 100
FLETVGAGSM GKVKLVKHRQ TKEICVIKIV NRASKAYLHK QHSLPSPKNE 150
SEILERQKRL EKEIARDKRT VREASLGQIL YHPHICRLFE MCTMSNHFYM 200
LFEYVSGGQL LDYIIQHGSL KEHHARKFAR GIASALQYLH ANNIVHRDLK 250
IENIMISSSG EIKIIDFGLS NIFDYRKQLH TFCGSLYFAA PELLKAQPYT 300
GPEVDIWSFG IVLYVLVCGK VPFDDENSSI LHEKIKKGKV DYPSHLSIEV 350
ISLLTRMIVV DPLRRATLKN VVEHPWMNRG YDFKAPSYVP NRVPLTPEMI 400
DSQVLKEMYR LEFIDDIEDT RRSLIRLVTE KEYIQLSQEY WDKLSNAKGL 450
SSSLNNNYLN STAQQTLIQN HITSNPSQSG YNEPDSNFED PTLAYHPLLS 500
IYHLVSEMVA RKLAKLQRRQ ALALQAQAQQ RQQQQQVALG TKVALNNNSP 550
DIMTKMRSPQ KEVVPNPGIF QVPAIGTSGT SNNTNTSNKP PLHVMVPPKL 600
TIPEQAHTSP TSRKSSDIHT ELNGVLKSTP VPVSGEYQQR SASPVVGEHQ 650
EKNTIGGIFR RISQSGQSQH PTRQQEPLPE REPPTYMSKS NEISIKVPKS 700
HSRTISDYIP SARRYPSYVP NSVDVKQKPA KNTTIAPPIR SVSQKQNSDL 750
PALPQNAELI VQKQRQKLLQ ENLDKLQIND NDNNNVNAVV DGINNDNSDH 800
YLSVPKGRKL HPSARAKSVG HARRESLKFT RPPIPAALPP SDMTNDNGFL 850
GEANKERYNP VSSNFSTVPE DSTTYSNDTN NRLTSVYSQE LTEKQILEEA 900
SKAPPGSMPS IDYPKSMFLK GFFSVQTTSS KPLPIVRHNI ISVLTRMNID 950
FKEVKGGFIC VQQRPSIETA AVPVITTTGV GLDSGKAMDL QNSLDSQLSS 1000
SYHSTASSAS RNSSIKRQGS YKRGQNNIPL TPLATNTHQR NSSIPMSPNY 1050
GNQSNGTSGE LSSMSLDYVQ QQDDILTTSR AQNINNVNGQ TEQTNTSGIK 1100
ERPPIKFEIH IVKVRIVGLA GVHFKKVSGN TWLYKELASY ILKELNL 1147
Length:1,147
Mass (Da):128,338
Last modified:December 15, 1998 - v3
Checksum:iAC2660BF3CA69600
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2172QH → HD in AAA34723. 1 Publication
Sequence conflicti675 – 70733QEPLP…RTISD → SGTYSSKENLQHICQNQMKF PSKYRKAIVVLYQT1 Publication
Add
BLAST
Sequence conflicti756 – 7583NAE → KRQ in AAA34723. 1 Publication
Sequence conflicti805 – 8051P → PLSVP1 Publication
Sequence conflicti1034 – 10374ATNT → TTNSI1 Publication
Sequence conflicti1041 – 10422NS → KT in AAA34723. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M69018 Genomic DNA. Translation: AAA34723.1.
Z73268 Genomic DNA. Translation: CAA97659.1.
Z73269 Genomic DNA. Translation: CAA97661.1.
U53876 Genomic DNA. Translation: AAB67540.1.
BK006945 Genomic DNA. Translation: DAA09412.1.
PIRiS64930.
RefSeqiNP_013197.1. NM_001181983.1.

Genome annotation databases

EnsemblFungiiYLR096W; YLR096W; YLR096W.
GeneIDi850785.
KEGGisce:YLR096W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M69018 Genomic DNA. Translation: AAA34723.1 .
Z73268 Genomic DNA. Translation: CAA97659.1 .
Z73269 Genomic DNA. Translation: CAA97661.1 .
U53876 Genomic DNA. Translation: AAB67540.1 .
BK006945 Genomic DNA. Translation: DAA09412.1 .
PIRi S64930.
RefSeqi NP_013197.1. NM_001181983.1.

3D structure databases

ProteinModelPortali P13186.
SMRi P13186. Positions 93-416.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31369. 153 interactions.
DIPi DIP-6276N.
IntActi P13186. 52 interactions.
MINTi MINT-604572.
STRINGi 4932.YLR096W.

Proteomic databases

MaxQBi P13186.
PaxDbi P13186.
PeptideAtlasi P13186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR096W ; YLR096W ; YLR096W .
GeneIDi 850785.
KEGGi sce:YLR096W.

Organism-specific databases

CYGDi YLR096w.
SGDi S000004086. KIN2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00730000111001.
HOGENOMi HOG000207352.
KOi K08286.
OrthoDBi EOG793BK1.

Enzyme and pathway databases

BioCyci YEAST:G3O-32246-MONOMER.
BRENDAi 2.7.11.1. 984.

Miscellaneous databases

NextBioi 966981.
PROi P13186.

Gene expression databases

Genevestigatori P13186.

Family and domain databases

Gene3Di 3.30.310.80. 2 hits.
InterProi IPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF103243. SSF103243. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEi PS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "KIN1 and KIN2 protein kinases localize to the cytoplasmic face of the yeast plasma membrane."
    Tibbetts M., Donovan M., Roe S., Stiltner A.M., Hammond C.I.
    Exp. Cell Res. 213:93-99(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Characterization of the KIN2 gene product in Saccharomyces cerevisiae and comparison between the kinase activities of p145KIN1 and p145KIN2."
    Donovan M., Romano P., Tibbetts M., Hammond C.I.
    Yeast 10:113-124(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery."
    Elbert M., Rossi G., Brennwald P.
    Mol. Biol. Cell 16:532-549(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SEC9 AND SRO7.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-549; SER-609 AND SER-888, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-549, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND SER-549, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKIN2_YEAST
AccessioniPrimary (citable) accession number: P13186
Secondary accession number(s): D6VY96, Q12384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 15, 1998
Last modified: June 11, 2014
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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