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P13186

- KIN2_YEAST

UniProt

P13186 - KIN2_YEAST

Protein

Serine/threonine-protein kinase KIN2

Gene

KIN2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 3 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Serine/threonine protein kinase involved in the regulation of exocytosis. Induces phosphorylation of SEC9 and its release from the plasma membrane to the cytosol.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei128 – 1281ATPPROSITE-ProRule annotation
    Active sitei248 – 2481Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi105 – 1139ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: SGD

    GO - Biological processi

    1. exocytosis Source: SGD
    2. protein autophosphorylation Source: SGD
    3. protein phosphorylation Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Exocytosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32246-MONOMER.
    BRENDAi2.7.11.1. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase KIN2 (EC:2.7.11.1)
    Gene namesi
    Name:KIN2
    Ordered Locus Names:YLR096W
    ORF Names:L8004.3
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR096w.
    SGDiS000004086. KIN2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11471147Serine/threonine-protein kinase KIN2PRO_0000086133Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei22 – 221Phosphoserine1 Publication
    Modified residuei146 – 1461Phosphoserine1 Publication
    Modified residuei549 – 5491Phosphoserine3 Publications
    Modified residuei609 – 6091Phosphoserine1 Publication
    Modified residuei888 – 8881Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP13186.
    PaxDbiP13186.
    PeptideAtlasiP13186.

    Expressioni

    Gene expression databases

    GenevestigatoriP13186.

    Interactioni

    Subunit structurei

    Interacts with SEC9 and SRO7.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BCK2P333064EBI-9723,EBI-3480
    BUD14P276373EBI-9723,EBI-20747
    RIM15P435652EBI-9723,EBI-15150

    Protein-protein interaction databases

    BioGridi31369. 153 interactions.
    DIPiDIP-6276N.
    IntActiP13186. 52 interactions.
    MINTiMINT-604572.
    STRINGi4932.YLR096W.

    Structurei

    3D structure databases

    ProteinModelPortaliP13186.
    SMRiP13186. Positions 93-416.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini99 – 377279Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1098 – 114750KA1PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi527 – 53610Poly-Gln

    Sequence similaritiesi

    Contains 1 KA1 (kinase-associated) domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000111001.
    HOGENOMiHOG000207352.
    KOiK08286.
    OrthoDBiEOG793BK1.

    Family and domain databases

    Gene3Di3.30.310.80. 2 hits.
    InterProiIPR028375. KA1/Ssp2_C.
    IPR001772. KA1_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF02149. KA1. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF103243. SSF103243. 2 hits.
    SSF56112. SSF56112. 2 hits.
    PROSITEiPS50032. KA1. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13186-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPNPNTADYL VNPNFRTSKG GSLSPTPEAF NDTRVAAPAT LRMMGKQSGP     50
    RNDQQQAPLM PPADIKQGKE QAAQRQNDAS RPNGAVELRQ FHRRSLGDWE 100
    FLETVGAGSM GKVKLVKHRQ TKEICVIKIV NRASKAYLHK QHSLPSPKNE 150
    SEILERQKRL EKEIARDKRT VREASLGQIL YHPHICRLFE MCTMSNHFYM 200
    LFEYVSGGQL LDYIIQHGSL KEHHARKFAR GIASALQYLH ANNIVHRDLK 250
    IENIMISSSG EIKIIDFGLS NIFDYRKQLH TFCGSLYFAA PELLKAQPYT 300
    GPEVDIWSFG IVLYVLVCGK VPFDDENSSI LHEKIKKGKV DYPSHLSIEV 350
    ISLLTRMIVV DPLRRATLKN VVEHPWMNRG YDFKAPSYVP NRVPLTPEMI 400
    DSQVLKEMYR LEFIDDIEDT RRSLIRLVTE KEYIQLSQEY WDKLSNAKGL 450
    SSSLNNNYLN STAQQTLIQN HITSNPSQSG YNEPDSNFED PTLAYHPLLS 500
    IYHLVSEMVA RKLAKLQRRQ ALALQAQAQQ RQQQQQVALG TKVALNNNSP 550
    DIMTKMRSPQ KEVVPNPGIF QVPAIGTSGT SNNTNTSNKP PLHVMVPPKL 600
    TIPEQAHTSP TSRKSSDIHT ELNGVLKSTP VPVSGEYQQR SASPVVGEHQ 650
    EKNTIGGIFR RISQSGQSQH PTRQQEPLPE REPPTYMSKS NEISIKVPKS 700
    HSRTISDYIP SARRYPSYVP NSVDVKQKPA KNTTIAPPIR SVSQKQNSDL 750
    PALPQNAELI VQKQRQKLLQ ENLDKLQIND NDNNNVNAVV DGINNDNSDH 800
    YLSVPKGRKL HPSARAKSVG HARRESLKFT RPPIPAALPP SDMTNDNGFL 850
    GEANKERYNP VSSNFSTVPE DSTTYSNDTN NRLTSVYSQE LTEKQILEEA 900
    SKAPPGSMPS IDYPKSMFLK GFFSVQTTSS KPLPIVRHNI ISVLTRMNID 950
    FKEVKGGFIC VQQRPSIETA AVPVITTTGV GLDSGKAMDL QNSLDSQLSS 1000
    SYHSTASSAS RNSSIKRQGS YKRGQNNIPL TPLATNTHQR NSSIPMSPNY 1050
    GNQSNGTSGE LSSMSLDYVQ QQDDILTTSR AQNINNVNGQ TEQTNTSGIK 1100
    ERPPIKFEIH IVKVRIVGLA GVHFKKVSGN TWLYKELASY ILKELNL 1147
    Length:1,147
    Mass (Da):128,338
    Last modified:December 15, 1998 - v3
    Checksum:iAC2660BF3CA69600
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti216 – 2172QH → HD in AAA34723. (PubMed:2957690)Curated
    Sequence conflicti675 – 70733QEPLP…RTISD → SGTYSSKENLQHICQNQMKF PSKYRKAIVVLYQT(PubMed:2957690)CuratedAdd
    BLAST
    Sequence conflicti756 – 7583NAE → KRQ in AAA34723. (PubMed:2957690)Curated
    Sequence conflicti805 – 8051P → PLSVP(PubMed:2957690)Curated
    Sequence conflicti1034 – 10374ATNT → TTNSI(PubMed:2957690)Curated
    Sequence conflicti1041 – 10422NS → KT in AAA34723. (PubMed:2957690)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M69018 Genomic DNA. Translation: AAA34723.1.
    Z73268 Genomic DNA. Translation: CAA97659.1.
    Z73269 Genomic DNA. Translation: CAA97661.1.
    U53876 Genomic DNA. Translation: AAB67540.1.
    BK006945 Genomic DNA. Translation: DAA09412.1.
    PIRiS64930.
    RefSeqiNP_013197.1. NM_001181983.1.

    Genome annotation databases

    EnsemblFungiiYLR096W; YLR096W; YLR096W.
    GeneIDi850785.
    KEGGisce:YLR096W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M69018 Genomic DNA. Translation: AAA34723.1 .
    Z73268 Genomic DNA. Translation: CAA97659.1 .
    Z73269 Genomic DNA. Translation: CAA97661.1 .
    U53876 Genomic DNA. Translation: AAB67540.1 .
    BK006945 Genomic DNA. Translation: DAA09412.1 .
    PIRi S64930.
    RefSeqi NP_013197.1. NM_001181983.1.

    3D structure databases

    ProteinModelPortali P13186.
    SMRi P13186. Positions 93-416.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31369. 153 interactions.
    DIPi DIP-6276N.
    IntActi P13186. 52 interactions.
    MINTi MINT-604572.
    STRINGi 4932.YLR096W.

    Proteomic databases

    MaxQBi P13186.
    PaxDbi P13186.
    PeptideAtlasi P13186.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR096W ; YLR096W ; YLR096W .
    GeneIDi 850785.
    KEGGi sce:YLR096W.

    Organism-specific databases

    CYGDi YLR096w.
    SGDi S000004086. KIN2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000111001.
    HOGENOMi HOG000207352.
    KOi K08286.
    OrthoDBi EOG793BK1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32246-MONOMER.
    BRENDAi 2.7.11.1. 984.

    Miscellaneous databases

    NextBioi 966981.
    PROi P13186.

    Gene expression databases

    Genevestigatori P13186.

    Family and domain databases

    Gene3Di 3.30.310.80. 2 hits.
    InterProi IPR028375. KA1/Ssp2_C.
    IPR001772. KA1_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF02149. KA1. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103243. SSF103243. 2 hits.
    SSF56112. SSF56112. 2 hits.
    PROSITEi PS50032. KA1. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "KIN1 and KIN2 protein kinases localize to the cytoplasmic face of the yeast plasma membrane."
      Tibbetts M., Donovan M., Roe S., Stiltner A.M., Hammond C.I.
      Exp. Cell Res. 213:93-99(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "Characterization of the KIN2 gene product in Saccharomyces cerevisiae and comparison between the kinase activities of p145KIN1 and p145KIN2."
      Donovan M., Romano P., Tibbetts M., Hammond C.I.
      Yeast 10:113-124(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery."
      Elbert M., Rossi G., Brennwald P.
      Mol. Biol. Cell 16:532-549(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SEC9 AND SRO7.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-549; SER-609 AND SER-888, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-549, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND SER-549, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKIN2_YEAST
    AccessioniPrimary (citable) accession number: P13186
    Secondary accession number(s): D6VY96, Q12384
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 146 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3