Serine/threonine-protein kinase KIN2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P13186 (KIN2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 133. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase KIN2
EC=2.7.11.1

Gene names

Name:	KIN2
Ordered Locus Names:	YLR096W
ORF Names:	L8004.3

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine protein kinase involved in the regulation of exocytosis. Induces phosphorylation of SEC9 and its release from the plasma membrane to the cytosol. Ref.5 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with SEC9 and SRO7. Ref.6
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side Ref.4 Ref.6.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Exocytosis
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	exocytosis Inferred from genetic interaction Ref.6. Source: SGD protein autophosphorylation Inferred from direct assay Ref.5. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.4. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCK2	P33306	4	EBI-9723,EBI-3480
RIM15	P43565	2	EBI-9723,EBI-15150

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1147

1147

Serine/threonine-protein kinase KIN2

PRO_0000086133

Regions

Domain

99 – 377

279

Protein kinase

Domain

1098 – 1147

KA1

Nucleotide binding

105 – 113

ATP By similarity

Compositional bias

527 – 536

Poly-Gln

Sites

Active site

248

Proton acceptor By similarity

Binding site

128

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.10

Modified residue

146

Phosphoserine Ref.8 Ref.9

Modified residue

151

Phosphoserine Ref.10

Modified residue

549

Phosphoserine Ref.7 Ref.10

Modified residue

609

Phosphoserine Ref.7 Ref.10

Modified residue

611

Phosphothreonine Ref.8

Modified residue

612

Phosphoserine Ref.10

Modified residue

641

Phosphoserine Ref.9 Ref.10

Modified residue

643

Phosphoserine Ref.9 Ref.10

Modified residue

663

Phosphoserine Ref.10

Modified residue

665

Phosphoserine Ref.9

Modified residue

888

Phosphoserine Ref.7 Ref.10

Experimental info

Sequence conflict

216 – 217

QH → HD in AAA34723. Ref.1

Sequence conflict

675 – 707

QEPLP…RTISD → SGTYSSKENLQHICQNQMKF PSKYRKAIVVLYQT Ref.1

Sequence conflict

756 – 758

NAE → KRQ in AAA34723. Ref.1

Sequence conflict

805

P → PLSVP Ref.1

Sequence conflict

1034 – 1037

ATNT → TTNSI Ref.1

Sequence conflict

1041 – 1042

NS → KT in AAA34723. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P13186 [UniParc].

Last modified December 15, 1998. Version 3.
Checksum: AC2660BF3CA69600
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FASTA

1,147

128,338

        10         20         30         40         50         60 
MPNPNTADYL VNPNFRTSKG GSLSPTPEAF NDTRVAAPAT LRMMGKQSGP RNDQQQAPLM 

        70         80         90        100        110        120 
PPADIKQGKE QAAQRQNDAS RPNGAVELRQ FHRRSLGDWE FLETVGAGSM GKVKLVKHRQ 

       130        140        150        160        170        180 
TKEICVIKIV NRASKAYLHK QHSLPSPKNE SEILERQKRL EKEIARDKRT VREASLGQIL 

       190        200        210        220        230        240 
YHPHICRLFE MCTMSNHFYM LFEYVSGGQL LDYIIQHGSL KEHHARKFAR GIASALQYLH 

       250        260        270        280        290        300 
ANNIVHRDLK IENIMISSSG EIKIIDFGLS NIFDYRKQLH TFCGSLYFAA PELLKAQPYT 

       310        320        330        340        350        360 
GPEVDIWSFG IVLYVLVCGK VPFDDENSSI LHEKIKKGKV DYPSHLSIEV ISLLTRMIVV 

       370        380        390        400        410        420 
DPLRRATLKN VVEHPWMNRG YDFKAPSYVP NRVPLTPEMI DSQVLKEMYR LEFIDDIEDT 

       430        440        450        460        470        480 
RRSLIRLVTE KEYIQLSQEY WDKLSNAKGL SSSLNNNYLN STAQQTLIQN HITSNPSQSG 

       490        500        510        520        530        540 
YNEPDSNFED PTLAYHPLLS IYHLVSEMVA RKLAKLQRRQ ALALQAQAQQ RQQQQQVALG 

       550        560        570        580        590        600 
TKVALNNNSP DIMTKMRSPQ KEVVPNPGIF QVPAIGTSGT SNNTNTSNKP PLHVMVPPKL 

       610        620        630        640        650        660 
TIPEQAHTSP TSRKSSDIHT ELNGVLKSTP VPVSGEYQQR SASPVVGEHQ EKNTIGGIFR 

       670        680        690        700        710        720 
RISQSGQSQH PTRQQEPLPE REPPTYMSKS NEISIKVPKS HSRTISDYIP SARRYPSYVP 

       730        740        750        760        770        780 
NSVDVKQKPA KNTTIAPPIR SVSQKQNSDL PALPQNAELI VQKQRQKLLQ ENLDKLQIND 

       790        800        810        820        830        840 
NDNNNVNAVV DGINNDNSDH YLSVPKGRKL HPSARAKSVG HARRESLKFT RPPIPAALPP 

       850        860        870        880        890        900 
SDMTNDNGFL GEANKERYNP VSSNFSTVPE DSTTYSNDTN NRLTSVYSQE LTEKQILEEA 

       910        920        930        940        950        960 
SKAPPGSMPS IDYPKSMFLK GFFSVQTTSS KPLPIVRHNI ISVLTRMNID FKEVKGGFIC 

       970        980        990       1000       1010       1020 
VQQRPSIETA AVPVITTTGV GLDSGKAMDL QNSLDSQLSS SYHSTASSAS RNSSIKRQGS 

      1030       1040       1050       1060       1070       1080 
YKRGQNNIPL TPLATNTHQR NSSIPMSPNY GNQSNGTSGE LSSMSLDYVQ QQDDILTTSR 

      1090       1100       1110       1120       1130       1140 
AQNINNVNGQ TEQTNTSGIK ERPPIKFEIH IVKVRIVGLA GVHFKKVSGN TWLYKELASY 


ILKELNL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two yeast genes that encode unusual protein kinases." Levin D.E., Hammond C.I., Ralston R.O., Bishop J.M. Proc. Natl. Acad. Sci. U.S.A. 84:6035-6039(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"KIN1 and KIN2 protein kinases localize to the cytoplasmic face of the yeast plasma membrane." Tibbetts M., Donovan M., Roe S., Stiltner A.M., Hammond C.I. Exp. Cell Res. 213:93-99(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Characterization of the KIN2 gene product in Saccharomyces cerevisiae and comparison between the kinase activities of p145KIN1 and p145KIN2." Donovan M., Romano P., Tibbetts M., Hammond C.I. Yeast 10:113-124(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery." Elbert M., Rossi G., Brennwald P. Mol. Biol. Cell 16:532-549(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SEC9 AND SRO7.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-549; SER-609 AND SER-888, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146 AND THR-611, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146; SER-641; SER-643 AND SER-665, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-151; SER-549; SER-609; SER-612; SER-641; SER-643; SER-663 AND SER-888, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M69018 Genomic DNA. Translation: AAA34723.1. Z73268 Genomic DNA. Translation: CAA97659.1. Z73269 Genomic DNA. Translation: CAA97661.1. U53876 Genomic DNA. Translation: AAB67540.1. BK006945 Genomic DNA. Translation: DAA09412.1.
PIR	S64930.
RefSeq	NP_013197.1. NM_001181983.1.
3D structure databases
ProteinModelPortal	P13186.
SMR	P13186. Positions 93-445.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6276N.
IntAct	P13186. 58 interactions.
MINT	MINT-604572.
STRING	4932.YLR096W.
Proteomic databases
PaxDb	P13186.
PeptideAtlas	P13186.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR096W; YLR096W; YLR096W.
GeneID	850785.
KEGG	sce:YLR096W.
Organism-specific databases
CYGD	YLR096w.
SGD	S000004086. KIN2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104353.
HOGENOM	HOG000207352.
KO	K08286.
OrthoDB	EOG4RZ27C.
Enzyme and pathway databases
BRENDA	2.7.11.1. 984.
Gene expression databases
Genevestigator	P13186.
GermOnline	YLR096W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001772. KA1_dom. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF02149. KA1. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF103243. Kinase-assoc_KA1. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS50032. KA1. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966981.

Entry information

Entry name

KIN2_YEAST

Accession

Primary (citable) accession number: P13186
Secondary accession number(s): D6VY96, Q12384

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 133 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents