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Protein

Galactose transporter

Gene

GAL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GAL2 is a facilitated diffusion transporter required for both the high-affinity galactokinase-dependent and low-affinity galactokinase-independent galactose transport processes.

GO - Molecular functioni

  • galactose transmembrane transporter activity Source: SGD
  • glucose transmembrane transporter activity Source: SGD
  • sugar:proton symporter activity Source: GO_Central

GO - Biological processi

  • galactose metabolic process Source: SGD
  • galactose transport Source: SGD
  • glucose import Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32232-MONOMER.
ReactomeiR-SCE-428790. Facilitative Na+-independent glucose transporters.

Protein family/group databases

TCDBi2.A.1.1.6. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose transporter
Alternative name(s):
Galactose permease
Gene namesi
Name:GAL2
Synonyms:IMP1
Ordered Locus Names:YLR081W
ORF Names:L9449.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR081W.
SGDiS000004071. GAL2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 7069CytoplasmicSequence analysisAdd
BLAST
Transmembranei71 – 9121Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini92 – 12130ExtracellularSequence analysisAdd
BLAST
Transmembranei122 – 14221Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini143 – 1497CytoplasmicSequence analysis
Transmembranei150 – 17021Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini171 – 1755ExtracellularSequence analysis
Transmembranei176 – 19621Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini197 – 20711CytoplasmicSequence analysisAdd
BLAST
Transmembranei208 – 22821Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini229 – 24214ExtracellularSequence analysisAdd
BLAST
Transmembranei243 – 26321Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini264 – 34279CytoplasmicSequence analysisAdd
BLAST
Transmembranei343 – 36220Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini363 – 3664ExtracellularSequence analysis
Transmembranei367 – 38721Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini388 – 3947CytoplasmicSequence analysis
Transmembranei395 – 41521Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini416 – 43520ExtracellularSequence analysisAdd
BLAST
Transmembranei436 – 45621Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini457 – 47216CytoplasmicSequence analysisAdd
BLAST
Transmembranei473 – 49321Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini494 – 4996ExtracellularSequence analysis
Transmembranei500 – 52021Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini521 – 57454CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 574573Galactose transporterPRO_0000050416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321PhosphoserineCombined sources
Modified residuei35 – 351PhosphoserineCombined sources
Modified residuei39 – 391PhosphoserineCombined sources
Modified residuei48 – 481PhosphoserineCombined sources
Modified residuei50 – 501PhosphoserineCombined sources
Modified residuei53 – 531PhosphoserineCombined sources
Modified residuei55 – 551PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13181.

PTM databases

iPTMnetiP13181.

Interactioni

Protein-protein interaction databases

BioGridi31354. 41 interactions.
DIPiDIP-4773N.
IntActiP13181. 1 interaction.
MINTiMINT-569136.

Structurei

3D structure databases

ProteinModelPortaliP13181.
SMRiP13181. Positions 74-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
InParanoidiP13181.
KOiK08139.
OrthoDBiEOG73JM4H.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13181-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVEENNMPV VSQQPQAGED VISSLSKDSH LSAQSQKYSN DELKAGESGS
60 70 80 90 100
EGSQSVPIEI PKKPMSEYVT VSLLCLCVAF GGFMFGWDTG TISGFVVQTD
110 120 130 140 150
FLRRFGMKHK DGTHYLSNVR TGLIVAIFNI GCAFGGIILS KGGDMYGRKK
160 170 180 190 200
GLSIVVSVYI VGIIIQIASI NKWYQYFIGR IISGLGVGGI AVLCPMLISE
210 220 230 240 250
IAPKHLRGTL VSCYQLMITA GIFLGYCTNY GTKSYSNSVQ WRVPLGLCFA
260 270 280 290 300
WSLFMIGALT LVPESPRYLC EVNKVEDAKR SIAKSNKVSP EDPAVQAELD
310 320 330 340 350
LIMAGIEAEK LAGNASWGEL FSTKTKVFQR LLMGVFVQMF QQLTGNNYFF
360 370 380 390 400
YYGTVIFKSV GLDDSFETSI VIGVVNFAST FFSLWTVENL GHRKCLLLGA
410 420 430 440 450
ATMMACMVIY ASVGVTRLYP HGKSQPSSKG AGNCMIVFTC FYIFCYATTW
460 470 480 490 500
APVAWVITAE SFPLRVKSKC MALASASNWV WGFLIAFFTP FITSAINFYY
510 520 530 540 550
GYVFMGCLVA MFFYVFFFVP ETKGLSLEEI QELWEEGVLP WKSEGWIPSS
560 570
RRGNNYDLED LQHDDKPWYK AMLE
Length:574
Mass (Da):63,626
Last modified:December 15, 1998 - v3
Checksum:i5ACB68A4094F01A5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501S → P (PubMed:2666404).Curated
Sequence conflicti50 – 501S → P (PubMed:2697639).Curated
Sequence conflicti79 – 791A → R in AAA34623 (PubMed:2666404).Curated
Sequence conflicti90 – 901G → S (PubMed:2666404).Curated
Sequence conflicti90 – 901G → S (PubMed:2697639).Curated
Sequence conflicti392 – 3921H → R (PubMed:2666404).Curated
Sequence conflicti392 – 3921H → R (PubMed:2697639).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68547 Genomic DNA. Translation: AAA34623.1.
M81879 Genomic DNA. Translation: AAA34624.1.
Z73253 Genomic DNA. Translation: CAA97640.1.
Z73254 Genomic DNA. Translation: CAA97642.1.
U53880 Genomic DNA. Translation: AAB67585.1.
BK006945 Genomic DNA. Translation: DAA09397.1.
PIRiS64913.
RefSeqiNP_013182.1. NM_001181968.1.

Genome annotation databases

EnsemblFungiiYLR081W; YLR081W; YLR081W.
GeneIDi850770.
KEGGisce:YLR081W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68547 Genomic DNA. Translation: AAA34623.1.
M81879 Genomic DNA. Translation: AAA34624.1.
Z73253 Genomic DNA. Translation: CAA97640.1.
Z73254 Genomic DNA. Translation: CAA97642.1.
U53880 Genomic DNA. Translation: AAB67585.1.
BK006945 Genomic DNA. Translation: DAA09397.1.
PIRiS64913.
RefSeqiNP_013182.1. NM_001181968.1.

3D structure databases

ProteinModelPortaliP13181.
SMRiP13181. Positions 74-535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31354. 41 interactions.
DIPiDIP-4773N.
IntActiP13181. 1 interaction.
MINTiMINT-569136.

Protein family/group databases

TCDBi2.A.1.1.6. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP13181.

Proteomic databases

MaxQBiP13181.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR081W; YLR081W; YLR081W.
GeneIDi850770.
KEGGisce:YLR081W.

Organism-specific databases

EuPathDBiFungiDB:YLR081W.
SGDiS000004071. GAL2.

Phylogenomic databases

GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
InParanoidiP13181.
KOiK08139.
OrthoDBiEOG73JM4H.

Enzyme and pathway databases

BioCyciYEAST:G3O-32232-MONOMER.
ReactomeiR-SCE-428790. Facilitative Na+-independent glucose transporters.

Miscellaneous databases

PROiP13181.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and structure of the yeast galactose transporter."
    Szkutnicka K., Tschopp J.F., Andrews L., Cirillo V.P.
    J. Bacteriol. 171:4486-4493(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Yeast galactose permease is related to yeast and mammalian glucose transporters."
    Nehlin J.O., Carlberg M., Ronne H.
    Gene 85:313-319(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-35; SER-39; SER-48; SER-50; SER-53 AND SER-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGAL2_YEAST
AccessioniPrimary (citable) accession number: P13181
Secondary accession number(s): D6VY81, Q12521
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 15, 1998
Last modified: July 6, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.