Reviewed, UniProtKB/Swiss-Prot P13179 (L_CHAV)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Chandipura virus (strain I653514) (CHPV)

Taxonomic identifier

11273 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Vesiculovirus

Virus host

Phlebotominae (sandflies) [TaxID: 7198]
Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2092 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	VirionPotential. CytoplasmBy similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2092	2092	Large structural protein		PRO_0000222839
Regions
	Domain	588 – 774	187	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P13179-1 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: AE9091D6CFF0B206	FASTA	2,092	238,532
	10 20 30 40 50 60 MDLNPVDDAA ELSEENFFSG KLSKECRIRG LNSVDYNLNS PLVSDDLTYL LDKFKGKPVP 70 80 90 100 110 120 IRWKMKKWDS ILDQLRKHDL EYLRPSDLHQ WFAEWMLYSK HGSKQGEDFL KTVDEEASDT 130 140 150 160 170 180 FEVVRSFIRG WTGGEINFVR KSGKHMGYCA ELCQKFLDLH KLTLLGNAAT DNELLQLSKT 190 200 210 220 230 240 FGDDKIYKKR LIKLPSLGRV IFDSGFFIVL DQRVLMDRNF MLMMKDVIIG RMQTVLSMIS 250 260 270 280 290 300 RCDDKFSSKD IDFLLKVYST GDKIIRKLGN DGYELIKTVE PMCNLRLSDL ARRFRPLIPP 310 320 330 340 350 360 FPHFRRHIES TVDELSAKTP LIRELFSLID TSPNVDSTLV VYGSFRHWGH PFINYFEGLE 370 380 390 400 410 420 KLHKQVTMEK EIDTNYSEAL ASDLARIVLT KEFNEKKQWA VDYHRVPTNH PFKNHIRDNT 430 440 450 460 470 480 WPTAAVIQDF GDHWHELPLI QCFDIPDLID PSIIYSDKSH SMNRSEVLNH VRTKPHTPIP 490 500 510 520 530 540 SKKVLESMID KPATNWLEFL EEIDKNGLSD EDLVIGLKGK ERELKIAGRF FSLMSWKLRE 550 560 570 580 590 600 YFVVTEYLIK THFVPLFHGL TMADDMTAVI KKMLESSSGQ GLTNYESVCI ANHIDYEKWN 610 620 630 640 650 660 NHQRKLSNGP VFKVMGQFLG YPNLIYRTHE FFEKSLIYYN ERPDLMKVKN GILENSTHQR 670 680 690 700 710 720 VCWNGQAGGL EGLRQKGWSI LNLLVIQREA KIRNTAVKVL AQGDNQVICT QYKTKQYRND 730 740 750 760 770 780 IELRQALNQM AANNDVIMKA IESGTNKLGL LINQDETMQS ADYLNYGKVP IFRGVIRGLE 790 800 810 820 830 840 TKRWSRVTCV TNDQLPTCAN LMSSVSTNAL TVAHFDVHPL NAMIQFNFFG NFARLLLIMH 850 860 870 880 890 900 DPAIRQSLNQ LKGPNINVHS YGFKVAMLYL DPSIGGVCGT ALSRFLIRSF PDPVTESLSF 910 920 930 940 950 960 WKLIHHSTSD IRLKNLSEQF GNPKIAVFRE SHIEKLLEDP TSLNISMGMS AANLLKTEIK 970 980 990 1000 1010 1020 KNLLQKKSSI GNQIVKDAVY YIHSEDEKLR TFLWSITPLF PRFLSEFKAG TFMGVASSIV 1030 1040 1050 1060 1070 1080 SLFQNSRTIR NVFRDYMSQT IDDLIVKSEL TSLEHLSNYT DRKGSGGIWG CSAEQADKLR 1090 1100 1110 1120 1130 1140 RMSWKRPVLG TTVPHPLEMH GRGTLKSPLS KCCKESRMDY ISVHIPEGLN KVLDGRGSLP 1150 1160 1170 1180 1190 1200 AYLGSKTSES TSILQPWEKE SKIPIIRRAT RLRDAIHWFV DPDSNLARSI LNNIESLTGE 1210 1220 1230 1240 1250 1260 KWEGALKGYK RTGSALHRFS TSRVSHGGFS SQSPACLTRM MATTDTMRDY AQLNYDFMFQ 1270 1280 1290 1300 1310 1320 ASLLYSQMTS SVILMGTTVS NTIHFHVTCR KCIREITEPM LESPREYRGK DVHLVLAKWK 1330 1340 1350 1360 1370 1380 NSSNGWGETL QLLKPVEGDW DTIPPVEKSY HVGRILGFLY GDLKSQNSSR ADDSSIFPLS 1390 1400 1410 1420 1430 1440 IQMRLRGRGF LRGILDGLVR ASACQVIHRR SVALLSKPAN AIYGGLIYLI DKISASTSFT 1450 1460 1470 1480 1490 1500 TLCRDGPIRE ELSSIPHKIP TSYPTSNSDM GLHIRNYLKF QCKTVELGKY QSDIKDLWLF 1510 1520 1530 1540 1550 1560 SDVMTSNIAG PFALSTKILK CLYKPALSQK DRNNIRKISN FSKMMRSQLS WDPTSSEFIT 1570 1580 1590 1600 1610 1620 SQILVCNEEI RHACKFGIPK LSLKFDDPVW GPEDYGLIWS IPVDYSSQSV PKNLKPCPRI 1630 1640 1650 1660 1670 1680 QNPSISGFRL GQLPTGAHYK LRSILRKKNI HYRDALCGGD GSGGMTAAVL RYNLKARAIF 1690 1700 1710 1720 1730 1740 NSILDFDGST MKGASPDPPS ALETVVNGRT RCVNAESCWE NPSDLSEQRT WDYFKFLKTH 1750 1760 1770 1780 1790 1800 HGLKIDLIVL DMEVRDFAIS ASIEKCVRHN VSSILEEDGV LIYKTYGSTI AAESSNAVVN 1810 1820 1830 1840 1850 1860 IGVLFESVEL IQTEYSSTST SEVYMYCRKI KKFVDAPHPD WISLDYYWSK LLCFRSYKEE 1870 1880 1890 1900 1910 1920 FFRSYEVSRK ESLKGIPNSF IPDPLVNLET LLQIAGVPSG ISHQLAIDIK ESQLTQITAA 1930 1940 1950 1960 1970 1980 MVLCGMIANY TLDVTKKRDS YNPPSDGRLI RMSAALVGIS FWISVKYYDK ELNFELEQIL 1990 2000 2010 2020 2030 2040 SRSFPIRWML SHNYLFWTTK KGFRNAKDVR LSGNMANIGN WIRCMELLHL PPGSLSKDEV 2050 2060 2070 2080 2090 TTTCGKYIRN LKYSVILQQT GIIDLWKSRV ASDDRSLMEV KTEFIESEHW VD

References

[1]	"Complete genome sequences of Chandipura and Isfahan vesiculoviruses." Marriott A.C. Arch. Virol. 150:671-680(2005) [PubMed: 15614433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Structure and expression of the glycoprotein gene of Chandipura virus." Masters P.S., Bhella R.S., Butcher M., Patel B., Ghosh H.P., Banerjee A.K. Virology 171:285-290(1989) [PubMed: 2741347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ810083 Genomic RNA. Translation: CAH17543.1. J04350 Genomic RNA. Translation: AAA42917.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017234. RNA-dir_RNA_pol_rhabdovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_CHAV

Accession

Primary (citable) accession number: P13179
Secondary accession number(s): Q5K2K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	May 15, 2007
Last modified:	November 25, 2008
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)