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Protein

Interferon-induced transmembrane protein 1

Gene

IFITM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation.8 Publications

GO - Molecular functioni

  • receptor signaling protein activity Source: ProtInc

GO - Biological processi

  • cell surface receptor signaling pathway Source: ProtInc
  • cytokine-mediated signaling pathway Source: Reactome
  • defense response to virus Source: UniProtKB-KW
  • intracellular signal transduction Source: GOC
  • negative regulation of cell migration Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • negative regulation of viral entry into host cell Source: UniProtKB
  • negative regulation of viral genome replication Source: UniProtKB
  • ossification Source: UniProtKB-KW
  • positive regulation of osteoblast differentiation Source: UniProtKB
  • regulation of immune response Source: Reactome
  • response to interferon-alpha Source: UniProtKB
  • response to interferon-beta Source: UniProtKB
  • response to interferon-gamma Source: UniProtKB
  • response to virus Source: UniProtKB
  • type I interferon signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Osteogenesis

Enzyme and pathway databases

ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
REACT_25162. Interferon alpha/beta signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-induced transmembrane protein 1
Alternative name(s):
Dispanin subfamily A member 2a
Short name:
DSPA2a
Interferon-induced protein 17
Interferon-inducible protein 9-27
Leu-13 antigen
CD_antigen: CD225
Gene namesi
Name:IFITM1
Synonyms:CD225, IFI17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:5412. IFITM1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3636CytoplasmicSequence AnalysisAdd
BLAST
Intramembranei37 – 5721Helical1 PublicationAdd
BLAST
Topological domaini58 – 8629CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei87 – 10721HelicalSequence AnalysisAdd
BLAST
Topological domaini108 – 12518ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • membrane Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29653.

Polymorphism and mutation databases

BioMutaiIFITM1.
DMDMi215274118.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 125125Interferon-induced transmembrane protein 1PRO_0000153727Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi50 – 501S-palmitoyl cysteineBy similarity
Lipidationi51 – 511S-palmitoyl cysteineBy similarity
Lipidationi84 – 841S-palmitoyl cysteineBy similarity

Post-translational modificationi

Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.By similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

MaxQBiP13164.
PaxDbiP13164.
PeptideAtlasiP13164.
PRIDEiP13164.

PTM databases

PhosphoSiteiP13164.

Expressioni

Tissue specificityi

Bone (at protein level). Levels greatly elevated in colon cancer, cervical cancer, esophageal cancer and ovarian cancer. Expressed in glioma cell lines.2 Publications

Inductioni

By IFN-alpha and IFNG/IFN-gamma.

Gene expression databases

BgeeiP13164.
CleanExiHS_IFITM1.
GenevisibleiP13164. HS.

Organism-specific databases

HPAiCAB017615.
HPA004810.

Interactioni

Subunit structurei

Interacts with CAV1; this interaction enhances the ability of CAV1 in inhibiting ERK activation. Interacts with CD81.2 Publications

Protein-protein interaction databases

BioGridi114091. 5 interactions.
DIPiDIP-32672N.
IntActiP13164. 4 interactions.
MINTiMINT-1036538.
STRINGi9606.ENSP00000330825.

Structurei

3D structure databases

ProteinModelPortaliP13164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni84 – 12542Interaction with CAV1Add
BLAST

Sequence similaritiesi

Belongs to the CD225/Dispanin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG268489.
GeneTreeiENSGT00390000003476.
HOGENOMiHOG000115781.
HOVERGENiHBG001182.
InParanoidiP13164.
KOiK06566.
OMAiWCCLGFI.
OrthoDBiEOG7288T5.
PhylomeDBiP13164.
TreeFamiTF334894.

Family and domain databases

InterProiIPR007593. CD225/Dispanin_fam.
[Graphical view]
PfamiPF04505. Dispanin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKEEHEVAV LGPPPSTILP RSTVINIHSE TSVPDHVVWS LFNTLFLNWC
60 70 80 90 100
CLGFIAFAYS VKSRDRKMVG DVTGAQAYAS TAKCLNIWAL ILGILMTIGF
110 120
ILLLVFGSVT VYHIMLQIIQ EKRGY
Length:125
Mass (Da):13,964
Last modified:November 25, 2008 - v3
Checksum:i10EE5B64894838ED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031L → S in AAA35494 (PubMed:2492664).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti13 – 131P → A.7 Publications
Corresponds to variant rs9667990 [ dbSNP | Ensembl ].
VAR_047422

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04164 mRNA. Translation: AAA35494.1.
X84958 mRNA. Translation: CAA59337.1.
BT007173 mRNA. Translation: AAP35837.1.
AK290480 mRNA. Translation: BAF83169.1.
AC136475 Genomic DNA. No translation available.
CH471278 Genomic DNA. Translation: EAW61218.1.
BC000897 mRNA. Translation: AAH00897.1.
CCDSiCCDS41584.1.
PIRiA31454.
RefSeqiNP_003632.3. NM_003641.3.
UniGeneiHs.458414.

Genome annotation databases

EnsembliENST00000328221; ENSP00000330825; ENSG00000185885.
ENST00000408968; ENSP00000386187; ENSG00000185885.
ENST00000528780; ENSP00000437057; ENSG00000185885.
GeneIDi8519.
KEGGihsa:8519.
UCSCiuc001loy.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04164 mRNA. Translation: AAA35494.1.
X84958 mRNA. Translation: CAA59337.1.
BT007173 mRNA. Translation: AAP35837.1.
AK290480 mRNA. Translation: BAF83169.1.
AC136475 Genomic DNA. No translation available.
CH471278 Genomic DNA. Translation: EAW61218.1.
BC000897 mRNA. Translation: AAH00897.1.
CCDSiCCDS41584.1.
PIRiA31454.
RefSeqiNP_003632.3. NM_003641.3.
UniGeneiHs.458414.

3D structure databases

ProteinModelPortaliP13164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114091. 5 interactions.
DIPiDIP-32672N.
IntActiP13164. 4 interactions.
MINTiMINT-1036538.
STRINGi9606.ENSP00000330825.

PTM databases

PhosphoSiteiP13164.

Polymorphism and mutation databases

BioMutaiIFITM1.
DMDMi215274118.

Proteomic databases

MaxQBiP13164.
PaxDbiP13164.
PeptideAtlasiP13164.
PRIDEiP13164.

Protocols and materials databases

DNASUi8519.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328221; ENSP00000330825; ENSG00000185885.
ENST00000408968; ENSP00000386187; ENSG00000185885.
ENST00000528780; ENSP00000437057; ENSG00000185885.
GeneIDi8519.
KEGGihsa:8519.
UCSCiuc001loy.4. human.

Organism-specific databases

CTDi8519.
GeneCardsiGC11P000313.
HGNCiHGNC:5412. IFITM1.
HPAiCAB017615.
HPA004810.
MIMi604456. gene.
neXtProtiNX_P13164.
PharmGKBiPA29653.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG268489.
GeneTreeiENSGT00390000003476.
HOGENOMiHOG000115781.
HOVERGENiHBG001182.
InParanoidiP13164.
KOiK06566.
OMAiWCCLGFI.
OrthoDBiEOG7288T5.
PhylomeDBiP13164.
TreeFamiTF334894.

Enzyme and pathway databases

ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
REACT_25162. Interferon alpha/beta signaling.

Miscellaneous databases

ChiTaRSiIFITM1. human.
GeneWikiiIFITM1.
GenomeRNAii8519.
NextBioi31894.
PROiP13164.
SOURCEiSearch...

Gene expression databases

BgeeiP13164.
CleanExiHS_IFITM1.
GenevisibleiP13164. HS.

Family and domain databases

InterProiIPR007593. CD225/Dispanin_fam.
[Graphical view]
PfamiPF04505. Dispanin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A single DNA response element can confer inducibility by both alpha- and gamma-interferons."
    Reid L.E., Brasnett A.H., Gilbert C.S., Porter A.C.G., Gewert D.R., Stark G.R., Kerr I.M.
    Proc. Natl. Acad. Sci. U.S.A. 86:840-844(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-13.
  2. "Expression cloning of an interferon-inducible 17-kDa membrane protein implicated in the control of cell growth."
    Deblandre G.A., Marinx O.P., Evans S.S., Majjaj S., Leo O., Caput D., Huez G.A., Wathelet M.G.
    J. Biol. Chem. 270:23860-23866(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-13.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-13.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-13.
    Tissue: Brain.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-13.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-13.
    Tissue: Cervix.
  8. "TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen."
    Takahashi S., Doss C., Levy S., Levy R.
    J. Immunol. 145:2207-2213(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CD81.
  9. "IFITM1 plays an essential role in the antiproliferative action of interferon-gamma."
    Yang G., Xu Y., Chen X., Hu G.
    Oncogene 26:594-603(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Binding of IFITM1 enhances the inhibiting effect of caveolin-1 on ERK activation."
    Xu Y., Yang G., Hu G.
    Acta Biochim. Biophys. Sin. 41:488-494(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CAV1.
  11. "The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus."
    Brass A.L., Huang I.C., Benita Y., John S.P., Krishnan M.N., Feeley E.M., Ryan B.J., Weyer J.L., van der Weyden L., Fikrig E., Adams D.J., Xavier R.J., Farzan M., Elledge S.J.
    Cell 139:1243-1254(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN VIRAL RESISTANCE.
  12. "The small interferon-induced transmembrane genes and proteins."
    Siegrist F., Ebeling M., Certa U.
    J. Interferon Cytokine Res. 31:183-197(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  13. "Knockdown of interferon-induced transmembrane protein 1 (IFITM1) inhibits proliferation, migration, and invasion of glioma cells."
    Yu F., Ng S.S., Chow B.K., Sze J., Lu G., Poon W.S., Kung H.F., Lin M.C.
    J. Neurooncol. 103:187-195(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  14. "The IFITM proteins inhibit HIV-1 infection."
    Lu J., Pan Q., Rong L., He W., Liu S.L., Liang C.
    J. Virol. 85:2126-2137(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. Erratum
    Lu J., Pan Q., Rong L., He W., Liu S.L., Liang C.
    J. Virol. 85:4043-4043(2011)
  16. "ISG56 and IFITM1 proteins inhibit hepatitis C virus replication."
    Raychoudhuri A., Shrivastava S., Steele R., Kim H., Ray R., Ray R.B.
    J. Virol. 85:12881-12889(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. Cited for: FUNCTION.
  18. "IFITM1 increases osteogenesis through Runx2 in human alveolar-derived bone marrow stromal cells."
    Kim B.S., Kim H.J., Kim J.S., You Y.O., Zadeh H., Shin H.I., Lee S.J., Park Y.J., Takata T., Pi S.H., Lee J., You H.K.
    Bone 51:506-514(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  19. "IFITM proteins restrict antibody-dependent enhancement of dengue virus infection."
    Chan Y.K., Huang I.C., Farzan M.
    PLoS ONE 7:E34508-E34508(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  20. "The dispanins: a novel gene family of ancient origin that contains 14 human members."
    Sallman Almen M., Bringeland N., Fredriksson R., Schioth H.B.
    PLoS ONE 7:E31961-E31961(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  21. "A membrane topology model for human interferon inducible transmembrane protein 1."
    Weston S., Czieso S., White I.J., Smith S.E., Kellam P., Marsh M.
    PLoS ONE 9:E104341-E104341(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
  22. Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-13, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIFM1_HUMAN
AccessioniPrimary (citable) accession number: P13164
Secondary accession number(s): Q15322, Q53XZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 25, 2008
Last modified: June 24, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.