P13164 (IFM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon-induced transmembrane protein 1

Alternative name(s):
Dispanin subfamily A member 2a
Short name=DSPA2a
Interferon-induced protein 17
Interferon-inducible protein 9-27
Leu-13 antigen
CD_antigen=CD225

Gene names

Name:	IFITM1
Synonyms:	CD225, IFI17

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation. Ref.9 Ref.11 Ref.13 Ref.14 Ref.16 Ref.17 Ref.18 Ref.19
Subunit structure	Interacts with CAV1; this interaction enhances the ability of CAV1 in inhibiting ERK activation. Interacts with CD81. Ref.8 Ref.10
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.10.
Tissue specificity	Bone (at protein level). Levels greatly elevated in colon cancer, cervical cancer, esophageal cancer and ovarian cancer. Expressed in glioma cell lines. Ref.13 Ref.18
Induction	By IFN-alpha and IFNG/IFN-gamma.
Post-translational modification	Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus By similarity.
Sequence similarities	Belongs to the CD225/Dispanin family.

Ontologies

Keywords
Biological process	Antiviral defense Immunity Innate immunity Osteogenesis
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell migration Inferred from mutant phenotype Ref.13. Source: UniProtKB negative regulation of cell proliferation Inferred from mutant phenotype Ref.13. Source: UniProtKB negative regulation of viral entry into host cell Inferred from direct assay Ref.17. Source: UniProtKB negative regulation of viral genome replication Inferred from direct assay Ref.17. Source: UniProtKB ossification Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of osteoblast differentiation Inferred from mutant phenotype Ref.18. Source: UniProtKB regulation of immune response Traceable author statement. Source: Reactome response to interferon-alpha Inferred from direct assay Ref.19. Source: UniProtKB response to interferon-beta Inferred from direct assay Ref.17. Source: UniProtKB response to interferon-gamma Inferred from direct assay Ref.17. Source: UniProtKB response to virus Inferred from direct assay Ref.17Ref.19. Source: UniProtKB type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	receptor signaling protein activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABHD16A	O95870	1	EBI-348505,EBI-348517

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 125	125	Interferon-induced transmembrane protein 1		PRO_0000153727
Regions
	Topological domain	1 – 36	36	Extracellular Potential
	Transmembrane	37 – 57	21	Helical; Potential
	Topological domain	58 – 86	29	Cytoplasmic Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Topological domain	108 – 125	18	Extracellular Potential
	Region	84 – 125	42	Interaction with CAV1
Amino acid modifications
	Lipidation	50	1	S-palmitoyl cysteine By similarity
	Lipidation	51	1	S-palmitoyl cysteine By similarity
	Lipidation	84	1	S-palmitoyl cysteine By similarity
Natural variations
	Natural variant	13	1	P → A. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7 Ref.21 Corresponds to variant rs9667990 [ dbSNP | Ensembl ].		VAR_047422
Experimental info
	Sequence conflict	103	1	L → S in AAA35494. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13164 [UniParc]. Last modified November 25, 2008. Version 3. Checksum: 10EE5B64894838ED	FASTA	125	13,964
	10 20 30 40 50 60 MHKEEHEVAV LGPPPSTILP RSTVINIHSE TSVPDHVVWS LFNTLFLNWC CLGFIAFAYS 70 80 90 100 110 120 VKSRDRKMVG DVTGAQAYAS TAKCLNIWAL ILGILMTIGF ILLLVFGSVT VYHIMLQIIQ EKRGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A single DNA response element can confer inducibility by both alpha- and gamma-interferons." Reid L.E., Brasnett A.H., Gilbert C.S., Porter A.C.G., Gewert D.R., Stark G.R., Kerr I.M. Proc. Natl. Acad. Sci. U.S.A. 86:840-844(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-13.
[2]	"Expression cloning of an interferon-inducible 17-kDa membrane protein implicated in the control of cell growth." Deblandre G.A., Marinx O.P., Evans S.S., Majjaj S., Leo O., Caput D., Huez G.A., Wathelet M.G. J. Biol. Chem. 270:23860-23866(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-13.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-13.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-13. Tissue: Brain.
[5]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-13.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-13. Tissue: Cervix.
[8]	"TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen." Takahashi S., Doss C., Levy S., Levy R. J. Immunol. 145:2207-2213(1990) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CD81.
[9]	"IFITM1 plays an essential role in the antiproliferative action of interferon-gamma." Yang G., Xu Y., Chen X., Hu G. Oncogene 26:594-603(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Binding of IFITM1 enhances the inhibiting effect of caveolin-1 on ERK activation." Xu Y., Yang G., Hu G. Acta Biochim. Biophys. Sin. 41:488-494(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CAV1.
[11]	"The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus." Brass A.L., Huang I.C., Benita Y., John S.P., Krishnan M.N., Feeley E.M., Ryan B.J., Weyer J.L., van der Weyden L., Fikrig E., Adams D.J., Xavier R.J., Farzan M., Elledge S.J. Cell 139:1243-1254(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN VIRAL RESISTANCE.
[12]	"The small interferon-induced transmembrane genes and proteins." Siegrist F., Ebeling M., Certa U. J. Interferon Cytokine Res. 31:183-197(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[13]	"Knockdown of interferon-induced transmembrane protein 1 (IFITM1) inhibits proliferation, migration, and invasion of glioma cells." Yu F., Ng S.S., Chow B.K., Sze J., Lu G., Poon W.S., Kung H.F., Lin M.C. J. Neurooncol. 103:187-195(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[14]	"The IFITM proteins inhibit HIV-1 infection." Lu J., Pan Q., Rong L., He W., Liu S.L., Liang C. J. Virol. 85:2126-2137(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	Erratum Lu J., Pan Q., Rong L., He W., Liu S.L., Liang C. J. Virol. 85:4043-4043(2011)
[16]	"ISG56 and IFITM1 proteins inhibit hepatitis C virus replication." Raychoudhuri A., Shrivastava S., Steele R., Kim H., Ray R., Ray R.B. J. Virol. 85:12881-12889(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[17]	"Distinct patterns of IFITM-mediated restriction of filoviruses, SARS coronavirus, and influenza A virus." Huang I.C., Bailey C.C., Weyer J.L., Radoshitzky S.R., Becker M.M., Chiang J.J., Brass A.L., Ahmed A.A., Chi X., Dong L., Longobardi L.E., Boltz D., Kuhn J.H., Elledge S.J., Bavari S., Denison M.R., Choe H., Farzan M. PLoS Pathog. 7:E1001258-E1001258(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[18]	"IFITM1 increases osteogenesis through Runx2 in human alveolar-derived bone marrow stromal cells." Kim B.S., Kim H.J., Kim J.S., You Y.O., Zadeh H., Shin H.I., Lee S.J., Park Y.J., Takata T., Pi S.H., Lee J., You H.K. Bone 51:506-514(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[19]	"IFITM proteins restrict antibody-dependent enhancement of dengue virus infection." Chan Y.K., Huang I.C., Farzan M. PLoS ONE 7:E34508-E34508(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[20]	"The dispanins: a novel gene family of ancient origin that contains 14 human members." Sallman Almen M., Bringeland N., Fredriksson R., Schioth H.B. PLoS ONE 7:E31961-E31961(2012) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[21]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-13, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04164 mRNA. Translation: AAA35494.1. X84958 mRNA. Translation: CAA59337.1. BT007173 mRNA. Translation: AAP35837.1. AK290480 mRNA. Translation: BAF83169.1. AC136475 Genomic DNA. No translation available. CH471278 Genomic DNA. Translation: EAW61218.1. BC000897 mRNA. Translation: AAH00897.1.
IPI	IPI00300620.
PIR	A31454.
RefSeq	NP_003632.3. NM_003641.3.
UniGene	Hs.458414.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-32672N.
IntAct	P13164. 3 interactions.
MINT	MINT-1036538.
STRING	9606.ENSP00000386187.
PTM databases
PhosphoSite	P13164.
Polymorphism databases
DMDM	215274118.
Proteomic databases
PaxDb	P13164.
PeptideAtlas	P13164.
PRIDE	P13164.
Protocols and materials databases
DNASU	8519.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000328221; ENSP00000330825; ENSG00000185885. ENST00000408968; ENSP00000386187; ENSG00000185885. ENST00000528780; ENSP00000437057; ENSG00000185885.
GeneID	8519.
KEGG	hsa:8519.
Organism-specific databases
CTD	8519.
GeneCards	GC11P000313.
HGNC	HGNC:5412. IFITM1.
HPA	CAB017615. HPA004810.
MIM	604456. gene.
neXtProt	NX_P13164.
PharmGKB	PA29653.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG268489.
HOGENOM	HOG000115781.
HOVERGEN	HBG001182.
InParanoid	P13164.
KO	K06566.
OMA	WCCLGFI.
PhylomeDB	P13164.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
Bgee	P13164.
CleanEx	HS_IFITM1.
Genevestigator	P13164.
GermOnline	ENSG00000185885. Homo sapiens.
Family and domain databases
InterPro	IPR007593. Interferon-induced_TM_protein. [Graphical view]
Pfam	PF04505. CD225. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	IFITM1. human.
GenomeRNAi	8519.
NextBio	31894.
SOURCE	Search...

Entry information

Entry name

IFM1_HUMAN

Accession

Primary (citable) accession number: P13164
Secondary accession number(s): Q15322, Q53XZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 25, 2008
Last modified:	April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.