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Protein

Thymidine kinase

Gene

TK

Organism
Feline herpesvirus 1 (FeHV-1) (Feline viral rhinotracheitis virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei56Proton acceptorUniRule annotation1
Binding sitei74SubstrateUniRule annotation1
Binding sitei98SubstrateUniRule annotation1
Binding sitei188ATPUniRule annotation1
Binding sitei194SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
OrganismiFeline herpesvirus 1 (FeHV-1) (Feline viral rhinotracheitis virus)
Taxonomic identifieri10334 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiFelidae (cat family) [TaxID: 9681]

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1795128.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001750751 – 343Thymidine kinaseAdd BLAST343

Proteomic databases

PRIDEiP13159.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Chemistry databases

BindingDBiP13159.

Structurei

3D structure databases

ProteinModelPortaliP13159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P13159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGTIPVQN EEIIKSQVNT VRIYIDGAYG IGKSLTAKYL VRADENRPGY
60 70 80 90 100
TYYFPEPMLY WRSLFETDVV GGIYAVQDRK RRGELSAEDA AYITAHYQAR
110 120 130 140 150
FAAPYLLLHS RLSTITGYQK VVCEEHPDVT LIIDRHPLAS LVCFPLARYF
160 170 180 190 200
VGDMTLGSVL SLMATLPREP PGGNLVVTTL NIEEHLKRLR GRSRTGEQID
210 220 230 240 250
MKLIHALRNV YMMLVHTKKF LTKNTSWRDG WGKLKIFSHY ERNRLVETTI
260 270 280 290 300
VSDSTESDLC DTLFSVFKAR ELSDQNGDLL DMHAWVLDGL METLQNLQIF
310 320 330 340
TLNLEGTPDE CAAALGALRQ DMDMTFIAAC DMHRISEALT IYH
Length:343
Mass (Da):38,923
Last modified:January 1, 1990 - v1
Checksum:i26B1A771E3AD1260
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26660 Genomic DNA. Translation: AAA46172.1.
PIRiA32388. KIBEFH.
RefSeqiYP_003331558.1. NC_013590.2.

Genome annotation databases

GeneIDi8658565.
KEGGivg:8658565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26660 Genomic DNA. Translation: AAA46172.1.
PIRiA32388. KIBEFH.
RefSeqiYP_003331558.1. NC_013590.2.

3D structure databases

ProteinModelPortaliP13159.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP13159.
ChEMBLiCHEMBL1795128.

Proteomic databases

PRIDEiP13159.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi8658565.
KEGGivg:8658565.

Miscellaneous databases

PROiP13159.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_FHV1
AccessioniPrimary (citable) accession number: P13159
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 5, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.