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Protein

Calpain small subunit 1

Gene

CAPNS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi104Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi107Calcium 1By similarity1
Metal bindingi109Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi114Calcium 1By similarity1
Metal bindingi132Calcium 4By similarity1
Metal bindingi147Calcium 2By similarity1
Metal bindingi149Calcium 2By similarity1
Metal bindingi151Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi153Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi158Calcium 2By similarity1
Metal bindingi177Calcium 3By similarity1
Metal bindingi179Calcium 3By similarity1
Metal bindingi181Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi183Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi188Calcium 3By similarity1
Metal bindingi220Calcium 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi103 – 1141PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi147 – 1582PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi177 – 1883PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B24 908
ReactomeiR-BTA-1474228 Degradation of the extracellular matrix

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain small subunit 1
Short name:
CSS1
Alternative name(s):
Calcium-activated neutral proteinase small subunit
Short name:
CANP small subunit
Calcium-dependent protease small subunit
Short name:
CDPS
Calcium-dependent protease small subunit 1
Calpain regulatory subunit
Gene namesi
Name:CAPNS1
Synonyms:CAPN4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Organism-specific databases

VGNCiVGNC:52184 CAPNS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000737121 – 263Calpain small subunit 1Add BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei174N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP13135
PeptideAtlasiP13135
PRIDEiP13135

Expressioni

Gene expression databases

BgeeiENSBTAG00000014872

Interactioni

Subunit structurei

Homodimer or heterodimer of a large (catalytic) and a small (regulatory) subunit. In presence of calcium, the heterodimer dissociates (By similarity).By similarity

Protein-protein interaction databases

CORUMiP13135
STRINGi9913.ENSBTAP00000019803

Structurei

3D structure databases

ProteinModelPortaliP13135
SMRiP13135
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 125EF-hand 1; atypicalPROSITE-ProRule annotationAdd BLAST35
Domaini134 – 167EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini164 – 199EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini200 – 228EF-hand 4PROSITE-ProRule annotationAdd BLAST29
Domaini229 – 263EF-hand 5PROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 64Gly-rich (hydrophobic)Add BLAST64
Compositional biasi10 – 25Poly-GlyAdd BLAST16
Compositional biasi34 – 51Poly-GlyAdd BLAST18

Domaini

The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.By similarity
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037 Eukaryota
ENOG410YKQK LUCA
GeneTreeiENSGT00620000087734
HOGENOMiHOG000063658
HOVERGENiHBG004492
InParanoidiP13135
KOiK08583
OMAiSDEGGNM
OrthoDBiEOG091G0ISY
TreeFamiTF314682

Family and domain databases

InterProiView protein in InterPro
IPR029642 CAPN4
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PANTHERiPTHR44408:SF3 PTHR44408:SF3, 1 hit
PfamiView protein in Pfam
PF13833 EF-hand_8, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits

Sequencei

Sequence statusi: Complete.

P13135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLVNSFLKG GGGGGGGGGL GGGLGNVLGG LISGAGGGGG GGGGGGGGGG
60 70 80 90 100
GTAMRILGGV ISAISEAAAQ YNPEPVPPRT HYSNIEANES EEVRQFRRLF
110 120 130 140 150
AQLAGDDMEV SATELMNILN KVVTRHPDLK TDGFGIDTCR SMVAVMDSDT
160 170 180 190 200
TGKLGFEEFK YLWNNIKKWQ AVYKQFDVDR SGTIGSSELP GAFEAAGFRL
210 220 230 240 250
NEHLYNMIIR RYSDEGGNMD FDNFISCLVR LDAMFRAFKS LDKDGTGQIQ
260
VNIQEWLQLT MYS
Length:263
Mass (Da):27,931
Last modified:January 1, 1990 - v1
Checksum:i9427925D5284CE1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05065 mRNA Translation: AAA30422.1
EF139087 Genomic DNA Translation: ABL75413.1
BC116060 mRNA Translation: AAI16061.1
PIRiA34466
RefSeqiNP_776686.1, NM_174261.1
UniGeneiBt.7181

Genome annotation databases

EnsembliENSBTAT00000019803; ENSBTAP00000019803; ENSBTAG00000014872
GeneIDi281664
KEGGibta:281664

Similar proteinsi

Entry informationi

Entry nameiCPNS1_BOVIN
AccessioniPrimary (citable) accession number: P13135
Secondary accession number(s): A1YVU9, Q1JQC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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