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Protein

Calpain small subunit 1

Gene

CAPNS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi104Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi107Calcium 1By similarity1
Metal bindingi109Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi114Calcium 1By similarity1
Metal bindingi132Calcium 4By similarity1
Metal bindingi147Calcium 2By similarity1
Metal bindingi149Calcium 2By similarity1
Metal bindingi151Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi153Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi158Calcium 2By similarity1
Metal bindingi177Calcium 3By similarity1
Metal bindingi179Calcium 3By similarity1
Metal bindingi181Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi183Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi188Calcium 3By similarity1
Metal bindingi220Calcium 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi103 – 1141PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi147 – 1582PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi177 – 1883PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B24. 908.
ReactomeiR-BTA-1474228. Degradation of the extracellular matrix.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain small subunit 1
Short name:
CSS1
Alternative name(s):
Calcium-activated neutral proteinase small subunit
Short name:
CANP small subunit
Calcium-dependent protease small subunit
Short name:
CDPS
Calcium-dependent protease small subunit 1
Calpain regulatory subunit
Gene namesi
Name:CAPNS1
Synonyms:CAPN4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon calcium binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000737121 – 263Calpain small subunit 1Add BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei174N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP13135.
PeptideAtlasiP13135.
PRIDEiP13135.

Expressioni

Gene expression databases

BgeeiENSBTAG00000014872.

Interactioni

Subunit structurei

Homodimer or heterodimer of a large (catalytic) and a small (regulatory) subunit. In presence of calcium, the heterodimer dissociates (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019803.

Structurei

3D structure databases

ProteinModelPortaliP13135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 125EF-hand 1; atypicalPROSITE-ProRule annotationAdd BLAST35
Domaini134 – 167EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini164 – 199EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini200 – 228EF-hand 4PROSITE-ProRule annotationAdd BLAST29
Domaini229 – 263EF-hand 5PROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 64Gly-rich (hydrophobic)Add BLAST64
Compositional biasi10 – 25Poly-GlyAdd BLAST16
Compositional biasi34 – 51Poly-GlyAdd BLAST18

Domaini

The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.By similarity
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.

Sequence similaritiesi

Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000063658.
HOVERGENiHBG004492.
InParanoidiP13135.
KOiK08583.
OMAiGCLGGPH.
OrthoDBiEOG091G0ISY.
TreeFamiTF314682.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR029642. CAPN4.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF38. PTHR10183:SF38. 1 hit.
PfamiPF13833. EF-hand_8. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLVNSFLKG GGGGGGGGGL GGGLGNVLGG LISGAGGGGG GGGGGGGGGG
60 70 80 90 100
GTAMRILGGV ISAISEAAAQ YNPEPVPPRT HYSNIEANES EEVRQFRRLF
110 120 130 140 150
AQLAGDDMEV SATELMNILN KVVTRHPDLK TDGFGIDTCR SMVAVMDSDT
160 170 180 190 200
TGKLGFEEFK YLWNNIKKWQ AVYKQFDVDR SGTIGSSELP GAFEAAGFRL
210 220 230 240 250
NEHLYNMIIR RYSDEGGNMD FDNFISCLVR LDAMFRAFKS LDKDGTGQIQ
260
VNIQEWLQLT MYS
Length:263
Mass (Da):27,931
Last modified:January 1, 1990 - v1
Checksum:i9427925D5284CE1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05065 mRNA. Translation: AAA30422.1.
EF139087 Genomic DNA. Translation: ABL75413.1.
BC116060 mRNA. Translation: AAI16061.1.
PIRiA34466.
RefSeqiNP_776686.1. NM_174261.1.
UniGeneiBt.7181.

Genome annotation databases

EnsembliENSBTAT00000019803; ENSBTAP00000019803; ENSBTAG00000014872.
GeneIDi281664.
KEGGibta:281664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05065 mRNA. Translation: AAA30422.1.
EF139087 Genomic DNA. Translation: ABL75413.1.
BC116060 mRNA. Translation: AAI16061.1.
PIRiA34466.
RefSeqiNP_776686.1. NM_174261.1.
UniGeneiBt.7181.

3D structure databases

ProteinModelPortaliP13135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019803.

Proteomic databases

PaxDbiP13135.
PeptideAtlasiP13135.
PRIDEiP13135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000019803; ENSBTAP00000019803; ENSBTAG00000014872.
GeneIDi281664.
KEGGibta:281664.

Organism-specific databases

CTDi826.

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000063658.
HOVERGENiHBG004492.
InParanoidiP13135.
KOiK08583.
OMAiGCLGGPH.
OrthoDBiEOG091G0ISY.
TreeFamiTF314682.

Enzyme and pathway databases

BRENDAi3.4.22.B24. 908.
ReactomeiR-BTA-1474228. Degradation of the extracellular matrix.

Gene expression databases

BgeeiENSBTAG00000014872.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR029642. CAPN4.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF38. PTHR10183:SF38. 1 hit.
PfamiPF13833. EF-hand_8. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPNS1_BOVIN
AccessioniPrimary (citable) accession number: P13135
Secondary accession number(s): A1YVU9, Q1JQC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 5, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.