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P13123 (DN71_SULAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA-binding protein 7d
Alternative name(s):
7 kDa DNA-binding protein d
Sac7d

Cleaved into the following 2 chains:

  1. DNA-binding protein 7a
    Alternative name(s):
    7 kDa DNA-binding protein a
    Sac7a
  2. DNA-binding protein 7b
    Alternative name(s):
    7 kDa DNA-binding protein b
    Sac7b
Gene names
Ordered Locus Names:Saci_0064
OrganismSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]
Taxonomic identifier330779 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus ›

Protein attributes

Sequence length66 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature.

Subunit structure

Monomer.

Post-translational modification

Lys-5 was 70% monomethylated in form 7a, 25% in form 7b, and 20% in form 7d. Lys-7 was 50% monomethylated in form 7a, 40% in form 7b, and 50% in form 7d.

Sequence similarities

Belongs to the 7 kDa DNA-binding/endoribonuclease P2 family.

Ontologies

Keywords
   LigandDNA-binding
   PTMMethylation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

endoribonuclease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3 Ref.4
Chain2 – 6564DNA-binding protein 7d
PRO_0000007248
Chain2 – 6261DNA-binding protein 7a
PRO_0000007249
Chain2 – 5958DNA-binding protein 7b
PRO_0000007250

Amino acid modifications

Modified residue51N6-methyllysine; partial
Modified residue71N6-methyllysine; partial

Secondary structure

1 .............. 66
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13123 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: BFF84F058C2DDA97

FASTA667,609
        10         20         30         40         50         60 
MVKVKFKYKG EEKEVDTSKI KKVWRVGKMV SFTYDDNGKT GRGAVSEKDA PKELLDMLAR 


AEREKK

« Hide

References

« Hide 'large scale' references
[1]"Gene cloning, expression, and characterization of the Sac7 proteins from the hyperthermophile Sulfolobus acidocaldarius."
McAfee J.G., Edmondson S.P., Datta P.K., Shriver J.W., Gupta R.
Biochemistry 34:10063-10077(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[3]"The amino acid sequence of a small DNA binding protein from the archaebacterium Sulfolobus solfataricus."
Kimura M., Kimura J., Davie P., Reinhardt R., Dijk J.
FEBS Lett. 176:176-178(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-65.
[4]"Microsequence analysis of DNA-binding proteins 7a, 7b, and 7e from the archaebacterium Sulfolobus acidocaldarius."
Choli T., Wittmann-Liebold B., Reinhardt R.
J. Biol. Chem. 263:7087-7093(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-62.
[5]"Solution structure of the DNA-binding protein Sac7d from the hyperthermophile Sulfolobus acidocaldarius."
Edmondson S.P., Qiu L., Shriver J.W.
Biochemistry 34:13289-13304(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[6]"The hyperthermophile chromosomal protein Sac7d sharply kinks DNA."
Robinson H., Gao Y.-G., McCrary B.S., Edmondson S.P., Shriver J.W., Wang A.H.-J.
Nature 392:202-205(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M87569 Genomic DNA. Translation: AAA80315.1.
CP000077 Genomic DNA. Translation: AAY79492.1.
PIRA27749.
RefSeqYP_254785.1. NC_007181.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AZPX-ray1.60A1-66[»]
1AZQX-ray1.94A1-66[»]
1BF4X-ray1.60A4-58[»]
1CA5X-ray2.20A1-66[»]
1CA6X-ray2.20A1-66[»]
1SAPNMR-A1-66[»]
1WD0X-ray1.90A1-66[»]
1WD1X-ray2.20A1-66[»]
1WTOX-ray1.50A1-66[»]
1WTPX-ray1.90A/B1-66[»]
1WTQX-ray1.70A1-66[»]
1WTRX-ray1.80A1-66[»]
1WTVX-ray1.60A1-66[»]
1WTWX-ray2.20A1-66[»]
1WTXX-ray2.20A1-66[»]
1WVLX-ray2.60A/B1-66[»]
1XX8NMR-A1-66[»]
1XYIX-ray1.45A1-66[»]
2XIWX-ray1.50A/B2-66[»]
ProteinModelPortalP13123.
SMRP13123. Positions 1-66.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY79492; AAY79492; Saci_0064.
GeneID3473894.
KEGGsai:Saci_0064.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG86455.
HOGENOMHOG000270553.
KOK16640.
OMAMATKIKF.
ProtClustDBCLSK803180.

Enzyme and pathway databases

BioCycSACI330779:GH9J-149-MONOMER.

Family and domain databases

InterProIPR016197. Chromodomain-like.
IPR003212. DNA-bd_7kDa_arc.
[Graphical view]
PfamPF02294. 7kD_DNA_binding. 1 hit.
[Graphical view]
PIRSFPIRSF036912. Sac7. 1 hit.
ProDomPD010328. 7kDa_DNA_bd_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF54160. Chromodomain-like. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP13123.

Entry information

Entry nameDN71_SULAC
AccessionPrimary (citable) accession number: P13123
Secondary accession number(s): Q4JCI7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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