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P13123

- DN71_SULAC

UniProt

P13123 - DN71_SULAC

Protein

DNA-binding protein 7d

Gene

Saci_0064

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. endoribonuclease activity Source: InterPro

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciSACI330779:GH9J-64-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-binding protein 7d
    Alternative name(s):
    7 kDa DNA-binding protein d
    Sac7d
    Cleaved into the following 2 chains:
    Alternative name(s):
    7 kDa DNA-binding protein a
    Sac7a
    Alternative name(s):
    7 kDa DNA-binding protein b
    Sac7b
    Gene namesi
    Ordered Locus Names:Saci_0064
    OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
    Taxonomic identifieri330779 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001018: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 6564DNA-binding protein 7dPRO_0000007248Add
    BLAST
    Chaini2 – 6261DNA-binding protein 7aPRO_0000007249Add
    BLAST
    Chaini2 – 5958DNA-binding protein 7bPRO_0000007250Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei5 – 51N6-methyllysine; partial
    Modified residuei7 – 71N6-methyllysine; partial

    Post-translational modificationi

    Lys-5 was 70% monomethylated in form 7a, 25% in form 7b, and 20% in form 7d. Lys-7 was 50% monomethylated in form 7a, 40% in form 7b, and 50% in form 7d.

    Keywords - PTMi

    Methylation

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    66
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Beta strandi11 – 166
    Helixi17 – 193
    Beta strandi20 – 267
    Beta strandi29 – 368
    Beta strandi39 – 468
    Helixi47 – 493
    Helixi52 – 6211

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AZPX-ray1.60A1-66[»]
    1AZQX-ray1.94A1-66[»]
    1BF4X-ray1.60A4-66[»]
    1CA5X-ray2.20A1-66[»]
    1CA6X-ray2.20A1-66[»]
    1SAPNMR-A1-66[»]
    1WD0X-ray1.90A1-66[»]
    1WD1X-ray2.20A1-66[»]
    1WTOX-ray1.50A1-66[»]
    1WTPX-ray1.90A/B1-66[»]
    1WTQX-ray1.70A1-66[»]
    1WTRX-ray1.80A1-66[»]
    1WTVX-ray1.60A1-66[»]
    1WTWX-ray2.20A1-66[»]
    1WTXX-ray2.20A1-66[»]
    1WVLX-ray2.60A/B1-66[»]
    1XX8NMR-A1-66[»]
    1XYIX-ray1.45A1-66[»]
    2XIWX-ray1.50A/B2-66[»]
    4CJ1X-ray1.63B2-62[»]
    4CJ2X-ray1.50C/D2-66[»]
    ProteinModelPortaliP13123.
    SMRiP13123. Positions 1-66.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13123.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG86455.
    HOGENOMiHOG000270553.
    KOiK16640.
    OMAiMATKIKF.

    Family and domain databases

    InterProiIPR016197. Chromodomain-like.
    IPR003212. DNA-bd_7kDa_arc.
    [Graphical view]
    PfamiPF02294. 7kD_DNA_binding. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036912. Sac7. 1 hit.
    ProDomiPD010328. 7kDa_DNA_bd_arc. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF54160. SSF54160. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13123-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKVKFKYKG EEKEVDTSKI KKVWRVGKMV SFTYDDNGKT GRGAVSEKDA   50
    PKELLDMLAR AEREKK 66
    Length:66
    Mass (Da):7,609
    Last modified:January 23, 2007 - v3
    Checksum:iBFF84F058C2DDA97
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87569 Genomic DNA. Translation: AAA80315.1.
    CP000077 Genomic DNA. Translation: AAY79492.1.
    PIRiA27749.
    RefSeqiYP_254785.1. NC_007181.1.

    Genome annotation databases

    EnsemblBacteriaiAAY79492; AAY79492; Saci_0064.
    GeneIDi3473894.
    KEGGisai:Saci_0064.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87569 Genomic DNA. Translation: AAA80315.1 .
    CP000077 Genomic DNA. Translation: AAY79492.1 .
    PIRi A27749.
    RefSeqi YP_254785.1. NC_007181.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AZP X-ray 1.60 A 1-66 [» ]
    1AZQ X-ray 1.94 A 1-66 [» ]
    1BF4 X-ray 1.60 A 4-66 [» ]
    1CA5 X-ray 2.20 A 1-66 [» ]
    1CA6 X-ray 2.20 A 1-66 [» ]
    1SAP NMR - A 1-66 [» ]
    1WD0 X-ray 1.90 A 1-66 [» ]
    1WD1 X-ray 2.20 A 1-66 [» ]
    1WTO X-ray 1.50 A 1-66 [» ]
    1WTP X-ray 1.90 A/B 1-66 [» ]
    1WTQ X-ray 1.70 A 1-66 [» ]
    1WTR X-ray 1.80 A 1-66 [» ]
    1WTV X-ray 1.60 A 1-66 [» ]
    1WTW X-ray 2.20 A 1-66 [» ]
    1WTX X-ray 2.20 A 1-66 [» ]
    1WVL X-ray 2.60 A/B 1-66 [» ]
    1XX8 NMR - A 1-66 [» ]
    1XYI X-ray 1.45 A 1-66 [» ]
    2XIW X-ray 1.50 A/B 2-66 [» ]
    4CJ1 X-ray 1.63 B 2-62 [» ]
    4CJ2 X-ray 1.50 C/D 2-66 [» ]
    ProteinModelPortali P13123.
    SMRi P13123. Positions 1-66.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY79492 ; AAY79492 ; Saci_0064 .
    GeneIDi 3473894.
    KEGGi sai:Saci_0064.

    Phylogenomic databases

    eggNOGi NOG86455.
    HOGENOMi HOG000270553.
    KOi K16640.
    OMAi MATKIKF.

    Enzyme and pathway databases

    BioCyci SACI330779:GH9J-64-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P13123.

    Family and domain databases

    InterProi IPR016197. Chromodomain-like.
    IPR003212. DNA-bd_7kDa_arc.
    [Graphical view ]
    Pfami PF02294. 7kD_DNA_binding. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036912. Sac7. 1 hit.
    ProDomi PD010328. 7kDa_DNA_bd_arc. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF54160. SSF54160. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene cloning, expression, and characterization of the Sac7 proteins from the hyperthermophile Sulfolobus acidocaldarius."
      McAfee J.G., Edmondson S.P., Datta P.K., Shriver J.W., Gupta R.
      Biochemistry 34:10063-10077(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
      Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
      J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
    3. "The amino acid sequence of a small DNA binding protein from the archaebacterium Sulfolobus solfataricus."
      Kimura M., Kimura J., Davie P., Reinhardt R., Dijk J.
      FEBS Lett. 176:176-178(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-65.
    4. "Microsequence analysis of DNA-binding proteins 7a, 7b, and 7e from the archaebacterium Sulfolobus acidocaldarius."
      Choli T., Wittmann-Liebold B., Reinhardt R.
      J. Biol. Chem. 263:7087-7093(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-62.
    5. "Solution structure of the DNA-binding protein Sac7d from the hyperthermophile Sulfolobus acidocaldarius."
      Edmondson S.P., Qiu L., Shriver J.W.
      Biochemistry 34:13289-13304(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    6. "The hyperthermophile chromosomal protein Sac7d sharply kinks DNA."
      Robinson H., Gao Y.-G., McCrary B.S., Edmondson S.P., Shriver J.W., Wang A.H.-J.
      Nature 392:202-205(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiDN71_SULAC
    AccessioniPrimary (citable) accession number: P13123
    Secondary accession number(s): Q4JCI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 106 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3