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P13114 (CHSY_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase

EC=2.3.1.74
Alternative name(s):
Naringenin-chalcone synthase
Protein TRANSPARENT TESTA 4
Gene names
Name:CHS
Synonyms:TT4
Ordered Locus Names:At5g13930
ORF Names:MAC12.11, MAC12.14
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Sequence caution

The sequence AAM65314.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 395394Chalcone synthase
PRO_0000215954

Sites

Active site1691 By similarity

Amino acid modifications

Modified residue21N-acetylvaline

Experimental info

Mutagenesis3091H → Y in JK196.
Sequence conflict421P → H in AAL06937. Ref.7
Sequence conflict541M → K in AAL06937. Ref.7
Sequence conflict2681G → S in AAB35812. Ref.2
Sequence conflict3761L → S in AAL06937. Ref.7
Sequence conflict3841T → S in AAL06937. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P13114 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 3FCED2BCF2CCAB0E

FASTA39543,116
        10         20         30         40         50         60 
MVMAGASSLD EIRQAQRADG PAGILAIGTA NPENHVLQAE YPDYYFRITN SEHMTDLKEK 

        70         80         90        100        110        120 
FKRMCDKSTI RKRHMHLTEE FLKENPHMCA YMAPSLDTRQ DIVVVEVPKL GKEAAVKAIK 

       130        140        150        160        170        180 
EWGQPKSKIT HVVFCTTSGV DMPGADYQLT KLLGLRPSVK RLMMYQQGCF AGGTVLRIAK 

       190        200        210        220        230        240 
DLAENNRGAR VLVVCSEITA VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK 

       250        260        270        280        290        300 
PIFEMVSAAQ TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIVKSLD EAFKPLGISD 

       310        320        330        340        350        360 
WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVLSEY GNMSSACVLF ILDEMRRKSA 

       370        380        390 
KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL 

« Hide

References

« Hide 'large scale' references
[1]"Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene."
Feinbaum R.L., Ausubel F.M.
Mol. Cell. Biol. 8:1985-1992(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis."
Shirley B.W., Kubasek W.L., Storz G., Bruggemann E., Koornneef M., Ausubel F.M., Goodman H.M.
Plant J. 8:659-671(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[3]"Physiological and molecular characterization of transparent testa mutants of Arabidopsis impaired in phenylpropanoid pathway."
Bharti A.K., Tyagi A.K., Khurana J.P.
Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT JK196.
Strain: cv. Est.
[4]"Comparative evolutionary analysis of chalcone synthase and alcohol dehydrogenase loci in Arabidopsis, Arabis, and related genera (Brassicaceae)."
Koch M.A., Haubold B., Mitchell-Olds T.
Mol. Biol. Evol. 17:1483-1498(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[6]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[7]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[8]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana."
Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. expand/collapse author list , Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C., Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.
Plant J. 4:1051-1061(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 81-205 AND 348-395.
Strain: cv. Columbia.
Tissue: Green siliques.
[10]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT VAL-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[11]"The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
Plant Physiol. Biochem. 0:0-0(2013)
Cited for: REVIEW, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20308 Genomic DNA. Translation: AAA32771.1.
S80554 Genomic DNA. Translation: AAB35812.1.
Y18602 Genomic DNA. Translation: CAC80089.1.
Y18603 Genomic DNA. Translation: CAC80090.1.
AF112086 Genomic DNA. Translation: AAF23561.1.
AB005230 Genomic DNA. Translation: BAB11121.1.
CP002688 Genomic DNA. Translation: AED91961.1.
AY044331 mRNA. Translation: AAK73272.1.
AY054278 mRNA. Translation: AAL06937.1.
AY058155 mRNA. Translation: AAL25571.1.
AY090376 mRNA. Translation: AAL91279.1.
AY087778 mRNA. Translation: AAM65314.1. Different initiation.
BT000596 mRNA. Translation: AAN18165.1.
Z17650 mRNA. Translation: CAA79023.1.
Z17649 mRNA. Translation: CAA79022.1.
PIRSYMUCN. A27721.
RefSeqNP_196897.1. NM_121396.3.
UniGeneAt.297.
At.69414.
At.71936.

3D structure databases

ProteinModelPortalP13114.
SMRP13114. Positions 8-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP13114. 6 interactions.
MINTMINT-8184522.

Proteomic databases

PaxDbP13114.
PRIDEP13114.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G13930.1; AT5G13930.1; AT5G13930.
GeneID831241.
KEGGath:AT5G13930.

Organism-specific databases

TAIRAT5G13930.

Phylogenomic databases

eggNOGCOG3424.
HOGENOMHOG000245460.
InParanoidP13114.
KOK00660.
OMALHEVFMS.
PhylomeDBP13114.
ProtClustDBCLSN2916621.

Enzyme and pathway databases

BioCycARA:AT5G13930-MONOMER.
MetaCyc:AT5G13930-MONOMER.
BRENDA2.3.1.74. 399.
UniPathwayUPA00154.

Gene expression databases

ArrayExpressP13114.
GenevestigatorP13114.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHSY_ARATH
AccessionPrimary (citable) accession number: P13114
Secondary accession number(s): Q43132, Q940L6, Q941R9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: March 19, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names