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P13114

- CHSY_ARATH

UniProt

P13114 - CHSY_ARATH

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Protein

Chalcone synthase

Gene

CHS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei169 – 1691PROSITE-ProRule annotation

GO - Molecular functioni

  1. naringenin-chalcone synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. auxin polar transport Source: TAIR
  2. flavonoid biosynthetic process Source: TAIR
  3. regulation of anthocyanin biosynthetic process Source: TAIR
  4. response to auxin Source: TAIR
  5. response to gravity Source: TAIR
  6. response to jasmonic acid Source: TAIR
  7. response to oxidative stress Source: TAIR
  8. response to UV-B Source: TAIR
  9. response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

BioCyciARA:AT5G13930-MONOMER.
MetaCyc:AT5G13930-MONOMER.
BRENDAi2.3.1.74. 399.
UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase
Protein TRANSPARENT TESTA 4
Gene namesi
Name:CHS
Synonyms:TT4
Ordered Locus Names:At5g13930
ORF Names:MAC12.11, MAC12.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G13930.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. nucleus Source: TAIR
  3. plant-type vacuole membrane Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi309 – 3091H → Y in JK196.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 395394Chalcone synthasePRO_0000215954Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylvaline1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP13114.
PRIDEiP13114.

Expressioni

Gene expression databases

GenevestigatoriP13114.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CHI1P410884EBI-1546775,EBI-1546761
DFRAP511024EBI-1546775,EBI-1546795
FLS1Q963303EBI-1546775,EBI-1774454

Protein-protein interaction databases

BioGridi16519. 3 interactions.
IntActiP13114. 6 interactions.
MINTiMINT-8184522.

Structurei

3D structure databases

ProteinModelPortaliP13114.
SMRiP13114. Positions 8-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Phylogenomic databases

eggNOGiCOG3424.
HOGENOMiHOG000245460.
InParanoidiP13114.
KOiK00660.
OMAiTANPENH.
PhylomeDBiP13114.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13114-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVMAGASSLD EIRQAQRADG PAGILAIGTA NPENHVLQAE YPDYYFRITN
60 70 80 90 100
SEHMTDLKEK FKRMCDKSTI RKRHMHLTEE FLKENPHMCA YMAPSLDTRQ
110 120 130 140 150
DIVVVEVPKL GKEAAVKAIK EWGQPKSKIT HVVFCTTSGV DMPGADYQLT
160 170 180 190 200
KLLGLRPSVK RLMMYQQGCF AGGTVLRIAK DLAENNRGAR VLVVCSEITA
210 220 230 240 250
VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK PIFEMVSAAQ
260 270 280 290 300
TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIVKSLD EAFKPLGISD
310 320 330 340 350
WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVLSEY GNMSSACVLF
360 370 380 390
ILDEMRRKSA KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL
Length:395
Mass (Da):43,116
Last modified:January 1, 1990 - v1
Checksum:i3FCED2BCF2CCAB0E
GO

Sequence cautioni

The sequence AAM65314.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421P → H in AAL06937. (PubMed:14593172)Curated
Sequence conflicti54 – 541M → K in AAL06937. (PubMed:14593172)Curated
Sequence conflicti268 – 2681G → S in AAB35812. (PubMed:8528278)Curated
Sequence conflicti376 – 3761L → S in AAL06937. (PubMed:14593172)Curated
Sequence conflicti384 – 3841T → S in AAL06937. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20308 Genomic DNA. Translation: AAA32771.1.
S80554 Genomic DNA. Translation: AAB35812.1.
Y18602 Genomic DNA. Translation: CAC80089.1.
Y18603 Genomic DNA. Translation: CAC80090.1.
AF112086 Genomic DNA. Translation: AAF23561.1.
AB005230 Genomic DNA. Translation: BAB11121.1.
CP002688 Genomic DNA. Translation: AED91961.1.
AY044331 mRNA. Translation: AAK73272.1.
AY054278 mRNA. Translation: AAL06937.1.
AY058155 mRNA. Translation: AAL25571.1.
AY090376 mRNA. Translation: AAL91279.1.
AY087778 mRNA. Translation: AAM65314.1. Different initiation.
BT000596 mRNA. Translation: AAN18165.1.
Z17650 mRNA. Translation: CAA79023.1.
Z17649 mRNA. Translation: CAA79022.1.
PIRiA27721. SYMUCN.
RefSeqiNP_196897.1. NM_121396.3.
UniGeneiAt.297.
At.69414.
At.71936.

Genome annotation databases

EnsemblPlantsiAT5G13930.1; AT5G13930.1; AT5G13930.
GeneIDi831241.
KEGGiath:AT5G13930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20308 Genomic DNA. Translation: AAA32771.1 .
S80554 Genomic DNA. Translation: AAB35812.1 .
Y18602 Genomic DNA. Translation: CAC80089.1 .
Y18603 Genomic DNA. Translation: CAC80090.1 .
AF112086 Genomic DNA. Translation: AAF23561.1 .
AB005230 Genomic DNA. Translation: BAB11121.1 .
CP002688 Genomic DNA. Translation: AED91961.1 .
AY044331 mRNA. Translation: AAK73272.1 .
AY054278 mRNA. Translation: AAL06937.1 .
AY058155 mRNA. Translation: AAL25571.1 .
AY090376 mRNA. Translation: AAL91279.1 .
AY087778 mRNA. Translation: AAM65314.1 . Different initiation.
BT000596 mRNA. Translation: AAN18165.1 .
Z17650 mRNA. Translation: CAA79023.1 .
Z17649 mRNA. Translation: CAA79022.1 .
PIRi A27721. SYMUCN.
RefSeqi NP_196897.1. NM_121396.3.
UniGenei At.297.
At.69414.
At.71936.

3D structure databases

ProteinModelPortali P13114.
SMRi P13114. Positions 8-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 16519. 3 interactions.
IntActi P13114. 6 interactions.
MINTi MINT-8184522.

Proteomic databases

PaxDbi P13114.
PRIDEi P13114.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G13930.1 ; AT5G13930.1 ; AT5G13930 .
GeneIDi 831241.
KEGGi ath:AT5G13930.

Organism-specific databases

TAIRi AT5G13930.

Phylogenomic databases

eggNOGi COG3424.
HOGENOMi HOG000245460.
InParanoidi P13114.
KOi K00660.
OMAi TANPENH.
PhylomeDBi P13114.

Enzyme and pathway databases

UniPathwayi UPA00154 .
BioCyci ARA:AT5G13930-MONOMER.
MetaCyc:AT5G13930-MONOMER.
BRENDAi 2.3.1.74. 399.

Gene expression databases

Genevestigatori P13114.

Family and domain databases

Gene3Di 3.40.47.10. 2 hits.
InterProi IPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000451. PKS_III. 1 hit.
SUPFAMi SSF53901. SSF53901. 2 hits.
PROSITEi PS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene."
    Feinbaum R.L., Ausubel F.M.
    Mol. Cell. Biol. 8:1985-1992(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis."
    Shirley B.W., Kubasek W.L., Storz G., Bruggemann E., Koornneef M., Ausubel F.M., Goodman H.M.
    Plant J. 8:659-671(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  3. "Physiological and molecular characterization of transparent testa mutants of Arabidopsis impaired in phenylpropanoid pathway."
    Bharti A.K., Tyagi A.K., Khurana J.P.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT JK196.
    Strain: cv. Est.
  4. "Comparative evolutionary analysis of chalcone synthase and alcohol dehydrogenase loci in Arabidopsis, Arabis, and related genera (Brassicaceae)."
    Koch M.A., Haubold B., Mitchell-Olds T.
    Mol. Biol. Evol. 17:1483-1498(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  6. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 81-205 AND 348-395.
    Strain: cv. Columbia.
    Tissue: Green siliques.
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT VAL-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
    Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
    Plant Physiol. Biochem. 0:0-0(2013)
    Cited for: REVIEW, NOMENCLATURE.

Entry informationi

Entry nameiCHSY_ARATH
AccessioniPrimary (citable) accession number: P13114
Secondary accession number(s): Q43132, Q940L6, Q941R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3