Chalcone synthase - Arabidopsis thaliana (Mouse-ear cress)

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P13114 (CHSY_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chalcone synthase
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase
Protein TRANSPARENT TESTA 4

Gene names

Name:	CHS
Synonyms:	TT4
Ordered Locus Names:	At5g13930
ORF Names:	MAC12.11, MAC12.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO₂.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.
Sequence caution	The sequence AAM65314.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	auxin polar transport Inferred from mutant phenotype PubMed 11402184. Source: TAIR flavonoid biosynthetic process Inferred from mutant phenotype PubMed 21427279. Source: TAIR regulation of anthocyanin biosynthetic process Inferred from expression pattern PubMed 15993620. Source: TAIR response to UV-B Inferred from mutant phenotype PubMed 12271060. Source: TAIR response to auxin stimulus Inferred from expression pattern PubMed 21427279. Source: TAIR response to gravity Inferred from mutant phenotype PubMed 16489132. Source: TAIR response to jasmonic acid stimulus Inferred from expression pattern PubMed 16021335. Source: TAIR response to oxidative stress Inferred from expression pattern PubMed 9501110. Source: TAIR response to wounding Inferred from expression pattern PubMed 16021335. Source: TAIR
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 11489181 PubMed 15817473. Source: TAIR nucleus Inferred from direct assay PubMed 15817473. Source: TAIR plant-type vacuole membrane Inferred from direct assay PubMed 11489181. Source: TAIR
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CHI1	P41088	4	EBI-1546775,EBI-1546761

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 395

395

Chalcone synthase

PRO_0000215954

Sites

Active site

169

By similarity

Experimental info

Mutagenesis

309

H → Y in JK196.

Sequence conflict

P → H in AAL06937. Ref.7

Sequence conflict

M → K in AAL06937. Ref.7

Sequence conflict

268

G → S in AAB35812. Ref.2

Sequence conflict

376

L → S in AAL06937. Ref.7

Sequence conflict

384

T → S in AAL06937. Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P13114 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 3FCED2BCF2CCAB0E
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FASTA

395

43,116

        10         20         30         40         50         60 
MVMAGASSLD EIRQAQRADG PAGILAIGTA NPENHVLQAE YPDYYFRITN SEHMTDLKEK 

        70         80         90        100        110        120 
FKRMCDKSTI RKRHMHLTEE FLKENPHMCA YMAPSLDTRQ DIVVVEVPKL GKEAAVKAIK 

       130        140        150        160        170        180 
EWGQPKSKIT HVVFCTTSGV DMPGADYQLT KLLGLRPSVK RLMMYQQGCF AGGTVLRIAK 

       190        200        210        220        230        240 
DLAENNRGAR VLVVCSEITA VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK 

       250        260        270        280        290        300 
PIFEMVSAAQ TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIVKSLD EAFKPLGISD 

       310        320        330        340        350        360 
WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVLSEY GNMSSACVLF ILDEMRRKSA 

       370        380        390 
KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene." Feinbaum R.L., Ausubel F.M. Mol. Cell. Biol. 8:1985-1992(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis." Shirley B.W., Kubasek W.L., Storz G., Bruggemann E., Koornneef M., Ausubel F.M., Goodman H.M. Plant J. 8:659-671(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[3]	"Physiological and molecular characterization of transparent testa mutants of Arabidopsis impaired in phenylpropanoid pathway." Bharti A.K., Tyagi A.K., Khurana J.P. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT JK196. Strain: cv. Est.
[4]	"Comparative evolutionary analysis of chalcone synthase and alcohol dehydrogenase loci in Arabidopsis, Arabis, and related genera (Brassicaceae)." Koch M.A., Haubold B., Mitchell-Olds T. Mol. Biol. Evol. 17:1483-1498(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[6]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[7]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[8]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]	"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana." Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. Lescure B. Plant J. 4:1051-1061(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 81-205 AND 348-395. Strain: cv. Columbia. Tissue: Green siliques.
[10]	"The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity." Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R. Plant Physiol. Biochem. 0:0-0(2013) Cited for: REVIEW, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20308 Genomic DNA. Translation: AAA32771.1. S80554 Genomic DNA. Translation: AAB35812.1. Y18602 Genomic DNA. Translation: CAC80089.1. Y18603 Genomic DNA. Translation: CAC80090.1. AF112086 Genomic DNA. Translation: AAF23561.1. AB005230 Genomic DNA. Translation: BAB11121.1. CP002688 Genomic DNA. Translation: AED91961.1. AY044331 mRNA. Translation: AAK73272.1. AY054278 mRNA. Translation: AAL06937.1. AY058155 mRNA. Translation: AAL25571.1. AY090376 mRNA. Translation: AAL91279.1. AY087778 mRNA. Translation: AAM65314.1. Different initiation. BT000596 mRNA. Translation: AAN18165.1. Z17650 mRNA. Translation: CAA79023.1. Z17649 mRNA. Translation: CAA79022.1.
IPI	IPI00545547.
PIR	SYMUCN. A27721.
RefSeq	NP_196897.1. NM_121396.3.
UniGene	At.297. At.69414. At.71936.
3D structure databases
ProteinModelPortal	P13114.
SMR	P13114. Positions 8-395.
ModBase	Search...
Protein-protein interaction databases
IntAct	P13114. 6 interactions.
MINT	MINT-8184522.
Proteomic databases
PaxDb	P13114.
PRIDE	P13114.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G13930.1; AT5G13930.1; AT5G13930.
GeneID	831241.
KEGG	ath:AT5G13930.
Organism-specific databases
TAIR	At5g13930.
Phylogenomic databases
eggNOG	COG3424.
HOGENOM	HOG000245460.
InParanoid	P13114.
KO	K00660.
OMA	FIAHPGG.
PhylomeDB	P13114.
ProtClustDB	CLSN2916621.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G13930-MONOMER.
BRENDA	2.3.1.74. 399.
UniPathway	UPA00154.
Gene expression databases
ArrayExpress	P13114.
Genevestigator	P13114.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHSY_ARATH

Accession

Primary (citable) accession number: P13114
Secondary accession number(s): Q43132, Q940L6, Q941R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 29, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents