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P13114

- CHSY_ARATH

UniProt

P13114 - CHSY_ARATH

Protein

Chalcone synthase

Gene

CHS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

    Catalytic activityi

    3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei169 – 1691PROSITE-ProRule annotation

    GO - Molecular functioni

    1. naringenin-chalcone synthase activity Source: UniProtKB-EC
    2. protein binding Source: IntAct

    GO - Biological processi

    1. auxin polar transport Source: TAIR
    2. flavonoid biosynthetic process Source: TAIR
    3. regulation of anthocyanin biosynthetic process Source: TAIR
    4. response to auxin Source: TAIR
    5. response to gravity Source: TAIR
    6. response to jasmonic acid Source: TAIR
    7. response to oxidative stress Source: TAIR
    8. response to UV-B Source: TAIR
    9. response to wounding Source: TAIR

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT5G13930-MONOMER.
    MetaCyc:AT5G13930-MONOMER.
    BRENDAi2.3.1.74. 399.
    UniPathwayiUPA00154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chalcone synthase (EC:2.3.1.74)
    Alternative name(s):
    Naringenin-chalcone synthase
    Protein TRANSPARENT TESTA 4
    Gene namesi
    Name:CHS
    Synonyms:TT4
    Ordered Locus Names:At5g13930
    ORF Names:MAC12.11, MAC12.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G13930.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. nucleus Source: TAIR
    3. plant-type vacuole membrane Source: TAIR

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi309 – 3091H → Y in JK196.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 395394Chalcone synthasePRO_0000215954Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylvaline1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP13114.
    PRIDEiP13114.

    Expressioni

    Gene expression databases

    GenevestigatoriP13114.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CHI1P410884EBI-1546775,EBI-1546761
    DFRAP511024EBI-1546775,EBI-1546795
    FLS1Q963303EBI-1546775,EBI-1774454

    Protein-protein interaction databases

    BioGridi16519. 3 interactions.
    IntActiP13114. 6 interactions.
    MINTiMINT-8184522.

    Structurei

    3D structure databases

    ProteinModelPortaliP13114.
    SMRiP13114. Positions 8-395.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.Curated

    Phylogenomic databases

    eggNOGiCOG3424.
    HOGENOMiHOG000245460.
    InParanoidiP13114.
    KOiK00660.
    OMAiTANPENH.
    PhylomeDBiP13114.

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13114-1 [UniParc]FASTAAdd to Basket

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    MVMAGASSLD EIRQAQRADG PAGILAIGTA NPENHVLQAE YPDYYFRITN    50
    SEHMTDLKEK FKRMCDKSTI RKRHMHLTEE FLKENPHMCA YMAPSLDTRQ 100
    DIVVVEVPKL GKEAAVKAIK EWGQPKSKIT HVVFCTTSGV DMPGADYQLT 150
    KLLGLRPSVK RLMMYQQGCF AGGTVLRIAK DLAENNRGAR VLVVCSEITA 200
    VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK PIFEMVSAAQ 250
    TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIVKSLD EAFKPLGISD 300
    WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVLSEY GNMSSACVLF 350
    ILDEMRRKSA KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL 395
    Length:395
    Mass (Da):43,116
    Last modified:January 1, 1990 - v1
    Checksum:i3FCED2BCF2CCAB0E
    GO

    Sequence cautioni

    The sequence AAM65314.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti42 – 421P → H in AAL06937. (PubMed:14593172)Curated
    Sequence conflicti54 – 541M → K in AAL06937. (PubMed:14593172)Curated
    Sequence conflicti268 – 2681G → S in AAB35812. (PubMed:8528278)Curated
    Sequence conflicti376 – 3761L → S in AAL06937. (PubMed:14593172)Curated
    Sequence conflicti384 – 3841T → S in AAL06937. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20308 Genomic DNA. Translation: AAA32771.1.
    S80554 Genomic DNA. Translation: AAB35812.1.
    Y18602 Genomic DNA. Translation: CAC80089.1.
    Y18603 Genomic DNA. Translation: CAC80090.1.
    AF112086 Genomic DNA. Translation: AAF23561.1.
    AB005230 Genomic DNA. Translation: BAB11121.1.
    CP002688 Genomic DNA. Translation: AED91961.1.
    AY044331 mRNA. Translation: AAK73272.1.
    AY054278 mRNA. Translation: AAL06937.1.
    AY058155 mRNA. Translation: AAL25571.1.
    AY090376 mRNA. Translation: AAL91279.1.
    AY087778 mRNA. Translation: AAM65314.1. Different initiation.
    BT000596 mRNA. Translation: AAN18165.1.
    Z17650 mRNA. Translation: CAA79023.1.
    Z17649 mRNA. Translation: CAA79022.1.
    PIRiA27721. SYMUCN.
    RefSeqiNP_196897.1. NM_121396.3.
    UniGeneiAt.297.
    At.69414.
    At.71936.

    Genome annotation databases

    EnsemblPlantsiAT5G13930.1; AT5G13930.1; AT5G13930.
    GeneIDi831241.
    KEGGiath:AT5G13930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20308 Genomic DNA. Translation: AAA32771.1 .
    S80554 Genomic DNA. Translation: AAB35812.1 .
    Y18602 Genomic DNA. Translation: CAC80089.1 .
    Y18603 Genomic DNA. Translation: CAC80090.1 .
    AF112086 Genomic DNA. Translation: AAF23561.1 .
    AB005230 Genomic DNA. Translation: BAB11121.1 .
    CP002688 Genomic DNA. Translation: AED91961.1 .
    AY044331 mRNA. Translation: AAK73272.1 .
    AY054278 mRNA. Translation: AAL06937.1 .
    AY058155 mRNA. Translation: AAL25571.1 .
    AY090376 mRNA. Translation: AAL91279.1 .
    AY087778 mRNA. Translation: AAM65314.1 . Different initiation.
    BT000596 mRNA. Translation: AAN18165.1 .
    Z17650 mRNA. Translation: CAA79023.1 .
    Z17649 mRNA. Translation: CAA79022.1 .
    PIRi A27721. SYMUCN.
    RefSeqi NP_196897.1. NM_121396.3.
    UniGenei At.297.
    At.69414.
    At.71936.

    3D structure databases

    ProteinModelPortali P13114.
    SMRi P13114. Positions 8-395.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 16519. 3 interactions.
    IntActi P13114. 6 interactions.
    MINTi MINT-8184522.

    Proteomic databases

    PaxDbi P13114.
    PRIDEi P13114.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G13930.1 ; AT5G13930.1 ; AT5G13930 .
    GeneIDi 831241.
    KEGGi ath:AT5G13930.

    Organism-specific databases

    TAIRi AT5G13930.

    Phylogenomic databases

    eggNOGi COG3424.
    HOGENOMi HOG000245460.
    InParanoidi P13114.
    KOi K00660.
    OMAi TANPENH.
    PhylomeDBi P13114.

    Enzyme and pathway databases

    UniPathwayi UPA00154 .
    BioCyci ARA:AT5G13930-MONOMER.
    MetaCyc:AT5G13930-MONOMER.
    BRENDAi 2.3.1.74. 399.

    Gene expression databases

    Genevestigatori P13114.

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000451. PKS_III. 1 hit.
    SUPFAMi SSF53901. SSF53901. 2 hits.
    PROSITEi PS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene."
      Feinbaum R.L., Ausubel F.M.
      Mol. Cell. Biol. 8:1985-1992(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis."
      Shirley B.W., Kubasek W.L., Storz G., Bruggemann E., Koornneef M., Ausubel F.M., Goodman H.M.
      Plant J. 8:659-671(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Landsberg erecta.
    3. "Physiological and molecular characterization of transparent testa mutants of Arabidopsis impaired in phenylpropanoid pathway."
      Bharti A.K., Tyagi A.K., Khurana J.P.
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT JK196.
      Strain: cv. Est.
    4. "Comparative evolutionary analysis of chalcone synthase and alcohol dehydrogenase loci in Arabidopsis, Arabis, and related genera (Brassicaceae)."
      Koch M.A., Haubold B., Mitchell-Olds T.
      Mol. Biol. Evol. 17:1483-1498(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    6. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    8. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 81-205 AND 348-395.
      Strain: cv. Columbia.
      Tissue: Green siliques.
    10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT VAL-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    11. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
      Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
      Plant Physiol. Biochem. 0:0-0(2013)
      Cited for: REVIEW, NOMENCLATURE.

    Entry informationi

    Entry nameiCHSY_ARATH
    AccessioniPrimary (citable) accession number: P13114
    Secondary accession number(s): Q43132, Q940L6, Q941R9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3