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Protein

Chalcone synthase

Gene

CHS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei169PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • auxin polar transport Source: TAIR
  • flavonoid biosynthetic process Source: TAIR
  • regulation of anthocyanin biosynthetic process Source: TAIR
  • response to auxin Source: TAIR
  • response to gravity Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to UV-B Source: TAIR
  • response to wounding Source: TAIR

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFlavonoid biosynthesis

Enzyme and pathway databases

BioCyciARA:AT5G13930-MONOMER
MetaCyc:AT5G13930-MONOMER
BRENDAi2.3.1.74 399
UniPathwayiUPA00154

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase
Protein TRANSPARENT TESTA 4
Gene namesi
Name:CHS
Synonyms:TT4
Ordered Locus Names:At5g13930
ORF Names:MAC12.11, MAC12.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G13930
TAIRilocus:2159098 AT5G13930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi309H → Y in JK196. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002159542 – 395Chalcone synthaseAdd BLAST394
Isoform 2 (identifier: P13114-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylvalineCombined sources1
Isoform 2 (identifier: P13114-2)
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP13114
PRIDEiP13114

PTM databases

iPTMnetiP13114

Expressioni

Gene expression databases

ExpressionAtlasiP13114 baseline and differential
GenevisibleiP13114 AT

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi16519, 8 interactors
IntActiP13114, 6 interactors
MINTiP13114
STRINGi3702.AT5G13930.1

Structurei

3D structure databases

ProteinModelPortaliP13114
SMRiP13114
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Phylogenomic databases

eggNOGiENOG410IKG2 Eukaryota
COG3424 LUCA
HOGENOMiHOG000245460
InParanoidiP13114
KOiK00660
OMAiPIWGLGC
OrthoDBiEOG09360E8Q
PhylomeDBiP13114

Family and domain databases

Gene3Di3.40.47.10, 2 hits
InterProiView protein in InterPro
IPR012328 Chalcone/stilbene_synth_C
IPR018088 Chalcone/stilbene_synthase_AS
IPR001099 Chalcone/stilbene_synthase_N
IPR011141 Polyketide_synthase_type-III
IPR016039 Thiolase-like
PANTHERiPTHR11877 PTHR11877, 1 hit
PfamiView protein in Pfam
PF02797 Chal_sti_synt_C, 1 hit
PF00195 Chal_sti_synt_N, 1 hit
PIRSFiPIRSF000451 PKS_III, 1 hit
SUPFAMiSSF53901 SSF53901, 2 hits
PROSITEiView protein in PROSITE
PS00441 CHALCONE_SYNTH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P13114-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMAGASSLD EIRQAQRADG PAGILAIGTA NPENHVLQAE YPDYYFRITN
60 70 80 90 100
SEHMTDLKEK FKRMCDKSTI RKRHMHLTEE FLKENPHMCA YMAPSLDTRQ
110 120 130 140 150
DIVVVEVPKL GKEAAVKAIK EWGQPKSKIT HVVFCTTSGV DMPGADYQLT
160 170 180 190 200
KLLGLRPSVK RLMMYQQGCF AGGTVLRIAK DLAENNRGAR VLVVCSEITA
210 220 230 240 250
VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK PIFEMVSAAQ
260 270 280 290 300
TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIVKSLD EAFKPLGISD
310 320 330 340 350
WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVLSEY GNMSSACVLF
360 370 380 390
ILDEMRRKSA KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL
Length:395
Mass (Da):43,116
Last modified:January 1, 1990 - v1
Checksum:i3FCED2BCF2CCAB0E
GO
Isoform 2 (identifier: P13114-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Show »
Length:393
Mass (Da):42,885
Checksum:i771638EAE98566E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42P → H in AAL06937 (PubMed:14593172).Curated1
Sequence conflicti54M → K in AAL06937 (PubMed:14593172).Curated1
Sequence conflicti268G → S in AAB35812 (PubMed:8528278).Curated1
Sequence conflicti376L → S in AAL06937 (PubMed:14593172).Curated1
Sequence conflicti384T → S in AAL06937 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0579451 – 2Missing in isoform 2. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20308 Genomic DNA Translation: AAA32771.1
S80554 Genomic DNA Translation: AAB35812.1
Y18602 Genomic DNA Translation: CAC80089.1
Y18603 Genomic DNA Translation: CAC80090.1
AF112086 Genomic DNA Translation: AAF23561.1
AB005230 Genomic DNA Translation: BAB11121.1
CP002688 Genomic DNA Translation: AED91961.1
AY044331 mRNA Translation: AAK73272.1
AY054278 mRNA Translation: AAL06937.1
AY058155 mRNA Translation: AAL25571.1
AY090376 mRNA Translation: AAL91279.1
AY087778 mRNA Translation: AAM65314.1
BT000596 mRNA Translation: AAN18165.1
Z17650 mRNA Translation: CAA79023.1
Z17649 mRNA Translation: CAA79022.1
PIRiA27721 SYMUCN
RefSeqiNP_196897.1, NM_121396.4 [P13114-1]
UniGeneiAt.297
At.69414
At.71936

Genome annotation databases

EnsemblPlantsiAT5G13930.1; AT5G13930.1; AT5G13930 [P13114-1]
GeneIDi831241
GrameneiAT5G13930.1; AT5G13930.1; AT5G13930 [P13114-1]
KEGGiath:AT5G13930

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiCHSY_ARATH
AccessioniPrimary (citable) accession number: P13114
Secondary accession number(s): Q43132, Q940L6, Q941R9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 25, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health