Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chalcone synthase

Gene

CHS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei169PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • auxin polar transport Source: TAIR
  • flavonoid biosynthetic process Source: TAIR
  • regulation of anthocyanin biosynthetic process Source: TAIR
  • response to auxin Source: TAIR
  • response to gravity Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to UV-B Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:AT5G13930-MONOMER.
BRENDAi2.3.1.74. 399.
UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase
Protein TRANSPARENT TESTA 4
Gene namesi
Name:CHS
Synonyms:TT4
Ordered Locus Names:At5g13930
ORF Names:MAC12.11, MAC12.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G13930.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • nucleus Source: TAIR
  • plant-type vacuole membrane Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi309H → Y in JK196. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002159542 – 395Chalcone synthaseAdd BLAST394
Isoform 2 (identifier: P13114-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylvalineCombined sources1
Isoform 2 (identifier: P13114-2)
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP13114.
PRIDEiP13114.

PTM databases

iPTMnetiP13114.

Expressioni

Gene expression databases

ExpressionAtlasiP13114. baseline and differential.
GenevisibleiP13114. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CHI1P410884EBI-1546775,EBI-1546761
DFRAP511024EBI-1546775,EBI-1546795
FLS1Q963303EBI-1546775,EBI-1774454

Protein-protein interaction databases

BioGridi16519. 8 interactors.
IntActiP13114. 6 interactors.
MINTiMINT-8184522.
STRINGi3702.AT5G13930.1.

Structurei

3D structure databases

ProteinModelPortaliP13114.
SMRiP13114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Phylogenomic databases

eggNOGiENOG410IKG2. Eukaryota.
COG3424. LUCA.
HOGENOMiHOG000245460.
InParanoidiP13114.
KOiK00660.
OMAiNICEYMA.
OrthoDBiEOG09360E8Q.
PhylomeDBiP13114.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P13114-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMAGASSLD EIRQAQRADG PAGILAIGTA NPENHVLQAE YPDYYFRITN
60 70 80 90 100
SEHMTDLKEK FKRMCDKSTI RKRHMHLTEE FLKENPHMCA YMAPSLDTRQ
110 120 130 140 150
DIVVVEVPKL GKEAAVKAIK EWGQPKSKIT HVVFCTTSGV DMPGADYQLT
160 170 180 190 200
KLLGLRPSVK RLMMYQQGCF AGGTVLRIAK DLAENNRGAR VLVVCSEITA
210 220 230 240 250
VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK PIFEMVSAAQ
260 270 280 290 300
TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIVKSLD EAFKPLGISD
310 320 330 340 350
WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVLSEY GNMSSACVLF
360 370 380 390
ILDEMRRKSA KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL
Length:395
Mass (Da):43,116
Last modified:January 1, 1990 - v1
Checksum:i3FCED2BCF2CCAB0E
GO
Isoform 2 (identifier: P13114-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Show »
Length:393
Mass (Da):42,885
Checksum:i771638EAE98566E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42P → H in AAL06937 (PubMed:14593172).Curated1
Sequence conflicti54M → K in AAL06937 (PubMed:14593172).Curated1
Sequence conflicti268G → S in AAB35812 (PubMed:8528278).Curated1
Sequence conflicti376L → S in AAL06937 (PubMed:14593172).Curated1
Sequence conflicti384T → S in AAL06937 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0579451 – 2Missing in isoform 2. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20308 Genomic DNA. Translation: AAA32771.1.
S80554 Genomic DNA. Translation: AAB35812.1.
Y18602 Genomic DNA. Translation: CAC80089.1.
Y18603 Genomic DNA. Translation: CAC80090.1.
AF112086 Genomic DNA. Translation: AAF23561.1.
AB005230 Genomic DNA. Translation: BAB11121.1.
CP002688 Genomic DNA. Translation: AED91961.1.
AY044331 mRNA. Translation: AAK73272.1.
AY054278 mRNA. Translation: AAL06937.1.
AY058155 mRNA. Translation: AAL25571.1.
AY090376 mRNA. Translation: AAL91279.1.
AY087778 mRNA. Translation: AAM65314.1.
BT000596 mRNA. Translation: AAN18165.1.
Z17650 mRNA. Translation: CAA79023.1.
Z17649 mRNA. Translation: CAA79022.1.
PIRiA27721. SYMUCN.
RefSeqiNP_196897.1. NM_121396.4. [P13114-1]
UniGeneiAt.297.
At.69414.
At.71936.

Genome annotation databases

EnsemblPlantsiAT5G13930.1; AT5G13930.1; AT5G13930. [P13114-1]
GeneIDi831241.
GrameneiAT5G13930.1; AT5G13930.1; AT5G13930.
KEGGiath:AT5G13930.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20308 Genomic DNA. Translation: AAA32771.1.
S80554 Genomic DNA. Translation: AAB35812.1.
Y18602 Genomic DNA. Translation: CAC80089.1.
Y18603 Genomic DNA. Translation: CAC80090.1.
AF112086 Genomic DNA. Translation: AAF23561.1.
AB005230 Genomic DNA. Translation: BAB11121.1.
CP002688 Genomic DNA. Translation: AED91961.1.
AY044331 mRNA. Translation: AAK73272.1.
AY054278 mRNA. Translation: AAL06937.1.
AY058155 mRNA. Translation: AAL25571.1.
AY090376 mRNA. Translation: AAL91279.1.
AY087778 mRNA. Translation: AAM65314.1.
BT000596 mRNA. Translation: AAN18165.1.
Z17650 mRNA. Translation: CAA79023.1.
Z17649 mRNA. Translation: CAA79022.1.
PIRiA27721. SYMUCN.
RefSeqiNP_196897.1. NM_121396.4. [P13114-1]
UniGeneiAt.297.
At.69414.
At.71936.

3D structure databases

ProteinModelPortaliP13114.
SMRiP13114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16519. 8 interactors.
IntActiP13114. 6 interactors.
MINTiMINT-8184522.
STRINGi3702.AT5G13930.1.

PTM databases

iPTMnetiP13114.

Proteomic databases

PaxDbiP13114.
PRIDEiP13114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G13930.1; AT5G13930.1; AT5G13930. [P13114-1]
GeneIDi831241.
GrameneiAT5G13930.1; AT5G13930.1; AT5G13930.
KEGGiath:AT5G13930.

Organism-specific databases

TAIRiAT5G13930.

Phylogenomic databases

eggNOGiENOG410IKG2. Eukaryota.
COG3424. LUCA.
HOGENOMiHOG000245460.
InParanoidiP13114.
KOiK00660.
OMAiNICEYMA.
OrthoDBiEOG09360E8Q.
PhylomeDBiP13114.

Enzyme and pathway databases

UniPathwayiUPA00154.
BioCyciMetaCyc:AT5G13930-MONOMER.
BRENDAi2.3.1.74. 399.

Miscellaneous databases

PROiP13114.

Gene expression databases

ExpressionAtlasiP13114. baseline and differential.
GenevisibleiP13114. AT.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHSY_ARATH
AccessioniPrimary (citable) accession number: P13114
Secondary accession number(s): Q43132, Q940L6, Q941R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 30, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.