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Protein

Trypanothione reductase

Gene

TPR

Organism
Trypanosoma congolense
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Trypanothione is the parasite analog of glutathione; this enzyme is the equivalent of glutathione reductase.

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

Trypanothione + NADP+ = trypanothione disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Kineticsi

  1. KM=18 µM for trypanothione

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei461Proton acceptorBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi35 – 52FADBy similarityAdd BLAST18

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein, NADP

    Enzyme and pathway databases

    SABIO-RKiP13110

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypanothione reductase (EC:1.8.1.12)
    Short name:
    TR
    Alternative name(s):
    N(1),N(8)-bis(glutathionyl)spermidine reductase
    Gene namesi
    Name:TPR
    OrganismiTrypanosoma congolense
    Taxonomic identifieri5692 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaNannomonas

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi18E → A: KM=266 uM for trypanothione. 1
    Mutagenesisi21W → R: KM=600 uM for trypanothione. 1
    Mutagenesisi343A → R: KM=9 uM for trypanothione. 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000679911 – 492Trypanothione reductaseAdd BLAST492

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi52 ↔ 57Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP13110

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP13110
    SMRiP13110
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Redox-active center

    Family and domain databases

    Gene3Di3.30.390.30, 1 hit
    3.50.50.60, 3 hits
    InterProiView protein in InterPro
    IPR036188 FAD/NAD-bd_sf
    IPR023753 FAD/NAD-binding_dom
    IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
    IPR001100 Pyr_nuc-diS_OxRdtase
    IPR004099 Pyr_nucl-diS_OxRdtase_dimer
    IPR012999 Pyr_OxRdtase_I_AS
    IPR001864 Trypnth_redctse
    PfamiView protein in Pfam
    PF07992 Pyr_redox_2, 1 hit
    PF02852 Pyr_redox_dim, 1 hit
    PIRSFiPIRSF000350 Mercury_reductase_MerA, 1 hit
    PRINTSiPR00470 TRYPANRDTASE
    SUPFAMiSSF51905 SSF51905, 2 hits
    SSF55424 SSF55424, 1 hit
    TIGRFAMsiTIGR01423 trypano_reduc, 1 hit
    PROSITEiView protein in PROSITE
    PS00076 PYRIDINE_REDOX_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P13110-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKAFDLVII GAGSGGLEAG WNAATLYKKR VAVVDVQTVH GPPFFAALGG
    60 70 80 90 100
    TCVNVGCVPK KLMVTGAQYM DQLRESAGFG WEFDASTIKA NWKTLIAAKN
    110 120 130 140 150
    AAVLDINKSY EDMFKDTEGL EFFLGWGALE QKNVVTVREG ADPKSKVKER
    160 170 180 190 200
    LQAEHIIIAT GSWPQMLKIP GIEHCISSNE AFYLEEPPRR VLTVGGGFIS
    210 220 230 240 250
    VEFAGIFNAY KPVGGKVTLC YRNNPILRGF DYTLRQELTK QLVANGIDIM
    260 270 280 290 300
    TNENPSKIEL NPDGSKHVTF ESGKTLDVDV VMMAIGRLPR TGYLQLQTVG
    310 320 330 340 350
    VNLTDKGAIQ VDEFSRTNVP NIYAIGDVTG RIMLTPVAIN EGASVVDTIF
    360 370 380 390 400
    GSKPRKTDHT RVASAVFSIP PIGTCGLTEE EAAKSFEKVA VYLSCFTPLM
    410 420 430 440 450
    HNISGSKYKK FVAKIITDHG DGTVVGVHLL GDSSPEIIQA VGICMKLNAK
    460 470 480 490
    ISDFYNTIGV HPTSAEELCS MRTPSHYYIK GEKMETLPDS SL
    Length:492
    Mass (Da):53,443
    Last modified:January 1, 1990 - v1
    Checksum:i48F305A4C8C09685
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M21122 Genomic DNA Translation: AAA30258.1
    PIRiA27727

    Entry informationi

    Entry nameiTYTR_TRYCO
    AccessioniPrimary (citable) accession number: P13110
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: May 23, 2018
    This is version 115 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

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