P13109 (FGF2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparin-binding growth factor 2
Short name=HBGF-2

Alternative name(s):
Basic fibroblast growth factor
Short name=bFGF

Gene names

Name:	Fgf2
Synonyms:	Fgf-2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro By similarity.
Subunit structure	Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 By similarity.
Subcellular location	Secreted By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane By similarity.
Tissue specificity	Found in all tissues examined.
Post-translational modification	Phosphorylation at Tyr-81 regulates FGF2 unconventional secretion By similarity.
Sequence similarities	Belongs to the heparin-binding growth factors family.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Secreted
Ligand	Heparin-binding
Molecular function	Developmental protein Growth factor Mitogen
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from direct assay. Source: RGD MAPK import into nucleus Inferred from direct assay. Source: RGD branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB embryo development Inferred from mutant phenotype. Source: RGD fibroblast growth factor receptor signaling pathway Inferred from physical interaction. Source: MGI negative regulation of cell death Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell growth Inferred from direct assay. Source: RGD negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: RGD positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW regulation of calcium ion-dependent exocytosis Inferred from direct assay. Source: RGD synaptic transmission Inferred from direct assay. Source: RGD wound healing Inferred from expression pattern. Source: RGD
Cellular component	cytoplasm Inferred from direct assay. Source: RGD nucleus Inferred from direct assay. Source: RGD
Molecular function	fibroblast growth factor binding Inferred from physical interaction. Source: RGD fibroblast growth factor receptor binding Inferred from physical interaction. Source: MGI growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW heparin binding Inferred from electronic annotation. Source: UniProtKB-KW nerve growth factor receptor binding Traceable author statement. Source: RGD voltage-gated calcium channel activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 9	9			PRO_0000008938
	Chain	10 – 154	145	Heparin-binding growth factor 2		PRO_0000008939
Regions
	Region	127 – 143	17	Heparin-binding By similarity
Sites
	Binding site	35	1	Heparin By similarity
Amino acid modifications
	Modified residue	81	1	Phosphotyrosine; by TEC By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13109 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 1A0F14FF423D8403	FASTA	154	17,139
	10 20 30 40 50 60 MAAGSITSLP ALPEDGGGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK 70 80 90 100 110 120 LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS 130 140 150 SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

References

[1]	"Complementary DNA cloning and sequencing of rat ovarian basic fibroblast growth factor and tissue distribution study of its mRNA." Shimasaki S., Emoto N., Koba A., Mercado M., Shibata F., Cooksey K., Baird A., Ling N. Biochem. Biophys. Res. Commun. 157:256-263(1988) [PubMed: 3196337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Ovary.
[2]	"Nucleotide sequence of rat basic fibroblast growth factor cDNA." Kurokawa T., Seno M., Igarashi K. Nucleic Acids Res. 16:5201-5201(1988) [PubMed: 3387229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Cloning of the rat fibroblast growth factor-2 promoter region and its response to mitogenic stimuli in glioma C6 cells." Pasumarthi K.B.S., Jin Y., Cattini P.A. J. Neurochem. 68:898-908(1997) [PubMed: 9048734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28. Strain: Sprague-Dawley. Tissue: Testis.
[4]	"PCR detection of the rat brain basic fibroblast growth factor (bFGF) mRNA containing a unique 3' untranslated region." El-Husseini A.E.-D., Paterson J.A., Myal Y., Shiu R.P.C. Biochim. Biophys. Acta 1131:314-316(1992) [PubMed: 1378302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-154. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22427 mRNA. Translation: AAA41210.1. X07285 mRNA. Translation: CAA30265.1. U78079 Genomic DNA. Translation: AAC53225.1. X61697 mRNA. Translation: CAA43863.1.
IPI	IPI00198326.
PIR	A31674.
RefSeq	NP_062178.1. NM_019305.2.
UniGene	Rn.31808.
3D structure databases
ProteinModelPortal	P13109.
SMR	P13109. Positions 1-154.
ModBase	Search...
Protein-protein interaction databases
STRING	P13109.
PTM databases
PhosphoSite	P13109.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000023388; ENSRNOP00000023388; ENSRNOG00000017392.
GeneID	54250.
KEGG	rno:54250.
UCSC	NM_019305. rat.
Organism-specific databases
CTD	2247.
RGD	2609. Fgf2.
Phylogenomic databases
GeneTree	ENSGT00600000084030.
HOVERGEN	HBG007580.
InParanoid	P13109.
OMA	YRSRKYP.
OrthoDB	EOG4K0QPR.
PhylomeDB	P13109.
Gene expression databases
ArrayExpress	P13109.
Genevestigator	P13109.
GermOnline	ENSRNOG00000017392. Rattus norvegicus.
Family and domain databases
InterPro	IPR008996. Cytokine_IL1-like. IPR002209. GF_heparin-bd. IPR002348. IL1_HBGF. [Graphical view]
KO	K04358.
PANTHER	PTHR11486. IL1_HBGF. 1 hit.
Pfam	PF00167. FGF. 1 hit. [Graphical view]
PRINTS	PR00263. HBGFFGF. PR00262. IL1HBGF.
SMART	SM00442. FGF. 1 hit. [Graphical view]
SUPFAM	SSF50353. Cytok_IL1_like. 1 hit.
PROSITE	PS00247. HBGF_FGF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	610748.

Entry information

Entry name

FGF2_RAT

Accession

Primary (citable) accession number: P13109

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	November 16, 2011
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program