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P13108

- CP2D4_RAT

UniProt

P13108 - CP2D4_RAT

Protein

Cytochrome P450 2D4

Gene

Cyp2d4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi446 – 4461Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. arachidonic acid monooxygenase activity Source: RGD
    2. aromatase activity Source: UniProtKB-EC
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. monooxygenase activity Source: RGD
    6. steroid 21-monooxygenase activity Source: RGD

    GO - Biological processi

    1. arachidonic acid metabolic process Source: RGD
    2. dopamine biosynthetic process Source: RGD
    3. dopamine metabolic process Source: RGD
    4. drug metabolic process Source: RGD
    5. female pregnancy Source: RGD
    6. response to organic substance Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP13108.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2D4 (EC:1.14.14.1)
    Alternative name(s):
    CYPIID4
    Cytochrome P450-CMF3
    Cytochrome P450-DB4
    Debrisoquine 4-hydroxylase
    Gene namesi
    Name:Cyp2d4
    Synonyms:Cyp2d-4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620640. Cyp2d4.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Cytochrome P450 2D4PRO_0000051730Add
    BLAST

    Proteomic databases

    PaxDbiP13108.
    PRIDEiP13108.

    Expressioni

    Inductioni

    P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

    Gene expression databases

    GenevestigatoriP13108.

    Structurei

    3D structure databases

    ProteinModelPortaliP13108.
    SMRiP13108. Positions 37-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036991.
    HOVERGENiHBG015789.
    InParanoidiP13108.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01686. EP450ICYP2D.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13108-1 [UniParc]FASTAAdd to Basket

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    MRMPTGSELW PIAIFTIIFL LLVDLMHRRQ RWTSRYPPGP VPWPVLGNLL    50
    QIDFQNMPAG FQKLRCRFGD LFSLQLAFES VVVLNGLPAL REALVKYSED 100
    TADRPPLHFN DQSGFGPRSQ GVVLARYGPA WRQQRRFSVS TFRHFGLGKK 150
    SLEQWVTEEA RCLCAAFADH SGFPFSPNTL LDKAVCNVIA SLLFACRFEY 200
    NDPRFIRLLD LLKDTLEEES GFLPMLLNVF PMLLHIPGLL GKVFSGKKAF 250
    VAMLDELLTE HKVTWDPAQP PRDLTDAFLA EVEKAKGNPE SSFNDENLRV 300
    VVADLFMAGM VTTSTTLTWA LLFMILHPDV QCRVQQEIDE VIGQVRRPEM 350
    ADQARMPFTN AVIHEVQRFA DILPLGVPHK TSRDIEVQGF LIPKGTTLIT 400
    NLSSVLKDET VWEKPLRFHP EHFLDAQGNF VKHEAFMPFS AGRRACLGEP 450
    LARMELFLFF TCLLQRFSFS VPTGQPRPSD YGIFGALTTP RPYQLCASPR 500
    Length:500
    Mass (Da):56,698
    Last modified:March 1, 1992 - v2
    Checksum:i505D29B2C0BB1F7E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti473 – 4731T → A in BAA23125. (PubMed:9434752)Curated
    Sequence conflicti480 – 4801D → N in BAA23125. (PubMed:9434752)Curated
    Sequence conflicti483 – 4831I → V in BAA23125. (PubMed:9434752)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52029 Genomic DNA. Translation: CAA36271.1.
    AB008425 mRNA. Translation: BAA23125.1.
    M22331 mRNA. Translation: AAA41052.1.
    PIRiS16873. D31579.
    UniGeneiRn.26060.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52029 Genomic DNA. Translation: CAA36271.1 .
    AB008425 mRNA. Translation: BAA23125.1 .
    M22331 mRNA. Translation: AAA41052.1 .
    PIRi S16873. D31579.
    UniGenei Rn.26060.

    3D structure databases

    ProteinModelPortali P13108.
    SMRi P13108. Positions 37-500.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL2304403.

    Proteomic databases

    PaxDbi P13108.
    PRIDEi P13108.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 620640. Cyp2d4.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036991.
    HOVERGENi HBG015789.
    InParanoidi P13108.

    Enzyme and pathway databases

    SABIO-RK P13108.

    Gene expression databases

    Genevestigatori P13108.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01686. EP450ICYP2D.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site."
      Matsunaga E., Umeno M., Gonzalez F.J.
      J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity."
      Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y.
      Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. "Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily."
      Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S.
      Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-500.

    Entry informationi

    Entry nameiCP2D4_RAT
    AccessioniPrimary (citable) accession number: P13108
    Secondary accession number(s): O35107
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3