P13108 (CP2D4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2D4
EC=1.14.14.1

Alternative name(s):
CYPIID4
Cytochrome P450-CMF3
Cytochrome P450-DB4
Debrisoquine 4-hydroxylase

Gene names

Name:	Cyp2d4
Synonyms:	Cyp2d-4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Cytochrome P450 2D4		PRO_0000051730
Sites
	Metal binding	446	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	473	1	T → A in BAA23125. Ref.2
	Sequence conflict	480	1	D → N in BAA23125. Ref.2
	Sequence conflict	483	1	I → V in BAA23125. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P13108 [UniParc]. Last modified March 1, 1992. Version 2. Checksum: 505D29B2C0BB1F7E	FASTA	500	56,698
	10 20 30 40 50 60 MRMPTGSELW PIAIFTIIFL LLVDLMHRRQ RWTSRYPPGP VPWPVLGNLL QIDFQNMPAG 70 80 90 100 110 120 FQKLRCRFGD LFSLQLAFES VVVLNGLPAL REALVKYSED TADRPPLHFN DQSGFGPRSQ 130 140 150 160 170 180 GVVLARYGPA WRQQRRFSVS TFRHFGLGKK SLEQWVTEEA RCLCAAFADH SGFPFSPNTL 190 200 210 220 230 240 LDKAVCNVIA SLLFACRFEY NDPRFIRLLD LLKDTLEEES GFLPMLLNVF PMLLHIPGLL 250 260 270 280 290 300 GKVFSGKKAF VAMLDELLTE HKVTWDPAQP PRDLTDAFLA EVEKAKGNPE SSFNDENLRV 310 320 330 340 350 360 VVADLFMAGM VTTSTTLTWA LLFMILHPDV QCRVQQEIDE VIGQVRRPEM ADQARMPFTN 370 380 390 400 410 420 AVIHEVQRFA DILPLGVPHK TSRDIEVQGF LIPKGTTLIT NLSSVLKDET VWEKPLRFHP 430 440 450 460 470 480 EHFLDAQGNF VKHEAFMPFS AGRRACLGEP LARMELFLFF TCLLQRFSFS VPTGQPRPSD 490 500 YGIFGALTTP RPYQLCASPR

References

[1]	"The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site." Matsunaga E., Umeno M., Gonzalez F.J. J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity." Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y. Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Brain.
[3]	"Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily." Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S. Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-500.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52029 Genomic DNA. Translation: CAA36271.1. AB008425 mRNA. Translation: BAA23125.1. M22331 mRNA. Translation: AAA41052.1.
IPI	IPI00198325.
PIR	D31579. S16873.
UniGene	Rn.26060.
3D structure databases
ProteinModelPortal	P13108.
SMR	P13108. Positions 37-500.
ModBase	Search...
Proteomic databases
PaxDb	P13108.
PRIDE	P13108.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
InParanoid	P13108.
OrthoDB	EOG40VVPH.
Enzyme and pathway databases
SABIO-RK	P13108.
Gene expression databases
ArrayExpress	P13108.
Genevestigator	P13108.
GermOnline	ENSRNOG00000032261. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2D4_RAT

Accession

Primary (citable) accession number: P13108
Secondary accession number(s): O35107

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program