Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P13108 (CP2D4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2D4

EC=1.14.14.1
Alternative name(s):
CYPIID4
Cytochrome P450-CMF3
Cytochrome P450-DB4
Debrisoquine 4-hydroxylase
Gene names
Name:Cyp2d4
Synonyms:Cyp2d-4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Cytochrome P450 2D4
PRO_0000051730

Sites

Metal binding4461Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict4731T → A in BAA23125. Ref.2
Sequence conflict4801D → N in BAA23125. Ref.2
Sequence conflict4831I → V in BAA23125. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P13108 [UniParc].

Last modified March 1, 1992. Version 2.
Checksum: 505D29B2C0BB1F7E

FASTA50056,698
        10         20         30         40         50         60 
MRMPTGSELW PIAIFTIIFL LLVDLMHRRQ RWTSRYPPGP VPWPVLGNLL QIDFQNMPAG 

        70         80         90        100        110        120 
FQKLRCRFGD LFSLQLAFES VVVLNGLPAL REALVKYSED TADRPPLHFN DQSGFGPRSQ 

       130        140        150        160        170        180 
GVVLARYGPA WRQQRRFSVS TFRHFGLGKK SLEQWVTEEA RCLCAAFADH SGFPFSPNTL 

       190        200        210        220        230        240 
LDKAVCNVIA SLLFACRFEY NDPRFIRLLD LLKDTLEEES GFLPMLLNVF PMLLHIPGLL 

       250        260        270        280        290        300 
GKVFSGKKAF VAMLDELLTE HKVTWDPAQP PRDLTDAFLA EVEKAKGNPE SSFNDENLRV 

       310        320        330        340        350        360 
VVADLFMAGM VTTSTTLTWA LLFMILHPDV QCRVQQEIDE VIGQVRRPEM ADQARMPFTN 

       370        380        390        400        410        420 
AVIHEVQRFA DILPLGVPHK TSRDIEVQGF LIPKGTTLIT NLSSVLKDET VWEKPLRFHP 

       430        440        450        460        470        480 
EHFLDAQGNF VKHEAFMPFS AGRRACLGEP LARMELFLFF TCLLQRFSFS VPTGQPRPSD 

       490        500 
YGIFGALTTP RPYQLCASPR 

« Hide

References

[1]"The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site."
Matsunaga E., Umeno M., Gonzalez F.J.
J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity."
Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y.
Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[3]"Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily."
Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S.
Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-500.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52029 Genomic DNA. Translation: CAA36271.1.
AB008425 mRNA. Translation: BAA23125.1.
M22331 mRNA. Translation: AAA41052.1.
PIRD31579. S16873.
UniGeneRn.26060.

3D structure databases

ProteinModelPortalP13108.
SMRP13108. Positions 37-500.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL2304403.

Proteomic databases

PaxDbP13108.
PRIDEP13108.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD620640. Cyp2d4.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG015789.
InParanoidP13108.

Enzyme and pathway databases

SABIO-RKP13108.

Gene expression databases

GenevestigatorP13108.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP2D4_RAT
AccessionPrimary (citable) accession number: P13108
Secondary accession number(s): O35107
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families