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Protein

Cytochrome P450 2B3

Gene

Cyp2b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

  • epoxygenase P450 pathway Source: GO_Central
  • exogenous drug catabolic process Source: GO_Central
  • oxidation-reduction process Source: GO_Central
  • response to drug Source: RGD
  • response to organic cyclic compound Source: RGD
  • xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_315621. Xenobiotics.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B3 (EC:1.14.14.1)
Alternative name(s):
CYPIIB3
Gene namesi
Name:Cyp2b3
Synonyms:Cyp2b-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi628627. Cyp2b3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Cytochrome P450 2B3PRO_0000051680Add
BLAST

Proteomic databases

PaxDbiP13107.
PRIDEiP13107.

Expressioni

Tissue specificityi

Liver. Not found in the lung, kidney and prostate.

Inductioni

Constitutively expressed.

Gene expression databases

ExpressionAtlasiP13107. baseline and differential.
GenevisibleiP13107. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4996448.
STRINGi10116.ENSRNOP00000030069.

Structurei

3D structure databases

ProteinModelPortaliP13107.
SMRiP13107. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP13107.
KOiK07412.
OrthoDBiEOG7RBZ85.
PhylomeDBiP13107.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSVLLLLA VLLSFLLFLV RGHAKVHGHL PPGPRPLPLL GNLLQMDRGG
60 70 80 90 100
FRKSFIQLQE KHGDVFTVYF GPRPVVMLCG TQTIREALVD HAEAFSGRGI
110 120 130 140 150
IAVLQPIMQE YGVSFVNEER WKILRRLFVA TMRDFGIGKQ SVEDQIKEEA
160 170 180 190 200
KCLVEELKNH QGVSLDPTFL FQCVTGNIIC SIVFGERFDY RDRQFLRLLD
210 220 230 240 250
LLYRTFSLIS SFSSQMFEVY SDFLKYFPGV HREIYKNLKE VLDYIDHSVE
260 270 280 290 300
NHRATLDPNA PRDFIDTFLL HMEKEKLNHY TEFHHWNLMI SVLFLFLAGT
310 320 330 340 350
ESTSNTLCYG FLLMLKYPHV AEKVQKEIDQ VIGSQRVPTL DDRSKMPYTE
360 370 380 390 400
AVIHEIQRFS DVSPMGLPCR ITKDTLFRGY LLPKNTEVYF ILSSALHDPQ
410 420 430 440 450
YFEQPDTFNP EHFLDANGAL KKCEAFMPFS IGKRMCLGEG IARSELFLFF
460 470 480 490
TTILQNYSVS SPVDPNTIDM TPKESGLAKV APVYKICFVA R
Length:491
Mass (Da):56,384
Last modified:January 1, 1990 - v1
Checksum:i0B5ACB77577AAF29
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti364 – 3641P → Q in AAA41048 (PubMed:3013548).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20406 mRNA. Translation: AAA41006.1.
BC088103 mRNA. Translation: AAH88103.1.
BC105823 mRNA. Translation: AAI05824.1.
BC127479 mRNA. Translation: AAI27480.1.
M19973 mRNA. Translation: AAA41048.1.
PIRiA29818.
RefSeqiNP_775416.1. NM_173294.2.
UniGeneiRn.234313.
Rn.4845.

Genome annotation databases

EnsembliENSRNOT00000034845; ENSRNOP00000030069; ENSRNOG00000033164.
GeneIDi286953.
KEGGirno:286953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20406 mRNA. Translation: AAA41006.1.
BC088103 mRNA. Translation: AAH88103.1.
BC105823 mRNA. Translation: AAI05824.1.
BC127479 mRNA. Translation: AAI27480.1.
M19973 mRNA. Translation: AAA41048.1.
PIRiA29818.
RefSeqiNP_775416.1. NM_173294.2.
UniGeneiRn.234313.
Rn.4845.

3D structure databases

ProteinModelPortaliP13107.
SMRiP13107. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996448.
STRINGi10116.ENSRNOP00000030069.

Proteomic databases

PaxDbiP13107.
PRIDEiP13107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000034845; ENSRNOP00000030069; ENSRNOG00000033164.
GeneIDi286953.
KEGGirno:286953.

Organism-specific databases

CTDi286953.
RGDi628627. Cyp2b3.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP13107.
KOiK07412.
OrthoDBiEOG7RBZ85.
PhylomeDBiP13107.

Enzyme and pathway databases

ReactomeiREACT_315621. Xenobiotics.

Miscellaneous databases

NextBioi625136.
PROiP13107.

Gene expression databases

ExpressionAtlasiP13107. baseline and differential.
GenevisibleiP13107. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A constitutive member of the rat cytochrome P450IIB subfamily: full-length coding sequence of the P450IIB3 cDNA."
    Labbe D., Jean A., Anderson A.
    DNA 7:253-260(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  3. "cDNA clones for liver cytochrome P-450s from individual aroclor-treated rats: constitutive expression of a new P-450 gene related to phenobarbital-inducible forms."
    Affolter M., Labbe D., Jean A., Raymond M., Noel D., Labelle Y., Parent-Vaugeois C., Lambert M., Bojanowski R., Anderson A.
    DNA 5:209-218(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 295-491.

Entry informationi

Entry nameiCP2B3_RAT
AccessioniPrimary (citable) accession number: P13107
Secondary accession number(s): A1L121, Q3B8R5, Q64585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 24, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.