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P13107

- CP2B3_RAT

UniProt

P13107 - CP2B3_RAT

Protein

Cytochrome P450 2B3

Gene

Cyp2b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi436 – 4361Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: RGD

    GO - Biological processi

    1. response to drug Source: RGD
    2. response to organic cyclic compound Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2B3 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIB3
    Gene namesi
    Name:Cyp2b3
    Synonyms:Cyp2b-3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi628627. Cyp2b3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Cytochrome P450 2B3PRO_0000051680Add
    BLAST

    Proteomic databases

    PaxDbiP13107.
    PRIDEiP13107.

    Expressioni

    Tissue specificityi

    Liver. Not found in the lung, kidney and prostate.

    Inductioni

    Constitutively expressed.

    Gene expression databases

    GenevestigatoriP13107.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4996448.

    Structurei

    3D structure databases

    ProteinModelPortaliP13107.
    SMRiP13107. Positions 28-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00670000097868.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    KOiK07412.
    OMAiASINDYY.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP13107.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01685. EP450ICYP2B.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13107-1 [UniParc]FASTAAdd to Basket

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    MDTSVLLLLA VLLSFLLFLV RGHAKVHGHL PPGPRPLPLL GNLLQMDRGG    50
    FRKSFIQLQE KHGDVFTVYF GPRPVVMLCG TQTIREALVD HAEAFSGRGI 100
    IAVLQPIMQE YGVSFVNEER WKILRRLFVA TMRDFGIGKQ SVEDQIKEEA 150
    KCLVEELKNH QGVSLDPTFL FQCVTGNIIC SIVFGERFDY RDRQFLRLLD 200
    LLYRTFSLIS SFSSQMFEVY SDFLKYFPGV HREIYKNLKE VLDYIDHSVE 250
    NHRATLDPNA PRDFIDTFLL HMEKEKLNHY TEFHHWNLMI SVLFLFLAGT 300
    ESTSNTLCYG FLLMLKYPHV AEKVQKEIDQ VIGSQRVPTL DDRSKMPYTE 350
    AVIHEIQRFS DVSPMGLPCR ITKDTLFRGY LLPKNTEVYF ILSSALHDPQ 400
    YFEQPDTFNP EHFLDANGAL KKCEAFMPFS IGKRMCLGEG IARSELFLFF 450
    TTILQNYSVS SPVDPNTIDM TPKESGLAKV APVYKICFVA R 491
    Length:491
    Mass (Da):56,384
    Last modified:January 1, 1990 - v1
    Checksum:i0B5ACB77577AAF29
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti364 – 3641P → Q in AAA41048. (PubMed:3013548)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20406 mRNA. Translation: AAA41006.1.
    BC088103 mRNA. Translation: AAH88103.1.
    BC105823 mRNA. Translation: AAI05824.1.
    BC127479 mRNA. Translation: AAI27480.1.
    M19973 mRNA. Translation: AAA41048.1.
    PIRiA29818.
    RefSeqiNP_775416.1. NM_173294.2.
    UniGeneiRn.234313.
    Rn.4845.

    Genome annotation databases

    EnsembliENSRNOT00000034845; ENSRNOP00000030069; ENSRNOG00000033164.
    GeneIDi286953.
    KEGGirno:286953.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20406 mRNA. Translation: AAA41006.1 .
    BC088103 mRNA. Translation: AAH88103.1 .
    BC105823 mRNA. Translation: AAI05824.1 .
    BC127479 mRNA. Translation: AAI27480.1 .
    M19973 mRNA. Translation: AAA41048.1 .
    PIRi A29818.
    RefSeqi NP_775416.1. NM_173294.2.
    UniGenei Rn.234313.
    Rn.4845.

    3D structure databases

    ProteinModelPortali P13107.
    SMRi P13107. Positions 28-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4996448.

    Proteomic databases

    PaxDbi P13107.
    PRIDEi P13107.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000034845 ; ENSRNOP00000030069 ; ENSRNOG00000033164 .
    GeneIDi 286953.
    KEGGi rno:286953.

    Organism-specific databases

    CTDi 286953.
    RGDi 628627. Cyp2b3.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00670000097868.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    KOi K07412.
    OMAi ASINDYY.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P13107.

    Miscellaneous databases

    NextBioi 625136.

    Gene expression databases

    Genevestigatori P13107.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01685. EP450ICYP2B.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A constitutive member of the rat cytochrome P450IIB subfamily: full-length coding sequence of the P450IIB3 cDNA."
      Labbe D., Jean A., Anderson A.
      DNA 7:253-260(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    3. "cDNA clones for liver cytochrome P-450s from individual aroclor-treated rats: constitutive expression of a new P-450 gene related to phenobarbital-inducible forms."
      Affolter M., Labbe D., Jean A., Raymond M., Noel D., Labelle Y., Parent-Vaugeois C., Lambert M., Bojanowski R., Anderson A.
      DNA 5:209-218(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 295-491.

    Entry informationi

    Entry nameiCP2B3_RAT
    AccessioniPrimary (citable) accession number: P13107
    Secondary accession number(s): A1L121, Q3B8R5, Q64585
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3