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P13107

- CP2B3_RAT

UniProt

P13107 - CP2B3_RAT

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Protein

Cytochrome P450 2B3

Gene

Cyp2b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. monooxygenase activity Source: RGD

GO - Biological processi

  1. response to drug Source: RGD
  2. response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_261605. Xenobiotics.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B3 (EC:1.14.14.1)
Alternative name(s):
CYPIIB3
Gene namesi
Name:Cyp2b3
Synonyms:Cyp2b-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi628627. Cyp2b3.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. intracellular membrane-bounded organelle Source: RGD
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Cytochrome P450 2B3PRO_0000051680Add
BLAST

Proteomic databases

PaxDbiP13107.
PRIDEiP13107.

Expressioni

Tissue specificityi

Liver. Not found in the lung, kidney and prostate.

Inductioni

Constitutively expressed.

Gene expression databases

ExpressionAtlasiP13107. baseline and differential.
GenevestigatoriP13107.

Interactioni

Protein-protein interaction databases

MINTiMINT-4996448.

Structurei

3D structure databases

ProteinModelPortaliP13107.
SMRiP13107. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP13107.
KOiK07412.
OMAiASINDYY.
OrthoDBiEOG7RBZ85.
PhylomeDBiP13107.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13107-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDTSVLLLLA VLLSFLLFLV RGHAKVHGHL PPGPRPLPLL GNLLQMDRGG
60 70 80 90 100
FRKSFIQLQE KHGDVFTVYF GPRPVVMLCG TQTIREALVD HAEAFSGRGI
110 120 130 140 150
IAVLQPIMQE YGVSFVNEER WKILRRLFVA TMRDFGIGKQ SVEDQIKEEA
160 170 180 190 200
KCLVEELKNH QGVSLDPTFL FQCVTGNIIC SIVFGERFDY RDRQFLRLLD
210 220 230 240 250
LLYRTFSLIS SFSSQMFEVY SDFLKYFPGV HREIYKNLKE VLDYIDHSVE
260 270 280 290 300
NHRATLDPNA PRDFIDTFLL HMEKEKLNHY TEFHHWNLMI SVLFLFLAGT
310 320 330 340 350
ESTSNTLCYG FLLMLKYPHV AEKVQKEIDQ VIGSQRVPTL DDRSKMPYTE
360 370 380 390 400
AVIHEIQRFS DVSPMGLPCR ITKDTLFRGY LLPKNTEVYF ILSSALHDPQ
410 420 430 440 450
YFEQPDTFNP EHFLDANGAL KKCEAFMPFS IGKRMCLGEG IARSELFLFF
460 470 480 490
TTILQNYSVS SPVDPNTIDM TPKESGLAKV APVYKICFVA R
Length:491
Mass (Da):56,384
Last modified:January 1, 1990 - v1
Checksum:i0B5ACB77577AAF29
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti364 – 3641P → Q in AAA41048. (PubMed:3013548)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20406 mRNA. Translation: AAA41006.1.
BC088103 mRNA. Translation: AAH88103.1.
BC105823 mRNA. Translation: AAI05824.1.
BC127479 mRNA. Translation: AAI27480.1.
M19973 mRNA. Translation: AAA41048.1.
PIRiA29818.
RefSeqiNP_775416.1. NM_173294.2.
UniGeneiRn.234313.
Rn.4845.

Genome annotation databases

EnsembliENSRNOT00000034845; ENSRNOP00000030069; ENSRNOG00000033164.
GeneIDi286953.
KEGGirno:286953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20406 mRNA. Translation: AAA41006.1 .
BC088103 mRNA. Translation: AAH88103.1 .
BC105823 mRNA. Translation: AAI05824.1 .
BC127479 mRNA. Translation: AAI27480.1 .
M19973 mRNA. Translation: AAA41048.1 .
PIRi A29818.
RefSeqi NP_775416.1. NM_173294.2.
UniGenei Rn.234313.
Rn.4845.

3D structure databases

ProteinModelPortali P13107.
SMRi P13107. Positions 28-491.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4996448.

Proteomic databases

PaxDbi P13107.
PRIDEi P13107.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000034845 ; ENSRNOP00000030069 ; ENSRNOG00000033164 .
GeneIDi 286953.
KEGGi rno:286953.

Organism-specific databases

CTDi 286953.
RGDi 628627. Cyp2b3.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000118775.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.
InParanoidi P13107.
KOi K07412.
OMAi ASINDYY.
OrthoDBi EOG7RBZ85.
PhylomeDBi P13107.

Enzyme and pathway databases

Reactomei REACT_261605. Xenobiotics.

Miscellaneous databases

NextBioi 625136.

Gene expression databases

ExpressionAtlasi P13107. baseline and differential.
Genevestigatori P13107.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A constitutive member of the rat cytochrome P450IIB subfamily: full-length coding sequence of the P450IIB3 cDNA."
    Labbe D., Jean A., Anderson A.
    DNA 7:253-260(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  3. "cDNA clones for liver cytochrome P-450s from individual aroclor-treated rats: constitutive expression of a new P-450 gene related to phenobarbital-inducible forms."
    Affolter M., Labbe D., Jean A., Raymond M., Noel D., Labelle Y., Parent-Vaugeois C., Lambert M., Bojanowski R., Anderson A.
    DNA 5:209-218(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 295-491.

Entry informationi

Entry nameiCP2B3_RAT
AccessioniPrimary (citable) accession number: P13107
Secondary accession number(s): A1L121, Q3B8R5, Q64585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 26, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3