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P13107 (CP2B3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2B3

EC=1.14.14.1
Alternative name(s):
CYPIIB3
Gene names
Name:Cyp2b3
Synonyms:Cyp2b-3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver. Not found in the lung, kidney and prostate.

Induction

Constitutively expressed.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Cytochrome P450 2B3
PRO_0000051680

Sites

Metal binding4361Iron (heme axial ligand)

Experimental info

Sequence conflict3641P → Q in AAA41048. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P13107 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 0B5ACB77577AAF29

FASTA49156,384
        10         20         30         40         50         60 
MDTSVLLLLA VLLSFLLFLV RGHAKVHGHL PPGPRPLPLL GNLLQMDRGG FRKSFIQLQE 

        70         80         90        100        110        120 
KHGDVFTVYF GPRPVVMLCG TQTIREALVD HAEAFSGRGI IAVLQPIMQE YGVSFVNEER 

       130        140        150        160        170        180 
WKILRRLFVA TMRDFGIGKQ SVEDQIKEEA KCLVEELKNH QGVSLDPTFL FQCVTGNIIC 

       190        200        210        220        230        240 
SIVFGERFDY RDRQFLRLLD LLYRTFSLIS SFSSQMFEVY SDFLKYFPGV HREIYKNLKE 

       250        260        270        280        290        300 
VLDYIDHSVE NHRATLDPNA PRDFIDTFLL HMEKEKLNHY TEFHHWNLMI SVLFLFLAGT 

       310        320        330        340        350        360 
ESTSNTLCYG FLLMLKYPHV AEKVQKEIDQ VIGSQRVPTL DDRSKMPYTE AVIHEIQRFS 

       370        380        390        400        410        420 
DVSPMGLPCR ITKDTLFRGY LLPKNTEVYF ILSSALHDPQ YFEQPDTFNP EHFLDANGAL 

       430        440        450        460        470        480 
KKCEAFMPFS IGKRMCLGEG IARSELFLFF TTILQNYSVS SPVDPNTIDM TPKESGLAKV 

       490 
APVYKICFVA R 

« Hide

References

« Hide 'large scale' references
[1]"A constitutive member of the rat cytochrome P450IIB subfamily: full-length coding sequence of the P450IIB3 cDNA."
Labbe D., Jean A., Anderson A.
DNA 7:253-260(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[3]"cDNA clones for liver cytochrome P-450s from individual aroclor-treated rats: constitutive expression of a new P-450 gene related to phenobarbital-inducible forms."
Affolter M., Labbe D., Jean A., Raymond M., Noel D., Labelle Y., Parent-Vaugeois C., Lambert M., Bojanowski R., Anderson A.
DNA 5:209-218(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 295-491.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20406 mRNA. Translation: AAA41006.1.
BC088103 mRNA. Translation: AAH88103.1.
BC105823 mRNA. Translation: AAI05824.1.
BC127479 mRNA. Translation: AAI27480.1.
M19973 mRNA. Translation: AAA41048.1.
PIRA29818.
RefSeqNP_775416.1. NM_173294.2.
UniGeneRn.234313.
Rn.4845.

3D structure databases

ProteinModelPortalP13107.
SMRP13107. Positions 28-491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996448.

Proteomic databases

PaxDbP13107.
PRIDEP13107.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000034845; ENSRNOP00000030069; ENSRNOG00000033164.
GeneID286953.
KEGGrno:286953.

Organism-specific databases

CTD286953.
RGD628627. Cyp2b3.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00670000097868.
HOGENOMHOG000036992.
HOVERGENHBG015789.
KOK07412.
OrthoDBEOG7RBZ85.
PhylomeDBP13107.

Gene expression databases

GenevestigatorP13107.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio625136.

Entry information

Entry nameCP2B3_RAT
AccessionPrimary (citable) accession number: P13107
Secondary accession number(s): A1L121, Q3B8R5, Q64585
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families