Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P13099

- TOP3_YEAST

UniProt

P13099 - TOP3_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA topoisomerase 3

Gene

TOP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Essential for proper chromosome segregation in both meiosis and mitosis. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings.By similarity1 Publication

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei356 – 3561O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase activity Source: SGD
  3. DNA topoisomerase type I activity Source: SGD

GO - Biological processi

  1. DNA topological change Source: SGD
  2. mitotic sister chromatid cohesion Source: SGD
  3. reciprocal meiotic recombination Source: SGD
  4. regulation of DNA recombination Source: SGD
  5. telomere maintenance via telomerase Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32345-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III
Gene namesi
Name:TOP3
Synonyms:EDR1
Ordered Locus Names:YLR234W
ORF Names:L8083.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR234w.
SGDiS000004224. TOP3.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
  2. RecQ helicase-Topo III complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 656656DNA topoisomerase 3PRO_0000145198Add
BLAST

Proteomic databases

MaxQBiP13099.
PaxDbiP13099.
PeptideAtlasiP13099.

Expressioni

Gene expression databases

GenevestigatoriP13099.

Interactioni

Subunit structurei

Forms a complex with SGS1 and RMI1. Interacts with SGS1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SGS1P351873EBI-19365,EBI-17059

Protein-protein interaction databases

BioGridi31503. 106 interactions.
DIPiDIP-2912N.
IntActiP13099. 3 interactions.
MINTiMINT-442624.
STRINGi4932.YLR234W.

Structurei

3D structure databases

ProteinModelPortaliP13099.
SMRiP13099. Positions 2-633.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 156155ToprimPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0550.
GeneTreeiENSGT00530000063430.
HOGENOMiHOG000184377.
InParanoidiP13099.
KOiK03165.
OMAiARFSNTE.
OrthoDBiEOG7Q8CWW.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13099-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLCVAEKN SIAKAVSQIL GGGRSTSRDS GYMYVKNYDF MFSGFPFARN
60 70 80 90 100
GANCEVTMTS VAGHLTGIDF SHDSHGWGKC AIQELFDAPL NEIMNNNQKK
110 120 130 140 150
IASNIKREAR NADYLMIWTD CDREGEYIGW EIWQEAKRGN RLIQNDQVYR
160 170 180 190 200
AVFSHLERQH ILNAARNPSR LDMKSVHAVG TRIEIDLRAG VTFTRLLTET
210 220 230 240 250
LRNKLRNQAT MTKDGAKHRG GNKNDSQVVS YGTCQFPTLG FVVDRFERIR
260 270 280 290 300
NFVPEEFWYI QLVVENKDNG GTTTFQWDRG HLFDRLSVLT FYETCIETAG
310 320 330 340 350
NVAQVVDLKS KPTTKYRPLP LTTVELQKNC ARYLRLNAKQ SLDAAEKLYQ
360 370 380 390 400
KGFISYPRTE TDTFPHAMDL KSLVEKQAQL DQLAAGGRTA WASYAASLLQ
410 420 430 440 450
PENTSNNNKF KFPRSGSHDD KAHPPIHPIV SLGPEANVSP VERRVYEYVA
460 470 480 490 500
RHFLACCSED AKGQSMTLVL DWAVERFSAS GLVVLERNFL DVYPWARWET
510 520 530 540 550
TKQLPRLEMN ALVDIAKAEM KAGTTAPPKP MTESELILLM DTNGIGTDAT
560 570 580 590 600
IAEHIDKIQV RNYVRSEKVG KETYLQPTTL GVSLVHGFEA IGLEDSFAKP
610 620 630 640 650
FQRREMEQDL KKICEGHASK TDVVKDIVEK YRKYWHKTNA CKNTLLQVYD

RVKASM
Length:656
Mass (Da):74,371
Last modified:January 1, 1990 - v1
Checksum:i51DF78936A88B4F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24939 Genomic DNA. Translation: AAA35161.1.
U19027 Genomic DNA. Translation: AAB67406.1.
BK006945 Genomic DNA. Translation: DAA09550.1.
PIRiA33169. ISBYT3.
RefSeqiNP_013335.1. NM_001182121.1.

Genome annotation databases

EnsemblFungiiYLR234W; YLR234W; YLR234W.
GeneIDi850935.
KEGGisce:YLR234W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24939 Genomic DNA. Translation: AAA35161.1 .
U19027 Genomic DNA. Translation: AAB67406.1 .
BK006945 Genomic DNA. Translation: DAA09550.1 .
PIRi A33169. ISBYT3.
RefSeqi NP_013335.1. NM_001182121.1.

3D structure databases

ProteinModelPortali P13099.
SMRi P13099. Positions 2-633.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31503. 106 interactions.
DIPi DIP-2912N.
IntActi P13099. 3 interactions.
MINTi MINT-442624.
STRINGi 4932.YLR234W.

Proteomic databases

MaxQBi P13099.
PaxDbi P13099.
PeptideAtlasi P13099.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR234W ; YLR234W ; YLR234W .
GeneIDi 850935.
KEGGi sce:YLR234W.

Organism-specific databases

CYGDi YLR234w.
SGDi S000004224. TOP3.

Phylogenomic databases

eggNOGi COG0550.
GeneTreei ENSGT00530000063430.
HOGENOMi HOG000184377.
InParanoidi P13099.
KOi K03165.
OMAi ARFSNTE.
OrthoDBi EOG7Q8CWW.

Enzyme and pathway databases

BioCyci YEAST:G3O-32345-MONOMER.

Miscellaneous databases

NextBioi 967378.
PROi P13099.

Gene expression databases

Genevestigatori P13099.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase."
    Wallis J.W., Chrebet G., Brodsky G., Rolfe M., Rothstein R.
    Cell 58:409-419(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "An essential yeast protein, CBF5p, binds in vitro to centromeres and microtubules."
    Jiang W., Middleton K., Yoon H.-J., Fouquet C., Carbon J.
    Mol. Cell. Biol. 13:4884-4893(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 578-592; 605-615; 794-805 AND 1038-1046.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex."
    Chang M., Bellaoui M., Zhang C., Desai R., Morozov P., Delgado-Cruzata L., Rothstein R., Freyer G.A., Boone C., Brown G.W.
    EMBO J. 24:2024-2033(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  7. "Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex."
    Mullen J.R., Nallaseth F.S., Lan Y.Q., Slagle C.E., Brill S.J.
    Mol. Cell. Biol. 25:4476-4487(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiTOP3_YEAST
AccessioniPrimary (citable) accession number: P13099
Secondary accession number(s): D6VYN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3