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Protein

Enhancer of split m8 protein

Gene

E(spl)

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the control of cell fate choice by uncommitted neuroectodermal cells in the embryo (PubMed:2540957). Transcriptional repressor (PubMed:8001118). Binds DNA on N-box motifs: 5'-CACNAG-3' (PubMed:8001118). Part of the Notch signaling pathway (PubMed:22357926).3 Publications

GO - Molecular functioni

  • DNA binding transcription factor activity Source: FlyBase
  • protein heterodimerization activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • compound eye development Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • ectoderm development Source: FlyBase
  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • imaginal disc-derived wing margin morphogenesis Source: FlyBase
  • mesoderm development Source: FlyBase
  • negative regulation of gene expression Source: FlyBase
  • Notch signaling pathway Source: FlyBase
  • peripheral nervous system development Source: FlyBase
  • regulation of R8 cell spacing in compound eye Source: FlyBase
  • sensory organ precursor cell fate determination Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Enhancer of split m8 protein
Short name:
E(spl)m8
Gene namesi
Name:E(spl)
Synonyms:m8
ORF Names:CG8365
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0000591. E(spl).

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271751 – 179Enhancer of split m8 proteinAdd BLAST179

Proteomic databases

PaxDbiP13098.
PRIDEiP13098.

PTM databases

iPTMnetiP13098.

Expressioni

Developmental stagei

In larvae, detected in neuroblasts (at protein level) (PubMed:22357926). Mesectodermal expression appears shortly before the onset of gastrulation (PubMed:2540957). In imaginal disks, expression is seen primarily within presumptive proneural clusters of eye-antennal, wing and leg disks (PubMed:2540957).2 Publications

Gene expression databases

BgeeiFBgn0000591.
GenevisibleiP13098. DM.

Interactioni

Subunit structurei

Homodimer (PubMed:22357926). Heterodimers with dpn (PubMed:22357926).Transcription repression requires formation of a complex with a corepressor protein (Groucho) (PubMed:8001118).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi68061. 34 interactors.
DIPiDIP-22511N.
ELMiP13098.
IntActiP13098. 14 interactors.
MINTiP13098.
STRINGi7227.FBpp0084335.

Structurei

3D structure databases

ProteinModelPortaliP13098.
SMRiP13098.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 65bHLHPROSITE-ProRule annotationAdd BLAST56
Domaini83 – 116OrangePROSITE-ProRule annotationAdd BLAST34

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 179WRPW motif4

Domaini

The orange domain and the basic helix-loop-helix motif mediate repression of specific transcriptional activators, such as basic helix-loop-helix protein dimers.1 Publication
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.1 Publication

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
InParanoidiP13098.
KOiK09090.
OMAiGRVYKNL.
OrthoDBiEOG091G0NI7.
PhylomeDBiP13098.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR036638. HLH_DNA-bd_sf.
IPR003650. Orange_dom.
PfamiView protein in Pfam
PF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.

Sequencei

Sequence statusi: Complete.

P13098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYTTKTQIY QKVKKPMLER QRRARMNKCL DNLKTLVAEL RGDDGILRMD
60 70 80 90 100
KAEMLESAVI FMRQQKTPKK VAQEEQSLPL DSFKNGYMNA VNEVSRVMAS
110 120 130 140 150
TPGMSVDLGK SVMTHLGRVY KNLQQFHEAQ SAADFIQNSM DCSSMDKAPL
160 170
SPASSGYHSD CDSPAPSPQP MQQPLWRPW
Length:179
Mass (Da):20,303
Last modified:December 1, 2000 - v2
Checksum:i017B139DE0C328B4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165 – 167APS → PPT in CAA34554 (PubMed:2540957).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti114T → I in strain: NVIII-2, NVIII-5 and NVIII-m15. 1 Publication1
Natural varianti133A → T in strain: NVIII-m11 and NVIII-m19. 1 Publication1
Natural varianti134D → N in strain: NVIII-m19. 1 Publication1
Natural varianti141D → V in strain: NVIII-m19. 1 Publication1
Natural varianti174P → T in strain: NVIII-m12. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16553 Genomic DNA. Translation: CAA34554.1.
AY779906 Genomic DNA. Translation: AAV59051.1.
AY779907 Genomic DNA. Translation: AAV59063.1.
AY779908 Genomic DNA. Translation: AAV59075.1.
AY779909 Genomic DNA. Translation: AAV59087.1.
AY779910 Genomic DNA. Translation: AAV59099.1.
AY779911 Genomic DNA. Translation: AAV59111.1.
AY779912 Genomic DNA. Translation: AAV59123.1.
AY779913 Genomic DNA. Translation: AAV59135.1.
AY779914 Genomic DNA. Translation: AAV59147.1.
AY779915 Genomic DNA. Translation: AAV59159.1.
AY779916 Genomic DNA. Translation: AAV59171.1.
AY779917 Genomic DNA. Translation: AAV59183.1.
AY779918 Genomic DNA. Translation: AAV59195.1.
AY779919 Genomic DNA. Translation: AAV59207.1.
AY779920 Genomic DNA. Translation: AAV59219.1.
AY779921 Genomic DNA. Translation: AAV59231.1.
AE014297 Genomic DNA. Translation: AAF56555.1.
BT028829 mRNA. Translation: ABI34210.1.
BT028834 mRNA. Translation: ABI34215.2.
BT028861 mRNA. Translation: ABI34242.1.
BT028867 mRNA. Translation: ABI34248.2.
DQ138662 Genomic DNA. Translation: ABA86268.1.
PIRiS03627.
RefSeqiNP_524513.1. NM_079789.3.
UniGeneiDm.27416.

Genome annotation databases

EnsemblMetazoaiFBtr0084961; FBpp0084335; FBgn0000591.
GeneIDi43161.
KEGGidme:Dmel_CG8365.

Similar proteinsi

Entry informationi

Entry nameiESM8_DROME
AccessioniPrimary (citable) accession number: P13098
Secondary accession number(s): Q0IGQ3
, Q2XYK0, Q5S426, Q5S462, Q5S474, Q5S4H0, Q5S4I2, Q9VBI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome