Reviewed, UniProtKB/Swiss-Prot P13098 (ESM8_DROME)

Names and origin

Protein names

Recommended name:
    Enhancer of split m8 protein
      Short name=E(spl)m8

Gene names

Name:	E(spl)
Synonyms:	m8
ORF Names:	CG8365

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Participates in the control of cell fate choice by uncommitted neuroectodermal cells in the embryo. Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'.
Subunit structure	Transcription repression requires formation of a complex with a corepressor protein (Groucho). Forms homodimers By similarity.
Subcellular location	NucleusProbable.
Developmental stage	Mesectodermal expression appears shortly before the onset of gastrulation. In imaginal disks, expression is seen primarily within presumptive proneural clusters of eye-antennal, wing and leg disks.
Domain	The orange domain and the basic helix-loop-helix motif mediate repression of specific transcriptional activators, such as basic helix-loop-helix protein dimers. The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Non-traceable author statement. Source: FlyBase dendrite morphogenesis Inferred from mutant phenotype. Source: FlyBase ectoderm development Traceable author statement. Source: FlyBase epidermal growth factor receptor signaling pathway Non-traceable author statement. Source: FlyBase imaginal disc-derived wing margin morphogenesis Non-traceable author statement. Source: FlyBase mesoderm development Inferred from mutant phenotype. Source: FlyBase peripheral nervous system development Traceable author statement. Source: FlyBase regulation of R8 cell spacing in compound eye Non-traceable author statement. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory organ precursor cell fate determination Inferred from mutant phenotype. Source: FlyBase
Cellular component	nucleus Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
da	P11420	2	EBI-185388,EBI-151268
dgt2	Q9VKD6	1	EBI-185388,EBI-84322
gro	P16371	3	EBI-185388,EBI-153866

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Enhancer of split m8 protein		PRO_0000127175
Regions
	Domain	26 – 66	41	Helix-loop-helix motif
	Domain	83 – 116	34	Orange
	DNA binding	12 – 25	14	Basic motif
	Motif	176 – 179	4	WRPW motif
Experimental info
	Sequence conflict	165 – 167	3	APS → PPT in CAA34554. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13098-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 017B139DE0C328B4	FASTA	179	20,303
	10 20 30 40 50 60 MEYTTKTQIY QKVKKPMLER QRRARMNKCL DNLKTLVAEL RGDDGILRMD KAEMLESAVI 70 80 90 100 110 120 FMRQQKTPKK VAQEEQSLPL DSFKNGYMNA VNEVSRVMAS TPGMSVDLGK SVMTHLGRVY 130 140 150 160 170 KNLQQFHEAQ SAADFIQNSM DCSSMDKAPL SPASSGYHSD CDSPAPSPQP MQQPLWRPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Closely related transcripts encoded by the neurogenic gene complex enhancer of split of Drosophila melanogaster." Klaembt C., Knust E., Tietze K., Campos-Ortega J.A. EMBO J. 8:203-210(1989) [PubMed: 2540957] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins." Paroush Z., Finley R.L. Jr., Kidd T., Wainwright S.M., Ingham P.W., Brent R., Ish-Horowicz D. Cell 79:805-815(1994) [PubMed: 8001118] [Abstract] Cited for: DOMAIN WRPW MOTIF.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16553 Genomic DNA. Translation: CAA34554.1. AE014297 Genomic DNA. Translation: AAF56555.1.
PIR	S03627.
RefSeq	NP_524513.1.
UniGene	Dm.27416
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:22511N.
IntAct	P13098.
Genome annotation databases
Ensembl	CG8365. Drosophila melanogaster. [Contig view]
GeneID	43161.
KEGG	dme:Dmel_CG8365.
Organism-specific databases
FlyBase	FBgn0000591. E(spl).
Phylogenomic databases
HOGENOM	P13098.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-013506-MON.
Gene expression databases
ArrayExpress	P13098.
GermOnline	CG8365. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001092. HLH_basic. IPR003650. Orange. [Graphical view]
Pfam	PF07527. Hairy_orange. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00511. ORANGE. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. PS51054. ORANGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	832482.

Entry information

Entry name

ESM8_DROME

Accession

Primary (citable) accession number: P13098
Secondary accession number(s): Q9VBI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project