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Protein

Enhancer of split m7 protein

Gene

E(spl)m7-HLH

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the control of cell fate choice by uncommitted neuroectodermal cells in the embryo. Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'.

GO - Molecular functioni

  • DNA binding transcription factor activity Source: FlyBase
  • protein dimerization activity Source: InterPro
  • sequence-specific DNA binding Source: FlyBase
  • sequence-specific double-stranded DNA binding Source: UniProtKB
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • compound eye development Source: FlyBase
  • negative regulation of transcription by RNA polymerase II Source: FlyBase
  • nervous system development Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Enhancer of split m7 protein
Short name:
E(spl)m7
Gene namesi
Name:E(spl)m7-HLHImported
Synonyms:HLHm7Imported
ORF Names:CG8361Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0002633. E(spl)m7-HLH.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271731 – 186Enhancer of split m7 proteinAdd BLAST186

Proteomic databases

PaxDbiP13097.
PRIDEiP13097.

Expressioni

Developmental stagei

In larva, detected in the optic lobe and weakly in the neuroblasts (at protein level) (PubMed:22357926). Expressed at the time when separation of neural and epidermal precursor cells occurs (PubMed:2540957). Mesectodermal expression appears shortly before the onset of gastrulation (PubMed:2540957). In imaginal disks, expression is seen primarily within presumptive proneural clusters of eye-antennal, wing and leg disks (PubMed:2540957).2 Publications

Gene expression databases

BgeeiFBgn0002633.
GenevisibleiP13097. DM.

Interactioni

Subunit structurei

Transcription repression requires formation of a complex with a corepressor protein (Groucho). Forms homodimers.By similarity

GO - Molecular functioni

  • protein dimerization activity Source: InterPro
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi68060. 33 interactors.
DIPiDIP-626N.
ELMiP13097.
IntActiP13097. 29 interactors.
MINTiP13097.
STRINGi7227.FBpp0084334.

Structurei

3D structure databases

ProteinModelPortaliP13097.
SMRiP13097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 68bHLHPROSITE-ProRule annotationAdd BLAST56
Domaini83 – 116OrangePROSITE-ProRule annotationAdd BLAST34

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi183 – 186WRPW motif4

Domaini

The orange domain and the basic helix-loop-helix motif mediate repression of specific transcriptional activators, such as basic helix-loop-helix protein dimers.1 Publication
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.1 Publication

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00700000104168.
InParanoidiP13097.
KOiK09090.
OMAiGQVWRPW.
OrthoDBiEOG091G0P1S.
PhylomeDBiP13097.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR036638. HLH_DNA-bd_sf.
IPR003650. Orange_dom.
PfamiView protein in Pfam
PF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.

Sequencei

Sequence statusi: Complete.

P13097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKYEMSKT YQYRKVMKPL LERKRRARIN KCLDELKDLM AECVAQTGDA
60 70 80 90 100
KFEKADILEV TVQHLRKLKE SKKHVPANPE QSFRAGYIRA ANEVSRALAS
110 120 130 140 150
LPRVDVAFGT TLMTHLGMRL NQLEQPMEQP QAVNTPLSIV CGSSSSSSTY
160 170 180
SSASSCSSIS PVSSGYASDN ESLLQISSPG QVWRPW
Length:186
Mass (Da):20,687
Last modified:January 1, 1990 - v1
Checksum:i9330530963CDCE15
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti155 – 156Missing in strain: NVIII-9 and NVIII-46. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16553 Genomic DNA. Translation: CAA34553.1.
AY779906 Genomic DNA. Translation: AAV59050.1.
AY779907 Genomic DNA. Translation: AAV59062.1.
AY779908 Genomic DNA. Translation: AAV59074.1.
AY779909 Genomic DNA. Translation: AAV59086.1.
AY779910 Genomic DNA. Translation: AAV59098.1.
AY779911 Genomic DNA. Translation: AAV59110.1.
AY779912 Genomic DNA. Translation: AAV59122.1.
AY779913 Genomic DNA. Translation: AAV59134.1.
AY779914 Genomic DNA. Translation: AAV59146.1.
AY779915 Genomic DNA. Translation: AAV59158.1.
AY779916 Genomic DNA. Translation: AAV59170.1.
AY779917 Genomic DNA. Translation: AAV59182.1.
AY779918 Genomic DNA. Translation: AAV59194.1.
AY779919 Genomic DNA. Translation: AAV59206.1.
AY779920 Genomic DNA. Translation: AAV59218.1.
AY779921 Genomic DNA. Translation: AAV59230.1.
AE014297 Genomic DNA. Translation: AAF56554.1.
BT022121 mRNA. Translation: AAY51516.1.
PIRiS03626.
RefSeqiNP_536753.1. NM_080505.3.
UniGeneiDm.24100.

Genome annotation databases

EnsemblMetazoaiFBtr0084960; FBpp0084334; FBgn0002633.
GeneIDi43160.
KEGGidme:Dmel_CG8361.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiESM7_DROME
AccessioniPrimary (citable) accession number: P13097
Secondary accession number(s): Q5S487, Q5S4J5, Q9VBI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: March 28, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome