Aminotriazole resistance protein - Saccharomyces cerevisiae (Baker's yeast)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P13090 (ATR1_YEAST)

Last modified January 19, 2010. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
Aminotriazole resistance protein

Gene names

Name:	ATR1
Synonyms:	SNQ1
Ordered Locus Names:	YML116W
ORF Names:	YM8339.03

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Putative component of the machinery responsible for pumping aminotriazole (and possibly other toxic compounds) out of the cell. Probable ATP-dependent export permease. Appears to confer resistance only to aminotriazole.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	Deletion of the C-terminal 34 residues abolishes ATR1 activity, while deletion of the C-terminal 23 residues have a minor effect.
Miscellaneous	Present with 1720 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the major facilitator superfamily.

Hide | Top

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	boron transport Inferred from mutant phenotype. Source: SGD multidrug transport Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.4 Inferred from direct assay. Source: SGD vacuole Inferred from direct assay. Source: SGD
Molecular function	boron transporter activity Inferred from mutant phenotype. Source: SGD multidrug efflux pump activity Ref.4 Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-3328,EBI-24597

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 542

542

Aminotriazole resistance protein

PRO_0000173367

Regions

Topological domain

1 – 108

108

Cytoplasmic Potential

Transmembrane

109 – 129

Potential

Topological domain

130 – 136

Extracellular Potential

Transmembrane

137 – 157

Potential

Topological domain

158 – 172

Cytoplasmic Potential

Transmembrane

173 – 193

Potential

Topological domain

194 – 198

Extracellular Potential

Transmembrane

199 – 219

Potential

Topological domain

220 – 231

Cytoplasmic Potential

Transmembrane

232 – 252

Potential

Topological domain

253 – 262

Extracellular Potential

Transmembrane

263 – 283

Potential

Topological domain

284 – 295

Cytoplasmic Potential

Transmembrane

296 – 316

Potential

Topological domain

317 – 333

Extracellular Potential

Transmembrane

334 – 354

Potential

Topological domain

355 – 371

Cytoplasmic Potential

Transmembrane

372 – 392

Potential

Topological domain

393 – 399

Extracellular Potential

Transmembrane

400 – 420

Potential

Topological domain

421 – 429

Cytoplasmic Potential

Transmembrane

430 – 450

Potential

Topological domain

451 – 505

Extracellular Potential

Transmembrane

506 – 526

Potential

Topological domain

527 – 542

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphothreonine Ref.7

Modified residue

Phosphoserine Ref.7

Glycosylation

394

N-linked (GlcNAc...) Potential

Glycosylation

471

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

531 – 542

ARAAA…DCTVA → QELLQNTIALWLSGKRY in AAA34449. Ref.1

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P13090-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 5DDA45815068CD41
Show »

FASTA

542

59,983

        10         20         30         40         50         60 
MGNQSLVVLT ESKGEYENET ELPVKKSSRD NNIGESLTAT AFTQSEDEMV DSNQKWQNPN 

        70         80         90        100        110        120 
YFKYAWQEYL FIFTCMISQL LNQAGTTQTL SIMNILSDSF GSEGNSKSWL MASFPLVSGS 

       130        140        150        160        170        180 
FILISGRLGD IYGLKKMLLV GYVLVIIWSL ICGITKYSGS DTFFIISRAF QGLGIAFVLP 

       190        200        210        220        230        240 
NVLGIIGNIY VGGTFRKNIV ISFVGAMAPI GATLGCLFAG LIGTEDPKQW PWAFYAYSIA 

       250        260        270        280        290        300 
AFINFVLSIY AIPSTIPTNI HHFSMDWIGS VLGVIGLILL NFVWNQAPIS GWNQAYIIVI 

       310        320        330        340        350        360 
LIISVIFLVV FIIYEIRFAK TPLLPRAVIK DRHMIQIMLA LFFGWGSFGI FTFYYFQFQL 

       370        380        390        400        410        420 
NIRQYTALWA GGTYFMFLIW GIIAALLVGF TIKNVSPSVF LFFSMVAFNV GSIMASVTPV 

       430        440        450        460        470        480 
HETYFRTQLG TMIILSFGMD LSFPASSIIF SDNLPMEYQG MAGSLVNTVV NYSMSLCLGM 

       490        500        510        520        530        540 
GATVETQVNS DGKHLLKGYR GAQYLGIGLA SLACMISGLY MVESFIKGRR ARAAAEYDCT 


VA

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance." Kanazawa S., Driscoll M., Struhl K. Mol. Cell. Biol. 8:664-673(1988) [PubMed: 3280970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Allelism of SNQ1 and ATR1, genes of the yeast Saccharomyces cerevisiae required for controlling sensitivity to 4-nitroquinoline-N-oxide and aminotriazole." Goempel-Klein P., Brendel M. Curr. Genet. 18:93-96(1990) [PubMed: 2123132] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-12; THR-43 AND SER-45, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20319 Genomic DNA. Translation: AAA34449.1. Z49210 Genomic DNA. Translation: CAA89102.1. AY723850 Genomic DNA. Translation: AAU09767.1.
PIR	S53956.
RefSeq	NP_013591.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3953N.
IntAct	P13090. 4 interactions.
STRING	P13090.
Protein family/group databases
TCDB	2.A.1.3.1. major facilitator superfamily (MFS).
Genome annotation databases
Ensembl	YML116W; YML116W; YML116W; Saccharomyces cerevisiae. [Genome view]
GeneID	854924.
KEGG	sce:YML116W.
NMPDR	fig\|4932.3.peg.4627.
Organism-specific databases
CYGD	YML116w.
SGD	S000004584. ATR1.
Phylogenomic databases
eggNOG	fuNOG05585.
HOGENOM	HBG325775.
OMA	FFIICRA.
OrthoDB	EOG983FMM.
PhylomeDB	P13090.
Gene expression databases
ArrayExpress	P13090.
Genevestigator	P13090.
GermOnline	YML116W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011701. MFS_1. IPR016196. MFS_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977941.

Hide | Top

Entry information

Entry name

ATR1_YEAST

Accession

Primary (citable) accession number: P13090

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents