Aminotriazole resistance protein - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P13090 (ATR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aminotriazole resistance protein

Gene names

Name:	ATR1
Synonyms:	SNQ1
Ordered Locus Names:	YML116W
ORF Names:	YM8339.03

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative component of the machinery responsible for pumping aminotriazole (and possibly other toxic compounds) out of the cell. Probable ATP-dependent export permease. Appears to confer resistance only to aminotriazole.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	Deletion of the C-terminal 34 residues abolishes ATR1 activity, while deletion of the C-terminal 23 residues have a minor effect.
Miscellaneous	Present with 1720 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the major facilitator superfamily.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: SGD vacuole Inferred from direct assay. Source: SGD
Molecular function	borate transmembrane transporter activity Inferred from mutant phenotype. Source: SGD drug transmembrane transporter activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YHR080C	P38800	1	EBI-3328,EBI-24597

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 542

542

Aminotriazole resistance protein

PRO_0000173367

Regions

Topological domain

1 – 108

108

Cytoplasmic Potential

Transmembrane

109 – 129

Helical; Potential

Topological domain

130 – 136

Extracellular Potential

Transmembrane

137 – 157

Helical; Potential

Topological domain

158 – 172

Cytoplasmic Potential

Transmembrane

173 – 193

Helical; Potential

Topological domain

194 – 198

Extracellular Potential

Transmembrane

199 – 219

Helical; Potential

Topological domain

220 – 231

Cytoplasmic Potential

Transmembrane

232 – 252

Helical; Potential

Topological domain

253 – 262

Extracellular Potential

Transmembrane

263 – 283

Helical; Potential

Topological domain

284 – 295

Cytoplasmic Potential

Transmembrane

296 – 316

Helical; Potential

Topological domain

317 – 333

Extracellular Potential

Transmembrane

334 – 354

Helical; Potential

Topological domain

355 – 371

Cytoplasmic Potential

Transmembrane

372 – 392

Helical; Potential

Topological domain

393 – 399

Extracellular Potential

Transmembrane

400 – 420

Helical; Potential

Topological domain

421 – 429

Cytoplasmic Potential

Transmembrane

430 – 450

Helical; Potential

Topological domain

451 – 505

Extracellular Potential

Transmembrane

506 – 526

Helical; Potential

Topological domain

527 – 542

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphothreonine Ref.8

Modified residue

Phosphoserine Ref.8

Glycosylation

394

N-linked (GlcNAc...) Potential

Glycosylation

471

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

531 – 542

ARAAA…DCTVA → QELLQNTIALWLSGKRY in AAA34449. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P13090 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 5DDA45815068CD41
Show »

FASTA

542

59,983

        10         20         30         40         50         60 
MGNQSLVVLT ESKGEYENET ELPVKKSSRD NNIGESLTAT AFTQSEDEMV DSNQKWQNPN 

        70         80         90        100        110        120 
YFKYAWQEYL FIFTCMISQL LNQAGTTQTL SIMNILSDSF GSEGNSKSWL MASFPLVSGS 

       130        140        150        160        170        180 
FILISGRLGD IYGLKKMLLV GYVLVIIWSL ICGITKYSGS DTFFIISRAF QGLGIAFVLP 

       190        200        210        220        230        240 
NVLGIIGNIY VGGTFRKNIV ISFVGAMAPI GATLGCLFAG LIGTEDPKQW PWAFYAYSIA 

       250        260        270        280        290        300 
AFINFVLSIY AIPSTIPTNI HHFSMDWIGS VLGVIGLILL NFVWNQAPIS GWNQAYIIVI 

       310        320        330        340        350        360 
LIISVIFLVV FIIYEIRFAK TPLLPRAVIK DRHMIQIMLA LFFGWGSFGI FTFYYFQFQL 

       370        380        390        400        410        420 
NIRQYTALWA GGTYFMFLIW GIIAALLVGF TIKNVSPSVF LFFSMVAFNV GSIMASVTPV 

       430        440        450        460        470        480 
HETYFRTQLG TMIILSFGMD LSFPASSIIF SDNLPMEYQG MAGSLVNTVV NYSMSLCLGM 

       490        500        510        520        530        540 
GATVETQVNS DGKHLLKGYR GAQYLGIGLA SLACMISGLY MVESFIKGRR ARAAAEYDCT 


VA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance." Kanazawa S., Driscoll M., Struhl K. Mol. Cell. Biol. 8:664-673(1988) [PubMed: 3280970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Allelism of SNQ1 and ATR1, genes of the yeast Saccharomyces cerevisiae required for controlling sensitivity to 4-nitroquinoline-N-oxide and aminotriazole." Goempel-Klein P., Brendel M. Curr. Genet. 18:93-96(1990) [PubMed: 2123132] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-12; THR-43 AND SER-45, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20319 Genomic DNA. Translation: AAA34449.1. Z49210 Genomic DNA. Translation: CAA89102.1. AY723850 Genomic DNA. Translation: AAU09767.1. BK006946 Genomic DNA. Translation: DAA09782.1.
PIR	S53956.
RefSeq	NP_013591.1. NM_001182478.1.
3D structure databases
ProteinModelPortal	P13090.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3953N.
IntAct	P13090. 2 interactions.
MINT	MINT-548217.
STRING	P13090.
Protein family/group databases
TCDB	2.A.1.3.1. major facilitator superfamily (MFS).
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML116W; YML116W; YML116W.
GeneID	854924.
KEGG	sce:YML116W.
NMPDR	fig\|4932.3.peg.4627.
Organism-specific databases
CYGD	YML116w.
SGD	S000004584. ATR1.
Phylogenomic databases
eggNOG	fuNOG05585.
GeneTree	EFGT00050000000293.
HOGENOM	HBG325775.
OMA	FWILAIV.
OrthoDB	EOG48KVKW.
Gene expression databases
ArrayExpress	P13090.
Genevestigator	P13090.
GermOnline	YML116W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
KO	K08165.
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977941.

Entry information

Entry name

ATR1_YEAST

Accession

Primary (citable) accession number: P13090
Secondary accession number(s): D6W0G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents