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Protein

Aminotriazole resistance protein

Gene

ATR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative component of the machinery responsible for pumping aminotriazole (and possibly other toxic compounds) out of the cell. Probable ATP-dependent export permease. Appears to confer resistance only to aminotriazole.

GO - Molecular functioni

  • borate efflux transmembrane transporter activity Source: SGD
  • drug transmembrane transporter activity Source: SGD

GO - Biological processi

  • borate transmembrane transport Source: GOC
  • borate transport Source: SGD
  • drug transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32697-MONOMER.

Protein family/group databases

TCDBi2.A.1.3.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Aminotriazole resistance protein
Gene namesi
Name:ATR1
Synonyms:SNQ1
Ordered Locus Names:YML116W
ORF Names:YM8339.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML116W.
SGDiS000004584. ATR1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 108108CytoplasmicSequence analysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence analysisAdd
BLAST
Topological domaini130 – 1367ExtracellularSequence analysis
Transmembranei137 – 15721HelicalSequence analysisAdd
BLAST
Topological domaini158 – 17215CytoplasmicSequence analysisAdd
BLAST
Transmembranei173 – 19321HelicalSequence analysisAdd
BLAST
Topological domaini194 – 1985ExtracellularSequence analysis
Transmembranei199 – 21921HelicalSequence analysisAdd
BLAST
Topological domaini220 – 23112CytoplasmicSequence analysisAdd
BLAST
Transmembranei232 – 25221HelicalSequence analysisAdd
BLAST
Topological domaini253 – 26210ExtracellularSequence analysis
Transmembranei263 – 28321HelicalSequence analysisAdd
BLAST
Topological domaini284 – 29512CytoplasmicSequence analysisAdd
BLAST
Transmembranei296 – 31621HelicalSequence analysisAdd
BLAST
Topological domaini317 – 33317ExtracellularSequence analysisAdd
BLAST
Transmembranei334 – 35421HelicalSequence analysisAdd
BLAST
Topological domaini355 – 37117CytoplasmicSequence analysisAdd
BLAST
Transmembranei372 – 39221HelicalSequence analysisAdd
BLAST
Topological domaini393 – 3997ExtracellularSequence analysis
Transmembranei400 – 42021HelicalSequence analysisAdd
BLAST
Topological domaini421 – 4299CytoplasmicSequence analysis
Transmembranei430 – 45021HelicalSequence analysisAdd
BLAST
Topological domaini451 – 50555ExtracellularSequence analysisAdd
BLAST
Transmembranei506 – 52621HelicalSequence analysisAdd
BLAST
Topological domaini527 – 54216CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
  • vacuole Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Aminotriazole resistance proteinPRO_0000173367Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi394 – 3941N-linked (GlcNAc...)Sequence analysis
Glycosylationi471 – 4711N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP13090.

PTM databases

iPTMnetiP13090.

Interactioni

Protein-protein interaction databases

BioGridi35088. 6 interactions.
DIPiDIP-3953N.
IntActiP13090. 2 interactions.
MINTiMINT-548217.

Structurei

3D structure databases

ProteinModelPortaliP13090.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Deletion of the C-terminal 34 residues abolishes ATR1 activity, while deletion of the C-terminal 23 residues have a minor effect.

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000001190.
HOGENOMiHOG000174951.
InParanoidiP13090.
KOiK08165.
OMAiDTHEPAR.
OrthoDBiEOG70CRGB.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNQSLVVLT ESKGEYENET ELPVKKSSRD NNIGESLTAT AFTQSEDEMV
60 70 80 90 100
DSNQKWQNPN YFKYAWQEYL FIFTCMISQL LNQAGTTQTL SIMNILSDSF
110 120 130 140 150
GSEGNSKSWL MASFPLVSGS FILISGRLGD IYGLKKMLLV GYVLVIIWSL
160 170 180 190 200
ICGITKYSGS DTFFIISRAF QGLGIAFVLP NVLGIIGNIY VGGTFRKNIV
210 220 230 240 250
ISFVGAMAPI GATLGCLFAG LIGTEDPKQW PWAFYAYSIA AFINFVLSIY
260 270 280 290 300
AIPSTIPTNI HHFSMDWIGS VLGVIGLILL NFVWNQAPIS GWNQAYIIVI
310 320 330 340 350
LIISVIFLVV FIIYEIRFAK TPLLPRAVIK DRHMIQIMLA LFFGWGSFGI
360 370 380 390 400
FTFYYFQFQL NIRQYTALWA GGTYFMFLIW GIIAALLVGF TIKNVSPSVF
410 420 430 440 450
LFFSMVAFNV GSIMASVTPV HETYFRTQLG TMIILSFGMD LSFPASSIIF
460 470 480 490 500
SDNLPMEYQG MAGSLVNTVV NYSMSLCLGM GATVETQVNS DGKHLLKGYR
510 520 530 540
GAQYLGIGLA SLACMISGLY MVESFIKGRR ARAAAEYDCT VA
Length:542
Mass (Da):59,983
Last modified:October 1, 1996 - v2
Checksum:i5DDA45815068CD41
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti531 – 54212ARAAA…DCTVA → QELLQNTIALWLSGKRY in AAA34449 (PubMed:3280970).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20319 Genomic DNA. Translation: AAA34449.1.
Z49210 Genomic DNA. Translation: CAA89102.1.
AY723850 Genomic DNA. Translation: AAU09767.1.
BK006946 Genomic DNA. Translation: DAA09782.1.
PIRiS53956.
RefSeqiNP_013591.1. NM_001182478.1.

Genome annotation databases

EnsemblFungiiYML116W; YML116W; YML116W.
GeneIDi854924.
KEGGisce:YML116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20319 Genomic DNA. Translation: AAA34449.1.
Z49210 Genomic DNA. Translation: CAA89102.1.
AY723850 Genomic DNA. Translation: AAU09767.1.
BK006946 Genomic DNA. Translation: DAA09782.1.
PIRiS53956.
RefSeqiNP_013591.1. NM_001182478.1.

3D structure databases

ProteinModelPortaliP13090.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35088. 6 interactions.
DIPiDIP-3953N.
IntActiP13090. 2 interactions.
MINTiMINT-548217.

Protein family/group databases

TCDBi2.A.1.3.1. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP13090.

Proteomic databases

MaxQBiP13090.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML116W; YML116W; YML116W.
GeneIDi854924.
KEGGisce:YML116W.

Organism-specific databases

EuPathDBiFungiDB:YML116W.
SGDiS000004584. ATR1.

Phylogenomic databases

GeneTreeiENSGT00390000001190.
HOGENOMiHOG000174951.
InParanoidiP13090.
KOiK08165.
OMAiDTHEPAR.
OrthoDBiEOG70CRGB.

Enzyme and pathway databases

BioCyciYEAST:G3O-32697-MONOMER.

Miscellaneous databases

NextBioi977941.
PROiP13090.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance."
    Kanazawa S., Driscoll M., Struhl K.
    Mol. Cell. Biol. 8:664-673(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Allelism of SNQ1 and ATR1, genes of the yeast Saccharomyces cerevisiae required for controlling sensitivity to 4-nitroquinoline-N-oxide and aminotriazole."
    Goempel-Klein P., Brendel M.
    Curr. Genet. 18:93-96(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiATR1_YEAST
AccessioniPrimary (citable) accession number: P13090
Secondary accession number(s): D6W0G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.