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P13087

- MIRA_SYNDU

UniProt

P13087 - MIRA_SYNDU

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Protein

Miraculin

Gene
N/A
Organism
Synsepalum dulcificum (Miracle fruit) (Richadella dulcifica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Miraculin has the property of modifying a sour taste into a sweet taste. This alteration of taste perception persists for many minutes.

GO - Molecular functioni

  1. endopeptidase inhibitor activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Taste-modifying protein

Protein family/group databases

MEROPSiI03.030.

Names & Taxonomyi

Protein namesi
Recommended name:
Miraculin
Short name:
MIR
OrganismiSynsepalum dulcificum (Miracle fruit) (Richadella dulcifica)
Taxonomic identifieri3743 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesSapotaceaeSynsepalum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29292 PublicationsAdd
BLAST
Chaini30 – 220191MiraculinPRO_0000016935Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)1 PublicationCAR_000132
Disulfide bondi76 ↔ 1211 Publication
Disulfide bondi167 – 167Interchain1 Publication
Disulfide bondi177 ↔ 1881 Publication
Disulfide bondi181 ↔ 1841 Publication
Glycosylationi215 – 2151N-linked (GlcNAc...)1 PublicationCAR_000133

Post-translational modificationi

Glycosylated; contains as much as 13,9% of sugars (glucosamine, mannose, galactose, xylose, and fucose).1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

PTM databases

UniCarbKBiP13087.

Expressioni

Tissue specificityi

Expressed in fruit pulp after pollination. Not expressed in seeds, stems or leaves.1 Publication

Interactioni

Subunit structurei

Homotetramer; dimer of homodimer.

Structurei

3D structure databases

ProteinModelPortaliP13087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR011065. Kunitz_inhibitor_ST1-like.
IPR002160. Prot_inh_Kunz-lg.
[Graphical view]
PfamiPF00197. Kunitz_legume. 1 hit.
[Graphical view]
PRINTSiPR00291. KUNITZINHBTR.
SMARTiSM00452. STI. 1 hit.
[Graphical view]
SUPFAMiSSF50386. SSF50386. 1 hit.
PROSITEiPS00283. SOYBEAN_KUNITZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13087-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKELTMLSLS FFFVSALLAA AANPLLSAAD SAPNPVLDID GEKLRTGTNY
60 70 80 90 100
YIVPVLRDHG GGLTVSATTP NGTFVCPPRV VQTRKEVDHD RPLAFFPENP
110 120 130 140 150
KEDVVRVSTD LNINFSAFMP CRWTSSTVWR LDKYDESTGQ YFVTIGGVKG
160 170 180 190 200
NPGPETISSW FKIEEFCGSG FYKLVFCPTV CGSCKVKCGD VGIYIDQKGR
210 220
RRLALSDKPF AFEFNKTVYF
Length:220
Mass (Da):24,367
Last modified:July 15, 1999 - v3
Checksum:iBD7E70CE1AAEE520
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291W → S AA sequence (PubMed:2708331)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38598 mRNA. Translation: BAA07603.1.
PIRiJC4232. A33872.

Cross-referencesi

Web resourcesi

Protein Spotlight

The sweet side of life - Issue 17 of December 2001

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38598 mRNA. Translation: BAA07603.1 .
PIRi JC4232. A33872.

3D structure databases

ProteinModelPortali P13087.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi I03.030.

PTM databases

UniCarbKBi P13087.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011065. Kunitz_inhibitor_ST1-like.
IPR002160. Prot_inh_Kunz-lg.
[Graphical view ]
Pfami PF00197. Kunitz_legume. 1 hit.
[Graphical view ]
PRINTSi PR00291. KUNITZINHBTR.
SMARTi SM00452. STI. 1 hit.
[Graphical view ]
SUPFAMi SSF50386. SSF50386. 1 hit.
PROSITEi PS00283. SOYBEAN_KUNITZ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of a cDNA encoding a taste-modifying protein, miraculin."
    Masuda Y., Nirasawa S., Nakaya K., Kurihara Y.
    Gene 161:175-177(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Fruit.
  2. "Complete amino acid sequence and structure characterization of the taste-modifying protein, miraculin."
    Theerasilp S., Hitotsuya H., Nakajo S., Nakaja K., Nakamura Y., Kurihara Y.
    J. Biol. Chem. 264:6655-6659(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-220.
  3. "Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit."
    Theerasilp S., Kurihara Y.
    J. Biol. Chem. 263:11536-11539(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-50.
  4. "Determination of disulfide array and subunit structure of taste-modifying protein, miraculin."
    Igeta H., Tamura Y., Nakaya K., Nakmura Y., Kurihara Y.
    Biochim. Biophys. Acta 1079:303-307(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  5. "Structural study of asparagine-linked oligosaccharide moiety of taste-modifying protein, miraculin."
    Takahashi N., Hitotsuya H., Hanzawa H., Arata Y., Kurihara Y.
    J. Biol. Chem. 265:7793-7798(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-71 AND ASN-215.

Entry informationi

Entry nameiMIRA_SYNDU
AccessioniPrimary (citable) accession number: P13087
Secondary accession number(s): Q41153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3