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P13087

- MIRA_SYNDU

UniProt

P13087 - MIRA_SYNDU

Protein

Miraculin

Gene
N/A
Organism
Synsepalum dulcificum (Miracle fruit) (Richadella dulcifica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 3 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Miraculin has the property of modifying a sour taste into a sweet taste. This alteration of taste perception persists for many minutes.

    GO - Molecular functioni

    1. endopeptidase inhibitor activity Source: InterPro

    Keywords - Molecular functioni

    Taste-modifying protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Miraculin
    Short name:
    MIR
    OrganismiSynsepalum dulcificum (Miracle fruit) (Richadella dulcifica)
    Taxonomic identifieri3743 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesSapotaceaeSynsepalum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 29292 PublicationsAdd
    BLAST
    Chaini30 – 220191MiraculinPRO_0000016935Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)1 PublicationCAR_000132
    Disulfide bondi76 ↔ 1211 Publication
    Disulfide bondi167 – 167Interchain1 Publication
    Disulfide bondi177 ↔ 1881 Publication
    Disulfide bondi181 ↔ 1841 Publication
    Glycosylationi215 – 2151N-linked (GlcNAc...)1 PublicationCAR_000133

    Post-translational modificationi

    Glycosylated; contains as much as 13,9% of sugars (glucosamine, mannose, galactose, xylose, and fucose).1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    PTM databases

    UniCarbKBiP13087.

    Expressioni

    Tissue specificityi

    Expressed in fruit pulp after pollination. Not expressed in seeds, stems or leaves.1 Publication

    Interactioni

    Subunit structurei

    Homotetramer; dimer of homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP13087.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR011065. Kunitz_inhibitor_ST1-like.
    IPR002160. Prot_inh_Kunz-lg.
    [Graphical view]
    PfamiPF00197. Kunitz_legume. 1 hit.
    [Graphical view]
    PRINTSiPR00291. KUNITZINHBTR.
    SMARTiSM00452. STI. 1 hit.
    [Graphical view]
    SUPFAMiSSF50386. SSF50386. 1 hit.
    PROSITEiPS00283. SOYBEAN_KUNITZ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13087-1 [UniParc]FASTAAdd to Basket

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    MKELTMLSLS FFFVSALLAA AANPLLSAAD SAPNPVLDID GEKLRTGTNY    50
    YIVPVLRDHG GGLTVSATTP NGTFVCPPRV VQTRKEVDHD RPLAFFPENP 100
    KEDVVRVSTD LNINFSAFMP CRWTSSTVWR LDKYDESTGQ YFVTIGGVKG 150
    NPGPETISSW FKIEEFCGSG FYKLVFCPTV CGSCKVKCGD VGIYIDQKGR 200
    RRLALSDKPF AFEFNKTVYF 220
    Length:220
    Mass (Da):24,367
    Last modified:July 15, 1999 - v3
    Checksum:iBD7E70CE1AAEE520
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti129 – 1291W → S AA sequence (PubMed:2708331)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38598 mRNA. Translation: BAA07603.1.
    PIRiJC4232. A33872.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The sweet side of life - Issue 17 of December 2001

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38598 mRNA. Translation: BAA07603.1 .
    PIRi JC4232. A33872.

    3D structure databases

    ProteinModelPortali P13087.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    UniCarbKBi P13087.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011065. Kunitz_inhibitor_ST1-like.
    IPR002160. Prot_inh_Kunz-lg.
    [Graphical view ]
    Pfami PF00197. Kunitz_legume. 1 hit.
    [Graphical view ]
    PRINTSi PR00291. KUNITZINHBTR.
    SMARTi SM00452. STI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50386. SSF50386. 1 hit.
    PROSITEi PS00283. SOYBEAN_KUNITZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a cDNA encoding a taste-modifying protein, miraculin."
      Masuda Y., Nirasawa S., Nakaya K., Kurihara Y.
      Gene 161:175-177(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Fruit.
    2. "Complete amino acid sequence and structure characterization of the taste-modifying protein, miraculin."
      Theerasilp S., Hitotsuya H., Nakajo S., Nakaja K., Nakamura Y., Kurihara Y.
      J. Biol. Chem. 264:6655-6659(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 30-220.
    3. "Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit."
      Theerasilp S., Kurihara Y.
      J. Biol. Chem. 263:11536-11539(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 30-50.
    4. "Determination of disulfide array and subunit structure of taste-modifying protein, miraculin."
      Igeta H., Tamura Y., Nakaya K., Nakmura Y., Kurihara Y.
      Biochim. Biophys. Acta 1079:303-307(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.
    5. "Structural study of asparagine-linked oligosaccharide moiety of taste-modifying protein, miraculin."
      Takahashi N., Hitotsuya H., Hanzawa H., Arata Y., Kurihara Y.
      J. Biol. Chem. 265:7793-7798(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-71 AND ASN-215.

    Entry informationi

    Entry nameiMIRA_SYNDU
    AccessioniPrimary (citable) accession number: P13087
    Secondary accession number(s): Q41153
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 86 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3