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P13087

- MIRA_SYNDU

UniProt

P13087 - MIRA_SYNDU

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Protein
Miraculin
Gene
N/A
Organism
Synsepalum dulcificum (Miracle fruit) (Richadella dulcifica)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Miraculin has the property of modifying a sour taste into a sweet taste. This alteration of taste perception persists for many minutes.

GO - Molecular functioni

  1. endopeptidase inhibitor activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Taste-modifying protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Miraculin
    Short name:
    MIR
    OrganismiSynsepalum dulcificum (Miracle fruit) (Richadella dulcifica)
    Taxonomic identifieri3743 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesSapotaceaeSynsepalum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 29292 Publications
    Add
    BLAST
    Chaini30 – 220191Miraculin
    PRO_0000016935Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)1 Publication
    CAR_000132
    Disulfide bondi76 ↔ 1211 Publication
    Disulfide bondi167 – 167Interchain1 Publication
    Disulfide bondi177 ↔ 1881 Publication
    Disulfide bondi181 ↔ 1841 Publication
    Glycosylationi215 – 2151N-linked (GlcNAc...)1 Publication
    CAR_000133

    Post-translational modificationi

    Glycosylated; contains as much as 13,9% of sugars (glucosamine, mannose, galactose, xylose, and fucose).1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    PTM databases

    UniCarbKBiP13087.

    Expressioni

    Tissue specificityi

    Expressed in fruit pulp after pollination. Not expressed in seeds, stems or leaves.1 Publication

    Interactioni

    Subunit structurei

    Homotetramer; dimer of homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP13087.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR011065. Kunitz_inhibitor_ST1-like.
    IPR002160. Prot_inh_Kunz-lg.
    [Graphical view]
    PfamiPF00197. Kunitz_legume. 1 hit.
    [Graphical view]
    PRINTSiPR00291. KUNITZINHBTR.
    SMARTiSM00452. STI. 1 hit.
    [Graphical view]
    SUPFAMiSSF50386. SSF50386. 1 hit.
    PROSITEiPS00283. SOYBEAN_KUNITZ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13087-1 [UniParc]FASTAAdd to Basket

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    MKELTMLSLS FFFVSALLAA AANPLLSAAD SAPNPVLDID GEKLRTGTNY    50
    YIVPVLRDHG GGLTVSATTP NGTFVCPPRV VQTRKEVDHD RPLAFFPENP 100
    KEDVVRVSTD LNINFSAFMP CRWTSSTVWR LDKYDESTGQ YFVTIGGVKG 150
    NPGPETISSW FKIEEFCGSG FYKLVFCPTV CGSCKVKCGD VGIYIDQKGR 200
    RRLALSDKPF AFEFNKTVYF 220
    Length:220
    Mass (Da):24,367
    Last modified:July 15, 1999 - v3
    Checksum:iBD7E70CE1AAEE520
    GO

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti129 – 1291W → S AA sequence 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38598 mRNA. Translation: BAA07603.1.
    PIRiJC4232. A33872.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The sweet side of life - Issue 17 of December 2001

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38598 mRNA. Translation: BAA07603.1 .
    PIRi JC4232. A33872.

    3D structure databases

    ProteinModelPortali P13087.
    ModBasei Search...

    PTM databases

    UniCarbKBi P13087.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011065. Kunitz_inhibitor_ST1-like.
    IPR002160. Prot_inh_Kunz-lg.
    [Graphical view ]
    Pfami PF00197. Kunitz_legume. 1 hit.
    [Graphical view ]
    PRINTSi PR00291. KUNITZINHBTR.
    SMARTi SM00452. STI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50386. SSF50386. 1 hit.
    PROSITEi PS00283. SOYBEAN_KUNITZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a cDNA encoding a taste-modifying protein, miraculin."
      Masuda Y., Nirasawa S., Nakaya K., Kurihara Y.
      Gene 161:175-177(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Fruit.
    2. "Complete amino acid sequence and structure characterization of the taste-modifying protein, miraculin."
      Theerasilp S., Hitotsuya H., Nakajo S., Nakaja K., Nakamura Y., Kurihara Y.
      J. Biol. Chem. 264:6655-6659(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 30-220.
    3. "Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit."
      Theerasilp S., Kurihara Y.
      J. Biol. Chem. 263:11536-11539(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 30-50.
    4. "Determination of disulfide array and subunit structure of taste-modifying protein, miraculin."
      Igeta H., Tamura Y., Nakaya K., Nakmura Y., Kurihara Y.
      Biochim. Biophys. Acta 1079:303-307(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.
    5. "Structural study of asparagine-linked oligosaccharide moiety of taste-modifying protein, miraculin."
      Takahashi N., Hitotsuya H., Hanzawa H., Arata Y., Kurihara Y.
      J. Biol. Chem. 265:7793-7798(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-71 AND ASN-215.

    Entry informationi

    Entry nameiMIRA_SYNDU
    AccessioniPrimary (citable) accession number: P13087
    Secondary accession number(s): Q41153
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: July 15, 1999
    Last modified: April 16, 2014
    This is version 85 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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