P13086 (SUCA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
EC=6.2.1.4
EC=6.2.1.5

Alternative name(s):
Succinyl-CoA synthetase subunit alpha
Short name=SCS-alpha

Gene names

Name:

Suclg1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. The nature of the beta subunit determines the nucleotide specificity By similarity.
Catalytic activity	GTP + succinate + CoA = GDP + phosphate + succinyl-CoA. ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the succinate/malate CoA ligase alpha subunit family.
Sequence caution	The sequence AAA41233.1 differs from that shown. Reason: Chimeric cDNA.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	GTP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	succinate metabolic process Inferred from direct assay PubMed 17403370. Source: RGD succinyl-CoA metabolic process Inferred from direct assay PubMed 17403370PubMed 7430155. Source: RGD tricarboxylic acid cycle Inferred from direct assay PubMed 17403370. Source: RGD
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara succinate-CoA ligase complex (GDP-forming) Inferred from direct assay PubMed 7430155. Source: RGD
Molecular_function	ATP citrate synthase activity Inferred from electronic annotation. Source: InterPro GDP binding Inferred from direct assay PubMed 7430155. Source: RGD GTP binding Inferred from electronic annotation. Source: UniProtKB-KW cofactor binding Inferred from electronic annotation. Source: InterPro protein heterodimerization activity Inferred from direct assay PubMed 7430155. Source: RGD succinate-CoA ligase (ADP-forming) activity Inferred from direct assay PubMed 17403370. Source: RGD succinate-CoA ligase (GDP-forming) activity Inferred from direct assay PubMed 17403370PubMed 7430155. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Mitochondrion
	Chain	41 – 346	306	Succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial		PRO_0000033343
Sites
	Active site	299	1	Tele-phosphohistidine intermediate Ref.2
Amino acid modifications
	Modified residue	54	1	N6-acetyllysine By similarity
	Cross-link	280		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Experimental info
	Sequence conflict	153	1	V → L in AAA41233. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P13086 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 2DDFC746C11B5B0C	FASTA	346	36,148
	10 20 30 40 50 60 MTAAVVAAAA TATMVSGSSG LAAARLLSRT FLLQQNGIRH GSYTASRKNI YIDKNTKVIC 70 80 90 100 110 120 QGFTGKQGTF HSQQALEYGT KLVGGTTPGK GGKKHLGLPV FNTVKEAKEK TGATASVIYV 130 140 150 160 170 180 PPPFAAAAIN EAIDAEIPLV VCITEGIPQQ DMVRVKHKLT RQGKTRLIGP NCPGIINPGE 190 200 210 220 230 240 CKIGIMPGHI HKKGRIGIVS RSGTLTYEAV HQTTQVGLGQ SLCIGIGGDP FNGTNFIDCL 250 260 270 280 290 300 DVFLKDPATE GIVLIGEIGG HAEENAAEFL KEHNSGPKAK PVVSFIAGIT APPGRRMGHA 310 320 330 340 GAIIAGGKGG AKEKISALQS AGVIVSMSPA QLGTCMYKEF EKRKML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[2]	"Cloning and sequencing of the cytoplasmic precursor to the alpha subunit of rat liver mitochondrial succinyl-CoA synthetase." Henning W.D., Upton C., McFadden G., Majumdar R., Bridger W.A. Proc. Natl. Acad. Sci. U.S.A. 85:1432-1436(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-346. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC061537 mRNA. Translation: AAH61537.2. J03621 mRNA. Translation: AAA41233.1. Sequence problems.
IPI	IPI00197555.
PIR	SYRTSA. A28962.
RefSeq	NP_446204.2. NM_053752.2.
UniGene	Rn.3766.
3D structure databases
ProteinModelPortal	P13086.
SMR	P13086. Positions 42-346.
ModBase	Search...
Protein-protein interaction databases
IntAct	P13086. 1 interaction.
PTM databases
PhosphoSite	P13086.
Proteomic databases
PaxDb	P13086.
PRIDE	P13086.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000007624; ENSRNOP00000007624; ENSRNOG00000005587.
GeneID	114597.
KEGG	rno:114597.
UCSC	RGD:619821. rat.
Organism-specific databases
CTD	8802.
RGD	619821. Suclg1.
Phylogenomic databases
eggNOG	COG0074.
GeneTree	ENSGT00530000063275.
HOGENOM	HOG000239685.
HOVERGEN	HBG000957.
InParanoid	Q6P7S4.
KO	K01899.
OrthoDB	EOG4WQ136.
Enzyme and pathway databases
UniPathway	UPA00223.
Gene expression databases
Genevestigator	P13086.
GermOnline	ENSRNOG00000005587. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.50.261. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR017440. Cit_synth/succinyl-CoA_lig_AS. IPR003781. CoA-bd. IPR005810. CoA_lig_alpha. IPR005811. CoA_ligase. IPR016040. NAD(P)-bd_dom. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
Pfam	PF02629. CoA_binding. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PIRSF	PIRSF001553. SucCS_alpha. 1 hit.
PRINTS	PR01798. SCOASYNTHASE.
SMART	SM00881. CoA_binding. 1 hit. [Graphical view]
SUPFAM	SSF52210. CoA_ligase. 1 hit.
TIGRFAMs	TIGR01019. sucCoAalpha. 1 hit.
PROSITE	PS01216. SUCCINYL_COA_LIG_1. 1 hit. PS00399. SUCCINYL_COA_LIG_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	618767.

Entry information

Entry name

SUCA_RAT

Accession

Primary (citable) accession number: P13086
Secondary accession number(s): Q6P7S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 10, 2009
Last modified:	April 3, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program