P13085 (PTHR_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Parathyroid hormone-related protein
Short name=PTH-rP
Short name=PTHrP

Alternative name(s):
Parathyroid hormone-like protein
Short name=PLP

Cleaved into the following chain:

1. Osteostatin

Gene names

Name:	Pthlh
Synonyms:	Pthrp

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teethRequired for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath By similarity. Osteostatin is a potent inhibitor of osteoclastic bone resorption By similarity.
Subunit structure	PTHrP interacts with PTH1R (via N-terminal extracellular domain) By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Secreted By similarity.
Post-translational modification	There are several secretory forms, including osteostatin, arising from endoproteolytic cleavage of the initial translation product. Each of these secretory forms is believed to have one or more of its own receptors that mediates the normal paracrine, autocrine and endocrine actions By similarity.
Sequence similarities	Belongs to the parathyroid hormone family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Secreted
Domain	Signal
Ligand	Calcium
Molecular function	Hormone
PTM	Cleavage on pair of basic residues
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from direct assay Ref.1. Source: RGD osteoblast development Traceable author statement PubMed 12706025. Source: RGD regulation of chondrocyte differentiation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hormone activity Inferred from direct assay Ref.1. Source: RGD peptide hormone receptor binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 34	10			PRO_0000023235
	Chain	37 – 177	141	Parathyroid hormone-related protein		PRO_0000023236
	Peptide	143 – 175	33	Osteostatin By similarity		PRO_0000023237
Regions
	Region	57 – 68	12	Important for receptor binding By similarity
	Motif	108 – 129	22	Nuclear localization signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13085 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 11091EC48CA73B20	FASTA	177	20,204
	10 20 30 40 50 60 MLRRLVQQWS VLVFLLSYSV PSRGRSVEGL GRRLKRAVSE HQLLHDKGKS IQDLRRRFFL 70 80 90 100 110 120 HHLIAEIHTA EIRATSEVSP NSKPAPNTKN HPVRFGSDDE GRYLTQETNK VETYKEQPLK 130 140 150 160 170 TPGKKKKGKP GKRREQEKKK RRTRSAWPGT TGSGLLEDPQ PHTSPTSTSL EPSSRTH

References

[1]	"Expression of a calcium-mobilizing parathyroid hormone-like peptide in lactating mammary tissue." Thiede M.A., Rodan G.A. Science 242:278-280(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Rat parathyroid hormone-like peptide: comparison with the human homologue and expression in malignant and normal tissue." Yasuda T., Banville D., Rabbani S.A., Hendy G.N., Goltzman D. Mol. Endocrinol. 3:518-525(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Gene-encoding parathyroid hormone-like peptide: nucleotide sequence of the rat gene and comparison with the human homologue." Karaplis A.C., Yasuda T., Hendy G.N., Goltzman D., Banville D. Mol. Endocrinol. 4:441-446(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21967 mRNA. Translation: AAA41981.1. M31603 mRNA. Translation: AAA41980.1. M34112, M34108, M34111 Genomic DNA. Translation: AAA41889.1.
IPI	IPI00197554.
PIR	A30012. A34723.
RefSeq	NP_036768.1. NM_012636.1.
UniGene	Rn.9750.
3D structure databases
ProteinModelPortal	P13085.
SMR	P13085. Positions 37-70, 103-130.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000052327.
PTM databases
PhosphoSite	P13085.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24695.
KEGG	rno:24695.
UCSC	RGD:3441. rat.
Organism-specific databases
CTD	5744.
RGD	3441. Pthlh.
Phylogenomic databases
eggNOG	opiNOG07545.
HOVERGEN	HBG003236.
OrthoDB	EOG4N8R63.
Gene expression databases
ArrayExpress	P13085.
Genevestigator	P13085.
Family and domain databases
InterPro	IPR003626. PTH-rel. IPR001415. PTH/PTH-rel. [Graphical view]
PANTHER	PTHR17223. PTHR17223. 1 hit.
Pfam	PF01279. Parathyroid. 1 hit. [Graphical view]
SMART	SM00087. PTH. 1 hit. [Graphical view]
PROSITE	PS00335. PARATHYROID. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604147.

Entry information

Entry name

PTHR_RAT

Accession

Primary (citable) accession number: P13085

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program