Reviewed, UniProtKB/Swiss-Prot P13066 (PHSL_DESNO)

Names and origin

Protein names	Recommended name:     Periplasmic [NiFeSe] hydrogenase large subunit     EC=1.12.99.6 Alternative name(s):     NiFeSe hydrogenlyase large chain
Organism	Desulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))
Taxonomic identifier	52561 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfomicrobiaceae › Desulfomicrobium

Protein attributes

Sequence length	19 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	H2 + A = AH2.
Cofactor	Nickel. Iron.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Periplasm
Ligand	Iron Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›19	›19	Periplasmic [NiFeSe] hydrogenase large subunit		PRO_0000199705
Experimental info
	Non-terminal residue	19	1

Sequences

	Sequence		Length	Mass (Da)
	P13066-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 2BFCD2D360F00367	FASTA	19	1,942
	10 AQAATPAADG KKKISIDTR

References

[1]

"Identification of three classes of hydrogenase in the genus, Desulfovibrio." Prickril B.C., He S.H., Li C., Menon N.K., Choi E.S., Przybyla A.E., Dervartanian D.V., Peck H.D. Jr., Fauque G., le Gall J., Teixeira M., Moura I., Moura J.J.G., Patil D., Huynh B.H. Biochem. Biophys. Res. Commun. 149:369-377(1987) [PubMed: 3322275] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	H27480.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 259090.
Family and domain databases
PROSITE	PS00507. NI_HGENASE_L_1. Partial match. PS00508. NI_HGENASE_L_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHSL_DESNO

Accession

Primary (citable) accession number: P13066

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)