Reviewed, UniProtKB/Swiss-Prot P13064 (PHSS_DESNO)

Names and origin

Protein names	Recommended name:     Periplasmic [NiFeSe] hydrogenase small subunit     EC=1.12.99.6 Alternative name(s):     NiFeSe hydrogenlyase small chain
Organism	Desulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))
Taxonomic identifier	52561 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfomicrobiaceae › Desulfomicrobium

Protein attributes

Sequence length	32 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Periplasm
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›32	›32	Periplasmic [NiFeSe] hydrogenase small subunit		PRO_0000204355
Sites
	Metal binding	18	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	21	1	Iron-sulfur 1 (4Fe-4S) By similarity
Experimental info
	Non-terminal residue	32	1

Sequences

	Sequence		Length	Mass (Da)
	P13064-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 7001FAAA174C8199	FASTA	32	3,305
	10 20 30 MTQGAKKAPV IWVQGEGCTG CSVSLLNAVC PR

References

[1]

"Identification of three classes of hydrogenase in the genus, Desulfovibrio." Prickril B.C., He S.H., Li C., Menon N.K., Choi E.S., Przybyla A.E., Dervartanian D.V., Peck H.D. Jr., Fauque G., le Gall J., Teixeira M., Moura I., Moura J.J.G., Patil D., Huynh B.H. Biochem. Biophys. Res. Commun. 149:369-377(1987) [PubMed: 3322275] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1CC1 based on UniProtKB P13063.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 259090.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PHSS_DESNO

Accession

Primary (citable) accession number: P13064

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)