Reviewed, UniProtKB/Swiss-Prot P13062 (PHNS_DESMU)

Names and origin

Protein names

Recommended name:
    Periplasmic [NiFe] hydrogenase small subunit
    EC=1.12.2.1
Alternative name(s):
    NiFe hydrogenlyase small chain

Gene names

Name:

hydA

Organism

Desulfovibrio multispirans

Taxonomic identifier

32018 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	32 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 H2 + ferricytochrome c3 = 4 H+ + ferrocytochrome c3.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Periplasm
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW cytochrome-c3 hydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›32	›32	Periplasmic [NiFe] hydrogenase small subunit		PRO_0000204354
Sites
	Metal binding	17	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	20	1	Iron-sulfur 1 (4Fe-4S) By similarity
Experimental info
	Non-terminal residue	32	1

Sequences

	Sequence		Length	Mass (Da)
	P13062-1 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: A6D20619B0B011EB	FASTA	32	3,504
	10 20 30 ALTGRRPSVV YLHAAQCTGC SEALLRTTKP FR

References

[1]

"Identification of three classes of hydrogenase in the genus, Desulfovibrio." Prickril B.C., He S.H., Li C., Menon N.K., Choi E.S., Przybyla A.E., Dervartanian D.V., Peck H.D. Jr., Fauque G., le Gall J., Teixeira M., Moura I., Moura J.J.G., Patil D., Huynh B.H. Biochem. Biophys. Res. Commun. 149:369-377(1987) [PubMed: 3322275] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	E27480.
3D structure databases
HSSP	HSSP built from PDB template 1FRV based on UniProtKB P12943.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.2.1. 258180.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PHNS_DESMU

Accession

Primary (citable) accession number: P13062

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)