P13062 (PHNS_DESMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic [NiFe] hydrogenase small subunit
EC=1.12.2.1

Alternative name(s):
NiFe hydrogenlyase small chain

Gene names

Name:

hydA

Organism

Desulfovibrio multispirans

Taxonomic identifier

32018 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	32 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2 H2 + ferricytochrome c3 = 4 H+ + ferrocytochrome c3.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Periplasm
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro cytochrome-c3 hydrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›32	›32	Periplasmic [NiFe] hydrogenase small subunit		PRO_0000204354
Sites
	Metal binding	17	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	20	1	Iron-sulfur 1 (4Fe-4S) By similarity
Experimental info
	Non-terminal residue	32	1

Sequences

	Sequence		Length	Mass (Da)
	P13062 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: A6D20619B0B011EB	FASTA	32	3,504
	10 20 30 ALTGRRPSVV YLHAAQCTGC SEALLRTTKP FR

References

[1]	"Identification of three classes of hydrogenase in the genus, Desulfovibrio." Prickril B.C., He S.H., Li C., Menon N.K., Choi E.S., Przybyla A.E., Dervartanian D.V., Peck H.D. Jr., Fauque G., le Gall J., Teixeira M., Moura I., Moura J.J.G., Patil D., Huynh B.H. Biochem. Biophys. Res. Commun. 149:369-377(1987) [PubMed: 3322275] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	E27480.
3D structure databases
ProteinModelPortal	P13062.
SMR	P13062. Positions 6-31.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNS_DESMU

Accession

Primary (citable) accession number: P13062

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1995
Last modified:	June 28, 2011
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program