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Protein

Elongation factor 2

Gene

EF2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338GTPBy similarity
Nucleotide bindingi108 – 1125GTPBy similarity
Nucleotide bindingi162 – 1654GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: FlyBase

GO - Biological processi

  • mitotic spindle elongation Source: FlyBase
  • mitotic spindle organization Source: FlyBase
  • translation Source: GO_Central
  • translational elongation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-156902. Peptide chain elongation.
R-DME-5358493. Synthesis of diphthamide-EEF2.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:EF2
Synonyms:Ef2b
ORF Names:CG2238
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000559. EF2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: FlyBase
  • lipid particle Source: FlyBase
  • microtubule associated complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 844843Elongation factor 2PRO_0000091012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571PhosphothreonineBy similarity
Modified residuei59 – 591PhosphothreonineBy similarity
Modified residuei701 – 7011DiphthamideBy similarity

Post-translational modificationi

Phosphorylation by EF-2 kinase completely inactivates EF-2.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13060.
PRIDEiP13060.

Expressioni

Developmental stagei

Expression commences during embryonic germ band elongation and persists throughout development and in the adult. Highest level of expression observed in late embryonic, late larval and early pupal stages.1 Publication

Gene expression databases

BgeeiFBgn0000559.
GenevisibleiP13060. DM.

Interactioni

Protein-protein interaction databases

BioGridi61367. 67 interactions.
DIPiDIP-19881N.
IntActiP13060. 6 interactions.
MINTiMINT-306310.
STRINGi7227.FBpp0305182.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Welectron microscopy6.00Az1-844[»]
ProteinModelPortaliP13060.
SMRiP13060. Positions 2-844.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 348332tr-type GPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0469. Eukaryota.
COG0480. LUCA.
GeneTreeiENSGT00840000129841.
HOGENOMiHOG000248060.
InParanoidiP13060.
KOiK03234.
OMAiFITHADQ.
OrthoDBiEOG091G0A2J.
PhylomeDBiP13060.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNFTVDEIR GLMDKKRNIR NMSVIAHVDH GKSTLTDSLV SKAGIIAGAK
60 70 80 90 100
AGETRFTDTR KDEQERCITI KSTAISMYFE VEEKDLVFIT HPDQREKECK
110 120 130 140 150
GFLINLIDSP GHVDFSSEVT AALRVTDGAL VVVDCVSGVC VQTETVLRQA
160 170 180 190 200
IAERIKPILF MNKMDRALLE LQLDAEELYQ TFQRIVENVN VIIATYNDDG
210 220 230 240 250
GPMGEVRVDP SKGSVGFGSG LHGWAFTLKQ FSEMYSEKFK IDVVKLMNRL
260 270 280 290 300
WGENFFNAKT KKWQKQKEAD NKRSFCMYIL DPIYKVFDAI MNYKKEEIGT
310 320 330 340 350
LLEKIGVTLK HEDKDKDGKA LLKTVMRTWL PAGEALLQMI AIHLPSPVVA
360 370 380 390 400
QKYRMEMLYE GPHDDEAAIA VKSCDPDGPL MMYISKMVPT SDKGRFYAFG
410 420 430 440 450
RVFAGKVATG QKCRIMGPNY TPGKKEDLYE KAIQRTILMM GRYVEAIEDV
460 470 480 490 500
PSGNICGLVG VDQFLVKTGT ITTFKDAHNM KVMKFSVSPV VRVAVEPKNP
510 520 530 540 550
ADLPKLVEGL KRLAKSDPMV QCIIEESGEH IIAGAGELHL EICLKDLEED
560 570 580 590 600
HACIPLKKSD PVVSYRETVS EESDQMCLSK SPNKHNRLLM KALPMPDGLP
610 620 630 640 650
EDIDNGDVSA KDEFKARARY LSEKYDYDVT EARKIWCFGP DGTGPNFILD
660 670 680 690 700
CTKSVQYLNE IKDSVVAGFQ WASKEGILAD ENLRGVRFNI YDVTLHADAI
710 720 730 740 750
HRGGGQIIPT TRRCLYAAAI TAKPRLMEPV YLCEIQCPEV AVGGIYGVLN
760 770 780 790 800
RRRGHVFEEN QVVGTPMFVV KAYLPVNESF GFTADLRSNT GGQAFPQCVF
810 820 830 840
DHWQVLPGDP SEPSSKPYAI VQDTRKRKGL KEGLPDLSQY LDKL
Length:844
Mass (Da):94,459
Last modified:January 23, 2007 - v4
Checksum:i547A6ED0658B6B6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti607 – 6071D → E in CAA33804 (PubMed:2508059).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15805 mRNA. Translation: CAA33804.1.
AE014134 Genomic DNA. Translation: AAF57226.2.
AE014134 Genomic DNA. Translation: AAG22125.3.
AE014134 Genomic DNA. Translation: AAN11135.2.
AY075481 mRNA. Translation: AAL68292.1.
PIRiS05988.
RefSeqiNP_525105.2. NM_080366.3.
NP_724357.2. NM_165394.3.
NP_724358.2. NM_165395.2.
UniGeneiDm.6600.

Genome annotation databases

EnsemblMetazoaiFBtr0085911; FBpp0085265; FBgn0000559.
FBtr0085912; FBpp0085266; FBgn0000559.
FBtr0332966; FBpp0305182; FBgn0000559.
GeneIDi35422.
KEGGidme:Dmel_CG2238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15805 mRNA. Translation: CAA33804.1.
AE014134 Genomic DNA. Translation: AAF57226.2.
AE014134 Genomic DNA. Translation: AAG22125.3.
AE014134 Genomic DNA. Translation: AAN11135.2.
AY075481 mRNA. Translation: AAL68292.1.
PIRiS05988.
RefSeqiNP_525105.2. NM_080366.3.
NP_724357.2. NM_165394.3.
NP_724358.2. NM_165395.2.
UniGeneiDm.6600.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Welectron microscopy6.00Az1-844[»]
ProteinModelPortaliP13060.
SMRiP13060. Positions 2-844.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61367. 67 interactions.
DIPiDIP-19881N.
IntActiP13060. 6 interactions.
MINTiMINT-306310.
STRINGi7227.FBpp0305182.

Proteomic databases

PaxDbiP13060.
PRIDEiP13060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085911; FBpp0085265; FBgn0000559.
FBtr0085912; FBpp0085266; FBgn0000559.
FBtr0332966; FBpp0305182; FBgn0000559.
GeneIDi35422.
KEGGidme:Dmel_CG2238.

Organism-specific databases

CTDi35422.
FlyBaseiFBgn0000559. EF2.

Phylogenomic databases

eggNOGiKOG0469. Eukaryota.
COG0480. LUCA.
GeneTreeiENSGT00840000129841.
HOGENOMiHOG000248060.
InParanoidiP13060.
KOiK03234.
OMAiFITHADQ.
OrthoDBiEOG091G0A2J.
PhylomeDBiP13060.

Enzyme and pathway databases

ReactomeiR-DME-156902. Peptide chain elongation.
R-DME-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

ChiTaRSiEf2b. fly.
GenomeRNAii35422.
PROiP13060.

Gene expression databases

BgeeiFBgn0000559.
GenevisibleiP13060. DM.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF2_DROME
AccessioniPrimary (citable) accession number: P13060
Secondary accession number(s): A0A023GPJ5, Q9I7H2, Q9V9R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 163 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.