Reviewed, UniProtKB/Swiss-Prot P13054 (KNRL_DROME)

Names and origin

Protein names

Recommended name:
    Knirps-related protein
Alternative name(s):
    Nuclear receptor subfamily 0 group A member 2

Gene names

Name:	knrl
Synonyms:	NR0A2
ORF Names:	CG4761

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Nucleus.
Sequence similarities	Belongs to the nuclear hormone receptor family. NR0 subfamily. Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Receptor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched duct epithelial cell fate determination, open tracheal system Traceable author statement. Source: FlyBase epithelial cell migration, open tracheal system Traceable author statement. Source: FlyBase regulation of mitosis Inferred from mutant phenotype. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW regulation of tube size, open tracheal system Traceable author statement. Source: FlyBase transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ligand-dependent nuclear receptor activity Non-traceable author statement. Source: FlyBase protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CtBP	O46036	1	EBI-134637,EBI-159330
G-oalpha47A	P16378-2	1	EBI-134637,EBI-197479

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	Knirps-related protein		PRO_0000053745
Regions
	DNA binding	11 – 87	77	Nuclear receptor
	Zinc finger	14 – 34	21	NR C4-type
	Zinc finger	51 – 75	25	NR C4-type
	Compositional bias	121 – 262	142	Gly-rich
	Compositional bias	577 – 597	21	Asn-rich

Sequences

	Sequence		Length	Mass (Da)
	P13054-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: E466FA081DAACDC8	FASTA	647	68,322
	10 20 30 40 50 60 MMNQDNPYAM NQTCKVCGEP AAGFHFGAFT CEGCKSFFGR SYNNLSSISD CKNNGECIIN 70 80 90 100 110 120 KKNRTACKAC RLKKCLMVGM SKSGSRYGRR SNWFKIHCLL QEQQQQAVAA MAAHHNSQQA 130 140 150 160 170 180 GGGSSGGSGG GQGMPNGVKG MSGVPPPAAA AAALGMLGHP GGYPGLYAVA NAGGSSRSKE 190 200 210 220 230 240 ELMMLGLDGS VEYGSHKHPV VASPSVSSPD SHNSDSSVEV SSVRGNPLLH LGGKSNSGGS 250 260 270 280 290 300 SSGADGSHSG GGGGGGGGVT PGRPPQMRKD LSPFLPLPFP GLASMPVMPP PAFLPPSHLL 310 320 330 340 350 360 FPGYHPALYS HHQGLLKPTP EQQQAAVAAA AVQHLFNSSG AGQRFAPGTS PFANHQQHHK 370 380 390 400 410 420 EEDQPAPARS PSTHANNNHL LTNGGAADEL TKRFYLDAVL KSQQQSPPPT TKLPPHSKQD 430 440 450 460 470 480 YSISALVTPN SESGRERVKS RQNEEDDEAR ADGIIDGAEH DDEEEDLVVS MTPPHSPAQQ 490 500 510 520 530 540 EERTPAGEDP RPSPGQDNPI DLSMKTTGSS LSSKSSSPEI EPETEISSDV EKNDTDDDDE 550 560 570 580 590 600 DLKVTPEEEI SVRETADPEI EEDHSSTTET AKTSIENTHN NNNSISNNNN NNNNNNNSIL 610 620 630 640 SDSEASETIK RKLDELIEAS SENGKRLRLE APVKVATSNA LDLTTKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila gene knirps-related is a member of the steroid-receptor gene superfamily." Oro A.E., Ong E.S., Margolis J.S., Posakony J.W., McKeown M., Evans R.M. Nature 336:493-496(1988) [PubMed: 2848202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X14153 Genomic DNA. Translation: CAA32365.1. AE014296 Genomic DNA. Translation: AAF51627.2. AY075383 mRNA. Translation: AAL68221.1.
PIR	S06450.
RefSeq	NP_788552.1.
UniGene	Dm.1017
3D structure databases
HSSP	HSSP built from PDB template 1KB2 based on UniProtKB P11473.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:20216N.
IntAct	P13054. 7 interactions.
Proteomic databases
PRIDE	P13054.
Genome annotation databases
Ensembl	FBgn0001323. Drosophila melanogaster. [Contig view]
GeneID	40285.
KEGG	dme:Dmel_CG4761.
NMPDR	fig|7227.3.peg.10785.
Organism-specific databases
FlyBase	FBgn0001323. knrl.
Phylogenomic databases
HOGENOM	P13054.
OMA	P13054. TPNSESG.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-017588-MON.
Gene expression databases
ArrayExpress	P13054.
GermOnline	CG4761. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000324. VitD_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Gene3D	G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.
Pfam	PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR00047. STROIDFINGER. PR00350. VITAMINDR.
ProDom	PD000035. Znf_C4steroid. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00399. ZnF_C4. 1 hit. [Graphical view]
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	817980.

Entry information

Entry name

KNRL_DROME

Accession

Primary (citable) accession number: P13054
Secondary accession number(s): Q9VPC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project