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Protein

Protein GAL3

Gene

GAL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • galactose metabolic process Source: SGD
  • maintenance of protein location Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD

Keywordsi

Biological processCarbohydrate metabolism, Galactose metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29629-MONOMER
ReactomeiR-SCE-70370 Galactose catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GAL3
Gene namesi
Name:GAL3
Ordered Locus Names:YDR009W
ORF Names:YD8119.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR009W
SGDiS000002416 GAL3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001846581 – 520Protein GAL3Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP13045
PaxDbiP13045
PRIDEiP13045

PTM databases

iPTMnetiP13045

Expressioni

Inductioni

The GAL3 gene is a member of the family of galactose inducible, glucose-repressible genes (which include GAL1, GAL2, GAL7, GAL10, GAL80, and MELI).

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GAL80P043875EBI-7282,EBI-2061197

Protein-protein interaction databases

BioGridi32061, 64 interactors
DIPiDIP-5725N
IntActiP13045, 9 interactors
MINTiP13045
STRINGi4932.YDR009W

Structurei

Secondary structure

1520
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Helixi20 – 34Combined sources15
Beta strandi39 – 51Combined sources13
Helixi56 – 58Combined sources3
Beta strandi62 – 77Combined sources16
Beta strandi84 – 91Combined sources8
Beta strandi97 – 100Combined sources4
Beta strandi112 – 114Combined sources3
Helixi119 – 135Combined sources17
Helixi137 – 140Combined sources4
Beta strandi141 – 143Combined sources3
Beta strandi148 – 154Combined sources7
Helixi162 – 179Combined sources18
Helixi187 – 195Combined sources9
Helixi198 – 201Combined sources4
Helixi208 – 215Combined sources8
Beta strandi220 – 225Combined sources6
Beta strandi227 – 229Combined sources3
Beta strandi231 – 236Combined sources6
Beta strandi244 – 251Combined sources8
Helixi258 – 261Combined sources4
Turni263 – 265Combined sources3
Helixi266 – 283Combined sources18
Beta strandi296 – 299Combined sources4
Helixi302 – 313Combined sources12
Helixi324 – 342Combined sources19
Helixi343 – 345Combined sources3
Helixi351 – 357Combined sources7
Helixi362 – 369Combined sources8
Beta strandi370 – 373Combined sources4
Beta strandi378 – 380Combined sources3
Helixi382 – 404Combined sources23
Helixi411 – 431Combined sources21
Helixi438 – 449Combined sources12
Beta strandi453 – 473Combined sources21
Helixi478 – 488Combined sources11
Helixi490 – 493Combined sources4
Helixi499 – 505Combined sources7
Beta strandi506 – 509Combined sources4
Beta strandi515 – 519Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V2UX-ray2.10C/D2-520[»]
3V5RX-ray2.10A/B17-520[»]
ProteinModelPortaliP13045
SMRiP13045
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063433
HOGENOMiHOG000241099
InParanoidiP13045
KOiK00849
OMAiHQEIDLQ
OrthoDBiEOG092C193C

Family and domain databases

Gene3Di3.30.230.10, 2 hits
3.30.70.890, 1 hit
InterProiView protein in InterPro
IPR000705 Galactokinase
IPR019741 Galactokinase_CS
IPR019539 GalKase_gal-bd
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR006206 Mevalonate/galactokinase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF10509 GalKase_gal_bdg, 1 hit
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF000530 Galactokinase, 1 hit
PRINTSiPR00473 GALCTOKINASE
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 2 hits
TIGRFAMsiTIGR00131 gal_kin, 1 hit
PROSITEiView protein in PROSITE
PS00106 GALACTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P13045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTNVPIFSS PVRDLPRSFE QKHLAVVDAF FQTYHVKPDF IARSPGRVNL
60 70 80 90 100
IGEHIDYCDF SVLPLAIDVD MLCAVKILDE KNPSITLTNA DPKFAQRKFD
110 120 130 140 150
LPLDGSYMAI DPSVSEWSNY FKCGLHVAHS YLKKIAPERF NNTPLVGAQI
160 170 180 190 200
FCQSDIPTGG GLSSAFTCAA ALATIRANMG KNFDISKKDL TRITAVAEHY
210 220 230 240 250
VGVNNGGMDQ ATSVYGEEDH ALYVEFRPKL KATPFKFPQL KNHEISFVIA
260 270 280 290 300
NTLVKSNKFE TAPTNYNLRV IEVTVAANAL ATRYSVALPS HKDNSNSERG
310 320 330 340 350
NLRDFMDAYY ARYENQAQPW NGDIGTGIER LLKMLQLVEE SFSRKKSGFT
360 370 380 390 400
VHEASTALNC SREEFTRDYL TTFPVRFQVL KLYQRAKHVY SESLRVLKAL
410 420 430 440 450
KMMTSATFHT DEDFFTDFGR LMNESQASCD KLYECSCIET NQICSIALAN
460 470 480 490 500
GSFGSRLTGA GWGGCTIHLV PSGANGNVEQ VRKALIEKFY NVRYPDLTDE
510 520
ELKDAIIVSK PALGTCLYEQ
Length:520
Mass (Da):58,129
Last modified:February 1, 1996 - v2
Checksum:i5852028EBDDC0F3B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37 – 45KPDFIARSP → NLILSLGLL in AAA34625 (PubMed:3062381).Curated9
Sequence conflicti170 – 171AA → GR in AAA34625 (PubMed:3062381).Curated2
Sequence conflicti192 – 211RITAV…GMDQA → ASQRLRSTMLESIMVVWIKQ in AAA34625 (PubMed:3062381).CuratedAdd BLAST20
Sequence conflicti227 – 241RPKLK…FPQLK → MAKTKWPHFQVSSIE in AAA34625 (PubMed:3062381).CuratedAdd BLAST15
Sequence conflicti251 – 294NTLVK…SHKDN → ILCTRSNNRTLLHISLSCSL LTLLYISTFAREPCGPDTLG LTISQGQ in AAA34625 (PubMed:3062381).CuratedAdd BLAST44
Sequence conflicti303 – 338RDFMD…MLQLV → EIYGCLLRPDTKTKPNHGME ISELVLNVYSRCYNWY in AAA34625 (PubMed:3062381).CuratedAdd BLAST36
Sequence conflicti407 – 425TFHTD…LMNES → IFTRTRFLYRFWPTNE in AAA34625 (PubMed:3062381).CuratedAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21615 Genomic DNA Translation: AAA34625.1
X95966 Genomic DNA Translation: CAA65201.1
Z48008 Genomic DNA Translation: CAA88069.1
Z74305 Genomic DNA Translation: CAA98829.1
AY723766 Genomic DNA Translation: AAU09683.1
BK006938 Genomic DNA Translation: DAA11856.1
PIRiS50990
RefSeqiNP_010292.1, NM_001180317.1

Genome annotation databases

EnsemblFungiiYDR009W; YDR009W; YDR009W
GeneIDi851572
KEGGisce:YDR009W

Similar proteinsi

Entry informationi

Entry nameiGAL3_YEAST
AccessioniPrimary (citable) accession number: P13045
Secondary accession number(s): D6VRZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health