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P13045 (GAL3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein GAL3
Gene names
Name:GAL3
Ordered Locus Names:YDR009W
ORF Names:YD8119.14
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.

Induction

The GAL3 gene is a member of the family of galactose inducible, glucose-repressible genes (which include GAL1, GAL2, GAL7, GAL10, GAL80, and MELI).

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GHMP kinase family. GalK subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

URE2P232021EBI-7282,EBI-20138

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520Protein GAL3
PRO_0000184658

Amino acid modifications

Modified residue11N-acetylmethionine Ref.7

Experimental info

Sequence conflict37 – 459KPDFIARSP → NLILSLGLL in AAA34625. Ref.1
Sequence conflict170 – 1712AA → GR in AAA34625. Ref.1
Sequence conflict192 – 21120RITAV…GMDQA → ASQRLRSTMLESIMVVWIKQ in AAA34625. Ref.1
Sequence conflict227 – 24115RPKLK…FPQLK → MAKTKWPHFQVSSIE in AAA34625. Ref.1
Sequence conflict251 – 29444NTLVK…SHKDN → ILCTRSNNRTLLHISLSCSL LTLLYISTFAREPCGPDTLG LTISQGQ in AAA34625. Ref.1
Sequence conflict303 – 33836RDFMD…MLQLV → EIYGCLLRPDTKTKPNHGME ISELVLNVYSRCYNWY in AAA34625. Ref.1
Sequence conflict407 – 42519TFHTD…LMNES → IFTRTRFLYRFWPTNE in AAA34625. Ref.1

Secondary structure

............................................................................ 520
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13045 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 5852028EBDDC0F3B

FASTA52058,129
        10         20         30         40         50         60 
MNTNVPIFSS PVRDLPRSFE QKHLAVVDAF FQTYHVKPDF IARSPGRVNL IGEHIDYCDF 

        70         80         90        100        110        120 
SVLPLAIDVD MLCAVKILDE KNPSITLTNA DPKFAQRKFD LPLDGSYMAI DPSVSEWSNY 

       130        140        150        160        170        180 
FKCGLHVAHS YLKKIAPERF NNTPLVGAQI FCQSDIPTGG GLSSAFTCAA ALATIRANMG 

       190        200        210        220        230        240 
KNFDISKKDL TRITAVAEHY VGVNNGGMDQ ATSVYGEEDH ALYVEFRPKL KATPFKFPQL 

       250        260        270        280        290        300 
KNHEISFVIA NTLVKSNKFE TAPTNYNLRV IEVTVAANAL ATRYSVALPS HKDNSNSERG 

       310        320        330        340        350        360 
NLRDFMDAYY ARYENQAQPW NGDIGTGIER LLKMLQLVEE SFSRKKSGFT VHEASTALNC 

       370        380        390        400        410        420 
SREEFTRDYL TTFPVRFQVL KLYQRAKHVY SESLRVLKAL KMMTSATFHT DEDFFTDFGR 

       430        440        450        460        470        480 
LMNESQASCD KLYECSCIET NQICSIALAN GSFGSRLTGA GWGGCTIHLV PSGANGNVEQ 

       490        500        510        520 
VRKALIEKFY NVRYPDLTDE ELKDAIIVSK PALGTCLYEQ 

« Hide

References

« Hide 'large scale' references
[1]"Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases."
Bajwa W., Torchia T.E., Hopper J.E.
Mol. Cell. Biol. 8:3439-3447(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-425.
[2]"Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames."
Eide L.G., Sander C., Prydz H.
Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M21615 Genomic DNA. Translation: AAA34625.1.
X95966 Genomic DNA. Translation: CAA65201.1.
Z48008 Genomic DNA. Translation: CAA88069.1.
Z74305 Genomic DNA. Translation: CAA98829.1.
AY723766 Genomic DNA. Translation: AAU09683.1.
BK006938 Genomic DNA. Translation: DAA11856.1.
PIRS50990.
RefSeqNP_010292.1. NM_001180317.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3V2UX-ray2.10C/D2-520[»]
3V5RX-ray2.10A/B17-520[»]
ProteinModelPortalP13045.
SMRP13045. Positions 2-520.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32061. 47 interactions.
DIPDIP-5725N.
IntActP13045. 2 interactions.
MINTMINT-4480213.
STRING4932.YDR009W.

Proteomic databases

PaxDbP13045.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR009W; YDR009W; YDR009W.
GeneID851572.
KEGGsce:YDR009W.

Organism-specific databases

CYGDYDR009w.
SGDS000002416. GAL3.

Phylogenomic databases

eggNOGCOG0153.
GeneTreeENSGT00530000063433.
HOGENOMHOG000241099.
KOK00849.
OMACREAGAY.
OrthoDBEOG7FZ075.

Enzyme and pathway databases

BioCycYEAST:G3O-29629-MONOMER.

Gene expression databases

GenevestigatorP13045.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF000530. Galactokinase. 1 hit.
PRINTSPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsTIGR00131. gal_kin. 1 hit.
PROSITEPS00106. GALACTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969025.

Entry information

Entry nameGAL3_YEAST
AccessionPrimary (citable) accession number: P13045
Secondary accession number(s): D6VRZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references