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P13045

- GAL3_YEAST

UniProt

P13045 - GAL3_YEAST

Protein

Protein GAL3

Gene

GAL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. galactokinase activity Source: InterPro
    3. protein binding Source: SGD

    GO - Biological processi

    1. galactose metabolic process Source: SGD
    2. maintenance of protein location Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD

    Keywords - Biological processi

    Carbohydrate metabolism, Galactose metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29629-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein GAL3
    Gene namesi
    Name:GAL3
    Ordered Locus Names:YDR009W
    ORF Names:YD8119.14
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR009w.
    SGDiS000002416. GAL3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 520520Protein GAL3PRO_0000184658Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP13045.
    PaxDbiP13045.

    Expressioni

    Inductioni

    The GAL3 gene is a member of the family of galactose inducible, glucose-repressible genes (which include GAL1, GAL2, GAL7, GAL10, GAL80, and MELI).

    Gene expression databases

    GenevestigatoriP13045.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    URE2P232021EBI-7282,EBI-20138

    Protein-protein interaction databases

    BioGridi32061. 47 interactions.
    DIPiDIP-5725N.
    IntActiP13045. 2 interactions.
    MINTiMINT-4480213.
    STRINGi4932.YDR009W.

    Structurei

    Secondary structure

    1
    520
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 93
    Helixi20 – 3415
    Beta strandi39 – 5113
    Helixi56 – 583
    Beta strandi62 – 7716
    Beta strandi84 – 918
    Beta strandi97 – 1004
    Beta strandi112 – 1143
    Helixi119 – 13517
    Helixi137 – 1404
    Beta strandi141 – 1433
    Beta strandi148 – 1547
    Helixi162 – 17918
    Helixi187 – 1959
    Helixi198 – 2014
    Helixi208 – 2158
    Beta strandi220 – 2256
    Beta strandi227 – 2293
    Beta strandi231 – 2366
    Beta strandi244 – 2518
    Helixi258 – 2614
    Turni263 – 2653
    Helixi266 – 28318
    Beta strandi296 – 2994
    Helixi302 – 31312
    Helixi324 – 34219
    Helixi343 – 3453
    Helixi351 – 3577
    Helixi362 – 3698
    Beta strandi370 – 3734
    Beta strandi378 – 3803
    Helixi382 – 40423
    Helixi411 – 43121
    Helixi438 – 44912
    Beta strandi453 – 47321
    Helixi478 – 48811
    Helixi490 – 4934
    Helixi499 – 5057
    Beta strandi506 – 5094
    Beta strandi515 – 5195

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3V2UX-ray2.10C/D2-520[»]
    3V5RX-ray2.10A/B17-520[»]
    ProteinModelPortaliP13045.
    SMRiP13045. Positions 2-520.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GHMP kinase family. GalK subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0153.
    GeneTreeiENSGT00530000063433.
    HOGENOMiHOG000241099.
    KOiK00849.
    OMAiCREAGAY.
    OrthoDBiEOG7FZ075.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 2 hits.
    InterProiIPR000705. Galactokinase.
    IPR019741. Galactokinase_CS.
    IPR019539. GalKase_gal-bd.
    IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006206. Mevalonate/galactokinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PfamiPF10509. GalKase_gal_bdg. 1 hit.
    PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000530. Galactokinase. 1 hit.
    PRINTSiPR00473. GALCTOKINASE.
    PR00959. MEVGALKINASE.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 2 hits.
    TIGRFAMsiTIGR00131. gal_kin. 1 hit.
    PROSITEiPS00106. GALACTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13045-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTNVPIFSS PVRDLPRSFE QKHLAVVDAF FQTYHVKPDF IARSPGRVNL    50
    IGEHIDYCDF SVLPLAIDVD MLCAVKILDE KNPSITLTNA DPKFAQRKFD 100
    LPLDGSYMAI DPSVSEWSNY FKCGLHVAHS YLKKIAPERF NNTPLVGAQI 150
    FCQSDIPTGG GLSSAFTCAA ALATIRANMG KNFDISKKDL TRITAVAEHY 200
    VGVNNGGMDQ ATSVYGEEDH ALYVEFRPKL KATPFKFPQL KNHEISFVIA 250
    NTLVKSNKFE TAPTNYNLRV IEVTVAANAL ATRYSVALPS HKDNSNSERG 300
    NLRDFMDAYY ARYENQAQPW NGDIGTGIER LLKMLQLVEE SFSRKKSGFT 350
    VHEASTALNC SREEFTRDYL TTFPVRFQVL KLYQRAKHVY SESLRVLKAL 400
    KMMTSATFHT DEDFFTDFGR LMNESQASCD KLYECSCIET NQICSIALAN 450
    GSFGSRLTGA GWGGCTIHLV PSGANGNVEQ VRKALIEKFY NVRYPDLTDE 500
    ELKDAIIVSK PALGTCLYEQ 520
    Length:520
    Mass (Da):58,129
    Last modified:February 1, 1996 - v2
    Checksum:i5852028EBDDC0F3B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 459KPDFIARSP → NLILSLGLL in AAA34625. (PubMed:3062381)Curated
    Sequence conflicti170 – 1712AA → GR in AAA34625. (PubMed:3062381)Curated
    Sequence conflicti192 – 21120RITAV…GMDQA → ASQRLRSTMLESIMVVWIKQ in AAA34625. (PubMed:3062381)CuratedAdd
    BLAST
    Sequence conflicti227 – 24115RPKLK…FPQLK → MAKTKWPHFQVSSIE in AAA34625. (PubMed:3062381)CuratedAdd
    BLAST
    Sequence conflicti251 – 29444NTLVK…SHKDN → ILCTRSNNRTLLHISLSCSL LTLLYISTFAREPCGPDTLG LTISQGQ in AAA34625. (PubMed:3062381)CuratedAdd
    BLAST
    Sequence conflicti303 – 33836RDFMD…MLQLV → EIYGCLLRPDTKTKPNHGME ISELVLNVYSRCYNWY in AAA34625. (PubMed:3062381)CuratedAdd
    BLAST
    Sequence conflicti407 – 42519TFHTD…LMNES → IFTRTRFLYRFWPTNE in AAA34625. (PubMed:3062381)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21615 Genomic DNA. Translation: AAA34625.1.
    X95966 Genomic DNA. Translation: CAA65201.1.
    Z48008 Genomic DNA. Translation: CAA88069.1.
    Z74305 Genomic DNA. Translation: CAA98829.1.
    AY723766 Genomic DNA. Translation: AAU09683.1.
    BK006938 Genomic DNA. Translation: DAA11856.1.
    PIRiS50990.
    RefSeqiNP_010292.1. NM_001180317.1.

    Genome annotation databases

    EnsemblFungiiYDR009W; YDR009W; YDR009W.
    GeneIDi851572.
    KEGGisce:YDR009W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21615 Genomic DNA. Translation: AAA34625.1 .
    X95966 Genomic DNA. Translation: CAA65201.1 .
    Z48008 Genomic DNA. Translation: CAA88069.1 .
    Z74305 Genomic DNA. Translation: CAA98829.1 .
    AY723766 Genomic DNA. Translation: AAU09683.1 .
    BK006938 Genomic DNA. Translation: DAA11856.1 .
    PIRi S50990.
    RefSeqi NP_010292.1. NM_001180317.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3V2U X-ray 2.10 C/D 2-520 [» ]
    3V5R X-ray 2.10 A/B 17-520 [» ]
    ProteinModelPortali P13045.
    SMRi P13045. Positions 2-520.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32061. 47 interactions.
    DIPi DIP-5725N.
    IntActi P13045. 2 interactions.
    MINTi MINT-4480213.
    STRINGi 4932.YDR009W.

    Proteomic databases

    MaxQBi P13045.
    PaxDbi P13045.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR009W ; YDR009W ; YDR009W .
    GeneIDi 851572.
    KEGGi sce:YDR009W.

    Organism-specific databases

    CYGDi YDR009w.
    SGDi S000002416. GAL3.

    Phylogenomic databases

    eggNOGi COG0153.
    GeneTreei ENSGT00530000063433.
    HOGENOMi HOG000241099.
    KOi K00849.
    OMAi CREAGAY.
    OrthoDBi EOG7FZ075.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29629-MONOMER.

    Miscellaneous databases

    NextBioi 969025.

    Gene expression databases

    Genevestigatori P13045.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.890. 2 hits.
    InterProi IPR000705. Galactokinase.
    IPR019741. Galactokinase_CS.
    IPR019539. GalKase_gal-bd.
    IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006206. Mevalonate/galactokinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    Pfami PF10509. GalKase_gal_bdg. 1 hit.
    PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000530. Galactokinase. 1 hit.
    PRINTSi PR00473. GALCTOKINASE.
    PR00959. MEVGALKINASE.
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 2 hits.
    TIGRFAMsi TIGR00131. gal_kin. 1 hit.
    PROSITEi PS00106. GALACTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases."
      Bajwa W., Torchia T.E., Hopper J.E.
      Mol. Cell. Biol. 8:3439-3447(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-425.
    2. "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames."
      Eide L.G., Sander C., Prydz H.
      Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGAL3_YEAST
    AccessioniPrimary (citable) accession number: P13045
    Secondary accession number(s): D6VRZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 721 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3