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Protein

Protein GAL3

Gene

GAL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.

GO - Molecular functioni

GO - Biological processi

  • galactose metabolic process Source: SGD
  • maintenance of protein location Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29629-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GAL3
Gene namesi
Name:GAL3
Ordered Locus Names:YDR009W
ORF Names:YD8119.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR009w.
EuPathDBiFungiDB:YDR009W.
SGDiS000002416. GAL3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Protein GAL3PRO_0000184658Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP13045.
PaxDbiP13045.

Expressioni

Inductioni

The GAL3 gene is a member of the family of galactose inducible, glucose-repressible genes (which include GAL1, GAL2, GAL7, GAL10, GAL80, and MELI).

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
URE2P232021EBI-7282,EBI-20138

Protein-protein interaction databases

BioGridi32061. 48 interactions.
DIPiDIP-5725N.
IntActiP13045. 2 interactions.
MINTiMINT-4480213.
STRINGi4932.YDR009W.

Structurei

Secondary structure

1
520
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Helixi20 – 3415Combined sources
Beta strandi39 – 5113Combined sources
Helixi56 – 583Combined sources
Beta strandi62 – 7716Combined sources
Beta strandi84 – 918Combined sources
Beta strandi97 – 1004Combined sources
Beta strandi112 – 1143Combined sources
Helixi119 – 13517Combined sources
Helixi137 – 1404Combined sources
Beta strandi141 – 1433Combined sources
Beta strandi148 – 1547Combined sources
Helixi162 – 17918Combined sources
Helixi187 – 1959Combined sources
Helixi198 – 2014Combined sources
Helixi208 – 2158Combined sources
Beta strandi220 – 2256Combined sources
Beta strandi227 – 2293Combined sources
Beta strandi231 – 2366Combined sources
Beta strandi244 – 2518Combined sources
Helixi258 – 2614Combined sources
Turni263 – 2653Combined sources
Helixi266 – 28318Combined sources
Beta strandi296 – 2994Combined sources
Helixi302 – 31312Combined sources
Helixi324 – 34219Combined sources
Helixi343 – 3453Combined sources
Helixi351 – 3577Combined sources
Helixi362 – 3698Combined sources
Beta strandi370 – 3734Combined sources
Beta strandi378 – 3803Combined sources
Helixi382 – 40423Combined sources
Helixi411 – 43121Combined sources
Helixi438 – 44912Combined sources
Beta strandi453 – 47321Combined sources
Helixi478 – 48811Combined sources
Helixi490 – 4934Combined sources
Helixi499 – 5057Combined sources
Beta strandi506 – 5094Combined sources
Beta strandi515 – 5195Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V2UX-ray2.10C/D2-520[»]
3V5RX-ray2.10A/B17-520[»]
ProteinModelPortaliP13045.
SMRiP13045. Positions 2-520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.Curated

Phylogenomic databases

eggNOGiCOG0153.
GeneTreeiENSGT00530000063433.
HOGENOMiHOG000241099.
InParanoidiP13045.
KOiK00849.
OMAiSIKSERY.
OrthoDBiEOG7FZ075.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTNVPIFSS PVRDLPRSFE QKHLAVVDAF FQTYHVKPDF IARSPGRVNL
60 70 80 90 100
IGEHIDYCDF SVLPLAIDVD MLCAVKILDE KNPSITLTNA DPKFAQRKFD
110 120 130 140 150
LPLDGSYMAI DPSVSEWSNY FKCGLHVAHS YLKKIAPERF NNTPLVGAQI
160 170 180 190 200
FCQSDIPTGG GLSSAFTCAA ALATIRANMG KNFDISKKDL TRITAVAEHY
210 220 230 240 250
VGVNNGGMDQ ATSVYGEEDH ALYVEFRPKL KATPFKFPQL KNHEISFVIA
260 270 280 290 300
NTLVKSNKFE TAPTNYNLRV IEVTVAANAL ATRYSVALPS HKDNSNSERG
310 320 330 340 350
NLRDFMDAYY ARYENQAQPW NGDIGTGIER LLKMLQLVEE SFSRKKSGFT
360 370 380 390 400
VHEASTALNC SREEFTRDYL TTFPVRFQVL KLYQRAKHVY SESLRVLKAL
410 420 430 440 450
KMMTSATFHT DEDFFTDFGR LMNESQASCD KLYECSCIET NQICSIALAN
460 470 480 490 500
GSFGSRLTGA GWGGCTIHLV PSGANGNVEQ VRKALIEKFY NVRYPDLTDE
510 520
ELKDAIIVSK PALGTCLYEQ
Length:520
Mass (Da):58,129
Last modified:February 1, 1996 - v2
Checksum:i5852028EBDDC0F3B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 459KPDFIARSP → NLILSLGLL in AAA34625 (PubMed:3062381).Curated
Sequence conflicti170 – 1712AA → GR in AAA34625 (PubMed:3062381).Curated
Sequence conflicti192 – 21120RITAV…GMDQA → ASQRLRSTMLESIMVVWIKQ in AAA34625 (PubMed:3062381).CuratedAdd
BLAST
Sequence conflicti227 – 24115RPKLK…FPQLK → MAKTKWPHFQVSSIE in AAA34625 (PubMed:3062381).CuratedAdd
BLAST
Sequence conflicti251 – 29444NTLVK…SHKDN → ILCTRSNNRTLLHISLSCSL LTLLYISTFAREPCGPDTLG LTISQGQ in AAA34625 (PubMed:3062381).CuratedAdd
BLAST
Sequence conflicti303 – 33836RDFMD…MLQLV → EIYGCLLRPDTKTKPNHGME ISELVLNVYSRCYNWY in AAA34625 (PubMed:3062381).CuratedAdd
BLAST
Sequence conflicti407 – 42519TFHTD…LMNES → IFTRTRFLYRFWPTNE in AAA34625 (PubMed:3062381).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21615 Genomic DNA. Translation: AAA34625.1.
X95966 Genomic DNA. Translation: CAA65201.1.
Z48008 Genomic DNA. Translation: CAA88069.1.
Z74305 Genomic DNA. Translation: CAA98829.1.
AY723766 Genomic DNA. Translation: AAU09683.1.
BK006938 Genomic DNA. Translation: DAA11856.1.
PIRiS50990.
RefSeqiNP_010292.1. NM_001180317.1.

Genome annotation databases

EnsemblFungiiYDR009W; YDR009W; YDR009W.
GeneIDi851572.
KEGGisce:YDR009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21615 Genomic DNA. Translation: AAA34625.1.
X95966 Genomic DNA. Translation: CAA65201.1.
Z48008 Genomic DNA. Translation: CAA88069.1.
Z74305 Genomic DNA. Translation: CAA98829.1.
AY723766 Genomic DNA. Translation: AAU09683.1.
BK006938 Genomic DNA. Translation: DAA11856.1.
PIRiS50990.
RefSeqiNP_010292.1. NM_001180317.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V2UX-ray2.10C/D2-520[»]
3V5RX-ray2.10A/B17-520[»]
ProteinModelPortaliP13045.
SMRiP13045. Positions 2-520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32061. 48 interactions.
DIPiDIP-5725N.
IntActiP13045. 2 interactions.
MINTiMINT-4480213.
STRINGi4932.YDR009W.

Proteomic databases

MaxQBiP13045.
PaxDbiP13045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR009W; YDR009W; YDR009W.
GeneIDi851572.
KEGGisce:YDR009W.

Organism-specific databases

CYGDiYDR009w.
EuPathDBiFungiDB:YDR009W.
SGDiS000002416. GAL3.

Phylogenomic databases

eggNOGiCOG0153.
GeneTreeiENSGT00530000063433.
HOGENOMiHOG000241099.
InParanoidiP13045.
KOiK00849.
OMAiSIKSERY.
OrthoDBiEOG7FZ075.

Enzyme and pathway databases

BioCyciYEAST:G3O-29629-MONOMER.

Miscellaneous databases

NextBioi969025.
PROiP13045.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases."
    Bajwa W., Torchia T.E., Hopper J.E.
    Mol. Cell. Biol. 8:3439-3447(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-425.
  2. "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames."
    Eide L.G., Sander C., Prydz H.
    Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGAL3_YEAST
AccessioniPrimary (citable) accession number: P13045
Secondary accession number(s): D6VRZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.