Skip Header

Contribute Send feedback
Read comments (?) or add your own

P13036 (FECA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fe(3+) dicitrate transport protein FecA
Alternative name(s):
Iron(III) dicitrate transport protein FecA
Gene names
Name:fecA
Ordered Locus Names:b4291, JW4251
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length774 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

FecA is the outer membrane receptor protein in the Fe3+ dicitrate transport system.

Subcellular location

Cell outer membrane.

Induction

For induction, the TonB and the ExbB proteins have to be active. Regulation by the iron level mediated by the Fur protein and induction by citrate plus iron suggest that the Fe3+ dicitrate complex must enter the periplasm where it binds to a transmembrane protein, which regulates transcription of the fec genes directly or via a further inductor.

Sequence similarities

Belongs to the TonB-dependent receptor family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Ref.1
Chain34 – 774741Fe(3+) dicitrate transport protein FecA
PRO_0000034746

Regions

Motif56 – 638TonB box
Motif757 – 77418TonB C-terminal box

Experimental info

Sequence conflict161T → A in AAA23760. Ref.1
Sequence conflict1901A → T in AAA23760. Ref.1
Sequence conflict3001T → M Ref.1
Sequence conflict3001T → M Ref.5
Sequence conflict3571W → R in AAA23760. Ref.1
Sequence conflict4441L → V in AAA23760. Ref.1
Sequence conflict7491R → A in AAA23760. Ref.1

Secondary structure

1 .................................................................................................................. 774
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13036 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 16B5B510276C3B09

FASTA77485,322
        10         20         30         40         50         60 
MTPLRVFRKT TPLVNTIRLS LLPLAGLSFS AFAAQVNIAP GSLDKALNQY AAHSGFTLSV 

        70         80         90        100        110        120 
DASLTRGKQS NGLHGDYDVE SGLQQLLDGS GLQVKPLGNN SWTLEPAPAP KEDALTVVGD 

       130        140        150        160        170        180 
WLGDARENDV FEHAGARDVI RREDFAKTGA TTMREVLNRI PGVSAPENNG TGSHDLAMNF 

       190        200        210        220        230        240 
GIRGLNPRLA SRSTVLMDGI PVPFAPYGQP QLSLAPVSLG NMDAIDVVRG GGAVRYGPQS 

       250        260        270        280        290        300 
VGGVVNFVTR AIPQDFGIEA GVEGQLSPTS SQNNPKETHN LMVGGTADNG FGTALLYSGT 

       310        320        330        340        350        360 
RGSDWREHSA TRIDDLMLKS KYAPDEVHTF NSLLQYYDGE ADMPGGLSRA DYDADRWQST 

       370        380        390        400        410        420 
RPYDRFWGRR KLASLGYQFQ PDSQHKFNIQ GFYTQTLRSG YLEQGKRITL SPRNYWVRGI 

       430        440        450        460        470        480 
EPRYSQIFMI GPSAHEVGVG YRYLNESTHE MRYYTATSSG QLPSGSSPYD RDTRSGTEAH 

       490        500        510        520        530        540 
AWYLDDKIDI GNWTITPGMR FEHIESYQNN AITGTHEEVS YNAPLPALNV LYHLTDSWNL 

       550        560        570        580        590        600 
YANTEGSFGT VQYSQIGKAV QSGNVEPEKA RTWELGTRYD DGALTAEMGL FLINFNNQYD 

       610        620        630        640        650        660 
SNQTNDTVTA RGKTRHTGLE TQARYDLGTL TPTLDNVSIY ASYAYVNAEI REKGDTYGNL 

       670        680        690        700        710        720 
VPFSPKHKGT LGVDYKPGNW TFNLNSDFQS SQFADNANTV KESADGSTGR IPGFMLWGAR 

       730        740        750        760        770 
VAYDFGPQMA DLNLAFGVKN IFDQDYFIRS YDDNNKGIYA GQPRTLYMQG SLKF

« Hide

References

« Hide 'large scale' references
[1]"Genetics of the iron dicitrate transport system of Escherichia coli."
Pressler U., Staudenmaier H., Zimmermann L., Braun V.
J. Bacteriol. 170:2716-2724(1988) [PubMed: 2836368] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 34-40.
Strain: B.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Novel two-component transmembrane transcription control: regulation of iron dicitrate transport in Escherichia coli K-12."
van Hove B., Staudenmaier H., Braun V.
J. Bacteriol. 172:6749-6758(1990) [PubMed: 2254251] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-428.
[6]"Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli."
Staudenmaier H., van Hove B., Yaraghi Z., Braun V.
J. Bacteriol. 171:2626-2633(1989) [PubMed: 2651410] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 731-774.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M20981 Genomic DNA. Translation: AAA23760.1.
U14003 Genomic DNA. Translation: AAA97187.1.
U00096 Genomic DNA. Translation: AAC77247.1.
AP009048 Genomic DNA. Translation: BAE78282.1.
M63115 Genomic DNA. Translation: AAA23768.1.
M26397 Genomic DNA. Translation: AAA23761.1.
PIRQRECFA. E65242.
RefSeqNP_418711.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KMOX-ray2.00A1-774[»]
1KMPX-ray2.50A1-774[»]
1PNZX-ray2.50A34-774[»]
1PO0X-ray2.15A34-774[»]
1PO3X-ray3.40A/B34-774[»]
1ZZVNMR-A34-113[»]
2D1UNMR-A34-129[»]
ProteinModelPortalP13036.
SMRP13036. Positions 34-774.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9583N.
IntActP13036. 3 interactions.

Proteomic databases

PRIDEP13036.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002700; EBESCP00000002700; EBESCG00000002201.
EBESCT00000018226; EBESCP00000017517; EBESCG00000017281.
GeneID946427.
GenomeReviewsGene locus JW4251 in contig AP009048_GR.
Gene locus b4291 in contig U00096_GR.
KEGGecj:JW4251.
eco:b4291.
PATRIC32124161. VBIEscCol129921_4424.

Organism-specific databases

EchoBASEEB0282.
EcoGeneEG10286. fecA.

Phylogenomic databases

eggNOGCOG4772.
GeneTreeEBGT00070000031689.
HOGENOMHBG337249.
OMAGNMDAVD.
PhylomeDBP13036.
ProtClustDBCLSK867865.

Enzyme and pathway databases

BioCycEcoCyc:EG10286-MONOMER.

Gene expression databases

GenevestigatorP13036.

Family and domain databases

InterProIPR012910. Plug.
IPR011662. Secretin/TonB_short_N.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
Gene3DG3DSA:2.170.130.10. Plug. 1 hit.
G3DSA:2.40.170.20. TonB-dep_rcpt_b-brl. 1 hit.
KOK02014.
PfamPF07715. Plug. 1 hit.
PF07660. STN. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
SMARTSM00965. STN. 1 hit.
[Graphical view]
TIGRFAMsTIGR01783. TonB-siderophor. 1 hit.
PROSITEPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFECA_ECOLI
AccessionPrimary (citable) accession number: P13036
Secondary accession number(s): Q2M624
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1995
Last modified: January 25, 2012
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents