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Protein

Fe(3+) dicitrate transport protein FecA

Gene

fecA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

FecA is the outer membrane receptor protein in the Fe3+ dicitrate transport system.

GO - Molecular functioni

  • ferric iron transmembrane transporter activity Source: EcoCyc
  • iron ion binding Source: InterPro
  • receptor activity Source: InterPro

GO - Biological processi

  • ferric iron transport Source: EcoCyc
  • iron ion homeostasis Source: UniProtKB-KW
  • siderophore transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:EG10286-MONOMER.
ECOL316407:JW4251-MONOMER.
MetaCyc:EG10286-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.20. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Fe(3+) dicitrate transport protein FecA
Alternative name(s):
Iron(III) dicitrate transport protein FecA
Gene namesi
Name:fecA
Ordered Locus Names:b4291, JW4251
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10286. fecA.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000003474634 – 774Fe(3+) dicitrate transport protein FecAAdd BLAST741

Proteomic databases

PaxDbiP13036.
PRIDEiP13036.

Expressioni

Inductioni

For induction, the TonB and the ExbB proteins have to be active. Regulation by the iron level mediated by the Fur protein and induction by citrate plus iron suggest that the Fe3+ dicitrate complex must enter the periplasm where it binds to a transmembrane protein, which regulates transcription of the fec genes directly or via a further inductor. Induced 2-fold by hydroxyurea (PubMed:20005847).1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261554. 298 interactors.
DIPiDIP-9583N.
IntActiP13036. 3 interactors.
MINTiMINT-119307.
STRINGi511145.b4291.

Structurei

Secondary structure

1774
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 38Combined sources3
Helixi43 – 54Combined sources12
Turni62 – 65Combined sources4
Beta strandi73 – 76Combined sources4
Helixi79 – 86Combined sources8
Turni87 – 89Combined sources3
Beta strandi93 – 98Combined sources6
Beta strandi101 – 105Combined sources5
Beta strandi108 – 110Combined sources3
Helixi127 – 131Combined sources5
Beta strandi137 – 141Combined sources5
Helixi142 – 144Combined sources3
Helixi146 – 148Combined sources3
Helixi153 – 157Combined sources5
Beta strandi174 – 177Combined sources4
Helixi188 – 190Combined sources3
Beta strandi192 – 197Combined sources6
Beta strandi203 – 205Combined sources3
Turni206 – 208Combined sources3
Helixi219 – 221Combined sources3
Beta strandi222 – 228Combined sources7
Turni233 – 235Combined sources3
Helixi240 – 242Combined sources3
Beta strandi244 – 249Combined sources6
Beta strandi257 – 266Combined sources10
Beta strandi276 – 286Combined sources11
Beta strandi290 – 302Combined sources13
Beta strandi305 – 308Combined sources4
Beta strandi311 – 322Combined sources12
Beta strandi324 – 342Combined sources19
Helixi349 – 354Combined sources6
Beta strandi365 – 379Combined sources15
Beta strandi385 – 404Combined sources20
Beta strandi407 – 410Combined sources4
Beta strandi413 – 430Combined sources18
Beta strandi433 – 456Combined sources24
Beta strandi459 – 461Combined sources3
Beta strandi465 – 467Combined sources3
Beta strandi469 – 490Combined sources22
Beta strandi493 – 510Combined sources18
Turni511 – 513Combined sources3
Beta strandi516 – 535Combined sources20
Beta strandi538 – 549Combined sources12
Helixi553 – 555Combined sources3
Helixi556 – 561Combined sources6
Beta strandi568 – 580Combined sources13
Beta strandi582 – 600Combined sources19
Beta strandi603 – 605Combined sources3
Beta strandi612 – 626Combined sources15
Helixi627 – 629Combined sources3
Helixi632 – 634Combined sources3
Beta strandi637 – 650Combined sources14
Beta strandi652 – 654Combined sources3
Turni655 – 658Combined sources4
Beta strandi666 – 675Combined sources10
Beta strandi680 – 689Combined sources10
Beta strandi706 – 709Combined sources4
Beta strandi714 – 724Combined sources11
Turni727 – 730Combined sources4
Beta strandi733 – 740Combined sources8
Beta strandi747 – 750Combined sources4
Beta strandi757 – 760Combined sources4
Beta strandi764 – 774Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KMOX-ray2.00A1-774[»]
1KMPX-ray2.50A1-774[»]
1PNZX-ray2.50A34-774[»]
1PO0X-ray2.15A34-774[»]
1PO3X-ray3.40A/B34-774[»]
1ZZVNMR-A34-113[»]
2D1UNMR-A34-129[»]
ProteinModelPortaliP13036.
SMRiP13036.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13036.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi56 – 63TonB box8
Motifi757 – 774TonB C-terminal boxAdd BLAST18

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105GAV. Bacteria.
COG4772. LUCA.
HOGENOMiHOG000219169.
InParanoidiP13036.
KOiK16091.
OMAiQYESNQT.
PhylomeDBiP13036.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR011662. Secretin/TonB_short_N.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF07660. STN. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
SMARTiSM00965. STN. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPLRVFRKT TPLVNTIRLS LLPLAGLSFS AFAAQVNIAP GSLDKALNQY
60 70 80 90 100
AAHSGFTLSV DASLTRGKQS NGLHGDYDVE SGLQQLLDGS GLQVKPLGNN
110 120 130 140 150
SWTLEPAPAP KEDALTVVGD WLGDARENDV FEHAGARDVI RREDFAKTGA
160 170 180 190 200
TTMREVLNRI PGVSAPENNG TGSHDLAMNF GIRGLNPRLA SRSTVLMDGI
210 220 230 240 250
PVPFAPYGQP QLSLAPVSLG NMDAIDVVRG GGAVRYGPQS VGGVVNFVTR
260 270 280 290 300
AIPQDFGIEA GVEGQLSPTS SQNNPKETHN LMVGGTADNG FGTALLYSGT
310 320 330 340 350
RGSDWREHSA TRIDDLMLKS KYAPDEVHTF NSLLQYYDGE ADMPGGLSRA
360 370 380 390 400
DYDADRWQST RPYDRFWGRR KLASLGYQFQ PDSQHKFNIQ GFYTQTLRSG
410 420 430 440 450
YLEQGKRITL SPRNYWVRGI EPRYSQIFMI GPSAHEVGVG YRYLNESTHE
460 470 480 490 500
MRYYTATSSG QLPSGSSPYD RDTRSGTEAH AWYLDDKIDI GNWTITPGMR
510 520 530 540 550
FEHIESYQNN AITGTHEEVS YNAPLPALNV LYHLTDSWNL YANTEGSFGT
560 570 580 590 600
VQYSQIGKAV QSGNVEPEKA RTWELGTRYD DGALTAEMGL FLINFNNQYD
610 620 630 640 650
SNQTNDTVTA RGKTRHTGLE TQARYDLGTL TPTLDNVSIY ASYAYVNAEI
660 670 680 690 700
REKGDTYGNL VPFSPKHKGT LGVDYKPGNW TFNLNSDFQS SQFADNANTV
710 720 730 740 750
KESADGSTGR IPGFMLWGAR VAYDFGPQMA DLNLAFGVKN IFDQDYFIRS
760 770
YDDNNKGIYA GQPRTLYMQG SLKF
Length:774
Mass (Da):85,322
Last modified:February 1, 1995 - v2
Checksum:i16B5B510276C3B09
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16T → A in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti190A → T in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti300T → M in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti300T → M (PubMed:2254251).Curated1
Sequence conflicti357W → R in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti444L → V in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti749R → A in AAA23760 (PubMed:2836368).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20981 Genomic DNA. Translation: AAA23760.1.
U14003 Genomic DNA. Translation: AAA97187.1.
U00096 Genomic DNA. Translation: AAC77247.1.
AP009048 Genomic DNA. Translation: BAE78282.1.
M63115 Genomic DNA. Translation: AAA23768.1.
M26397 Genomic DNA. Translation: AAA23761.1.
PIRiE65242. QRECFA.
RefSeqiNP_418711.1. NC_000913.3.
WP_000188283.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77247; AAC77247; b4291.
BAE78282; BAE78282; BAE78282.
GeneIDi946427.
KEGGiecj:JW4251.
eco:b4291.
PATRICi32124161. VBIEscCol129921_4424.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20981 Genomic DNA. Translation: AAA23760.1.
U14003 Genomic DNA. Translation: AAA97187.1.
U00096 Genomic DNA. Translation: AAC77247.1.
AP009048 Genomic DNA. Translation: BAE78282.1.
M63115 Genomic DNA. Translation: AAA23768.1.
M26397 Genomic DNA. Translation: AAA23761.1.
PIRiE65242. QRECFA.
RefSeqiNP_418711.1. NC_000913.3.
WP_000188283.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KMOX-ray2.00A1-774[»]
1KMPX-ray2.50A1-774[»]
1PNZX-ray2.50A34-774[»]
1PO0X-ray2.15A34-774[»]
1PO3X-ray3.40A/B34-774[»]
1ZZVNMR-A34-113[»]
2D1UNMR-A34-129[»]
ProteinModelPortaliP13036.
SMRiP13036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261554. 298 interactors.
DIPiDIP-9583N.
IntActiP13036. 3 interactors.
MINTiMINT-119307.
STRINGi511145.b4291.

Protein family/group databases

TCDBi1.B.14.1.20. the outer membrane receptor (omr) family.

Proteomic databases

PaxDbiP13036.
PRIDEiP13036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77247; AAC77247; b4291.
BAE78282; BAE78282; BAE78282.
GeneIDi946427.
KEGGiecj:JW4251.
eco:b4291.
PATRICi32124161. VBIEscCol129921_4424.

Organism-specific databases

EchoBASEiEB0282.
EcoGeneiEG10286. fecA.

Phylogenomic databases

eggNOGiENOG4105GAV. Bacteria.
COG4772. LUCA.
HOGENOMiHOG000219169.
InParanoidiP13036.
KOiK16091.
OMAiQYESNQT.
PhylomeDBiP13036.

Enzyme and pathway databases

BioCyciEcoCyc:EG10286-MONOMER.
ECOL316407:JW4251-MONOMER.
MetaCyc:EG10286-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP13036.
PROiP13036.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR011662. Secretin/TonB_short_N.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF07660. STN. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
SMARTiSM00965. STN. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFECA_ECOLI
AccessioniPrimary (citable) accession number: P13036
Secondary accession number(s): Q2M624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.