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Protein

Fe(3+) dicitrate transport protein FecA

Gene

fecA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

FecA is the outer membrane receptor protein in the Fe3+ dicitrate transport system.

GO - Molecular functioni

  • ferric iron transmembrane transporter activity Source: EcoCyc
  • iron ion binding Source: InterPro
  • receptor activity Source: InterPro

GO - Biological processi

  • ferric iron transport Source: EcoCyc
  • iron ion homeostasis Source: UniProtKB-KW
  • siderophore transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:EG10286-MONOMER.
ECOL316407:JW4251-MONOMER.
MetaCyc:EG10286-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.20. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Fe(3+) dicitrate transport protein FecA
Alternative name(s):
Iron(III) dicitrate transport protein FecA
Gene namesi
Name:fecA
Ordered Locus Names:b4291, JW4251
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10286. fecA.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 33331 PublicationAdd
BLAST
Chaini34 – 774741Fe(3+) dicitrate transport protein FecAPRO_0000034746Add
BLAST

Proteomic databases

PaxDbiP13036.
PRIDEiP13036.

Expressioni

Inductioni

For induction, the TonB and the ExbB proteins have to be active. Regulation by the iron level mediated by the Fur protein and induction by citrate plus iron suggest that the Fe3+ dicitrate complex must enter the periplasm where it binds to a transmembrane protein, which regulates transcription of the fec genes directly or via a further inductor. Induced 2-fold by hydroxyurea (PubMed:20005847).1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261554. 298 interactions.
DIPiDIP-9583N.
IntActiP13036. 3 interactions.
MINTiMINT-119307.
STRINGi511145.b4291.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 383Combined sources
Helixi43 – 5412Combined sources
Turni62 – 654Combined sources
Beta strandi73 – 764Combined sources
Helixi79 – 868Combined sources
Turni87 – 893Combined sources
Beta strandi93 – 986Combined sources
Beta strandi101 – 1055Combined sources
Beta strandi108 – 1103Combined sources
Helixi127 – 1315Combined sources
Beta strandi137 – 1415Combined sources
Helixi142 – 1443Combined sources
Helixi146 – 1483Combined sources
Helixi153 – 1575Combined sources
Beta strandi174 – 1774Combined sources
Helixi188 – 1903Combined sources
Beta strandi192 – 1976Combined sources
Beta strandi203 – 2053Combined sources
Turni206 – 2083Combined sources
Helixi219 – 2213Combined sources
Beta strandi222 – 2287Combined sources
Turni233 – 2353Combined sources
Helixi240 – 2423Combined sources
Beta strandi244 – 2496Combined sources
Beta strandi257 – 26610Combined sources
Beta strandi276 – 28611Combined sources
Beta strandi290 – 30213Combined sources
Beta strandi305 – 3084Combined sources
Beta strandi311 – 32212Combined sources
Beta strandi324 – 34219Combined sources
Helixi349 – 3546Combined sources
Beta strandi365 – 37915Combined sources
Beta strandi385 – 40420Combined sources
Beta strandi407 – 4104Combined sources
Beta strandi413 – 43018Combined sources
Beta strandi433 – 45624Combined sources
Beta strandi459 – 4613Combined sources
Beta strandi465 – 4673Combined sources
Beta strandi469 – 49022Combined sources
Beta strandi493 – 51018Combined sources
Turni511 – 5133Combined sources
Beta strandi516 – 53520Combined sources
Beta strandi538 – 54912Combined sources
Helixi553 – 5553Combined sources
Helixi556 – 5616Combined sources
Beta strandi568 – 58013Combined sources
Beta strandi582 – 60019Combined sources
Beta strandi603 – 6053Combined sources
Beta strandi612 – 62615Combined sources
Helixi627 – 6293Combined sources
Helixi632 – 6343Combined sources
Beta strandi637 – 65014Combined sources
Beta strandi652 – 6543Combined sources
Turni655 – 6584Combined sources
Beta strandi666 – 67510Combined sources
Beta strandi680 – 68910Combined sources
Beta strandi706 – 7094Combined sources
Beta strandi714 – 72411Combined sources
Turni727 – 7304Combined sources
Beta strandi733 – 7408Combined sources
Beta strandi747 – 7504Combined sources
Beta strandi757 – 7604Combined sources
Beta strandi764 – 77411Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KMOX-ray2.00A1-774[»]
1KMPX-ray2.50A1-774[»]
1PNZX-ray2.50A34-774[»]
1PO0X-ray2.15A34-774[»]
1PO3X-ray3.40A/B34-774[»]
1ZZVNMR-A34-113[»]
2D1UNMR-A34-129[»]
ProteinModelPortaliP13036.
SMRiP13036. Positions 34-774.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13036.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi56 – 638TonB box
Motifi757 – 77418TonB C-terminal boxAdd
BLAST

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105GAV. Bacteria.
COG4772. LUCA.
HOGENOMiHOG000219169.
InParanoidiP13036.
KOiK16091.
OMAiQYESNQT.
PhylomeDBiP13036.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR011662. Secretin/TonB_short_N.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF07660. STN. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
SMARTiSM00965. STN. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPLRVFRKT TPLVNTIRLS LLPLAGLSFS AFAAQVNIAP GSLDKALNQY
60 70 80 90 100
AAHSGFTLSV DASLTRGKQS NGLHGDYDVE SGLQQLLDGS GLQVKPLGNN
110 120 130 140 150
SWTLEPAPAP KEDALTVVGD WLGDARENDV FEHAGARDVI RREDFAKTGA
160 170 180 190 200
TTMREVLNRI PGVSAPENNG TGSHDLAMNF GIRGLNPRLA SRSTVLMDGI
210 220 230 240 250
PVPFAPYGQP QLSLAPVSLG NMDAIDVVRG GGAVRYGPQS VGGVVNFVTR
260 270 280 290 300
AIPQDFGIEA GVEGQLSPTS SQNNPKETHN LMVGGTADNG FGTALLYSGT
310 320 330 340 350
RGSDWREHSA TRIDDLMLKS KYAPDEVHTF NSLLQYYDGE ADMPGGLSRA
360 370 380 390 400
DYDADRWQST RPYDRFWGRR KLASLGYQFQ PDSQHKFNIQ GFYTQTLRSG
410 420 430 440 450
YLEQGKRITL SPRNYWVRGI EPRYSQIFMI GPSAHEVGVG YRYLNESTHE
460 470 480 490 500
MRYYTATSSG QLPSGSSPYD RDTRSGTEAH AWYLDDKIDI GNWTITPGMR
510 520 530 540 550
FEHIESYQNN AITGTHEEVS YNAPLPALNV LYHLTDSWNL YANTEGSFGT
560 570 580 590 600
VQYSQIGKAV QSGNVEPEKA RTWELGTRYD DGALTAEMGL FLINFNNQYD
610 620 630 640 650
SNQTNDTVTA RGKTRHTGLE TQARYDLGTL TPTLDNVSIY ASYAYVNAEI
660 670 680 690 700
REKGDTYGNL VPFSPKHKGT LGVDYKPGNW TFNLNSDFQS SQFADNANTV
710 720 730 740 750
KESADGSTGR IPGFMLWGAR VAYDFGPQMA DLNLAFGVKN IFDQDYFIRS
760 770
YDDNNKGIYA GQPRTLYMQG SLKF
Length:774
Mass (Da):85,322
Last modified:February 1, 1995 - v2
Checksum:i16B5B510276C3B09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161T → A in AAA23760 (PubMed:2836368).Curated
Sequence conflicti190 – 1901A → T in AAA23760 (PubMed:2836368).Curated
Sequence conflicti300 – 3001T → M in AAA23760 (PubMed:2836368).Curated
Sequence conflicti300 – 3001T → M (PubMed:2254251).Curated
Sequence conflicti357 – 3571W → R in AAA23760 (PubMed:2836368).Curated
Sequence conflicti444 – 4441L → V in AAA23760 (PubMed:2836368).Curated
Sequence conflicti749 – 7491R → A in AAA23760 (PubMed:2836368).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20981 Genomic DNA. Translation: AAA23760.1.
U14003 Genomic DNA. Translation: AAA97187.1.
U00096 Genomic DNA. Translation: AAC77247.1.
AP009048 Genomic DNA. Translation: BAE78282.1.
M63115 Genomic DNA. Translation: AAA23768.1.
M26397 Genomic DNA. Translation: AAA23761.1.
PIRiE65242. QRECFA.
RefSeqiNP_418711.1. NC_000913.3.
WP_000188283.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77247; AAC77247; b4291.
BAE78282; BAE78282; BAE78282.
GeneIDi946427.
KEGGiecj:JW4251.
eco:b4291.
PATRICi32124161. VBIEscCol129921_4424.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20981 Genomic DNA. Translation: AAA23760.1.
U14003 Genomic DNA. Translation: AAA97187.1.
U00096 Genomic DNA. Translation: AAC77247.1.
AP009048 Genomic DNA. Translation: BAE78282.1.
M63115 Genomic DNA. Translation: AAA23768.1.
M26397 Genomic DNA. Translation: AAA23761.1.
PIRiE65242. QRECFA.
RefSeqiNP_418711.1. NC_000913.3.
WP_000188283.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KMOX-ray2.00A1-774[»]
1KMPX-ray2.50A1-774[»]
1PNZX-ray2.50A34-774[»]
1PO0X-ray2.15A34-774[»]
1PO3X-ray3.40A/B34-774[»]
1ZZVNMR-A34-113[»]
2D1UNMR-A34-129[»]
ProteinModelPortaliP13036.
SMRiP13036. Positions 34-774.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261554. 298 interactions.
DIPiDIP-9583N.
IntActiP13036. 3 interactions.
MINTiMINT-119307.
STRINGi511145.b4291.

Protein family/group databases

TCDBi1.B.14.1.20. the outer membrane receptor (omr) family.

Proteomic databases

PaxDbiP13036.
PRIDEiP13036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77247; AAC77247; b4291.
BAE78282; BAE78282; BAE78282.
GeneIDi946427.
KEGGiecj:JW4251.
eco:b4291.
PATRICi32124161. VBIEscCol129921_4424.

Organism-specific databases

EchoBASEiEB0282.
EcoGeneiEG10286. fecA.

Phylogenomic databases

eggNOGiENOG4105GAV. Bacteria.
COG4772. LUCA.
HOGENOMiHOG000219169.
InParanoidiP13036.
KOiK16091.
OMAiQYESNQT.
PhylomeDBiP13036.

Enzyme and pathway databases

BioCyciEcoCyc:EG10286-MONOMER.
ECOL316407:JW4251-MONOMER.
MetaCyc:EG10286-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP13036.
PROiP13036.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR011662. Secretin/TonB_short_N.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF07660. STN. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
SMARTiSM00965. STN. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFECA_ECOLI
AccessioniPrimary (citable) accession number: P13036
Secondary accession number(s): Q2M624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.